FastQCFastQC Report
Fri 17 Jun 2016
SRR1527286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947045
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC182961.9319039749959084No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG108691.147675136873116No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT73640.7775765671113833No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28100.2967124054295202No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT27760.2931222909154264No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26900.2840414130268361No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC26020.2747493519315344No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24740.2612336267020047No Hit
TTCCGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA19760.2086490082308655No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC18790.19840662270536247No Hit
TTCCGCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC18090.19101521047046338No Hit
TTCCGCGAGGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGGAG16440.1735925959167727No Hit
TTCCGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG16350.1726422714865714No Hit
TTCCGCGGGGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTACAT16100.17000248140267885No Hit
TTCCGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT14730.1555364317429478No Hit
TTCCGCGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTCCC14610.15426933250267938No Hit
TTCCGCGGGACCCCATACTCTATCCCTCTTCCTGCTCCCAACACATACTTCC14260.15057362638522984No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC13350.14096479047986105No Hit
TTCCGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT13330.14075360727314964No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12870.13589639351878738No Hit
TTCCGCGGGGTTCATAATTCTGAGGTCAGGAGGTGGAATCTAGAGATGGCCT12080.127554656853687No Hit
TTCCGCGGGCTAACCATACGCCTTGCTTTGGGTGGCTGCACCAACCGGCCTT12000.1267099240268414No Hit
TTCCGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC11990.12660433242348568No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT11660.12311980951274755No Hit
TTCCGCGGGTGAATGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGG11610.12259185149596905No Hit
TTCCGCGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCATAA11350.11984646980872081No Hit
TTCCGCGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC10990.11604517208791557No Hit
TTCCGCGGGGCTGATTCAAGACTAAGGGAGATGCTTTGGTTTTCTTAGACTC10970.11583398888120418No Hit
TTCCGCGGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG10840.11446129803758004No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT10780.11382774841744583No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT10720.11319419879731163No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10670.11266624078053313No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG10530.11118795833355331No Hit
TTCCGCGGGGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAA9890.10443009571878845No Hit
TTCCGCGGGCGTCTTTAAAATAAAGGGGAGGGGAGGGGGAGATGATGCATTG9830.10379654609865424No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA9720.10263503846174153No Hit
TTCCGCAGGTACAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAA9670.10210708044496301No Hit
TTCCGCGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTC9630.10168471403154021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA351.0193071E-746.00000412
GCGATTT351.0193071E-746.0000049
AACTACG351.0193071E-746.00000419
TCGAACG351.0193071E-746.00000426
CCCGCGA351.0193071E-746.00000446
AATCGGT351.0193071E-746.00000442
ATCTCGA253.4166962E-546.046
AATCATA2050.046.029
CGAGTCA206.3108886E-446.046
AGATACG501.6370905E-1146.041
CGAAACT253.4166962E-546.010
ACGCATT206.3108886E-446.016
ACGCATA453.092282E-1046.026
AACCCGC206.3108886E-446.035
ACGCACC253.4166962E-546.014
AACTTAC253.4166962E-546.040
CGGTTAT206.3108886E-446.042
TTATGCG206.3108886E-446.015
TAGAACG301.8610535E-646.024
TCTTACG301.8610535E-646.018
Produced by FastQC (version 0.11.5)