##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489202
Sequences flagged as poor quality	0
Sequence length	52
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12624641763525	31.0	31.0	33.0	28.0	33.0
2	31.119331073871326	31.0	31.0	34.0	28.0	34.0
3	32.12271413444753	33.0	31.0	34.0	30.0	34.0
4	35.945445848545184	37.0	35.0	37.0	35.0	37.0
5	36.003021246846906	37.0	35.0	37.0	35.0	37.0
6	33.10272852523089	35.0	33.0	35.0	28.0	35.0
7	35.275434278682425	35.0	35.0	36.0	35.0	37.0
8	36.27438563211107	37.0	35.0	37.0	35.0	37.0
9	38.44840168274046	39.0	39.0	39.0	37.0	39.0
10	37.38270898320121	39.0	37.0	39.0	34.0	39.0
11	37.38750045993271	39.0	37.0	39.0	34.0	39.0
12	37.152658819874	39.0	37.0	39.0	34.0	39.0
13	36.91005760401634	39.0	37.0	39.0	33.0	39.0
14	37.93132693652111	40.0	38.0	40.0	33.0	40.0
15	37.87223069406912	40.0	38.0	40.0	33.0	40.0
16	32.49172938786023	39.0	32.0	40.0	8.0	40.0
17	34.6545394336082	39.0	34.0	40.0	17.0	40.0
18	36.7003528194897	40.0	34.0	40.0	31.0	40.0
19	36.70993577295268	39.0	36.0	40.0	31.0	40.0
20	37.6084480439573	39.0	37.0	40.0	32.0	40.0
21	37.935073854971975	40.0	38.0	40.0	33.0	40.0
22	38.048063989926455	40.0	38.0	40.0	33.0	40.0
23	38.039439740638834	40.0	38.0	40.0	33.0	40.0
24	37.70117456592573	39.0	38.0	40.0	33.0	40.0
25	36.819961897130426	38.0	36.0	40.0	32.0	40.0
26	35.88566890568722	37.0	35.0	40.0	31.0	40.0
27	35.43539682993937	35.0	35.0	40.0	30.0	40.0
28	35.369550819497874	35.0	35.0	40.0	30.0	40.0
29	34.993487352872634	35.0	35.0	39.0	30.0	40.0
30	34.362424928761534	35.0	34.0	39.0	25.0	40.0
31	34.035502716669185	35.0	34.0	39.0	25.0	40.0
32	33.0141005147158	35.0	33.0	38.0	20.0	40.0
33	32.69171426118454	35.0	33.0	38.0	19.0	40.0
34	32.23662004652475	35.0	33.0	38.0	16.0	40.0
35	32.37666444536204	35.0	33.0	38.0	16.0	40.0
36	31.811051467491957	35.0	31.0	38.0	15.0	40.0
37	30.474039353886532	35.0	25.0	38.0	10.0	40.0
38	30.233063233592667	35.0	25.0	38.0	10.0	40.0
39	29.386124750103228	35.0	23.0	38.0	9.0	40.0
40	28.848447062767526	35.0	19.0	37.0	8.0	40.0
41	28.410028985981253	35.0	18.0	37.0	8.0	40.0
42	28.13390174202068	35.0	16.0	36.0	8.0	40.0
43	28.26303449290886	35.0	17.0	36.0	8.0	40.0
44	27.877843917236643	35.0	15.0	35.0	8.0	40.0
45	27.482242509229316	35.0	15.0	35.0	8.0	40.0
46	27.06420865000552	34.0	14.0	35.0	8.0	40.0
47	26.873275252349746	34.0	13.0	35.0	8.0	40.0
48	26.9991373706567	34.0	13.0	35.0	8.0	39.0
49	26.93533346143311	34.0	13.0	35.0	8.0	39.0
50	26.057926991304207	33.0	10.0	35.0	8.0	39.0
51	26.05852592589564	33.0	10.0	35.0	8.0	39.0
52	25.562822719449226	33.0	10.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	47.0
17	117.0
18	316.0
19	588.0
20	1107.0
21	2039.0
22	3541.0
23	5900.0
24	9198.0
25	13398.0
26	19250.0
27	25734.0
28	29517.0
29	27898.0
30	22770.0
31	19705.0
32	19993.0
33	23931.0
34	32656.0
35	51598.0
36	96363.0
37	49769.0
38	31313.0
39	2441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.56500586669719	0.17947596289467335	0.0932130285648873	0.16230514184324676
8	98.30213286127203	0.8959489127190813	0.35097975887261296	0.450938467136275
9	94.80194275575325	3.1308130383767847	0.7078875392986946	1.3593566665712733
10	33.93526600463612	47.3256446212403	10.564756480962874	8.174332893160699
11	18.041218147104875	65.31964301045376	9.272652196843021	7.366486645598341
12	35.43771284663595	51.01880204905132	6.465427369471098	7.07805773484164
13	14.967641178899513	53.38367381981267	7.8219222325338	23.826762768754012
