FastQCFastQC Report
Fri 17 Jun 2016
SRR1527294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8328
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC4955.943804034582133No Hit
CGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAA2382.8578290105667628No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG2022.425552353506244No Hit
CGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGC1962.353506243996158No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGA1732.0773294908741593No Hit
CGAGTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG1551.8611911623439No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1351.6210374639769451No Hit
CGAGTAGGGGTACGTGTCTCAAAAGTGAACAATATTGAGGTGGTCAAAAT1111.3328530259365994No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1091.308837656099904No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1061.2728146013448607No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT1051.260806916426513No Hit
CGAGTAAGGGGATATGCCCTTCAAAGCGTTATTAACGCACCTTATCTGCC1051.260806916426513No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT1041.248799231508165No Hit
CGAGTAGGGAAAGAATGAGGAAAATTAGGAGGACGTCCCAGGATAGAAAT760.9125840537944284No Hit
CGAGTAGGGGTGCTCGAGTAGGGGATACGGGTTGACTTGAGGAAGGTAGG740.888568683957733No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAA730.8765609990393852No Hit
CGAGTAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC620.74447646493756No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAA580.696445725264169No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT530.6364073006724303No Hit
CGAGTAGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT520.6243996157540825No Hit
CGAGTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGC460.5523535062439962No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAAT460.5523535062439962No Hit
CGAGTAGGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT420.5043227665706052No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAAT370.4442843419788665No Hit
CGAGTAGGGGTGCTCGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTC350.42026897214217096No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT350.42026897214217096No Hit
CGAGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA350.42026897214217096No Hit
CGAGTAGGGATACCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.40826128722382327No Hit
CGAGTAGGGGAACCGCCCCCGCCGCTAAATCCGCCGCTATCTGAGCCTCC280.33621517771373677No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.3242074927953891No Hit
CGAGTAGGGGTGCACTGCTCGAGTAGGGGTGAGTGCAGTGCTCGAGTAGG260.31219980787704127No Hit
CGAGTAGGGGGTGCTCGAGTAGGGTATCAACGCGAGTTCAGTGCCGAGTA230.2761767531219981No Hit
CGAGTAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.2641690682036503No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGCTGCAATAA220.2641690682036503No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG220.2641690682036503No Hit
CGAGTAGGGGCTCGAGTAGGGTGCTCGAGTAGGGAAGGCAGAGTGCAGTG200.24015369836695488No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT200.24015369836695488No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2281460134486071No Hit
CGAGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.21613832853025938No Hit
CGAGTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.20413064361191163No Hit
CGAGTAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.19212295869356388No Hit
CGAGTAGGGAAGCAGGTGATATCAACGCAAAGTTCAGTGCTCGAGTACTG160.19212295869356388No Hit
CGAGTAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.18011527377521613No Hit
CGAGTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCAT140.16810758885686838No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCGTGGGCTGCAATAA140.16810758885686838No Hit
CGAGTAGGGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.16810758885686838No Hit
CGAGTAGGGGCATCTAGACTGATGAAAAAATGCCGTGCGAAAAAACATGA140.16810758885686838No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTT140.16810758885686838No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCCTTT130.15609990393852063No Hit
CGAGTAGGGCAGTGAGCCGAGATCCCGCCATTGCACTCCAGCCTGAGCAA130.15609990393852063No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCCCGTCTGGCAGCATTT130.15609990393852063No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGGTGCAATAA130.15609990393852063No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGCTGCAAT120.1440922190201729No Hit
CGAGTAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA120.1440922190201729Illumina Single End Adapter 2 (97% over 34bp)
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGGAGCATTT120.1440922190201729No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGGAGGGA120.1440922190201729No Hit
CGAGTAGGGTGCAGTGCTCGAGTAGGGACGTGGGGAAGTGAAACATCTCA120.1440922190201729No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT120.1440922190201729No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT110.13208453410182516No Hit
CGAGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG110.13208453410182516No Hit
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC110.13208453410182516No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCT110.13208453410182516No Hit
CGAGTAGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA110.13208453410182516No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGG110.13208453410182516No Hit
CGAGTAGGGGAGTCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA100.12007684918347744Illumina Single End Adapter 2 (100% over 34bp)
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCCGGCAGCATTT100.12007684918347744No Hit
CGAGTAGGGTGAGCCGAGATTGCACCGCTGCACTCTAGCCTGGGCAACTG100.12007684918347744No Hit
CGAGTAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA100.12007684918347744Illumina Single End Adapter 2 (97% over 34bp)
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTT100.12007684918347744No Hit
CGAGTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCAT100.12007684918347744No Hit
CGAGTAGGGGAGTCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA100.12007684918347744Illumina Single End Adapter 2 (97% over 34bp)
CGAGTAGGGGTGATATCAACACAGAGTGCCGTGCTCGAGTAGGGGTTGAC100.12007684918347744No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCCTTT100.12007684918347744No Hit
CGAGTAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12007684918347744No Hit
CGAGTAGGGTCAGCGCAGAGTGCAGTGCTCGAGTAGGGGTGGTATCAACG90.10806916426512969No Hit
CGAGTAGGGGTGCTCGAGTAGGGTCCCGGCGCGCGGGTAGGGAATCGACC90.10806916426512969No Hit
CGAGTAGAGGCAGTGCTCGAGTAGGGGTGCAGGCTCGAGTAGGGTCAAAA90.10806916426512969No Hit
CGAGTAGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA90.10806916426512969No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC90.10806916426512969Illumina Single End Adapter 2 (95% over 22bp)
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGTGGGCTGCAAT90.10806916426512969No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCAATTT90.10806916426512969No Hit
CGAGTAGGGGCTCGAGTAGGGAGAGTGCAGTGCTCGAGTAGGGGGTATCA90.10806916426512969No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGGCGCATTT90.10806916426512969No Hit
CGAGTAGGGGTGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA90.10806916426512969No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTT90.10806916426512969No Hit
CGAGTAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA90.10806916426512969Illumina Single End Adapter 2 (97% over 34bp)
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTCGACTTTTTTTTTTTTTTTT90.10806916426512969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA302.236662E-644.0000046
CGAGTAA551.8189894E-1244.0000041
GGGGTGG302.236662E-644.0000047
AGCGCCG302.236662E-644.00000444
GGGTGGT302.236662E-644.0000048
AGGGGTC351.2281453E-744.06
CTTTCCT351.2281453E-744.09
GAGGTGG207.412923E-444.037
TCCTTTC351.2281453E-744.07
TCCTTTA351.2281453E-744.012
AACAATA254.0715786E-544.028
GTGTCTC254.0715786E-544.014
TGGTCAA207.412923E-444.041
CCAACCA351.2281453E-744.043
CTTTATC351.2281453E-744.014
TTTCCTT351.2281453E-744.010
ACTTGCC351.2281453E-744.029
TGTCTCA254.0715786E-544.015
GGGTACG254.0715786E-544.08
CAACCAA351.2281453E-744.044
Produced by FastQC (version 0.11.5)