##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487005
Sequences flagged as poor quality	0
Sequence length	52
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14670280592602	31.0	31.0	33.0	28.0	33.0
2	31.19173109105656	31.0	31.0	34.0	28.0	34.0
3	32.13870904816172	33.0	31.0	34.0	30.0	34.0
4	35.971437664911036	37.0	35.0	37.0	35.0	37.0
5	36.03593392264966	37.0	35.0	37.0	35.0	37.0
6	33.14093079126498	35.0	33.0	35.0	28.0	35.0
7	35.3045143273683	35.0	35.0	36.0	35.0	37.0
8	36.31888994979518	37.0	35.0	37.0	35.0	37.0
9	38.39336557119537	39.0	39.0	39.0	37.0	39.0
10	37.3958460385417	39.0	37.0	39.0	34.0	39.0
11	37.327657826921694	39.0	37.0	39.0	34.0	39.0
12	37.12589193129434	39.0	37.0	39.0	33.0	39.0
13	36.829576698391186	39.0	37.0	39.0	33.0	39.0
14	37.86127452490221	40.0	38.0	40.0	33.0	40.0
15	37.946860915185674	40.0	38.0	40.0	33.0	40.0
16	37.43162801203273	40.0	37.0	40.0	32.0	40.0
17	37.879861603063624	40.0	38.0	40.0	33.0	40.0
18	37.98509666225193	40.0	38.0	40.0	34.0	40.0
19	37.20596297779284	40.0	37.0	40.0	32.0	40.0
20	37.82202030779971	40.0	38.0	40.0	33.0	40.0
21	33.736374369873	40.0	34.0	40.0	10.0	40.0
22	34.70253282820505	40.0	34.0	40.0	18.0	40.0
23	36.67413270910976	40.0	34.0	40.0	30.0	40.0
24	37.12076672724099	39.0	37.0	40.0	32.0	40.0
25	36.564786809170336	37.0	36.0	40.0	32.0	40.0
26	35.77640681307174	37.0	35.0	40.0	31.0	40.0
27	35.395710516319134	35.0	35.0	40.0	30.0	40.0
28	35.344891736224476	35.0	35.0	40.0	30.0	40.0
29	35.18940051950185	35.0	35.0	40.0	30.0	40.0
30	34.518896109896204	35.0	35.0	39.0	26.0	40.0
31	34.069615301690945	35.0	34.0	39.0	25.0	40.0
32	33.06262358702683	35.0	33.0	38.0	20.0	40.0
33	32.75657539450314	35.0	33.0	38.0	19.0	40.0
34	32.265305284339995	35.0	33.0	38.0	16.0	40.0
35	32.42142277800023	35.0	33.0	38.0	17.0	40.0
36	31.799012330468887	35.0	31.0	38.0	15.0	40.0
37	30.635761439820946	35.0	27.0	38.0	10.0	40.0
38	30.347865011652857	35.0	25.0	38.0	10.0	40.0
39	29.74635784026858	35.0	24.0	38.0	9.0	40.0
40	25.47901150912208	33.0	10.0	35.0	6.0	40.0
41	25.927811829447336	33.0	12.0	35.0	8.0	40.0
42	27.004788451863945	33.0	15.0	35.0	8.0	40.0
43	27.85202615989569	34.0	16.0	35.0	8.0	39.0
44	27.80795885052515	35.0	15.0	35.0	8.0	40.0
45	27.54072956129814	35.0	15.0	35.0	8.0	40.0
46	27.158546626831345	35.0	14.0	35.0	8.0	40.0
47	27.153840309647745	35.0	14.0	35.0	8.0	40.0
48	27.18198786460098	34.0	14.0	35.0	8.0	40.0
49	27.0487633597191	34.0	13.0	35.0	8.0	39.0
50	26.47053521011078	33.0	11.0	35.0	8.0	39.0
51	26.251576472520817	33.0	10.0	35.0	8.0	39.0
52	25.709124136302503	33.0	10.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	3.0
15	12.0
16	55.0
17	147.0
18	343.0
19	677.0
20	1240.0
21	2137.0
22	3516.0
23	6005.0
24	9028.0
25	13537.0
26	19044.0
27	25702.0
28	30244.0
29	28186.0
30	22642.0
31	20451.0
32	21319.0
33	26213.0
34	41088.0
35	42411.0
36	91122.0
37	46220.0
38	33107.0
39	2553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57556903933225	0.17556287923121938	0.09630291270110163	0.15256516873543394
8	98.29036662867938	0.9248365006519441	0.3295654048726399	0.455231465796039
9	94.79183991950802	3.1297419944353755	0.7057422408394165	1.372675845217195
10	33.862896684838965	47.373024917608646	10.624531575651174	8.139546821901213
11	18.092216712354084	65.03834662888471	9.458219114793483	7.411217543967721
12	35.30107493762898	51.083253765361746	6.4405909590250605	7.175080337984209
