FastQCFastQC Report
Fri 17 Jun 2016
SRR1527293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8917
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4855.439049007513738No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1992.231692273186049No Hit
CGAGTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1761.9737579903555007No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAG1521.7046091734888418No Hit
CGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAAT1421.5924638331277337No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1241.390602220477739No Hit
CGAGTAGGGGTACGTGTCTCAAAAGTGAACAATATTGAGGTGGTCAAAATAA1091.222384209936077No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1061.188740607827745No Hit
CGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGT951.065380733430526No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA941.0541661993944151No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAAAG931.0429516653583044No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA921.0317371313221935No Hit
CGAGTAGGGAAAGAATGAGGAAAATTAGGAGGACGTCCCAGGATAGAAATTC820.9195917909610855No Hit
CGAGTAAGGGGATATGCCCTTCAAAGCGTTATTAACGCACCTTATCTGCCTG810.9083772569249748No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAG810.9083772569249748No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.785017382527756No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAA630.7065156442749804No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAA600.672872042166648No Hit
CGAGTAGGGGTGCTCGAGTAGGGGATACGGGTTGACTTGAGGAAGGTAGGGG590.6616575081305371No Hit
CGAGTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC510.5719412358416508No Hit
CGAGTAGGGGGGAATACCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG510.5719412358416508No Hit
CGAGTAGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.5382976337333184No Hit
CGAGTAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG480.5382976337333184No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT420.47101042951665356No Hit
CGAGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.4037232252999888No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT330.3700796231916564No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA320.3588650891555456No Hit
CGAGTAGGGATACCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.32522148704721315No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.3140069530111024No Hit
CGAGTAGGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.3140069530111024No Hit
CGAGTAGGGGTGCACTGCTCGAGTAGGGGTGAGTGCAGTGCTCGAGTAGGGG270.3027924189749916No Hit
CGAGTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT240.2691488168666592No Hit
CGAGTAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.2691488168666592No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.2691488168666592No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG240.2691488168666592No Hit
CGAGTAGGGGGAATTACTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA220.2467197487944376No Hit
CGAGTAGGGGAACCGCCCCCGCCGCTAAATCCGCCGCTATCTGAGCCTCCAG210.23550521475832678No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTGA210.23550521475832678No Hit
CGAGTAGGGGTGCTCGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCC210.23550521475832678No Hit
CGAGTATCCTTTCCTATATCAGCGGCAGACTTGCCACCAAGTCCAACCAAAT190.21307614668610522No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTA180.2018616126499944No Hit
CGAGTAGGGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.2018616126499944No Hit
CGAGTAGGGGTGCTCGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCT180.2018616126499944No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGA170.19064707861388358No Hit
CGAGTAGGGGCTCGAGTAGGGTGCTCGAGTAGGGAAGGCAGAGTGCAGTGCT160.1794325445777728No Hit
CGAGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1794325445777728No Hit
CGAGTAGGGTGCAGTGCTCGAGTAGGGACGTGGGGAAGTGAAACATCTCAGT160.1794325445777728No Hit
CGAGTAGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAG160.1794325445777728No Hit
CGAGTAGGGGGGAATACGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA160.1794325445777728No Hit
CGAGTAGGGGGTGCTCGAGTAGGGTATCAACGCGAGTTCAGTGCCGAGTAGG160.1794325445777728No Hit
CGAGTAGGGGTGCTCGAGTAGGGTCCCGGCGCGCGGGTAGGGAATCGACCTA150.168218010541662No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTA150.168218010541662No Hit
CGAGTAACAAAGTTTAGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGT150.168218010541662No Hit
