##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	947045
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.443760328178705	33.0	31.0	34.0	31.0	34.0
2	32.8697200238637	34.0	31.0	34.0	31.0	34.0
3	32.80475478989911	34.0	31.0	34.0	31.0	34.0
4	36.38761093717827	37.0	37.0	37.0	35.0	37.0
5	36.11276021730752	37.0	37.0	37.0	35.0	37.0
6	36.157539504458605	37.0	37.0	37.0	35.0	37.0
7	36.5155161581551	37.0	37.0	37.0	35.0	37.0
8	36.62315412678384	37.0	37.0	37.0	35.0	37.0
9	38.62051539261598	39.0	39.0	39.0	38.0	39.0
10	37.92198364386064	39.0	38.0	39.0	35.0	39.0
11	37.56252448405303	39.0	37.0	39.0	35.0	39.0
12	36.915442244032754	39.0	35.0	39.0	33.0	39.0
13	36.724250695584686	39.0	35.0	39.0	33.0	39.0
14	37.48292108611523	40.0	36.0	40.0	33.0	40.0
15	37.533601887977866	40.0	36.0	40.0	33.0	40.0
16	37.3850281665602	39.0	35.0	40.0	33.0	40.0
17	37.266654699618286	39.0	35.0	40.0	33.0	40.0
18	37.29954437223152	39.0	35.0	40.0	33.0	40.0
19	36.90581123389068	39.0	35.0	40.0	32.0	40.0
20	36.991233785089406	39.0	35.0	40.0	32.0	40.0
21	32.73758374734041	38.0	33.0	40.0	10.0	40.0
22	33.233959315555225	37.0	33.0	40.0	15.0	40.0
23	35.23969293961744	37.0	33.0	40.0	27.0	40.0
24	36.12538897306886	37.0	35.0	40.0	31.0	40.0
25	36.4575928282183	38.0	35.0	40.0	32.0	40.0
26	36.41922717505504	38.0	35.0	40.0	32.0	40.0
27	36.32836243261936	38.0	35.0	40.0	32.0	40.0
28	36.16385810600341	37.0	35.0	40.0	31.0	40.0
29	36.05284965339556	37.0	35.0	40.0	31.0	40.0
30	35.79865265114118	37.0	35.0	40.0	31.0	40.0
31	35.54463515461251	36.0	34.0	40.0	30.0	40.0
32	35.157982989192696	35.0	34.0	40.0	30.0	40.0
33	34.938461213564295	35.0	34.0	39.0	29.0	40.0
34	34.43707004419009	35.0	33.0	39.0	26.0	40.0
35	34.464472121176925	35.0	33.0	39.0	27.0	40.0
36	34.41976041265199	35.0	33.0	39.0	28.0	40.0
37	34.26348272785348	35.0	33.0	39.0	28.0	40.0
38	34.17079019476371	35.0	33.0	38.0	28.0	40.0
39	33.994142833761856	35.0	33.0	38.0	27.0	40.0
40	29.389454566572866	35.0	27.0	37.0	8.0	40.0
41	30.184698720757726	35.0	27.0	37.0	11.0	40.0
42	31.819194441658	35.0	28.0	36.0	21.0	40.0
43	32.464594607436815	35.0	31.0	36.0	23.0	39.0
44	33.302965540180246	35.0	33.0	37.0	26.0	40.0
45	33.516451699760836	35.0	33.0	37.0	26.0	40.0
46	33.53562502309816	35.0	33.0	36.0	26.0	39.0
47	33.50378493102229	35.0	33.0	36.0	27.0	39.0
48	33.31225865719158	35.0	33.0	36.0	26.0	39.0
49	33.25320866484697	35.0	33.0	36.0	26.0	39.0
50	33.0589929728788	35.0	33.0	35.0	26.0	39.0
51	32.948703599089804	35.0	33.0	35.0	26.0	39.0
52	32.55261260024603	35.0	33.0	35.0	24.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	3.0
15	24.0
16	28.0
17	96.0
18	213.0
19	444.0
20	877.0
21	1523.0
22	2499.0
23	3847.0
24	5504.0
25	7890.0
26	10622.0
27	13854.0
28	17795.0
29	22297.0
30	26573.0
31	35185.0
32	53462.0
33	64568.0
34	112529.0
35	145925.0
36	137035.0
37	125764.0
38	125970.0
39	32515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.45715356714834	0.38657085988522194	0.06831776737113865	0.08795780559529906
8	99.24047959706243	0.5898346963449467	0.03547877872751559	0.13420692786509617
9	98.43819459476583	0.8002787618328591	0.3935399057066982	0.3679867376946185
10	54.23670469724248	34.74322761853978	3.1856986732415034	7.834369010976247
11	37.41342808419875	23.91459751120591	20.8066142580342	17.865360146561144
12	38.25235337285979	12.748919005960646	24.399790928625357	24.598936692554208
13	23.474808483229413	13.602732710694845	28.184933134117173	34.737525671958565
14	21.509537561573104	20.347396375040258	33.77241841728746	24.370647646099183
15	29.975555543823155	18.333236541030256	29.190376381270163	22.500831533876426
16	31.632815758490885	18.549382553099377	29.065039148086946	20.752762540322795
17	35.76482638100618	17.84561451673363	24.056829400926038	22.33272970133415
18	33.17244692702037	19.265821581867808	23.588002682026726	23.973728809085102
19	28.282499775617843	22.755518481170377	25.826861447977656	23.135120295234124
20	29.646109741353367	25.96698150563067	22.063682295983824	22.323226457032135
21	32.39413121868549	20.590045879551656	27.945662560913153	19.070160340849696
