##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451679
Sequences flagged as poor quality	0
Sequence length	50
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29349161683408	31.0	31.0	33.0	28.0	33.0
2	31.458378627299474	31.0	31.0	34.0	28.0	34.0
3	32.3458318850334	34.0	31.0	34.0	30.0	34.0
4	36.10521631512645	37.0	35.0	37.0	35.0	37.0
5	36.07747316124947	37.0	35.0	37.0	35.0	37.0
6	33.43005762942266	35.0	33.0	35.0	30.0	36.0
7	35.334396772929445	35.0	35.0	36.0	35.0	37.0
8	36.334343637849	37.0	35.0	37.0	35.0	37.0
9	38.49192014682994	39.0	39.0	39.0	37.0	39.0
10	37.599164450860016	39.0	37.0	39.0	35.0	39.0
11	37.544621290783944	39.0	37.0	39.0	35.0	39.0
12	37.24746778132258	39.0	37.0	39.0	34.0	39.0
13	36.91935423165567	39.0	37.0	39.0	33.0	39.0
14	37.96398769922888	40.0	38.0	40.0	33.0	40.0
15	37.95509421513951	40.0	38.0	40.0	33.0	40.0
16	37.29056475948627	40.0	37.0	40.0	32.0	40.0
17	37.827251211590536	40.0	38.0	40.0	33.0	40.0
18	37.945421416536966	40.0	38.0	40.0	33.0	40.0
19	37.22132532174398	40.0	37.0	40.0	32.0	40.0
20	37.61867830915318	40.0	37.0	40.0	32.0	40.0
21	37.88533892432458	40.0	38.0	40.0	33.0	40.0
22	37.77936986222517	40.0	38.0	40.0	33.0	40.0
23	37.84560495396067	40.0	38.0	40.0	33.0	40.0
24	37.59809732132776	39.0	37.0	40.0	33.0	40.0
25	36.55271996262832	37.0	36.0	40.0	32.0	40.0
26	35.666451174395974	37.0	35.0	39.0	31.0	40.0
27	35.21407016930165	35.0	35.0	39.0	30.0	40.0
28	35.05108273796214	35.0	35.0	39.0	30.0	40.0
29	35.03054160144705	35.0	35.0	40.0	30.0	40.0
30	34.50052581590023	35.0	35.0	39.0	26.0	40.0
31	34.216273061178406	35.0	35.0	39.0	25.0	40.0
32	32.6231416559105	35.0	33.0	38.0	16.0	40.0
33	32.710354034613076	35.0	33.0	38.0	18.0	40.0
34	32.324216977100996	35.0	33.0	39.0	16.0	40.0
35	32.52090311925062	35.0	33.0	39.0	17.0	40.0
36	31.786534242238403	35.0	31.0	38.0	12.0	40.0
37	30.74064988631307	35.0	27.0	38.0	10.0	40.0
38	30.223424157421533	35.0	25.0	38.0	9.0	40.0
39	29.727921820584974	35.0	24.0	38.0	9.0	40.0
40	29.10717345725615	35.0	20.0	38.0	8.0	40.0
41	28.640917554280804	35.0	18.0	37.0	8.0	40.0
42	28.26692407661193	35.0	17.0	37.0	8.0	40.0
43	28.257824694085844	35.0	17.0	36.0	8.0	40.0
44	27.86227829941175	35.0	15.0	35.0	8.0	40.0
45	27.43148342074792	35.0	15.0	35.0	8.0	40.0
46	26.952612364090427	34.0	14.0	35.0	8.0	40.0
47	26.906236508670982	34.0	13.0	35.0	8.0	39.0
48	26.92201319963957	34.0	13.0	35.0	8.0	39.0
49	26.688781191952692	34.0	12.0	35.0	8.0	39.0
50	26.047011262423094	33.0	10.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	5.0
15	10.0
16	42.0
17	113.0
18	249.0
19	450.0
20	943.0
21	1610.0
22	2583.0
23	3924.0
24	6043.0
25	9052.0
26	12812.0
27	18064.0
28	24322.0
29	28140.0
30	26416.0
31	21090.0
32	19091.0
33	19708.0
34	25815.0
35	36128.0
36	98279.0
37	48840.0
38	44311.0
39	3638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.46887059172555	0.21873941449569273	0.12575302371817154	0.18663697006059612
8	98.18654398366982	0.9767998955010084	0.3688460167508341	0.46781010407833884
9	94.9284779677603	3.0213935117638853	0.6772508794962795	1.3728776409795453
10	34.10320160999294	46.860048839994775	10.89756220678845	8.139187343223838
11	17.89058158559508	65.64462815406516	9.286905080820672	7.177885179519084
12	34.39500176009954	52.54970897473649	6.460118801184027	6.595170463979951
13	14.597534975059723	54.885438552600405	7.779418569382238	22.73760790295763
