##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978770
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47217221614884	33.0	31.0	34.0	31.0	34.0
2	32.893104610889175	34.0	31.0	34.0	31.0	34.0
3	32.8291151138674	34.0	31.0	34.0	31.0	34.0
4	36.384845264975425	37.0	37.0	37.0	35.0	37.0
5	36.10082961267714	37.0	37.0	37.0	35.0	37.0
6	36.15243009082828	37.0	37.0	37.0	35.0	37.0
7	36.50152027544775	37.0	37.0	37.0	35.0	37.0
8	36.592955444077774	37.0	37.0	37.0	35.0	37.0
9	38.6762283273905	39.0	39.0	39.0	38.0	39.0
10	37.9334491249221	39.0	38.0	39.0	35.0	39.0
11	37.637537930259406	39.0	38.0	39.0	35.0	39.0
12	36.95135118567181	39.0	35.0	39.0	33.0	39.0
13	36.814629586113185	39.0	35.0	39.0	33.0	39.0
14	37.56129223413059	40.0	36.0	40.0	33.0	40.0
15	37.415309010288425	40.0	35.0	40.0	33.0	40.0
16	32.2275151465615	38.0	33.0	40.0	3.0	40.0
17	33.481986574986976	38.0	33.0	40.0	15.0	40.0
18	35.470452711055714	38.0	33.0	40.0	27.0	40.0
19	36.19389335594675	38.0	34.0	40.0	30.0	40.0
20	36.69648844978902	38.0	35.0	40.0	31.0	40.0
21	36.97634377841577	39.0	35.0	40.0	33.0	40.0
22	36.9941416267356	39.0	35.0	40.0	33.0	40.0
23	36.93560693523504	39.0	35.0	40.0	33.0	40.0
24	36.878060218437426	38.0	35.0	40.0	33.0	40.0
25	36.785195704813184	38.0	35.0	40.0	33.0	40.0
26	36.549829888533566	38.0	35.0	40.0	32.0	40.0
27	36.363079170796	38.0	35.0	40.0	32.0	40.0
28	36.19081602419363	37.0	35.0	40.0	31.0	40.0
29	35.90600651838532	37.0	35.0	40.0	31.0	40.0
30	35.71124063876089	37.0	34.0	40.0	31.0	40.0
31	35.55247095844785	36.0	34.0	40.0	30.0	40.0
32	35.15074123644983	35.0	34.0	40.0	30.0	40.0
33	34.91108432011606	35.0	34.0	39.0	29.0	40.0
34	34.42605719423358	35.0	33.0	39.0	26.0	40.0
35	34.43757777618848	35.0	33.0	39.0	27.0	40.0
36	34.46009072611543	35.0	33.0	39.0	28.0	40.0
37	34.110455980465275	35.0	33.0	39.0	27.0	40.0
38	34.06563544039969	35.0	33.0	38.0	27.0	40.0
39	33.699801792045115	35.0	33.0	38.0	26.0	40.0
40	33.60358613361668	35.0	33.0	38.0	26.0	40.0
41	33.67396936971914	35.0	33.0	37.0	26.0	40.0
42	33.58642888523351	35.0	33.0	37.0	26.0	40.0
43	33.27830235908334	35.0	33.0	37.0	25.0	40.0
44	33.62933886408452	35.0	33.0	37.0	27.0	40.0
45	33.65093535764276	35.0	33.0	37.0	27.0	40.0
46	33.56491923536684	35.0	33.0	36.0	26.0	40.0
47	33.30200762181105	35.0	33.0	36.0	26.0	39.0
48	33.19784423306803	35.0	33.0	36.0	25.0	39.0
49	33.197746150781086	35.0	33.0	36.0	26.0	39.0
50	32.63889371353842	35.0	33.0	35.0	24.0	39.0
51	32.7624140502876	35.0	33.0	35.0	24.0	38.0
52	32.42690928410148	35.0	33.0	35.0	24.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	4.0
15	17.0
16	33.0
17	90.0
18	211.0
19	410.0
20	836.0
21	1431.0
22	2383.0
23	3503.0
24	5083.0
25	7538.0
26	10431.0
27	13855.0
28	17390.0
29	21282.0
30	24934.0
31	31244.0
32	42156.0
33	67868.0
34	116274.0
35	151133.0
36	153543.0
37	147362.0
38	128183.0
39	31573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.4538042645361	0.39498554307957945	0.06886193896421018	0.08234825342010892
8	99.23087139981814	0.5786854930167455	0.054660441165953186	0.13578266599916222
9	98.53458933150792	0.7631006262962697	0.3437988495765093	0.3585111926193079
10	54.39531248403608	34.611808698672824	3.163358092299519	7.82952072499157
11	37.426157319901506	23.820100738682225	20.82154132227183	17.932200619144435
12	38.15911807676982	12.778998130306404	24.355670893059656	24.706212899864116
13	23.348386239872493	13.589607364345046	28.307058859589073	34.754947536193384
14	21.530798859793414	20.285766829796582	33.75266916640273	24.430765144007275
15	29.968736271034054	18.511090450258997	29.142290834414624	22.377882444292325