14	15.624833913189235	70.87563010780823	6.572949415578841	6.926586563423697
15	29.18201479143585	54.73546714853986	7.806386727772985	8.2761313322513
16	14.883831219005646	58.055976876627646	21.66998499597303	5.39020690839367
17	31.040347341180123	54.025739878414235	7.209905110772237	7.724007669633402
18	26.78361903671694	55.14450063572921	9.535733705095236	8.536146622458617
19	14.403661473174681	54.71809191295212	23.165072914665107	7.713173699208098
20	11.23564498918647	66.79735569355807	9.953761431882944	12.013237885372504
21	11.281229430787281	54.53146144128601	24.259712756693556	9.92759637123315
22	10.539000249385735	57.15553084410938	7.447843631056291	24.857625275448587
23	10.811689240845295	72.99602209312309	7.514891598971387	8.677397067060234
24	11.29778700822973	71.4214169198	8.855237713664293	8.425558358305976
25	12.168797347516977	58.55086446907413	8.65879534425452	20.621542839154376
26	27.840442189524982	56.273277705324176	8.914109100126328	6.972171005024509
27	15.076389712225216	56.431494556440896	9.94170097423968	18.550414757094206
28	18.774453088908057	67.50013286944862	7.344818704747732	6.380595336895597
29	30.738835900098525	54.882441200158624	8.172697576870085	6.20602532287276
30	19.05123037109415	66.73071655471564	8.310677388890479	5.9073756852997334
31	29.711039611448847	54.85586731043618	9.516109909607891	5.9169831685070795
32	17.493591604286163	55.89879027477402	19.838226335951205	6.769391784988614
33	27.239463452725047	55.61772028732507	8.588476743758203	8.554339516191675
34	15.387099807441507	57.75180804657381	9.24178560185772	17.619306544126967
35	14.32107800049877	69.56124463922878	8.831116798377767	7.286560561894677
36	24.88256384888042	58.21950850568886	9.542479384794012	7.3554482606367095
37	15.841717736231658	58.12956610970519	17.56983822633595	8.458877927727197
38	25.59126904632442	56.39490435443846	9.077231900114882	8.936594699122244
39	17.12114831909927	53.9807686804224	9.798815213347451	19.099267787130877
40	17.910801672928564	53.21257885290739	19.43757384475125	9.439045629412798
41	18.108470529556296	53.037804424348224	10.334994542131879	18.5187305039636
42	18.224373571653427	62.24770135853901	10.07518366646089	9.452741403346675
43	17.755037796247766	64.00157808022044	9.517131982289524	8.726252141242268
44	18.082101054370177	63.55084402762049	10.037571391776812	8.329483526232517
45	25.984358199680297	55.21625013797981	9.712961108090319	9.086430554249574
46	18.20842923781996	55.62139974897895	16.850299058466646	9.319871954734445
47	26.073687352054982	53.94438289295629	9.962755671481311	10.019174083507426
48	25.904432115976633	54.70337406633661	9.957236479000494	9.434957338686269
49	26.09228907486069	54.447447066855815	9.428007244451168	10.032256613832322
50	17.896697069922034	60.347872657920455	10.923912821288548	10.831517450868965
51	16.233580402369576	62.02754690291536	11.900401061320274	9.838471633394793
52	23.857629363739314	53.83195489797671	11.778161168597022	10.532254569686959
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	10.0
5	206.0
6	402.0
7	1091.5
8	1781.0
9	31758.0
10	61735.0
11	47251.0
12	32767.0
13	22892.0
14	9567.0
15	6117.0
16	5883.5
17	5650.0
18	5639.0
19	5628.0
20	6207.5
21	6787.0
22	8028.0
23	9269.0
24	10934.0
25	12599.0
26	13598.5
27	14598.0
28	15567.5
29	16537.0
30	18298.5
31	20060.0
32	20025.5
33	19991.0
34	18832.0
35	17673.0