CGAGTAGGGAAGCAGGTGATATCAACGCAAAGTTCAGTGCTCGAGTACTGCA140.1570034765055512No Hit
CGAGTAGGGAGAGTGCAGTGCTCGAGTAGGGGTGCTCGAGTAGGGGATCGAG140.1570034765055512No Hit
CGAGTAGGGGGGGAAATCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAA140.1570034765055512No Hit
CGAGTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1457889424694404No Hit
CGAGTAGGGCAGTGAGCCGAGATCCCGCCATTGCACTCCAGCCTGAGCAACA130.1457889424694404No Hit
CGAGTAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1457889424694404No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTA130.1457889424694404No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA130.1457889424694404No Hit
CGAGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG130.1457889424694404No Hit
CGAGTAGGGGCTCGAGTAGGGGGAGTGCAGTGCTCGAGTAGGTGGTATCAAC120.1345744084333296No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT120.1345744084333296No Hit
CGAGTAGGGGAATGCAGTACTCGAGTAGGGGTGCAGTGCTCGAGTAGGGTCA120.1345744084333296No Hit
CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGGTGGTATCAACGCAAAAAAAAA120.1345744084333296No Hit
CGAGTAGGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCGGT120.1345744084333296No Hit
CGAGTAGGGCAGAGTGCAGTGCTCGAATAGGGGTATCAACGCAAAAAAAAAA110.1233598743972188No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCTGC110.1233598743972188Illumina Single End Adapter 1 (95% over 21bp)
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGGAG110.1233598743972188No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA110.1233598743972188No Hit
CGAGTAGGGAACGCAGAGTGCAGTGCTCGAGTAGGGCACTTTGCGTTGATAC110.1233598743972188No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTGAT110.1233598743972188No Hit
CGAGTAGGGGGAATTAGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAAAG100.112145340361108No Hit
CGAGTAGGGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGTGGTAGCAGTGCTC100.112145340361108No Hit
CGAGTAGGGAAAGAAAGAGGAAAATTAGGAGGACGTCCCAGGATAGAAATTC100.112145340361108No Hit
CGAGTAGGGGCTCGAGTAGGGTGGTATCAACGCAAAATCAGTGCTCGAGTAG100.112145340361108No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGTGGGCTGCAATAA100.112145340361108No Hit
CGAGTAAGGGGATATACCCTTCAAAGCGTTATTAACGCACCTTATCTGCCTG100.112145340361108No Hit
CGAGTAGGGGTCGACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.112145340361108No Hit
CGAGTAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.112145340361108No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT100.112145340361108No Hit
CGAGTAGGGGAGTCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA100.112145340361108Illumina Single End Adapter 2 (100% over 34bp)
CGAGTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG90.1009308063249972No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGCTGCAATAA90.1009308063249972No Hit
CGAGTAGGGGCATCTAGACTGATGAAAAAATGCCGTGCGAAAAAACATGATG90.1009308063249972No Hit
CGAGTAGGGGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.1009308063249972No Hit
CGAGTAGGGGGGAATGCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG90.1009308063249972No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAA90.1009308063249972No Hit
CGAGTAGGGGGGAATACGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA90.1009308063249972No Hit
CGAGTAGGGAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGC90.1009308063249972Illumina Single End Adapter 2 (100% over 31bp)
CGAGTAGGGGTGGTGAGTGCAGTGCTCAAGTGGGCAAAAAAAAAAAAAAAAA90.1009308063249972No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCTGCT90.1009308063249972Illumina Single End Adapter 1 (95% over 22bp)
CGAGTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT90.1009308063249972No Hit
CGAGTAGGGGGAATTAGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAG90.1009308063249972No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTT90.1009308063249972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTC501.2732926E-1146.06
CTTTCCT301.6606591E-646.09
TCCTCTT205.9783406E-446.011
TCCTTTC301.6606591E-646.07
TCCTTTA301.6606591E-646.012
CCAACCA301.6606591E-646.043
GGGTCCT253.1499818E-546.08
CTTTATC301.6606591E-646.014
TTTCCTT301.6606591E-646.010
ACTTGCC301.6606591E-646.029
AGTAGGA205.9783406E-446.03
GGTCCTC253.1499818E-546.09
CAACCAA301.6606591E-646.044
CTCTTTT205.9783406E-446.013
TTATCAG301.6606591E-646.016
GCGGCAG301.6606591E-646.022
GTATCCT301.6606591E-646.04
ACCAAGT301.6606591E-646.036
ACCAAAT301.6606591E-646.046
AGGGATA205.9783406E-446.06
Produced by FastQC (version 0.11.5)