##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907018
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47315929783091	34.0	31.0	34.0	31.0	34.0
2	32.884185319365216	34.0	31.0	34.0	31.0	34.0
3	32.901987612153235	34.0	31.0	34.0	31.0	34.0
4	36.44650933057558	37.0	37.0	37.0	35.0	37.0
5	36.124267655107175	37.0	37.0	37.0	35.0	37.0
6	36.13620236864097	37.0	37.0	37.0	35.0	37.0
7	36.50931183284124	37.0	37.0	37.0	35.0	37.0
8	36.635353432897695	37.0	37.0	37.0	35.0	37.0
9	38.71971118544505	39.0	39.0	39.0	39.0	39.0
10	38.052877671666934	39.0	38.0	39.0	37.0	39.0
11	37.72467249823047	39.0	38.0	39.0	35.0	39.0
12	36.999007737442916	39.0	35.0	39.0	33.0	39.0
13	36.794152927505294	39.0	35.0	39.0	33.0	39.0
14	37.5467146186735	40.0	36.0	40.0	33.0	40.0
15	37.49130557497205	40.0	36.0	40.0	33.0	40.0
16	37.15077650057661	39.0	35.0	40.0	33.0	40.0
17	37.07469201272742	39.0	35.0	40.0	32.0	40.0
18	37.086074366771115	39.0	35.0	40.0	32.0	40.0
19	36.707692680850876	39.0	35.0	40.0	31.0	40.0
20	36.57277694599225	39.0	35.0	40.0	31.0	40.0
21	36.78622915973001	39.0	35.0	40.0	32.0	40.0
22	36.612788279835684	38.0	35.0	40.0	32.0	40.0
23	36.63283859857246	38.0	35.0	40.0	32.0	40.0
24	36.65190547486378	38.0	35.0	40.0	32.0	40.0
25	36.56240449472888	38.0	35.0	40.0	32.0	40.0
26	36.37018339217083	38.0	35.0	40.0	32.0	40.0
27	36.132428463382205	38.0	35.0	40.0	31.0	40.0
28	35.884822572429655	37.0	34.0	40.0	31.0	40.0
29	36.11423036808531	37.0	35.0	40.0	31.0	40.0
30	36.041270404776974	37.0	35.0	40.0	31.0	40.0
31	35.9007814618894	37.0	35.0	40.0	31.0	40.0
32	34.94272550269124	36.0	34.0	40.0	28.0	40.0
33	35.145678476061114	36.0	34.0	40.0	29.0	40.0
34	34.83856549704637	35.0	34.0	40.0	28.0	40.0
35	34.90254107415729	35.0	34.0	40.0	28.0	40.0
36	34.7591348793519	35.0	34.0	39.0	29.0	40.0
37	34.656227329556856	35.0	34.0	39.0	28.0	40.0
38	34.421219865537395	35.0	34.0	39.0	28.0	40.0
39	34.377198688449404	35.0	34.0	39.0	28.0	40.0
40	34.204759993737724	35.0	34.0	39.0	28.0	40.0
41	34.16200891272279	35.0	34.0	38.0	28.0	40.0
42	34.00684661164387	35.0	34.0	38.0	27.0	40.0
43	33.76182721842345	35.0	34.0	37.0	26.0	40.0
44	33.718175383509475	35.0	33.0	37.0	26.0	40.0
45	33.544821602217375	35.0	33.0	37.0	26.0	40.0
46	33.22326899796917	35.0	33.0	36.0	24.0	40.0
47	33.15525711727882	35.0	33.0	36.0	24.0	39.0
48	32.97795302849558	35.0	33.0	36.0	24.0	39.0
49	32.77395817944076	35.0	33.0	36.0	24.0	39.0
50	32.41533464605995	35.0	32.0	35.0	23.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	3.0
15	6.0
16	40.0
17	100.0
18	211.0
19	412.0
20	712.0
21	1220.0
22	1973.0
23	3015.0
24	4137.0
25	6116.0
26	8266.0
27	11381.0
28	14420.0
29	17590.0
30	22425.0
31	26018.0
32	32600.0
33	46617.0
34	97872.0
35	130071.0
36	134908.0
37	143759.0
38	157928.0
39	45215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.43198481176779	0.3994408049233863	0.07067114434333166	0.09790323896548911
8	99.23518607127974	0.5856554114692322	0.043328798325942816	0.135829718925093
9	98.51601622018526	0.7811311352145162	0.35710426915452614	0.34574837544569126
10	54.15239829860047	34.79159178759407	3.2216560200569337	7.834353893748526
11	37.22186329267997	23.81055282254597	21.062206042217465	17.905377842556597
12	37.955145322364054	12.920471258563776	24.483527339038474	24.640856080033693
13	23.177048305546307	13.783298677644765	28.391388043015688	34.64826497379324
14	21.316004754040165	20.404776972452588	33.936592217574514	24.342626055932737
15	29.59665629568542	18.545828197455837	29.316176746216723	22.541338760642017