14	15.368215037670558	71.52601737074338	6.49997011151725	6.60579748006881
15	28.21893424312399	56.026957197478744	7.555365646842116	8.198742912555156
16	13.532840800657103	57.26611155267347	23.552788595440568	5.648259051228859
17	30.000952003524628	55.300113576234445	7.078256903686025	7.620677516554898
18	25.72069987756792	56.690260118358395	9.158716699248803	8.430323304824887
19	13.936224619696732	56.28709769548729	22.200500798133188	7.576176886682799
20	11.043240885673232	67.53513003703958	9.70180150062323	11.719827576663958
21	11.00427516001408	56.09913234841558	23.61389393795151	9.282698553618832
22	10.698305655122333	57.997825889625155	7.358765849198214	23.9451026060543
23	10.351599255223288	73.87702328423504	7.239433314367062	8.531944146174606
24	11.145083123191469	72.30356071457827	8.51268267951355	8.038673482716709
25	11.65938642265857	60.08382058940088	8.603012316268854	19.653780671671697
26	27.133650225049205	57.81982337013676	8.633564987524325	6.412961417289713
27	14.965716803305002	57.7684594590406	9.615456994901246	17.65036674275315
28	18.450935288113904	68.28743421766343	7.284598132744715	5.977032361477952
29	29.87652735681756	55.946147595969705	8.050407479648158	6.1269175675645755
30	18.711297182290963	67.36310521410115	8.127010553955353	5.798587049652518
31	28.826666725705646	56.133448754535856	9.282477157450312	5.7574073623081885
32	16.95961069697728	56.54635260882175	19.357552598194737	7.136484096006235
33	25.9219489947507	57.59178531656332	8.09247275166656	8.393792937019432
34	14.773766325199977	59.156170643310844	9.204324309963493	16.865738721525688
35	13.78921756380084	70.37210054042805	8.662125093263136	7.176556802507976
36	23.998016290330078	59.15838460499603	9.47221367387016	7.371385430803734
37	15.240690844604243	59.019126414998254	17.42277148151674	8.317411258880753
38	25.236506457019253	56.763542250137824	9.079014078582356	8.92093721426057
39	16.621095955313397	54.989716147972345	9.645566873819682	18.743621022894576
40	17.68534733737898	53.70561837056847	19.15608208484344	9.452952207209101
41	17.924233803209802	53.66798102192043	10.300235344127135	18.107549830742627
42	18.018105778661393	62.35822342858535	10.212562461394043	9.411108331359218
43	17.547417524392323	63.83648564577942	9.704015462308409	8.912081367519855
44	17.85316563311555	63.70984703738717	10.110498827707287	8.326488501789989
45	25.188463488450868	56.016551577558396	9.597745301419813	9.19723963257092
46	18.358613085841938	55.70216901826297	16.642571383659636	9.29664651223546
47	24.867439044099903	54.9186479778781	10.020390587120499	10.193522390901503
48	25.354067822502262	55.1500069739793	9.947108455341072	9.548816748177357
49	25.406981506778042	54.80684291277655	9.651323174201147	10.134852406244258
50	17.804015683704577	61.27869571089203	10.745905831353683	10.171382774049713
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.0
4	12.0
5	227.0
6	442.0
7	1105.5
8	1769.0
9	31683.0
10	61597.0
11	46881.5
12	32166.0
13	22366.0
14	12566.0
15	9322.5
16	6079.0
17	6086.0
18	6093.0
19	6329.0
20	6565.0
21	7499.0
22	8433.0
23	9978.5
24	11524.0
25	13117.0
26	14710.0
27	14989.0
28	15268.0
29	15230.5
30	15193.0
31	16194.5
32	17196.0
33	17054.5
34	16913.0
35	15944.0
36	14975.0
37	14874.5
38	14774.0
39	13518.5
40	12263.0
41	11045.0
42	9827.0
43	10015.5
44	10204.0
45	11128.0
46	12052.0
47	11206.0
48	10360.0
49	12576.0
50	14792.0
51	29644.0
