##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2840641
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35169245251336	33.0	31.0	34.0	31.0	34.0
2	32.69284784666559	34.0	31.0	34.0	31.0	34.0
3	32.99013004459205	34.0	31.0	34.0	31.0	34.0
4	35.97508766507278	37.0	35.0	37.0	35.0	37.0
5	36.35156290428815	37.0	37.0	37.0	35.0	37.0
6	36.34378226604488	37.0	37.0	37.0	35.0	37.0
7	36.52016076653122	37.0	37.0	37.0	35.0	37.0
8	36.63892586215576	37.0	37.0	37.0	35.0	37.0
9	38.644867478854245	39.0	39.0	39.0	39.0	39.0
10	38.126137727365055	39.0	39.0	39.0	37.0	39.0
11	37.85836929059322	39.0	38.0	39.0	35.0	39.0
12	37.32593171752432	39.0	37.0	39.0	35.0	39.0
13	37.137418631921456	39.0	37.0	39.0	34.0	39.0
14	37.90425611684123	40.0	37.0	40.0	34.0	40.0
15	37.974573696570594	40.0	37.0	40.0	34.0	40.0
16	37.90205872547781	40.0	37.0	40.0	34.0	40.0
17	37.874812762330755	40.0	37.0	40.0	33.0	40.0
18	37.87162932591623	40.0	37.0	40.0	33.0	40.0
19	37.727628376834666	40.0	37.0	40.0	33.0	40.0
20	37.76268349291586	40.0	37.0	40.0	33.0	40.0
21	33.59422045939631	39.0	34.0	40.0	10.0	40.0
22	34.34806510220756	39.0	34.0	40.0	17.0	40.0
23	36.371459821920475	39.0	34.0	40.0	30.0	40.0
24	37.17260118402854	39.0	35.0	40.0	32.0	40.0
25	37.438198279895275	39.0	36.0	40.0	33.0	40.0
26	37.49193157459883	40.0	36.0	40.0	33.0	40.0
27	37.483641896318474	40.0	36.0	40.0	34.0	40.0
28	37.34188058258682	40.0	35.0	40.0	33.0	40.0
29	37.34140392960603	40.0	35.0	40.0	33.0	40.0
30	37.22887580655211	40.0	35.0	40.0	33.0	40.0
31	37.08523181915631	39.0	35.0	40.0	32.0	40.0
32	36.95768490280891	39.0	35.0	40.0	32.0	40.0
33	36.634810945839334	39.0	35.0	40.0	31.0	40.0
34	36.422010032242724	39.0	35.0	40.0	30.0	40.0
35	36.4046199431748	39.0	35.0	40.0	30.0	40.0
36	36.376404128504795	39.0	35.0	40.0	31.0	40.0
37	36.32089306603685	38.0	35.0	40.0	31.0	40.0
38	36.29338413407397	38.0	35.0	40.0	31.0	40.0
39	36.137281338965394	38.0	35.0	40.0	30.0	40.0
40	31.219462790264593	37.0	30.0	40.0	8.0	40.0
41	32.12206751926766	36.0	30.0	40.0	13.0	40.0
42	33.96943541968169	36.0	31.0	40.0	23.0	40.0
43	34.8495065022296	37.0	33.0	40.0	27.0	40.0
44	35.584244894022156	38.0	34.0	40.0	30.0	40.0
45	35.85961513616117	38.0	35.0	40.0	30.0	40.0
46	35.87196375747587	38.0	35.0	40.0	31.0	40.0
47	35.82608995645701	37.0	35.0	40.0	31.0	40.0
48	35.689742209592836	37.0	35.0	40.0	30.0	40.0
49	35.5855551616695	37.0	35.0	40.0	30.0	40.0
50	35.44751519111355	37.0	35.0	40.0	30.0	40.0
51	35.29515626930682	36.0	35.0	40.0	30.0	40.0
52	34.88093391597178	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	3.0
14	15.0
15	17.0
16	74.0
17	185.0
18	380.0
19	743.0
20	1412.0
21	2492.0
22	4293.0
23	6665.0
24	9986.0
25	13762.0
26	19089.0
27	25105.0
28	33252.0
29	41852.0
30	54699.0
31	73933.0
32	109772.0
33	124053.0
34	237577.0
35	304992.0
36	339240.0
37	352296.0
38	719652.0
39	365098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.54929186757496	0.347210365547776	0.07153315044034075	0.031964616436923915
8	99.28903370753291	0.5770176520017841	0.05350904954198718	0.08043959092331626
9	97.57199167371027	1.2994954307848123	0.4906639029711956	0.6378489925337274
10	54.540612488519315	28.1682901852082	4.893085750716124	12.398011575556362
11	35.704828593264686	21.02363515840263	19.852913479739257	23.418622768593426