16	31.853502356072315	18.77250506605161	28.793474881424626	20.580517696451448
17	35.334910663294444	18.178691051335257	24.055531422750153	22.430866862620146
18	32.774763014625954	19.36179877356348	23.81132458231259	24.05211362949798
19	28.03924508664657	22.816305740349144	25.837193969689686	23.307255203314597
20	29.890366012581886	25.722312015858563	22.08181094531751	22.305511026242037
21	27.56229755087551	21.466608159926263	29.984741206899972	20.986353082298255
22	27.56042327715657	18.69169079334699	26.950843312922125	26.797042616574313
23	28.295248826373896	24.257070973233166	25.576228917176948	21.87145128321599
24	29.486294649058785	24.234359185815496	23.0955725244703	23.18377364065542
25	26.682160662743186	22.390625103360684	20.73597216372773	30.1912420701684
26	29.397652527292735	21.631213493006754	24.045939551365024	24.92519442833549
27	23.390936012295235	19.176245675389023	22.357990690372187	35.074827621943555
28	24.790908228943636	27.06737903768172	21.495053019895966	26.646659713478677
29	31.574456074741626	21.588987208633124	22.255236390016517	24.581320326608733
30	26.87024954300797	24.075376673891807	25.183292944572212	23.871080838528012
31	34.117625008544486	20.82737057037457	22.41951096891131	22.635493452169637
32	33.71377414781184	19.57734025124088	25.43235084639996	21.27653475454732
33	37.05736821099471	18.900617187310505	23.05268473172528	20.989329869969506
34	27.099792947879752	21.841352652317816	28.204291425307986	22.854562974494442
35	23.98177323934034	30.85539647504239	25.339519171615116	19.82331111400215
36	28.11675181749425	28.327221730991003	22.59624395546726	20.959782496047488
37	29.69599280278892	25.91425969495644	23.56469221117993	20.825055291074708
38	30.91570398823397	27.701324560262307	21.171134420706096	20.211837030797625
39	29.93601009020769	23.967550809355494	21.097596740086747	24.99884236035007
40	26.18713189815417	21.946863237554272	23.780123437462102	28.085881426829456
41	23.761270448877532	25.85307017060301	24.589258427065396	25.796400953454068
42	23.08730366982794	23.51265355263071	25.499163191910196	27.900879585631156
43	23.09678528981784	21.134420706094037	24.395436474248584	31.37335752983954
44	24.311865916663177	20.29441532582595	23.05444875404898	32.3392700034619
45	29.262925322319955	22.4907333702308	22.3647160254813	25.881625281967946
46	25.77633519952195	24.71020420763425	22.41157286845465	27.10188772438915
47	26.481613374817258	22.457106694685223	25.55528115208298	25.50599877841454
48	27.40111001104719	21.110055147747893	26.585029183544318	24.903805657660598
49	29.911754783256782	21.161983554901887	24.465225607430064	24.46103605441127
50	23.535916597024535	22.216097144709366	24.49962404274226	29.748362215523837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	5.5
10	9.0
11	6.5
12	4.0
13	75.0
14	146.0
15	617.0
16	1088.0
17	1705.5
18	2323.0
19	2248.5
20	2174.0
21	2181.5
22	2189.0
23	2301.0
24	2413.0
25	2525.5
26	2638.0
27	3422.5
28	4207.0
29	5126.5
30	6046.0
31	6091.0
32	6136.0
33	6857.5
34	7579.0
35	9818.0
36	12057.0
37	12943.0
38	13829.0
39	15639.0
40	17449.0
41	19042.0
42	20635.0
43	21562.0
44	22489.0
45	23540.0
46	24591.0
47	27767.5
48	30944.0
49	32669.0
50	34394.0
51	37804.5
52	41215.0
53	42238.0
54	43261.0
55	45701.0
56	48141.0
57	58057.0
58	67973.0
59	74647.5
60	81322.0
61	79908.0
62	78494.0
63	76648.5
64	74803.0
65	88466.0
66	102129.0
67	79316.5
68	56504.0
69	42849.0
70	29194.0
71	25368.5
72	21543.0
73	19563.0