52	44496.0
53	40530.5
54	36565.0
55	29584.5
56	22604.0
57	17201.5
58	11799.0
59	8592.5
60	5386.0
61	3829.5
62	2273.0
63	1570.5
64	868.0
65	779.0
66	690.0
67	559.5
68	429.0
69	371.5
70	314.0
71	261.5
72	209.0
73	194.0
74	179.0
75	117.0
76	55.0
77	38.5
78	22.0
79	14.5
80	7.0
81	6.5
82	6.0
83	4.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	451679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.73932496788899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20526969884232	26.586057137754675
2	8.418340572123132	5.512215753622074
3	3.2184572185887275	3.1611035032387345
4	1.7724583334605537	2.321163574848321
5	1.1278584047824856	1.8462661415969337
6	0.7486512555305779	1.4706202045460213
7	0.5549545149944987	1.271818534616148
8	0.3919328949348858	1.0265294730302976
9	0.33093285658706495	0.975106650092015
>10	1.860789147823183	11.373690855723517
>50	0.17721664709747084	4.132078686381506
>100	0.1668328591816034	11.001954132936804
>500	0.015921808137663394	3.7993729211550877
>1k	0.00761477780496945	4.919195254931471
>5k	0.0	0.0
>10k+	0.002769010110897982	20.602827175526393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43371	9.602173224790171	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGA	16477	3.6479446686695645	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15995	3.541231715443933	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15063	3.3348904863852424	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4155	0.9199010801919061	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3584	0.7934838679681808	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2848	0.6305362879390009	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	1810	0.40072706501741284	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1694	0.3750451094693355	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	1465	0.32434538687873465	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1422	0.3148253516324646	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1318	0.29180015010660226	No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAA	1240	0.27453124896220543	No Hit
CAAAGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1097	0.24287159686414467	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAA	1072	0.23733669265119695	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGAGGGA	998	0.22095337618087182	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAA	962	0.21298311411422716	No Hit
CAAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	954	0.2112119447660839	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	943	0.2087765869123869	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGG	897	0.19859236316056317	No Hit
CAAAGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	822	0.18198765052172008	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	807	0.17866670799395146	Illumina Single End Adapter 2 (100% over 23bp)
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAA	791	0.17512436929766492	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG	790	0.17490297312914704	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGG	718	0.15896244899585768	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	713	0.15785546815326815	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGCGGGA	703	0.15564150646808905	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAGTGCAGTGCTCAAAGTGG	701	0.15519871413105324	No Hit