13	15.198406587201363	53.265572222051105	7.819221568567059	23.71679962218047
14	15.595527766655373	70.7239145388651	6.634223467931541	7.046334226547982
15	29.032556133920593	54.895945626841616	7.760905945524173	8.310592293713617
16	13.752014866377142	56.248909148776704	24.196260818677427	5.802815166168726
17	30.942187451874208	54.17870453075431	7.192944630958614	7.686163386412871
18	26.72375026950442	55.246250038500634	9.50092914857137	8.529070543423579
19	14.335376433506845	54.80539214176445	23.117832465785774	7.741398958942927
20	11.3306844898923	66.70978737384627	9.960267348384514	11.99926078787692
21	18.638617673329843	48.46623751296188	23.056642128930914	9.838502684777364
22	10.721861171856553	57.23000790546299	7.452284884138767	24.595846038541698
23	11.038490364575312	72.84771203581072	7.42805515343785	8.685742446176118
24	11.607478362645148	71.06785351279761	8.948368086569953	8.37630003798729
25	12.431289206476318	58.50001540025257	8.678144988244474	20.39055040502664
26	27.677949918378665	56.265130748144266	8.947136066364822	7.109783267112248
27	15.139885627457625	56.45321916612765	9.984702415786286	18.422192790628433
28	18.81808194987731	67.35680331824109	7.33852835186497	6.4865863800166315
29	30.338908224761553	55.189782445765445	8.151867023952526	6.319442305520477
30	19.185018634305603	66.68494163304278	8.238519111713433	5.891520620938183
31	29.58101046190491	54.878081333867215	9.587170562930565	5.953737641297317
32	17.556903933224504	56.00414780135728	19.73408897239248	6.704859293025739
33	27.08699089331732	55.63864847383498	8.654120594244413	8.6202400386033
34	15.39943121733863	57.86573033131077	9.293333744006734	17.441504707343867
35	14.482808184720897	69.48943029332348	8.709150830073613	7.3186106918820135
36	24.74594716686687	58.284822537756284	9.563146168930505	7.4060841264463395
37	15.840289114074805	58.3521729756368	17.472715885873864	8.334822024414535
38	25.59809447541606	56.46102196076016	9.010174433527377	8.930709130296403
39	16.72508495805998	54.496976417079914	9.653288980605948	19.124649644254166
40	20.099588299914785	52.19761604090307	18.532663935688547	9.170131723493599
41	18.151353682200387	53.15284237328159	10.263344318846828	18.432459625671193
42	18.13923881684993	62.44514943378405	10.018582971427398	9.397028777938624
43	17.72836007843862	64.22131189618176	9.449594973357563	8.600733052022052
44	17.92158191394339	63.807147770556774	10.0107801767949	8.260490138704942
45	26.01862403876757	55.34481165491114	9.601749468691287	9.034814837630003
46	18.045194607858235	55.77273333949343	16.871490025769756	9.310582026878574
47	25.878584408784306	54.405806921900194	9.743021118879684	9.972587550435827
48	25.845114526544904	54.80785618217472	9.810987566862764	9.536041724417615
49	25.980636749109355	54.681368774447904	9.3921006971181	9.945893779324647
50	17.71706655989158	61.13900267964395	10.749170953070298	10.394759807394175
51	16.222626051067238	62.08806891099681	11.860247841397932	9.829057196538022
52	23.78209669305243	54.029835422634264	11.748750012833543	10.439317871479759
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	187.5
6	367.0
7	1004.5
8	1642.0
9	29348.5
10	57055.0
11	44572.5
12	32090.0
13	23930.5
14	11649.5
15	7528.0
16	6767.0
17	6006.0
18	5743.5
19	5481.0
20	6057.5
21	6634.0
22	7879.5
23	9125.0
24	10643.0
25	12161.0
26	13359.0
27	14557.0
28	15517.0
29	16477.0
30	18071.5
31	19666.0