16	30.143445344667285	26.570900211489935	25.39227806328351	17.89337638055927
17	35.62992327104427	17.906556187868446	24.103313342256097	22.360207198831187
18	33.091737589014784	19.34284867742166	23.58613361668216	23.97928011688139
19	28.236868722989055	22.76724868968195	25.792985073101953	23.20289751422704
20	29.71658305832831	25.93050461293256	22.1112212266416	22.24169110209753
21	27.684747182688476	21.455602439796888	29.89394852723316	20.965701850281476
22	27.23908579135037	18.52549628615507	27.05722488429355	27.17819303820101
23	28.461129785342827	24.284050389774922	25.390541189452065	21.864278635430182
24	29.595921411567577	24.19700236010503	23.014599957089	23.19247627123839
25	26.81549291457646	22.214003289843376	20.607190657662166	30.363313137917995
26	29.560264413498576	21.413304453548843	24.014528438754766	25.01190269419782
27	23.433595226662035	19.062292469119406	22.209814358838134	35.29429794538043
28	24.90881412384932	27.13855144722458	21.30541393790165	26.64722049102445
29	32.0424614567263	21.42760812039601	21.995157187081745	24.53477323579595
30	26.96476189503152	24.055702565464816	25.1405335267734	23.839002012730266
31	34.381315324335645	20.618429253042084	22.406591947035565	22.593663475586705
32	33.8810956608805	19.103568764878368	25.64392043074471	21.37141514349643
33	37.46906832044301	18.75108554614465	22.944205482391165	20.83564065102118
34	27.172471571462143	21.691510773726208	28.014855379711268	23.12116227510038
35	23.90745527549884	30.923097356886704	25.345484638883498	19.823962728730958
36	28.322690724072046	28.179041041306945	22.53726616058931	20.961002074031693
37	30.168783268796552	25.710330312535117	23.430836662341513	20.690049756326818
38	30.96345413120549	27.484393677779252	21.08677217323784	20.465380017777417
39	30.358000347374762	23.84768638188747	20.840851272515504	24.95346199822226
40	26.442473717012167	21.81503315385637	23.75461037833199	27.987882750799475
41	23.796193181237676	25.758247596473122	24.668819027963668	25.77674019432553
42	23.0329903858925	23.320800596667247	25.61173718033859	28.03447183710167
43	23.097152548606925	20.949150464358326	24.408083615149625	31.54561337188512
44	24.26729466575396	20.110751249016623	22.914780796305568	32.707173288923855
45	29.3138326675317	22.47381918121724	22.18008316560581	26.03226498564525
46	25.469313526160388	24.571145417207312	22.360207198831187	27.599333857801117
47	26.948517016255096	22.473001828825975	25.276316192772562	25.302164962146367
48	27.536193385575775	21.035789817832587	26.61871532637903	24.809301470212613
49	30.08970442494151	21.030987872533892	24.536305771529573	24.343001930995026
50	23.68554410126996	22.20460373734381	24.34341060719066	29.766441554195573
51	21.995872370424106	25.05685707571748	26.40773624038334	26.539534313475077
52	25.356416727116688	22.734963270226917	26.32211857739816	25.586501425258234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	5.0
10	8.0
11	6.0
12	4.0
13	88.5
14	611.5
15	1050.0
16	1739.0
17	2428.0
18	2329.5
19	2231.0
20	2186.5
21	2142.0
22	2193.5
23	2245.0
24	2354.5
25	2464.0
26	2881.5
27	3299.0
28	4286.0
29	5273.0
30	5776.0
31	6279.0
32	6529.0
33	6779.0
34	8717.0
35	10655.0
36	10795.5
37	10936.0
38	14093.0
39	18072.0
40	18894.0
41	19252.0
42	19610.0
43	20378.0
44	21146.0
45	25015.5
46	28885.0
47	30656.5
48	32428.0
49	34564.5
50	36701.0
51	39211.5
52	41722.0
53	44380.0
54	47038.0
55	50537.0
56	54036.0
57	59367.0
58	64698.0
59	76325.0
60	87952.0
61	86803.5
62	85655.0
63	85260.5
64	94776.5
65	104687.0
66	82510.0
67	60333.0
68	47117.0
69	33901.0
70	28664.0
