SRR1527296_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489202
Sequences flagged as poor quality	0
Sequence length	52
%GC	34

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43343	8.859939247999804	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15962	3.2628648288437088	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	15678	3.204811100526981	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14593	2.9830213286127205	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4048	0.8274700430497014	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3540	0.7236274585958357	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2879	0.5885094500840145	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	1759	0.3595651693983262	No Hit
CAAAGTGGGAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1443	0.2949701759191499	No Hit
CAAAGTGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	1406	0.28740683807506917	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	1379	0.2818876455942535	No Hit
CAAAGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1332	0.27228016238690766	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1275	0.26062853381629675	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA	1124	0.22976193883099413	No Hit
CAAAGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.22710454985874956	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1086	0.22199418645058688	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	976	0.1995085874546711	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGAGGGAAG	974	0.19909975838201804	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGGAG	966	0.19746444209140598	No Hit
CAAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	922	0.18847020249303967	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG	880	0.17988479196732637	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	861	0.17600091577712274	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	811	0.16578018896079738	Illumina Single End Adapter 2 (100% over 25bp)
CAAAGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	807	0.16496253081549136	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	792	0.16189631277059374	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA	744	0.15208441502692138	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGGAG	743	0.15188000049059489	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG	651	0.1330738631485562	No Hit
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAGAGTGCAGTGCTCAAAGTGG	615	0.12571493984080195	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAA	605	0.12367079447753689	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT	600	0.12264872179590434	Illumina Single End Adapter 2 (95% over 24bp)
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	596	0.12183106365059831	No Hit
CAAAGTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.11733394385141517	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	570	0.11651628570610914	No Hit
CAAAGTGGGGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	537	0.1097706060073344	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAA	533	0.10895294786202837	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGCGGGAAG	514	0.10506907167182472	No Hit
CAAAGTGGGAAGCAGGGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	513	0.10486465713549822	No Hit
CAAAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	500	0.10220726816325362	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGTA	55	1.8189894E-12	46.000004	16
TTCATCG	35	1.01796104E-7	46.000004	35
CCAACAG	55	1.8189894E-12	46.000004	24
TTACTGG	55	1.8189894E-12	46.000004	32
AGGATTA	35	1.01796104E-7	46.000004	42
TTATCGA	55	1.8189894E-12	46.000004	44
TTACACC	35	1.01796104E-7	46.000004	46
GGATTAC	35	1.01796104E-7	46.000004	43
CAACAGG	55	1.8189894E-12	46.000004	25
CTGACGC	35	1.01796104E-7	46.000004	20
GCAATGT	55	1.8189894E-12	46.000004	15
GATTGCG	35	1.01796104E-7	46.000004	40
GCGTGCG	35	1.01796104E-7	46.000004	22
ATTACAC	35	1.01796104E-7	46.000004	45
GGTTACT	55	1.8189894E-12	46.000004	30
TCTCGTT	35	1.01796104E-7	46.000004	44
ATTGCGC	35	1.01796104E-7	46.000004	41
CGGGAGT	35	1.01796104E-7	46.000004	17
GTCTCGT	35	1.01796104E-7	46.000004	43
TCGTTGC	35	1.01796104E-7	46.000004	46

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content