SRR1527297_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451679
Sequences flagged as poor quality	0
Sequence length	50
%GC	34

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43371	9.602173224790171	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGA	16477	3.6479446686695645	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15995	3.541231715443933	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15063	3.3348904863852424	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4155	0.9199010801919061	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3584	0.7934838679681808	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2848	0.6305362879390009	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	1810	0.40072706501741284	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1694	0.3750451094693355	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	1465	0.32434538687873465	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1422	0.3148253516324646	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1318	0.29180015010660226	No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAA	1240	0.27453124896220543	No Hit
CAAAGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1097	0.24287159686414467	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAA	1072	0.23733669265119695	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGAGGGA	998	0.22095337618087182	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAA	962	0.21298311411422716	No Hit
CAAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	954	0.2112119447660839	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	943	0.2087765869123869	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGG	897	0.19859236316056317	No Hit
CAAAGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	822	0.18198765052172008	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	807	0.17866670799395146	Illumina Single End Adapter 2 (100% over 23bp)
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAA	791	0.17512436929766492	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG	790	0.17490297312914704	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGG	718	0.15896244899585768	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	713	0.15785546815326815	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGCGGGA	703	0.15564150646808905	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAGTGCAGTGCTCAAAGTGG	701	0.15519871413105324	No Hit
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAGAGTGCAGTGCTCAAAGT	698	0.15453452562549955	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	691	0.15298475244587417	Illumina Single End Adapter 2 (95% over 22bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA	671	0.148556829075516	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAA	623	0.13792981298665644	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	584	0.12929536241445805	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAA	576	0.1275241930663148	No Hit
CAAAGTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	551	0.12198928885336711	No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	541	0.11977532716818803	Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	525	0.1162329884719015	No Hit
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	505	0.11180506510154337	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGCAAAAAAAAAAAAAAAAAAAAA	471	0.1042775953719345	No Hit
CAAAGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	461	0.10206363368675542	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	461	0.10206363368675542	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAA	460	0.10184223751823751	No Hit
CAAAGTGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	453	0.10029246433861216	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGAAC	20	7.8514E-4	44.000004	39
CGTTCAT	20	7.8514E-4	44.000004	33
CGACAAT	40	8.292773E-9	44.000004	30
GGGTCGC	40	8.292773E-9	44.000004	7
GGCACCG	20	7.8514E-4	44.000004	37
ATAAGTC	40	8.292773E-9	44.000004	22
AGGATTA	20	7.8514E-4	44.000004	42
TTCAAGC	40	8.292773E-9	44.000004	10
GTCGCGA	40	8.292773E-9	44.000004	9
TAAGTCC	40	8.292773E-9	44.000004	23
TCGGCGG	20	7.8514E-4	44.000004	24
GAAGCAC	20	7.8514E-4	44.000004	9
CCGCCAG	20	7.8514E-4	44.000004	18
TTATTGC	40	8.292773E-9	44.000004	30
TCGAACT	40	8.292773E-9	44.000004	40
TCGCGAA	40	8.292773E-9	44.000004	10
CGAAGTT	40	8.292773E-9	44.000004	13
CGCCAGC	20	7.8514E-4	44.000004	19
GCGAAGT	40	8.292773E-9	44.000004	12
ACCGCCA	20	7.8514E-4	44.000004	40

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content