32	19687.5
33	19709.0
34	18793.5
35	17878.0
36	17481.0
37	17084.0
38	16440.0
39	14175.5
40	12555.0
41	12309.5
42	12064.0
43	12857.0
44	13650.0
45	12483.5
46	11317.0
47	11114.5
48	10912.0
49	13483.0
50	16054.0
51	29832.5
52	43611.0
53	39864.0
54	36117.0
55	31299.0
56	26481.0
57	20559.5
58	14638.0
59	11029.5
60	7421.0
61	5356.0
62	3291.0
63	2350.0
64	1137.0
65	865.0
66	701.5
67	538.0
68	462.5
69	387.0
70	422.5
71	458.0
72	274.0
73	90.0
74	79.0
75	68.0
76	43.0
77	18.0
78	16.0
79	14.0
80	10.0
81	6.0
82	4.0
83	2.0
84	2.0
85	2.0
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	487005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.812648398609134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.15594518214141	31.821591658249375
2	7.279576287212159	5.505201172784117
3	2.817658308629597	3.19629368794892
4	1.4546055571727596	2.2000995396814598
5	0.885503945795168	1.6741624678966867
6	0.6155988597571749	1.3966453943149653
7	0.4235626210597307	1.121121712544744
8	0.33816172489726903	1.02294323243261
9	0.26002185555635493	0.8848903500095741
>10	1.4769665896816735	10.524322225386614
>50	0.141751272363316	3.7452656931474246
>100	0.12692373341317836	9.634079260253118
>500	0.01423443739213215	3.7098314428769283
>1k	0.007117218696066075	4.781381112449287
>5k	0.0	0.0
>10k+	0.0023724062320220254	18.782171050024175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39712	8.15433106436279	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	16034	3.292368661512716	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14500	2.977382162400797	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13503	2.772661471648135	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3747	0.7693966181045369	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3219	0.6609788400529768	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2720	0.5585158263262184	No Hit
CAAAGTGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1939	0.3981478629582858	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	1669	0.3427069537273745	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	1378	0.2829539737785033	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1212	0.24886808143653555	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1208	0.24804673463311466	No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA	1089	0.2236116672313426	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1075	0.22073695341936944	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	1055	0.21663021940226487	No Hit
CAAAGTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1009	0.2071847311629244	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGGAG	931	0.19116846849621666	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGAGGGAAG	915	0.18788308128253303	No Hit
CAAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	869	0.17843759304319257	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	868	0.17823225634233736	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGGAG	868	0.17823225634233736	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG	863	0.1772055728380612	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	749	0.1537971889405653	Illumina Single End Adapter 2 (100% over 25bp)
CAAAGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	747	0.15338651553885482	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	733	0.15051180172688167	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG	725	0.14886910812003984	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA	697	0.14311968049609347	No Hit
CAAAGTGGGGAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	693	0.14229833369267256	No Hit
CAAAGTGGGTAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	676	0.1388076097781337	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAA	651	0.13367419225675303	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT	620	0.12730875453024096	Illumina Single End Adapter 2 (95% over 24bp)
CAAAGTGGGAGAGTGCAGTGCTCAAAGTGGGAGAGTGCAGTGCTCAAAGTGG	616	0.12648740772682004	No Hit
CAAAGTGGGAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	570	0.1170419194874796	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGCGGGAAG	567	0.11642590938491391	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	552	0.1133458588720855	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAA	543	0.11149782856438846	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	540	0.11088181846182277	No Hit
CAAAGTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	524	0.10759643124813914	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAA	514	0.10554306423958687	No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	511	0.10492705413702118	Illumina Single End Adapter 2 (100% over 34bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005544090923091139	0.0	0.0	0.0	0.0
8	0.030595168427428876	0.0	0.0	0.0	0.0
9	0.0540035523249248	0.0	0.0	0.0	0.0
10	0.28418599398363464	0.0	0.0	0.0	0.0
11	0.5065656410098459	0.0	0.0	0.0	0.0
12	0.7160090758821778	0.0	0.0	0.0	0.0
13	0.8221681502243303	0.0	0.0	0.0	0.0
14	0.9716532684469359	0.0	0.0	0.0	0.0
15	1.0813030667036272	0.0	0.0	0.0	0.0
16	1.1907475282594635	0.0	0.0	0.0	0.0
17	1.2763729325160933	0.0	0.0	0.0	0.0
18	1.3605609798667364	0.0	0.0	0.0	0.0
19	1.4492664346361948	0.0	0.0	0.0	0.0
20	1.5531668052689398	0.0	0.0	0.0	0.0
21	1.641050913234977	0.0	0.0	0.0	0.0
22	1.7585035061241672	0.0	0.0	0.0	0.0
23	1.9042925637313786	0.0	0.0	0.0	0.0
24	2.0562417223642466	0.0	0.0	0.0	0.0
25	2.201825443270603	0.0	0.0	0.0	0.0
26	2.347409164176959	0.0	0.0	0.0	0.0
27	2.4977156292029856	0.0	0.0	0.0	0.0
28	2.823174300058521	0.0	0.0	0.0	0.0
29	2.916397162246794	0.0	0.0	0.0	0.0
30	3.0515087114095336	0.0	0.0	0.0	0.0
31	3.1239925668114292	0.0	0.0	0.0	0.0
32	3.1977084424184556	0.0	0.0	0.0	0.0
33	3.2650588802989704	0.0	0.0	0.0	0.0
34	3.359924436094085	0.0	0.0	0.0	0.0
35	3.7215223663001407	0.0	0.0	0.0	0.0
36	3.7972916089157196	0.0	0.0	0.0	0.0
37	3.856428578762025	0.0	0.0	0.0	0.0
38	3.921520312933132	0.0	0.0	0.0	0.0
39	3.9993429225572634	0.0	0.0	0.0	0.0
40	4.071826777959158	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACA	20	6.3078554E-4	46.0	43
TGTTGTT	40	5.6006684E-9	46.0	31
TAGAAGA	25	3.4142362E-5	46.0	18
CGCTGGA	20	6.3078554E-4	46.0	37
CAAGATT	20	6.3078554E-4	46.0	46
CGACAAT	40	5.6006684E-9	46.0	30
GAGAATT	20	6.3078554E-4	46.0	46
TGTGACT	20	6.3078554E-4	46.0	28
TGTTGGT	40	5.6006684E-9	46.0	36
TGTGAAC	20	6.3078554E-4	46.0	26
ACTGTGT	20	6.3078554E-4	46.0	22
ATGGGCG	20	6.3078554E-4	46.0	11
AACGTGC	25	3.4142362E-5	46.0	23
AAGTACG	25	3.4142362E-5	46.0	24
GGTGAAC	25	3.4142362E-5	46.0	9
GGTTGCG	20	6.3078554E-4	46.0	35
GGGTCGC	30	1.8591782E-6	46.0	7
GTGTTGT	40	5.6006684E-9	46.0	30
CATGAAA	20	6.3078554E-4	46.0	39
CCGTGGA	20	6.3078554E-4	46.0	15
>>END_MODULE