74	17583.0
75	14392.0
76	11201.0
77	11893.0
78	12585.0
79	8929.0
80	5273.0
81	3261.5
82	1250.0
83	1080.0
84	910.0
85	577.0
86	244.0
87	135.0
88	26.0
89	22.5
90	19.0
91	10.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	907018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.059399889326027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.21919776172042	7.876415878438592
2	11.583223250284702	2.5620699586447455
3	4.395902833250651	1.4584814192262066
4	2.263942816578496	1.001513957404347
5	1.2909673323078634	0.7138661986024551
6	0.781980211598206	0.5188939119362604
7	0.4879876512226001	0.37777954031465844
8	0.3579909408558707	0.3167331977344909
9	0.2799929146358759	0.2786898248222469
>10	3.020923553982075	8.725313932143182
>50	2.07794741647625	16.73178506216549
>100	2.1109465814154786	41.99203171989336
>500	0.08799777317127527	6.632707239569151
>1k	0.03799903841486887	6.497011836768795
>5k	9.99974695128128E-4	0.8529899281166631
>10k+	0.001999949390256256	3.463716394219355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	20301	2.2382135745927867	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGA	11019	1.21486012405487	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7713	0.8503690114198394	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3039	0.3350539901082448	No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3033	0.3343924817368564	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	2868	0.31620100152367425	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2828	0.311790945714418	No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2492	0.27474647691666537	No Hit
TTCCGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2164	0.23858401928076398	No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2051	0.22612561161961506	No Hit
TTCCGCGGGGTTCATAATTCTGAGGTCAGGAGGTGGAATCTAGAGATGGC	1899	0.20936739954444125	No Hit
TTCCGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1752	0.19316044444542446	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC	1679	0.18511209259353179	No Hit
TTCCGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1672	0.18434033282691192	No Hit
TTCCGCGAGGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGG	1649	0.18180455073658958	No Hit
TTCCGCGGGGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTAC	1586	0.17485871283701096	No Hit
TTCCGCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG	1534	0.16912564028497779	No Hit
TTCCGCGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTC	1511	0.16658985819465547	No Hit
TTCCGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT	1505	0.165928349823267	No Hit
TTCCGCGGGACCCCATACTCTATCCCTCTTCCTGCTCCCAACACATACTT	1472	0.1622900537806306	No Hit
TTCCGCGGGTGAATGTGGGAAGGACTGTAATCATAATAAGCTCACTACTT	1363	0.15027265170040727	No Hit
TTCCGCGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCAT	1351	0.1489496349576304	No Hit
TTCCGCGGGCTAACCATACGCCTTGCTTTGGGTGGCTGCACCAACCGGCC	1313	0.14476008193883694	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1305	0.14387807077698567	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1287	0.14189354566282036	No Hit
TTCCGCGGGGCTGATTCAAGACTAAGGGAGATGCTTTGGTTTTCTTAGAC	1231	0.1357194675298616	No Hit
TTCCGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	1212	0.13362469102046484	No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC	1193	0.13152991451106813	No Hit
TTCCGCGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1175	0.12954538939690283	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1122	0.12370206544963826	No Hit
TTCCGCGGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAA	1094	0.12061502638315888	No Hit
TTCCGCAGGTACAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATT	1085	0.11962276382607621	No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1073	0.11829974708329934	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGG	1051	0.11587421638820838	No Hit
TTCCGCGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGC	1042	0.11488195383112573	No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	1039	0.11455119964543152	No Hit
TTCCGCGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGC	1036	0.1142204454597373	No Hit
TTCCGCGGGCGTCTTTAAAATAAAGGGGAGGGGAGGGGGAGATGATGCAT	1020	0.1124564231360348	No Hit
TTCCGCGGGGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCA	1018	0.11223592034557198	No Hit
TTCCGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1003	0.11058214941710087	No Hit
TTCCGCGGGGGGCAGGCAGCTTTTACATCCTGTAGTGCCTGATAGCACCA	1001	0.11036164662663805	No Hit
TTCCGCGGGGAGCCTTTGCTGAGGAGCTGGGCTTCCACAGGGACTCAAGT	976	0.10760536174585289	No Hit
TTCCGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT	960	0.10584133942215038	No Hit
TTCCGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	954	0.10517983105076195	No Hit
TTCCGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	923	0.10176203779858833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.3075418569422E-4	0.0	0.0	0.0	0.0
8	6.6150837138844E-4	0.0	0.0	0.0	0.0
9	0.003197290461710793	0.0	0.0	0.0	0.0
10	0.01907349137503335	0.0	0.0	0.0	0.0
11	0.04939262506367018	0.0	0.0	0.0	0.0
12	0.05424368645385207	0.0	0.0	0.0	0.0
13	0.06019726179634803	0.0	0.0	0.0	0.0
14	0.1210560319640845	0.0	0.0	0.0	0.0
15	0.13164016590629954	0.0	0.0	0.0	0.0
16	0.1476266182148535	0.0	0.0	0.0	0.0
17	0.15302893658119243	0.0	0.0	0.0	0.0
18	0.15677748401906025	0.0	0.0	0.0	0.0
19	0.17022815423729187	0.0	0.0	0.0	0.0
20	0.17970977422719284	0.0	0.0	0.0	0.0
21	0.1870966177076971	0.0	0.0	0.0	0.0
22	0.19305019305019305	0.0	0.0	0.0	0.0
23	0.20583935489703623	0.0	0.0	0.0	0.0
24	0.2268973713862349	0.0	0.0	0.0	0.0
25	0.23296119812396227	0.0	0.0	0.0	0.0
26	0.2437658348566401	0.0	0.0	0.0	0.0
27	0.2534679576370039	0.0	0.0	0.0	0.0
28	0.3576555261306832	0.0	0.0	0.0	0.0
29	0.36537312379688164	0.0	0.0	0.0	0.0
30	0.39690502283306395	0.0	0.0	0.0	0.0
31	0.40550463166111367	0.0	0.0	0.0	0.0
32	0.41377348630346916	0.0	0.0	0.0	0.0
33	0.42171158676013043	0.0	0.0	0.0	0.0
34	0.4310829553548	0.0	0.0	0.0	0.0
35	0.5445316410479175	0.0	0.0	0.0	0.0
36	0.559305328008926	0.0	0.0	0.0	0.0
37	0.5715432328796121	0.0	0.0	0.0	0.0
38	0.5803633444981247	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.855601E-4	44.000004	29
AACGTTG	20	7.855601E-4	44.000004	29
CGGAATT	40	8.309144E-9	44.000004	32
TAATAGG	65	0.0	44.000004	13
AACCGAA	20	7.855601E-4	44.000004	35
GATAATC	40	8.309144E-9	44.000004	13
CGTATAG	20	7.855601E-4	44.000004	26
CGTATAC	20	7.855601E-4	44.000004	18
GTACACG	40	8.309144E-9	44.000004	32
TTTACGC	20	7.855601E-4	44.000004	13
ACGCATA	20	7.855601E-4	44.000004	26
TCATTCG	20	7.855601E-4	44.000004	43
ACGCAAT	20	7.855601E-4	44.000004	18
ACGCAAC	20	7.855601E-4	44.000004	15
ACACCTA	20	7.855601E-4	44.000004	40
CTATACC	20	7.855601E-4	44.000004	28
CGCATAA	20	7.855601E-4	44.000004	27
ATATGTG	20	7.855601E-4	44.000004	12
GTAGGGT	20	7.855601E-4	44.000004	44
CGATTAT	20	7.855601E-4	44.000004	42
>>END_MODULE