FastQCFastQC Report
Fri 17 Jun 2016
SRR1527288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907018
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC203012.2382135745927867No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGA110191.21486012405487No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG77130.8503690114198394No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC30390.3350539901082448No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT30330.3343924817368564No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG28680.31620100152367425No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT28280.311790945714418No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC24920.27474647691666537No Hit
TTCCGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC21640.23858401928076398No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20510.22612561161961506No Hit
TTCCGCGGGGTTCATAATTCTGAGGTCAGGAGGTGGAATCTAGAGATGGC18990.20936739954444125No Hit
TTCCGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC17520.19316044444542446No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC16790.18511209259353179No Hit
TTCCGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC16720.18434033282691192No Hit
TTCCGCGAGGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGG16490.18180455073658958No Hit
TTCCGCGGGGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTAC15860.17485871283701096No Hit
TTCCGCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG15340.16912564028497779No Hit
TTCCGCGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTC15110.16658985819465547No Hit
TTCCGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT15050.165928349823267No Hit
TTCCGCGGGACCCCATACTCTATCCCTCTTCCTGCTCCCAACACATACTT14720.1622900537806306No Hit
TTCCGCGGGTGAATGTGGGAAGGACTGTAATCATAATAAGCTCACTACTT13630.15027265170040727No Hit
TTCCGCGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCAT13510.1489496349576304No Hit
TTCCGCGGGCTAACCATACGCCTTGCTTTGGGTGGCTGCACCAACCGGCC13130.14476008193883694No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT13050.14387807077698567No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT12870.14189354566282036No Hit
TTCCGCGGGGCTGATTCAAGACTAAGGGAGATGCTTTGGTTTTCTTAGAC12310.1357194675298616No Hit
TTCCGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT12120.13362469102046484No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC11930.13152991451106813No Hit
TTCCGCGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11750.12954538939690283No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT11220.12370206544963826No Hit
TTCCGCGGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAA10940.12061502638315888No Hit
TTCCGCAGGTACAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATT10850.11962276382607621No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC10730.11829974708329934No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGG10510.11587421638820838No Hit
TTCCGCGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGC10420.11488195383112573No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT10390.11455119964543152No Hit
TTCCGCGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGC10360.1142204454597373No Hit
TTCCGCGGGCGTCTTTAAAATAAAGGGGAGGGGAGGGGGAGATGATGCAT10200.1124564231360348No Hit
TTCCGCGGGGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCA10180.11223592034557198No Hit
TTCCGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT10030.11058214941710087No Hit
TTCCGCGGGGGGCAGGCAGCTTTTACATCCTGTAGTGCCTGATAGCACCA10010.11036164662663805No Hit
TTCCGCGGGGAGCCTTTGCTGAGGAGCTGGGCTTCCACAGGGACTCAAGT9760.10760536174585289No Hit
TTCCGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT9600.10584133942215038No Hit
TTCCGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG9540.10517983105076195No Hit
TTCCGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT9230.10176203779858833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.855601E-444.00000429
AACGTTG207.855601E-444.00000429
CGGAATT408.309144E-944.00000432
TAATAGG650.044.00000413
AACCGAA207.855601E-444.00000435
GATAATC408.309144E-944.00000413
CGTATAG207.855601E-444.00000426
CGTATAC207.855601E-444.00000418
GTACACG408.309144E-944.00000432
TTTACGC207.855601E-444.00000413
ACGCATA207.855601E-444.00000426
TCATTCG207.855601E-444.00000443
ACGCAAT207.855601E-444.00000418
ACGCAAC207.855601E-444.00000415
ACACCTA207.855601E-444.00000440
CTATACC207.855601E-444.00000428
CGCATAA207.855601E-444.00000427
ATATGTG207.855601E-444.00000412
GTAGGGT207.855601E-444.00000444
CGATTAT207.855601E-444.00000442
Produced by FastQC (version 0.11.5)