36	17302.0
37	16931.0
38	16142.0
39	13704.5
40	12056.0
41	11966.0
42	11876.0
43	12720.0
44	13564.0
45	12320.0
46	11076.0
47	11064.0
48	11052.0
49	13669.5
50	16287.0
51	30812.0
52	45337.0
53	41317.5
54	37298.0
55	31572.5
56	25847.0
57	19878.0
58	13909.0
59	10575.5
60	7242.0
61	5115.0
62	2988.0
63	2148.5
64	1060.0
65	811.0
66	663.5
67	516.0
68	472.5
69	429.0
70	454.0
71	479.0
72	288.0
73	97.0
74	84.0
75	71.0
76	45.5
77	20.0
78	18.0
79	16.0
80	11.0
81	6.0
82	5.5
83	5.0
84	3.5
85	2.0
86	3.0
87	4.0
88	2.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	489202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.985276969883515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.02201990694992	31.075776778276087
2	7.313555411717412	5.409877450739178
3	2.7959739780930013	3.1022961594107
4	1.497327767430131	2.215163287724029
5	0.8975145711478328	1.659741249920441
6	0.598641880171496	1.3284561446348755
7	0.4688635067781875	1.2138732661483485
8	0.3351673906817924	0.9917007020511397
9	0.2504704364060661	0.8337346616921353
>10	1.508899800074932	10.456220045761635
>50	0.15198309782887193	3.8952317212079284
>100	0.1373693384222496	10.200417818633028
>500	0.012860108277827622	3.4124625346920094
>1k	0.007014604515178704	4.838934139095068
>5k	0.0	0.0
>10k+	0.0023382015050595678	19.366114040013397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43343	8.859939247999804	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15962	3.2628648288437088	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	15678	3.204811100526981	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14593	2.9830213286127205	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4048	0.8274700430497014	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3540	0.7236274585958357	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2879	0.5885094500840145	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	1759	0.3595651693983262	No Hit
CAAAGTGGGAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1443	0.2949701759191499	No Hit
CAAAGTGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	1406	0.28740683807506917	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	1379	0.2818876455942535	No Hit
CAAAGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1332	0.27228016238690766	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1275	0.26062853381629675	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA	1124	0.22976193883099413	No Hit
CAAAGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.22710454985874956	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1086	0.22199418645058688	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	976	0.1995085874546711	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGAGGGAAG	974	0.19909975838201804	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGGAG	966	0.19746444209140598	No Hit
CAAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	922	0.18847020249303967	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG	880	0.17988479196732637	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	861	0.17600091577712274	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	811	0.16578018896079738	Illumina Single End Adapter 2 (100% over 25bp)
CAAAGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	807	0.16496253081549136	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	792	0.16189631277059374	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA	744	0.15208441502692138	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGGAG	743	0.15188000049059489	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG	651	0.1330738631485562	No Hit
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAGAGTGCAGTGCTCAAAGTGG	615	0.12571493984080195	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAA	605	0.12367079447753689	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT	600	0.12264872179590434	Illumina Single End Adapter 2 (95% over 24bp)
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	596	0.12183106365059831	No Hit
CAAAGTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.11733394385141517	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	570	0.11651628570610914	No Hit
CAAAGTGGGGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	537	0.1097706060073344	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAA	533	0.10895294786202837	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGCGGGAAG	514	0.10506907167182472	No Hit
CAAAGTGGGAAGCAGGGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	513	0.10486465713549822	No Hit
CAAAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	500	0.10220726816325362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.004088290726530145	0.0	0.0	0.0	0.0
8	0.026982718795098958	0.0	0.0	0.0	0.0
9	0.04701534335509667	0.0	0.0	0.0	0.0
10	0.254700512262828	0.0	0.0	0.0	0.0
11	0.48528010923912823	0.0	0.0	0.0	0.0
12	0.677020944313392	0.0	0.0	0.0	0.0
13	0.7824988450578697	0.0	0.0	0.0	0.0
14	0.9476657904096876	0.0	0.0	0.0	0.0
15	1.0555966655900835	0.0	0.0	0.0	0.0
16	1.1819248490398648	0.0	0.0	0.0	0.0
17	1.2737069758504667	0.0	0.0	0.0	0.0
18	1.3650802735884153	0.0	0.0	0.0	0.0
19	1.4603374475165678	0.0	0.0	0.0	0.0
20	1.578080220440636	0.0	0.0	0.0	0.0
21	1.6751771251957268	0.0	0.0	0.0	0.0
22	1.7963949452373458	0.0	0.0	0.0	0.0
23	1.9441866550014104	0.0	0.0	0.0	0.0
24	2.092796022910781	0.0	0.0	0.0	0.0
25	2.2324111512217857	0.0	0.0	0.0	0.0
26	2.37263952314177	0.0	0.0	0.0	0.0
27	2.513481138670733	0.0	0.0	0.0	0.0
28	2.841362054938451	0.0	0.0	0.0	0.0
29	2.9480664429008874	0.0	0.0	0.0	0.0
30	3.0966758108102583	0.0	0.0	0.0	0.0
31	3.175170992759637	0.0	0.0	0.0	0.0
32	3.251622029345751	0.0	0.0	0.0	0.0
33	3.3256200914959466	0.0	0.0	0.0	0.0
34	3.4251699706869556	0.0	0.0	0.0	0.0
35	3.8417667957203774	0.0	0.0	0.0	0.0
36	3.929869460877102	0.0	0.0	0.0	0.0
37	4.002436621273012	0.0	0.0	0.0	0.0
38	4.079705316004432	0.0	0.0	0.0	0.0
39	4.1524768909366685	0.0	0.0	0.0	0.0
40	4.225044051332579	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGTA	55	1.8189894E-12	46.000004	16
TTCATCG	35	1.01796104E-7	46.000004	35
CCAACAG	55	1.8189894E-12	46.000004	24
TTACTGG	55	1.8189894E-12	46.000004	32
AGGATTA	35	1.01796104E-7	46.000004	42
TTATCGA	55	1.8189894E-12	46.000004	44
TTACACC	35	1.01796104E-7	46.000004	46
GGATTAC	35	1.01796104E-7	46.000004	43
CAACAGG	55	1.8189894E-12	46.000004	25
CTGACGC	35	1.01796104E-7	46.000004	20
GCAATGT	55	1.8189894E-12	46.000004	15
GATTGCG	35	1.01796104E-7	46.000004	40
GCGTGCG	35	1.01796104E-7	46.000004	22
ATTACAC	35	1.01796104E-7	46.000004	45
GGTTACT	55	1.8189894E-12	46.000004	30
TCTCGTT	35	1.01796104E-7	46.000004	44
ATTGCGC	35	1.01796104E-7	46.000004	41
CGGGAGT	35	1.01796104E-7	46.000004	17
GTCTCGT	35	1.01796104E-7	46.000004	43
TCGTTGC	35	1.01796104E-7	46.000004	46
>>END_MODULE