##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2919457
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41592083733379	33.0	31.0	34.0	31.0	34.0
2	32.713020263699725	34.0	31.0	34.0	31.0	34.0
3	33.02968394465135	34.0	31.0	34.0	31.0	34.0
4	35.96518325154301	37.0	35.0	37.0	35.0	37.0
5	36.34118433667631	37.0	37.0	37.0	35.0	37.0
6	36.352924876098534	37.0	37.0	37.0	35.0	37.0
7	36.5173431223683	37.0	37.0	37.0	35.0	37.0
8	36.61593542908835	37.0	37.0	37.0	35.0	37.0
9	38.69686588978704	39.0	39.0	39.0	39.0	39.0
10	38.13095825696354	39.0	39.0	39.0	37.0	39.0
11	37.917971389885174	39.0	38.0	39.0	35.0	39.0
12	37.353971988626654	39.0	37.0	39.0	35.0	39.0
13	37.22095444461076	39.0	37.0	39.0	35.0	39.0
14	37.96225428221755	40.0	38.0	40.0	34.0	40.0
15	37.85191321536848	40.0	37.0	40.0	34.0	40.0
16	32.70863520168305	39.0	34.0	40.0	3.0	40.0
17	34.39266617045567	39.0	34.0	40.0	17.0	40.0
18	36.40813788317485	39.0	34.0	40.0	30.0	40.0
19	37.166568646155774	39.0	36.0	40.0	32.0	40.0
20	37.500880129421326	39.0	36.0	40.0	33.0	40.0
21	37.76017663558669	40.0	37.0	40.0	33.0	40.0
22	37.79763291598403	40.0	37.0	40.0	34.0	40.0
23	37.77978815923646	40.0	37.0	40.0	34.0	40.0
24	37.771703094102776	40.0	36.0	40.0	34.0	40.0
25	37.7166952621669	40.0	36.0	40.0	34.0	40.0
26	37.5971737210036	40.0	36.0	40.0	34.0	40.0
27	37.510323666353024	40.0	36.0	40.0	34.0	40.0
28	37.35778708163881	40.0	35.0	40.0	33.0	40.0
29	37.16835664988387	40.0	35.0	40.0	33.0	40.0
30	37.14820255958557	39.0	35.0	40.0	33.0	40.0
31	37.10151476798597	39.0	35.0	40.0	32.0	40.0
32	36.94918609864779	39.0	35.0	40.0	32.0	40.0
33	36.600324991941996	39.0	35.0	40.0	31.0	40.0
34	36.399377007436655	39.0	35.0	40.0	30.0	40.0
35	36.38246050549811	39.0	35.0	40.0	30.0	40.0
36	36.4199383652508	39.0	35.0	40.0	31.0	40.0
37	36.1474548177966	38.0	35.0	40.0	30.0	40.0
38	36.18987674762807	38.0	35.0	40.0	30.0	40.0
39	35.82925591985085	38.0	35.0	40.0	30.0	40.0
40	35.72866118596712	38.0	35.0	40.0	29.0	40.0
41	35.68675407789873	38.0	35.0	40.0	29.0	40.0
42	35.70163938019981	38.0	35.0	40.0	30.0	40.0
43	35.59989957036531	38.0	34.0	40.0	30.0	40.0
44	35.87363437789973	38.0	35.0	40.0	30.0	40.0
45	35.96991084300951	38.0	35.0	40.0	31.0	40.0
46	35.8890112099613	38.0	35.0	40.0	31.0	40.0
47	35.59573338466708	37.0	35.0	40.0	30.0	40.0
48	35.56345580702165	37.0	35.0	40.0	30.0	40.0
49	35.5140123659982	37.0	35.0	40.0	30.0	40.0
50	34.99005842524826	36.0	34.0	40.0	28.0	40.0
51	35.092351420144226	36.0	34.0	40.0	29.0	40.0
52	34.72865296526032	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	9.0
15	25.0
16	69.0
17	147.0
18	341.0
19	650.0
20	1347.0
21	2318.0
22	4064.0
23	6401.0
24	9421.0
25	13459.0
26	18658.0
27	24676.0
28	32030.0
29	39708.0
30	50810.0
31	66144.0
32	91372.0
33	141826.0
34	219563.0
35	259120.0
36	307544.0
37	527339.0
38	748189.0
39	354222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.54025697244386	0.3489690034824969	0.07559624957654797	0.03517777449710682
8	99.26164351795556	0.5779499406910258	0.07590452608139117	0.08450201527201805
9	97.6199683708306	1.2914387846781097	0.4485765674918315	0.6400162769994557
10	54.546753043459795	28.19990155703612	4.885292025195096	12.368053374308989
11	35.71099009165061	21.004042875096292	19.817075572615046	23.467891460638057
12	31.99851890265895	14.563701400637175	28.66577586174415	24.772003834959722
13	26.564939987127744	18.312275193640463	26.966315996433583	28.156468822798214
14	28.85019371753035	16.007531537542768	31.105818650523027	24.036456094403857
15	33.230597333682255	16.76709059253142	27.674701151618265	22.327610922168063
16	32.325531768407615	27.43075167745235	21.262412839099873	18.98130371504016
17	33.744425761365896	19.191479785453254	21.420387421359518	25.64370703182133
18	33.53736670894622	19.13626403814134	25.77153902249631	21.554830230416137
19	33.54421729794274	21.132970960010713	23.278643939609317	22.044167802437233
20	33.892329977800664	23.282617281227296	21.405864172686904	21.41918856828513
21	34.597940644441756	19.910791630087378	24.52325894849624	20.96800877697462
22	31.659449000276425	18.395955138232896	25.686317695379657	24.258278166111026
23	32.302239765819465	20.769478707855605	23.45302568251562	23.47525584380931
24	32.739067573182275	20.260411439524542	22.401151995045655	24.59936899224753