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAGAGTGCAGTGCTCAAAGT	698	0.15453452562549955	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	691	0.15298475244587417	Illumina Single End Adapter 2 (95% over 22bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA	671	0.148556829075516	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAA	623	0.13792981298665644	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	584	0.12929536241445805	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAA	576	0.1275241930663148	No Hit
CAAAGTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	551	0.12198928885336711	No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	541	0.11977532716818803	Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	525	0.1162329884719015	No Hit
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	505	0.11180506510154337	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGCAAAAAAAAAAAAAAAAAAAAA	471	0.1042775953719345	No Hit
CAAAGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	461	0.10206363368675542	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	461	0.10206363368675542	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAA	460	0.10184223751823751	No Hit
CAAAGTGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	453	0.10029246433861216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005313508044429783	0.0	0.0	0.0	0.0
8	0.03276663294065033	0.0	0.0	0.0	0.0
9	0.05047832642208294	0.0	0.0	0.0	0.0
10	0.29755645048806784	0.0	0.0	0.0	0.0
11	0.5661100029002898	0.0	0.0	0.0	0.0
12	0.779093117014517	0.0	0.0	0.0	0.0
13	0.9001968211938124	0.0	0.0	0.0	0.0
14	1.0582736855155985	0.0	0.0	0.0	0.0
15	1.1767206356726791	0.0	0.0	0.0	0.0
16	1.294503397324206	0.0	0.0	0.0	0.0
17	1.3843902417424765	0.0	0.0	0.0	0.0
18	1.4857896869236782	0.0	0.0	0.0	0.0
19	1.6031296562381692	0.0	0.0	0.0	0.0
20	1.7127207596545335	0.0	0.0	0.0	0.0
21	1.8185481282060934	0.0	0.0	0.0	0.0
22	1.9445225480927828	0.0	0.0	0.0	0.0
23	2.092193792494227	0.0	0.0	0.0	0.0
24	2.2546985801863713	0.0	0.0	0.0	0.0
25	2.4030340130933694	0.0	0.0	0.0	0.0
26	2.5712951011669793	0.0	0.0	0.0	0.0
27	2.737120831386892	0.0	0.0	0.0	0.0
28	3.0789565155785414	0.0	0.0	0.0	0.0
29	3.21666493239668	0.0	0.0	0.0	0.0
30	3.38913254767213	0.0	0.0	0.0	0.0
31	3.4880966349996347	0.0	0.0	0.0	0.0
32	3.585732345316032	0.0	0.0	0.0	0.0
33	3.6758405859028205	0.0	0.0	0.0	0.0
34	3.792073574374722	0.0	0.0	0.0	0.0
35	4.2373012692642344	0.0	0.0	0.0	0.0
36	4.330287660041756	0.0	0.0	0.0	0.0
37	4.407554922854505	0.0	0.0	0.0	0.0
38	4.487700335857988	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGAAC	20	7.8514E-4	44.000004	39
CGTTCAT	20	7.8514E-4	44.000004	33
CGACAAT	40	8.292773E-9	44.000004	30
GGGTCGC	40	8.292773E-9	44.000004	7
GGCACCG	20	7.8514E-4	44.000004	37
ATAAGTC	40	8.292773E-9	44.000004	22
AGGATTA	20	7.8514E-4	44.000004	42
TTCAAGC	40	8.292773E-9	44.000004	10
GTCGCGA	40	8.292773E-9	44.000004	9
TAAGTCC	40	8.292773E-9	44.000004	23
TCGGCGG	20	7.8514E-4	44.000004	24
GAAGCAC	20	7.8514E-4	44.000004	9
CCGCCAG	20	7.8514E-4	44.000004	18
TTATTGC	40	8.292773E-9	44.000004	30
TCGAACT	40	8.292773E-9	44.000004	40
TCGCGAA	40	8.292773E-9	44.000004	10
CGAAGTT	40	8.292773E-9	44.000004	13
CGCCAGC	20	7.8514E-4	44.000004	19
GCGAAGT	40	8.292773E-9	44.000004	12
ACCGCCA	20	7.8514E-4	44.000004	40
>>END_MODULE