12	31.993342347730668	14.559636363764374	28.614703512341055	24.8323177761639
13	26.665213942909364	18.24947256622713	26.928957231836055	28.156356259027454
14	28.838631844009853	16.0183212169366	31.141175530452458	24.001871408601087
15	33.20201320758237	16.675743256539633	27.72332019428009	22.3989233415979
16	34.47316996410317	19.39400297327258	24.105052345579747	22.027774717044498
17	33.67447699304488	19.217528719750227	21.4878965698235	25.620097717381395
18	33.538204933323144	19.09171204668242	25.742534871530758	21.627548148463674
19	33.46765747590069	21.157055749036925	23.325509981725958	22.049776793336434
20	33.83669390113006	23.245246407412974	21.43604207641867	21.482017615038295
21	38.261821891608264	19.341937259935346	23.333184305936584	19.063056542519803
22	31.782967295057702	18.41327362380533	25.576762427916798	24.22699665322017
23	32.3178113672231	20.73260929487394	23.42034773137471	23.529231606528246
24	32.80815844029569	20.307388367625475	22.3744218294392	24.51003136263963
25	28.113161782851126	18.725562293862545	21.900866740992615	31.260409182293714
26	27.695439163202952	19.03992795992172	25.36071964039102	27.9039132364843
27	26.14012119095655	18.087290861464016	24.074636675313776	31.697951272265662
28	25.80495036155572	24.66186329071502	21.827186187906182	27.70600015982308
29	32.12943839084207	21.179691485126067	21.39369247997195	25.297177644059914
30	29.834181792067355	20.35889082780964	24.406005545931357	25.40092183419165
31	34.54428067467871	18.80821969407609	22.34256282296848	24.30493680827672
32	36.105900041575126	17.958622719308774	23.63660173883289	22.29887550028321
33	36.17496895947077	18.08155271996708	23.85482713232682	21.88865118823533
34	29.118885490986013	20.91735632908206	27.615985265297518	22.347772914634408
35	26.842392262873062	27.387269281827585	25.85300289617731	19.917335559122044
36	29.8431234358724	28.002095301729433	20.946434273109485	21.208346989288685
37	31.300681782738472	27.166966892331697	19.709143112417234	21.823208212512597
38	31.743293151088082	25.943968280398682	19.54499002161836	22.76774854689487
39	30.65649619223267	23.133405453205807	19.469654912394773	26.74044344216675
40	28.509762409259036	27.564658821723686	19.18060043490184	24.744978334115434
41	24.429908601614915	24.337887117731526	20.82889038072745	30.40331389992611
42	25.057443020782987	21.52486005799395	22.570222706776395	30.84747421444667
43	26.046867590800808	19.147368498870502	24.98763483312393	29.81812907720476
44	26.77501310443664	20.398635378423393	23.136573752191847	29.689777764948122
45	27.915143096223705	20.28140831593996	22.0934641160217	29.70998447181464
46	25.613021849645907	22.215197203729723	24.59793405784117	27.573846888783198
47	27.569692896779287	20.18160689787974	25.44239134758669	26.806308857754292
48	27.894549152814452	20.283872548484656	26.120512940565177	25.701065358135715
49	29.503376174602845	19.543863515312214	24.5867746047459	26.36598570533904
50	25.918058635357298	20.314534642005096	25.27862549333055	28.488781229307047
51	24.421847040861554	21.773993968262797	26.24087309871258	27.56328589216307
52	25.090146906983318	21.020748485993128	25.669628791529796	28.219475815493755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.0
6	8.0
7	6.5