25	28.082037173351072	18.698066112979227	21.960761881404657	31.25913483226504
26	27.69391705375349	19.044911433872805	25.413321723868513	27.84784978850519
27	26.161680065847857	18.036264962970854	24.105818307993577	31.69623666318771
28	25.813909915439755	24.663867287649722	21.77829644348247	27.74392635342805
29	32.27394683326386	21.166949881433432	21.349826354695413	25.2092769306073
30	29.837740374323037	20.367451892595096	24.462699741767047	25.33210799131482
31	34.47839786645257	18.784760316730132	22.370838138736072	24.36600367808123
32	36.120038760632546	17.930320604139744	23.685568926002336	22.26407170922538
33	36.15093491700683	18.041060375268415	23.91715308702954	21.890851620695216
34	29.15018100968776	20.83709402125121	27.665247338803074	22.347477630257956
35	26.869859703362646	27.404102886255906	25.868166580292158	19.857870830089293
36	29.83332174442028	28.022539807916335	20.928686396134623	21.21545205152876
37	31.427214033294547	27.108671235781173	19.638035429191113	21.826079301733163
38	31.664552689078825	25.958765619771075	19.603439954758713	22.77324173639139
39	30.831486814157564	23.15574437301183	19.503524114244534	26.50924469858607
40	28.207539963767235	23.10535829094246	20.636680040158154	28.050421705132152
41	24.474996549015792	24.341204545913847	20.81736432494125	30.366434580129116
42	25.07969803973821	21.496600223945755	22.594064581187528	30.829637155128502
43	26.110095130704096	19.108587658595415	24.954572031716857	29.82674517898363
44	26.80772486116425	20.33641872444088	23.144338142332632	29.711518272062236
45	27.93238605672219	20.274009858682625	22.133636494731725	29.659967589863456
46	25.576982294995265	22.233004288126182	24.63393021373495	27.5560832031436
47	27.70720719640673	20.22259618826378	25.376534060957223	26.693662554372267
48	27.898578400024387	20.269180193440082	26.188431615879253	25.643809790656274
49	29.56988234455928	19.47132634596091	24.614645805709763	26.34414550377005
50	25.934754305338288	20.41444693311119	25.097030029899397	28.553768731651125
51	24.38854211587977	21.774254595974526	26.265466489145073	27.571736799000636
52	25.082643793006714	20.97489361891612	25.76732591026345	28.175136677813718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.0
6	8.0
7	6.5
8	5.0
9	40.0
10	75.0
11	309.5
12	544.0
13	869.0
14	1824.5
15	2455.0
16	2594.5
17	2734.0
18	3182.0
19	3630.0
20	3906.0
21	4182.0
22	4416.5
23	4651.0
24	4102.5
25	3554.0
26	5724.5
27	7895.0
28	9414.0
29	10933.0
30	11601.5
31	12270.0
32	15330.5
33	18391.0
34	20633.5
35	22876.0
36	25827.5
37	28779.0
38	35273.0
39	39369.5
40	36972.0
41	46648.0
42	56324.0
43	58198.5
44	60073.0
45	70186.5
46	80300.0
47	88210.0
48	96120.0
49	110484.5
50	124849.0
51	125316.0
52	125783.0
53	137009.0
54	148235.0
55	181171.5
56	214108.0
57	244502.5
58	274897.0
59	293993.5
60	313090.0
61	303209.5
62	293329.0
63	304159.0
64	266799.0
65	218609.0
66	179747.5
67	140886.0
68	113917.5
69	86949.0
70	74591.0
71	62233.0
72	54427.5
73	46622.0
74	39777.0
75	32932.0
76	24530.5
77	16129.0
78	11044.0
79	5959.0
80	4630.5
81	3302.0
82	1909.5
83	517.0
84	364.0
85	211.0
86	132.0
87	53.0
88	36.0
89	15.5
90	12.0
91	9.0
92	6.0
93	6.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2919457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.105812657784512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56574677038435	5.638869107196135
2	10.399879516583558	1.6859895004991374
3	4.449404481479946	1.0819811749674983
4	2.260455024965451	0.7329129981487023
5	1.3431157193557643	0.5443522199411651
6	0.8198292309282205	0.3987229294367828
7	0.5690439138042446	0.3228794351544779
8	0.3880493133009828	0.2516364028479755
9	0.29146037148309123	0.21262708515691953
>10	3.968512259680131	8.608529335475911
>50	2.887288607355793	17.08419868432294
>100	2.8533033706237227	41.080414903283476
>500	0.13933947037076883	7.7476602547104285
>1k	0.05728939896777255	8.34898929094215
>5k	0.004855033810828182	2.7376606286295644
>10k+	0.002427516905414091	3.5225760492867435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	32043	1.0975671160767226	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	18059	0.6185739334403624	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	14935	0.5115677333147911	No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	12517	0.4287441123469193	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG	11956	0.4095282102116935	No Hit
AGGACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	9361	0.32064181798190555	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	8630	0.29560291519964155	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	8392	0.2874507142937882	No Hit
AGGACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	7285	0.24953270419807522	No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	7017	0.2403529149427445	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6227	0.21329308840650846	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	5934	0.20325697552661331	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	5862	0.20079076348786776	No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5525	0.18924752102873926	No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5332	0.18263670264710186	No Hit
AGGACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	4809	0.16472241242121394	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4482	0.15352169941191118	No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	4315	0.14780145759982077	No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4299	0.14725341048009954	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	4075	0.13958075080400226	No Hit
AGGACTGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3764	0.12892808491442073	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3725	0.12759222006010024	No Hit
AGGACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	3620	0.1239956608369296	No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3450	0.11817266018989148	No Hit
AGGACTGGGCATATATCTCCTAACCTTTGACGACTCTGTATTTCATTTTTGA	3444	0.117967142519996	No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3331	0.11409655973696478	No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3295	0.11286345371759199	No Hit
AGGACTGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC	3169	0.10854758264978728	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	2991	0.10245055844288853	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	2951	0.10108044064358543	No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC	2949	0.10101193475362027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.425294498257724E-4	0.0	0.0	0.0	0.0
8	5.480471197212358E-4	0.0	0.0	0.0	0.0
9	0.0011303471844250489	0.0	0.0	0.0	0.0
10	0.010960942394424716	0.0	0.0	0.0	0.0
11	0.01921590213522583	0.0	0.0	0.0	0.0
12	0.025484191067037466	0.0	0.0	0.0	0.0
13	0.03257455067843096	0.0	0.0	0.0	0.0
14	0.048502170095329374	0.0	0.0	0.0	0.0
15	0.05610632388146152	0.0	0.0	0.0	0.0
16	0.06614243676135664	0.0	0.0	0.0	0.0
17	0.07124612556376066	0.0	0.0	0.0	0.0
18	0.07634981436616467	0.0	0.0	0.0	0.0
19	0.08453626821700062	0.0	0.0	0.0	0.0
20	0.09172938666334185	0.0	0.0	0.0	0.0
21	0.09806618148511864	0.0	0.0	0.0	0.0
22	0.10402619391208708	0.0	0.0	0.0	0.0
23	0.11207563598299272	0.0	0.0	0.0	0.0
24	0.12067312517361961	0.0	0.0	0.0	0.0
25	0.1267358964355358	0.0	0.0	0.0	0.0
26	0.13557315624104072	0.0	0.0	0.0	0.0
27	0.14321156297215543	0.0	0.0	0.0	0.0
28	0.17852634924919258	0.0	0.0	0.0	0.0
29	0.18832269151420966	0.0	0.0	0.0	0.0
30	0.20198961656225797	0.0	0.0	0.0	0.0
31	0.20846342316396507	0.0	0.0	0.0	0.0
32	0.21514274743556763	0.0	0.0	0.0	0.0
33	0.2204862068528497	0.0	0.0	0.0	0.0
34	0.23045381384277966	0.0	0.0	0.0	0.0
35	0.27354401863086186	0.0	0.0	0.0	0.0
36	0.2876562319636837	0.0	0.0	0.0	0.0
37	0.2982746449082826	0.0	0.0	0.0	0.0
38	0.30680362820894436	0.0	0.0	0.0	0.0
39	0.3146132996649719	0.0	0.0	0.0	0.0
40	0.3231422829656337	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	5.6170393E-9	46.0	14
CGTATAG	20	6.313061E-4	46.0	25
TAACGTT	20	6.313061E-4	46.0	30
ATATGCG	20	6.313061E-4	46.0	14
ACGATTT	50	1.6370905E-11	46.0	39
CTAACGT	20	6.313061E-4	46.0	27
ATTTTCG	30	1.862396E-6	46.0	10
CGTACTC	25	3.418458E-5	46.0	13
GTATCGC	20	6.313061E-4	46.0	18
TATACGC	30	1.862396E-6	46.0	32
CGATCAA	40	5.6170393E-9	46.0	10
TATAACG	20	6.313061E-4	46.0	42
CGATAGC	20	6.313061E-4	46.0	41
TAATGCG	20	6.313061E-4	46.0	40
TAATCGA	55	1.8189894E-12	46.0	36
TGGGATA	2720	0.0	43.71691	6
ACTGGGC	36980	0.0	43.064358	4
AGGACTG	316420	0.0	42.255104	1
CTGGGAT	24055	0.0	42.118065	5
GACTGGG	308480	0.0	42.07297	3
>>END_MODULE