FastQCFastQC Report
Fri 17 Jun 2016
SRR1527292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8596
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4244.932526756630992No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1621.8845974872033502No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGAGTAGGGAAG1601.8613308515588647No Hit
CGAGTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1461.698464402047464No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1401.6286644951140066No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA1151.337831549557934No Hit
CGAGTAGGGGTACGTGTCTCAAAAGTGAACAATATTGAGGTGGTCAAAATAA1141.326198231735691No Hit
CGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGT1141.326198231735691No Hit
CGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAAT1131.314564913913448No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1061.233131689157748No Hit
CGAGTAGGGAAAGAATGAGGAAAATTAGGAGGACGTCCCAGGATAGAAATTC961.116798510935319No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAAAG931.0818985574685902No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA881.0237319683573756No Hit
CGAGTAAGGGGATATGCCCTTCAAAGCGTTATTAACGCACCTTATCTGCCTG730.849232201023732No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAG730.849232201023732No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAA670.7794322940902746No Hit
CGAGTAGGGGTGCTCGAGTAGGGGATACGGGTTGACTTGAGGAAGGTAGGGG590.6863657515123314No Hit
CGAGTAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAA580.6747324336900884No Hit
CGAGTAGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.6630991158678455No Hit
CGAGTAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG510.5932992089343881No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.5932992089343881No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT430.5002326663564449No Hit
CGAGTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC420.4885993485342019No Hit
CGAGTAGGGATACCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.4885993485342019No Hit
CGAGTAGGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.4304327594229875No Hit
CGAGTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT310.36063285248953003No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT300.3489995346672871No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG290.3373662168450442No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA270.3140995812005584No Hit
CGAGTAGGGGTGCACTGCTCGAGTAGGGGTGAGTGCAGTGCTCGAGTAGGGG250.2908329455560726No Hit
CGAGTAGGGGAACCGCCCCCGCCGCTAAATCCGCCGCTATCTGAGCCTCCAG230.2675663099115868No Hit
CGAGTAGGGGGTGCTCGAGTAGGGTATCAACGCGAGTTCAGTGCCGAGTAGG230.2675663099115868No Hit
CGAGTAGGGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.23266635644485809No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTA190.22103303862261517No Hit
CGAGTAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.22103303862261517No Hit
CGAGTAGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCATTGTAGCGCCGG190.22103303862261517No Hit
CGAGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.20939972080037225No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.1977664029781294No Hit
CGAGTAGGGGTGCTCGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCC170.1977664029781294No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTA170.1977664029781294No Hit
CGAGTAGGGGCATCTAGACTGATGAAAAAATGCCGTGCGAAAAAACATGATG160.18613308515588647No Hit
CGAGTAGGGAAGCAGGTGATATCAACGCAAAGTTCAGTGCTCGAGTACTGCA160.18613308515588647No Hit
CGAGTAGGGGAGTCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA160.18613308515588647Illumina Single End Adapter 2 (100% over 34bp)
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCTGC150.17449976733364356Illumina Single End Adapter 1 (95% over 21bp)
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTA150.17449976733364356No Hit
CGAGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.17449976733364356No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTA150.17449976733364356No Hit
CGAGTAGGGGCTCGAGTAGGGTGCTCGAGTAGGGAAGGCAGAGTGCAGTGCT130.15123313168915775No Hit
CGAGTAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.15123313168915775No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGA130.15123313168915775No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT130.15123313168915775No Hit
CGAGTAGGGTGCAGTGCTCGAGTAGGGACGTGGGGAAGTGAAACATCTCAGT120.13959981386691486No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA120.13959981386691486No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTGA120.13959981386691486No Hit
CGAGTAGGGCAGAGTGCAGTGCTCGAGTAGGGAAGCAGTGGTAGCAGTGCTC110.12796649604467195No Hit
CGAGTAGGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA110.12796649604467195No Hit
CGAGTAGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCGTTGTAGCGCCGG110.12796649604467195No Hit
CGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGCAGCATTTGA110.12796649604467195No Hit
CGAGTAGGGGTGCTCGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCT110.12796649604467195No Hit
CGAGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGATGTAGCGCCGGA110.12796649604467195No Hit
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT100.11633317822242904No Hit
CGAGTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.11633317822242904No Hit
CGAGTAGGGCAGTGAGCCGAGATCCCGCCATTGCACTCCAGCCTGAGCAACA100.11633317822242904No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA100.11633317822242904No Hit
CGAGTAGGGGTGATATCAACACAGAGTGCCGTGCTCGAGTAGGGGTTGACTT100.11633317822242904No Hit
CGAGTAGGGGTGCTCGAGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT100.11633317822242904No Hit
CGAGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10469986040018613No Hit
CGAGTAGGGGCTCGAGTAGGGTGGTATCAACGCAAAATCAGTGCTCGAGTAG90.10469986040018613No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT90.10469986040018613No Hit
CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGGTGGTATCAACGCAAAAAAAAA90.10469986040018613No Hit
CGAGTAGGGGAGTCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA90.10469986040018613Illumina Single End Adapter 2 (97% over 34bp)
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCGTGGGCTGCAATAAAG90.10469986040018613No Hit
CGAGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTAT90.10469986040018613No Hit
CGAGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG90.10469986040018613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGG358.699135E-846.00000437
AACAATA358.699135E-846.00000428
GTGTCTC358.699135E-846.00000414
TGGTCAA358.699135E-846.00000441
TGTCTCA358.699135E-846.00000415
GGGTACG358.699135E-846.0000048
GTCAAAA358.699135E-846.00000443
GGTCAAA358.699135E-846.00000442
TATTGAG358.699135E-846.00000433
TTGAGGT358.699135E-846.00000435
CAATATT358.699135E-846.00000430
TACGTGT358.699135E-846.00000411
TCAAAAT358.699135E-846.00000444
TCAAAAG358.699135E-846.00000419
GTCTCAA358.699135E-846.00000416
ATTGAGG358.699135E-846.00000434
TGAGGTG358.699135E-846.00000436
AGTGAAC358.699135E-846.00000424
GAACAAT358.699135E-846.00000427
CGTGTCT358.699135E-846.00000413
Produced by FastQC (version 0.11.5)