FastQCFastQC Report
Fri 17 Jun 2016
SRR1527287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978770
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC193211.9740081939577225No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG106941.0925958090256138No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT76130.7778129693390684No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC29150.2978227775677636No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28200.28811671792147286No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT27490.2808627154489819No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26180.26747857004199144No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT23200.237032193467311No Hit
TTCCGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC22550.23039120528827No Hit
TTCCGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA21850.22323937186468729No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC18930.1934060095834568No Hit
TTCCGCGGGGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTACAT18290.1868671904533241No Hit
TTCCGCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC17780.18165656895899956No Hit
TTCCGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG16720.17082664977471723No Hit
TTCCGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT15830.16173360442187643No Hit
TTCCGCGAGGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGGAG15530.1586685329546267No Hit
TTCCGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT14530.14845162806379436No Hit
TTCCGCGGGACCCCATACTCTATCCCTCTTCCTGCTCCCAACACATACTTCC14080.1438540208629198No Hit
TTCCGCGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTCCC13730.14027810415112846No Hit
TTCCGCGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCATAA13440.13731520173278708No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC13410.1370086945860621No Hit
TTCCGCGGGGTTCATAATTCTGAGGTCAGGAGGTGGAATCTAGAGATGGCCT13270.13557832790134558No Hit
TTCCGCGGGCTAACCATACGCCTTGCTTTGGGTGGCTGCACCAACCGGCCTT13260.13547615885243725No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT13090.13373928502099572No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12930.13210458023846255No Hit
TTCCGCGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC11680.11933344912492208No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT11630.11882260388038048No Hit
TTCCGCGGGGCTGATTCAAGACTAAGGGAGATGCTTTGGTTTTCTTAGACTC11550.11800525148911388No Hit
TTCCGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC11390.11637054670658072No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT11350.11596187051094742No Hit
TTCCGCGGGTGAATGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGG11220.1146336728751392No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10910.11146643235898117No Hit
TTCCGCGGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG10610.10840136089173145No Hit
TTCCGCGGGGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAA10580.10809485374500649No Hit
TTCCGCGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG10480.10707316325592325No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG10300.10523412037557343No Hit
TTCCGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC10240.10462110608212348No Hit
TTCCGCAGGTACAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAA10050.10267989415286533No Hit
TTCCGCGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTC10030.1024755560550487No Hit
TTCCGCGGGGGGCAGGCAGCTTTTACATCCTGTAGTGCCTGATAGCACCAGT9880.10094302032142384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG206.3109916E-446.00000413
ATCTAGC206.3109916E-446.00000433
ATTACGA405.6097633E-946.00000437
CGTCTAT351.01936166E-746.00000421
CGATTGG351.01936166E-746.00000443
CGATTCG405.6097633E-946.00000434
TAACGGT206.3109916E-446.00000433
GCCGCTA206.3109916E-446.00000427
CTGAATC206.3109916E-446.00000414
CGTTAAT351.01936166E-746.00000434
TAACCGG206.3109916E-446.00000422
CTACGTA206.3109916E-446.00000419
AACATCG206.3109916E-446.00000445
CTAAACG351.01936166E-746.00000446
CGCTACA351.01936166E-746.00000433
ATCCCGA206.3109916E-446.00000428
TAGGGCC206.3109916E-446.00000446
CATACGC1400.046.00000415
ACTCGAC206.3109916E-446.00000427
TTTATCG206.3109916E-446.00000413
Produced by FastQC (version 0.11.5)