8	5.0
9	39.5
10	74.0
11	262.5
12	451.0
13	775.0
14	1691.5
15	2284.0
16	2503.0
17	2722.0
18	3083.0
19	3444.0
20	3691.0
21	3938.0
22	4156.5
23	4375.0
24	3939.5
25	3504.0
26	5445.5
27	7387.0
28	8962.5
29	10538.0
30	11188.0
31	11838.0
32	14508.5
33	17179.0
34	19374.5
35	21570.0
36	24499.0
37	27428.0
38	33890.0
39	38088.5
40	35825.0
41	45093.5
42	54362.0
43	55961.5
44	57561.0
45	68065.0
46	78569.0
47	85708.5
48	92848.0
49	107095.0
50	121342.0
51	122103.5
52	122865.0
53	133507.5
54	144150.0
55	174077.5
56	204005.0
57	234879.5
58	265754.0
59	285551.5
60	305349.0
61	293980.5
62	282612.0
63	296865.5
64	263209.5
65	215300.0
66	177465.5
67	139631.0
68	113059.5
69	86488.0
70	73555.5
71	60623.0
72	52623.5
73	44624.0
74	38745.0
75	32866.0
76	24598.5
77	16331.0
78	11167.0
79	6003.0
80	4660.5
81	3318.0
82	1952.5
83	587.0
84	403.5
85	220.0
86	134.5
87	49.0
88	36.0
89	16.0
90	9.0
91	9.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2840641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.410394370926713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.04884252319499	5.891383908470109
2	10.283602744998122	1.7297830927875741
3	4.397262093130214	1.1094812506665532
4	2.2164354890713907	0.7456438624323287
5	1.2698776325370271	0.5340085846227577
6	0.8106203857376383	0.4090582277499764
7	0.5045296784222043	0.29703054971573
8	0.35415708077518104	0.2382880574860336
9	0.28416302404093446	0.21509307880374487
>10	4.018398305913119	9.117925878047766
>50	2.910937186689794	17.825396367231193
>100	2.707371731876814	40.07186382830826
>500	0.1352221366263753	7.765511485873178
>1k	0.051257488612885954	7.714642194016855
>5k	0.004881665582179614	2.7611063330343875
>10k+	0.002440832791089807	3.5737833007535693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	30076	1.0587751144900044	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	18150	0.6389402955178074	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	14444	0.5084767839371466	No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	12330	0.43405696108730385	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG	12045	0.4240240142981813	No Hit
AGGACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	9081	0.3196813676913063	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	8364	0.2944405857691979	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	8112	0.2855693486082895	No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	7171	0.2524430225431514	No Hit
AGGACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	6765	0.23815047378391005	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5992	0.2109383058260442	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	5555	0.1955544540827229	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	5479	0.19287900160562352	No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5428	0.19108363218020158	No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5304	0.18671842024388158	No Hit
AGGACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	4698	0.1653852070712209	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4330	0.15243038455052926	No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4165	0.14662183640945828	No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	4129	0.1453545168150428	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3909	0.1376097859602815	No Hit
AGGACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	3633	0.1278936690697628	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3590	0.1263799262208776	No Hit
AGGACTGGGCATATATCTCCTAACCTTTGACGACTCTGTATTTCATTTTTGA	3501	0.12324683055690598	No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3307	0.1164173860758892	No Hit
AGGACTGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC	3269	0.11507965983733953	No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3201	0.11268583393677695	No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3086	0.10863745189906081	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	2991	0.10529313630268662	No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC	2888	0.10166719412977564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.2243986480516193E-4	0.0	0.0	0.0	0.0
8	8.096764075432271E-4	0.0	0.0	0.0	0.0
9	0.0013025229164825827	0.0	0.0	0.0	0.0
10	0.010807419874598725	0.0	0.0	0.0	0.0
11	0.01865776069556132	0.0	0.0	0.0	0.0
12	0.025804035075181975	0.0	0.0	0.0	0.0
13	0.033936002472681345	0.0	0.0	0.0	0.0
14	0.048862211029130395	0.0	0.0	0.0	0.0
15	0.05692377178249557	0.0	0.0	0.0	0.0
16	0.06470370595932397	0.0	0.0	0.0	0.0
17	0.06924493450597946	0.0	0.0	0.0	0.0
18	0.07396217966297043	0.0	0.0	0.0	0.0
19	0.07959471119370592	0.0	0.0	0.0	3.520332206709683E-5
20	0.08624813906438723	0.0	0.0	0.0	3.520332206709683E-5
21	0.09177506062892143	0.0	0.0	0.0	3.520332206709683E-5
22	0.09726677887138853	0.0	0.0	0.0	3.520332206709683E-5
23	0.10557476287922339	0.0	0.0	0.0	3.520332206709683E-5
24	0.11338990037811889	0.0	0.0	0.0	3.520332206709683E-5
25	0.11969129502812921	0.0	0.0	0.0	3.520332206709683E-5
26	0.128703345477306	0.0	0.0	0.0	3.520332206709683E-5
27	0.13613124643346344	0.0	0.0	0.0	3.520332206709683E-5
28	0.1709473319578222	0.0	0.0	0.0	3.520332206709683E-5
29	0.18013539901733447	0.0	0.0	0.0	3.520332206709683E-5
30	0.19333664479249577	0.0	0.0	0.0	3.520332206709683E-5
31	0.20037730920591515	0.0	0.0	0.0	3.520332206709683E-5
32	0.20773480351793838	0.0	0.0	0.0	3.520332206709683E-5
33	0.21248725199699645	0.0	0.0	0.0	3.520332206709683E-5
34	0.2219921489551126	0.0	0.0	0.0	3.520332206709683E-5
35	0.2607862098730533	0.0	0.0	0.0	3.520332206709683E-5
36	0.2740578622923488	0.0	0.0	0.0	3.520332206709683E-5
37	0.2830699127415256	0.0	0.0	0.0	3.520332206709683E-5
38	0.2907442369521527	0.0	0.0	0.0	3.520332206709683E-5
39	0.2978553080097063	0.0	0.0	0.0	3.520332206709683E-5
40	0.3075362215781579	0.0	0.0	0.0	3.520332206709683E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	45	3.110472E-10	46.0	22
TCGTACG	20	6.313032E-4	46.0	15
TTACGAC	40	5.6170393E-9	46.0	38
TAATGCG	25	3.4184355E-5	46.0	40
ACTGGGC	35760	0.0	43.07998	4
AGGACTG	309365	0.0	42.041084	1
CTGGGAC	15140	0.0	41.97424	5
GACTGGG	300875	0.0	41.966064	3
CTGGGGG	66895	0.0	41.887882	5
ACTGGGA	85625	0.0	41.74517	4
GGACTGG	309600	0.0	41.740242	2
ACTGGGG	166120	0.0	41.562546	4
CTGGGAT	23125	0.0	41.54422	5
TGGGATA	2610	0.0	41.417625	6
TGGGGGG	38130	0.0	41.31314	6
TGGGCGA	2330	0.0	40.66953	6
ACTGGGT	14950	0.0	40.615383	4
TGGGGGC	15040	0.0	40.494682	6
TGGGATT	8675	0.0	40.352737	6
TGGGACA	4035	0.0	40.299877	6
>>END_MODULE