71	23427.0
72	21316.5
73	19206.0
74	17429.5
75	15653.0
76	15203.5
77	14754.0
78	10561.0
79	6368.0
80	4473.5
81	2579.0
82	1571.5
83	564.0
84	440.5
85	317.0
86	197.5
87	78.0
88	50.5
89	16.5
90	10.0
91	9.5
92	9.0
93	8.5
94	8.0
95	6.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	978770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.90143790000988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43419919053494	9.216038847936225
2	10.188982112131995	2.629050399679649
3	4.156768747181571	1.6088488156939473
4	2.238715870874853	1.1553061513543383
5	1.404822529121863	0.9062115310000267
6	0.9916503912313664	0.7676229564595185
7	0.7066333061591966	0.638161000224408
8	0.584561011126946	0.6033342067057023
9	0.4473828777976536	0.5194694173890724
>10	4.33503448606098	12.686517435884845
>50	1.7927069784343195	16.69655306807116
>100	1.6156691896387594	36.850343280570584
>500	0.07177207653874056	6.1363567242548145
>1k	0.028708830615496218	5.714837794971044
>5k	7.974675170971171E-4	0.7832618034262513
>10k+	0.0015949350341942343	3.088086566378423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	19321	1.9740081939577225	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG	10694	1.0925958090256138	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7613	0.7778129693390684	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2915	0.2978227775677636	No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2820	0.28811671792147286	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	2749	0.2808627154489819	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2618	0.26747857004199144	No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2320	0.237032193467311	No Hit
TTCCGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2255	0.23039120528827	No Hit
TTCCGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2185	0.22323937186468729	No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1893	0.1934060095834568	No Hit
TTCCGCGGGGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTACAT	1829	0.1868671904533241	No Hit
TTCCGCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1778	0.18165656895899956	No Hit
TTCCGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1672	0.17082664977471723	No Hit
TTCCGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1583	0.16173360442187643	No Hit
TTCCGCGAGGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGGAG	1553	0.1586685329546267	No Hit
TTCCGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT	1453	0.14845162806379436	No Hit
TTCCGCGGGACCCCATACTCTATCCCTCTTCCTGCTCCCAACACATACTTCC	1408	0.1438540208629198	No Hit
TTCCGCGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTCCC	1373	0.14027810415112846	No Hit
TTCCGCGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCATAA	1344	0.13731520173278708	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC	1341	0.1370086945860621	No Hit
TTCCGCGGGGTTCATAATTCTGAGGTCAGGAGGTGGAATCTAGAGATGGCCT	1327	0.13557832790134558	No Hit
TTCCGCGGGCTAACCATACGCCTTGCTTTGGGTGGCTGCACCAACCGGCCTT	1326	0.13547615885243725	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1309	0.13373928502099572	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1293	0.13210458023846255	No Hit
TTCCGCGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1168	0.11933344912492208	No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT	1163	0.11882260388038048	No Hit
TTCCGCGGGGCTGATTCAAGACTAAGGGAGATGCTTTGGTTTTCTTAGACTC	1155	0.11800525148911388	No Hit
TTCCGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	1139	0.11637054670658072	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1135	0.11596187051094742	No Hit
TTCCGCGGGTGAATGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGG	1122	0.1146336728751392	No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1091	0.11146643235898117	No Hit
TTCCGCGGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG	1061	0.10840136089173145	No Hit
TTCCGCGGGGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAA	1058	0.10809485374500649	No Hit
TTCCGCGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG	1048	0.10707316325592325	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG	1030	0.10523412037557343	No Hit
TTCCGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC	1024	0.10462110608212348	No Hit
TTCCGCAGGTACAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAA	1005	0.10267989415286533	No Hit
TTCCGCGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTC	1003	0.1024755560550487	No Hit
TTCCGCGGGGGGCAGGCAGCTTTTACATCCTGTAGTGCCTGATAGCACCAGT	988	0.10094302032142384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.065071467249711E-4	0.0	0.0	0.0	0.0
8	6.130142934499422E-4	0.0	0.0	0.0	0.0
9	0.0034737476628830064	0.0	0.0	0.0	0.0
10	0.019718626439306476	0.0	0.0	0.0	0.0
11	0.04720210059564556	0.0	0.0	0.0	0.0
12	0.05159536969870347	0.0	0.0	0.0	0.0
13	0.0573168364375696	0.0	0.0	0.0	0.0
14	0.1111599252122562	0.0	0.0	0.0	0.0
15	0.11974212532055539	0.0	0.0	0.0	0.0
16	0.14017593510222012	0.0	0.0	0.0	0.0
17	0.14640824708562789	0.0	0.0	0.0	0.0
18	0.15223188287340234	0.0	0.0	0.0	1.0216904890832371E-4
19	0.16868109974764245	0.0	0.0	0.0	1.0216904890832371E-4
20	0.18043054037209968	0.0	0.0	0.0	1.0216904890832371E-4
21	0.18717369760004904	0.0	0.0	0.0	1.0216904890832371E-4
22	0.19238431909437356	0.0	0.0	0.0	1.0216904890832371E-4
23	0.20331640732756417	0.0	0.0	0.0	1.0216904890832371E-4
24	0.21966345515289598	0.0	0.0	0.0	1.0216904890832371E-4
25	0.22558925998957877	0.0	0.0	0.0	1.0216904890832371E-4
26	0.2343757981956946	0.0	0.0	0.0	1.0216904890832371E-4
27	0.24316233640181042	0.0	0.0	0.0	1.0216904890832371E-4
28	0.35166586634245023	0.0	0.0	0.0	1.0216904890832371E-4
29	0.3592263759616662	0.0	0.0	0.0	1.0216904890832371E-4
30	0.3952920502263044	0.0	0.0	0.0	1.0216904890832371E-4
31	0.40428292653023695	0.0	0.0	0.0	1.0216904890832371E-4
32	0.4123542813939945	0.0	0.0	0.0	1.0216904890832371E-4
33	0.41950611481757716	0.0	0.0	0.0	1.0216904890832371E-4
34	0.4288034982682346	0.0	0.0	0.0	1.0216904890832371E-4
35	0.537817873453416	0.0	0.0	0.0	1.0216904890832371E-4
36	0.5549822736700144	0.0	0.0	0.0	1.0216904890832371E-4
37	0.5651991785608468	0.0	0.0	0.0	1.0216904890832371E-4
38	0.5730661953267877	0.0	0.0	0.0	1.0216904890832371E-4
39	0.5818527335329036	0.0	0.0	0.0	1.0216904890832371E-4
40	0.5901284264944777	0.0	0.0	0.0	1.0216904890832371E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	6.3109916E-4	46.000004	13
ATCTAGC	20	6.3109916E-4	46.000004	33
ATTACGA	40	5.6097633E-9	46.000004	37
CGTCTAT	35	1.01936166E-7	46.000004	21
CGATTGG	35	1.01936166E-7	46.000004	43
CGATTCG	40	5.6097633E-9	46.000004	34
TAACGGT	20	6.3109916E-4	46.000004	33
GCCGCTA	20	6.3109916E-4	46.000004	27
CTGAATC	20	6.3109916E-4	46.000004	14
CGTTAAT	35	1.01936166E-7	46.000004	34
TAACCGG	20	6.3109916E-4	46.000004	22
CTACGTA	20	6.3109916E-4	46.000004	19
AACATCG	20	6.3109916E-4	46.000004	45
CTAAACG	35	1.01936166E-7	46.000004	46
CGCTACA	35	1.01936166E-7	46.000004	33
ATCCCGA	20	6.3109916E-4	46.000004	28
TAGGGCC	20	6.3109916E-4	46.000004	46
CATACGC	140	0.0	46.000004	15
ACTCGAC	20	6.3109916E-4	46.000004	27
TTTATCG	20	6.3109916E-4	46.000004	13
>>END_MODULE