FastQCFastQC Report
Fri 17 Jun 2016
SRR1527295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487005
Sequences flagged as poor quality0
Sequence length52
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA397128.15433106436279No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG160343.292368661512716No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145002.977382162400797No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135032.772661471648135No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37470.7693966181045369No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32190.6609788400529768No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27200.5585158263262184No Hit
CAAAGTGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19390.3981478629582858No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA16690.3427069537273745No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA13780.2829539737785033No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12120.24886808143653555Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12080.24804673463311466No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA10890.2236116672313426No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10750.22073695341936944Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA10550.21663021940226487No Hit
CAAAGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10090.2071847311629244No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGGAG9310.19116846849621666No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGAGGGAAG9150.18788308128253303No Hit
CAAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8690.17843759304319257No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA8680.17823225634233736No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGGAG8680.17823225634233736No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG8630.1772055728380612No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC7490.1537971889405653Illumina Single End Adapter 2 (100% over 25bp)
CAAAGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.15338651553885482No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA7330.15051180172688167No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG7250.14886910812003984No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA6970.14311968049609347No Hit
CAAAGTGGGGAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.14229833369267256No Hit
CAAAGTGGGTAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.1388076097781337No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAA6510.13367419225675303No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT6200.12730875453024096Illumina Single End Adapter 2 (95% over 24bp)
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAGAGTGCAGTGCTCAAAGTGG6160.12648740772682004No Hit
CAAAGTGGGAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5700.1170419194874796No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGCGGGAAG5670.11642590938491391No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA5520.1133458588720855No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAA5430.11149782856438846No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAA5400.11088181846182277No Hit
CAAAGTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.10759643124813914No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAA5140.10554306423958687No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5110.10492705413702118Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA206.3078554E-446.043
TGTTGTT405.6006684E-946.031
TAGAAGA253.4142362E-546.018
CGCTGGA206.3078554E-446.037
CAAGATT206.3078554E-446.046
CGACAAT405.6006684E-946.030
GAGAATT206.3078554E-446.046
TGTGACT206.3078554E-446.028
TGTTGGT405.6006684E-946.036
TGTGAAC206.3078554E-446.026
ACTGTGT206.3078554E-446.022
ATGGGCG206.3078554E-446.011
AACGTGC253.4142362E-546.023
AAGTACG253.4142362E-546.024
GGTGAAC253.4142362E-546.09
GGTTGCG206.3078554E-446.035
GGGTCGC301.8591782E-646.07
GTGTTGT405.6006684E-946.030
CATGAAA206.3078554E-446.039
CCGTGGA206.3078554E-446.015
Produced by FastQC (version 0.11.5)