FastQCFastQC Report
Fri 17 Jun 2016
SRR1527298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840641
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC300761.0587751144900044No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT181500.6389402955178074No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA144440.5084767839371466No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC123300.43405696108730385No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG120450.4240240142981813No Hit
AGGACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT90810.3196813676913063No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT83640.2944405857691979No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC81120.2855693486082895No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC71710.2524430225431514No Hit
AGGACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC67650.23815047378391005No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG59920.2109383058260442No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT55550.1955544540827229No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG54790.19287900160562352No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC54280.19108363218020158No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG53040.18671842024388158No Hit
AGGACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA46980.1653852070712209No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC43300.15243038455052926No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT41650.14662183640945828No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT41290.1453545168150428No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT39090.1376097859602815No Hit
AGGACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA36330.1278936690697628No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG35900.1263799262208776No Hit
AGGACTGGGCATATATCTCCTAACCTTTGACGACTCTGTATTTCATTTTTGA35010.12324683055690598No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA33070.1164173860758892No Hit
AGGACTGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC32690.11507965983733953No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG32010.11268583393677695No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC30860.10863745189906081No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA29910.10529313630268662No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC28880.10166719412977564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG453.110472E-1046.022
TCGTACG206.313032E-446.015
TTACGAC405.6170393E-946.038
TAATGCG253.4184355E-546.040
ACTGGGC357600.043.079984
AGGACTG3093650.042.0410841
CTGGGAC151400.041.974245
GACTGGG3008750.041.9660643
CTGGGGG668950.041.8878825
ACTGGGA856250.041.745174
GGACTGG3096000.041.7402422
ACTGGGG1661200.041.5625464
CTGGGAT231250.041.544225
TGGGATA26100.041.4176256
TGGGGGG381300.041.313146
TGGGCGA23300.040.669536
ACTGGGT149500.040.6153834
TGGGGGC150400.040.4946826
TGGGATT86750.040.3527376
TGGGACA40350.040.2998776
Produced by FastQC (version 0.11.5)