22	27.333125669846737	18.404088506881934	26.96545570696218	27.297330116309148
23	28.438775348584283	24.296627932146837	25.422973565142097	21.841623154126783
24	29.694365104086923	24.293037817632744	22.981801287161645	23.030795791118692
25	26.80495646986152	22.174764662714022	20.58603339862414	30.43424546880032
26	29.591941248831894	21.466984145420756	23.950709839553557	24.990364766193792
27	23.384422070756933	19.042917707183925	22.24793964383952	35.32472057821962
28	24.91845688430856	27.204409505356136	21.22095570960197	26.656177900733336
29	31.95423659910564	21.427492885765723	21.993886246165705	24.62438426896293
30	26.75817938957494	24.214160889926035	25.07927289621929	23.948386824279734
31	34.432682713070655	20.584766299383872	22.440644319963678	22.54190666758179
32	33.842425650312286	19.007966886473188	25.711977783526653	21.437629679687873
33	37.46991959199404	18.82170329815373	22.892470790722722	20.815906319129503
34	27.039475420914528	21.705409985797928	28.01408591988765	23.241028673399892
35	23.85536062172336	31.049633333157345	25.26300228605821	19.832003759061077
36	28.47024164638428	28.131609374422545	22.490272373540858	20.907876605652316
37	30.01842573478557	25.799407631105176	23.507013922252902	20.675152711856352
38	31.14730556626137	27.389300402831967	20.988020632599298	20.47537339830737
39	30.02307176533322	23.903615984456916	20.87714944907581	25.196162801134054
40	26.95077847409574	26.370763796862875	21.865698039691882	24.8127596893495
41	23.68873707162806	25.791171486043428	24.681403734775014	25.838687707553493
42	22.9745154665301	23.351477490509957	25.720108336985042	27.9538987059749
43	23.090666230221373	20.975032865386545	24.332001119270995	31.602299785121087
44	24.277727035146164	20.131567137781204	22.82130205006098	32.769403777011654
45	29.41000691625002	22.47960762160193	22.227877239201938	25.88250822294611
46	25.317487553389757	24.60263239867166	22.368947621285155	27.710932426653436
47	26.87834263419373	22.458278117724078	25.246529995934726	25.416849252147465
48	27.599322101906452	21.087065556546943	26.565580305054144	24.74803203649246
49	30.2347829300614	21.045251281618086	24.448996615789113	24.2709691725314
50	23.519896097862297	22.25712611333147	24.344988886483744	29.87798890232249
51	22.065582944844227	25.269760148672976	26.291886869156166	26.37277003732663
52	25.492452840150154	22.79596006525561	26.22525856743872	25.48632852715552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	5.0
10	9.0
11	7.5
12	6.0
13	68.5
14	540.5
15	950.0
16	1573.5
17	2197.0
18	2218.5
19	2240.0
20	2220.5
21	2201.0
22	2166.5
23	2132.0
24	2262.0
25	2392.0
26	2798.5
27	3205.0
28	4184.5
29	5164.0
30	5488.5
31	5813.0
32	6203.5
33	6594.0
34	8414.0
35	10234.0
36	10287.5
37	10341.0
38	13323.5
39	17192.5
40	18079.0
41	18626.5
42	19174.0
43	19725.0
44	20276.0
45	23962.0
46	27648.0
47	29373.5
48	31099.0
49	33270.5
50	35442.0
51	37812.5
52	40183.0
53	42725.0
54	45267.0
55	48519.0
56	51771.0
57	56973.0
58	62175.0
59	73300.5
60	84426.0
61	84224.5
62	84023.0
63	81825.0
64	90935.5
65	102244.0
66	81392.0
67	60540.0
68	47108.5
69	33677.0
70	28485.5
71	23294.0
72	21183.5
73	19073.0
74	16837.0
75	14601.0
76	14483.5
77	14366.0
78	10489.0
79	6612.0
80	4598.0
81	2584.0
82	1561.0
83	538.0
84	416.0
85	294.0
86	186.0
87	78.0
88	47.0
89	13.5
90	11.0
91	8.0
92	5.0
93	5.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	947045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.315954426588878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.33428911008508	8.966203251366526
2	11.53876375575452	3.072993046216054
3	5.591316805164692	2.233611592865807
4	3.171450409122641	1.6892355645625496
5	1.932769064312597	1.2868332388753685
6	1.2848683504124425	1.0265549038955106
7	0.8810451501611101	0.8212369947118747
8	0.6678589397591861	0.711454336417863
9	0.47505842832226647	0.5693270743355614
>10	3.7825491407939884	11.743925208261121
>50	1.6789633868422174	16.21185788509802
>100	1.5634533088714833	36.263833803058745
>500	0.07077025903840742	6.372611534169541
>1k	0.024403537599450832	5.073544187669416
>5k	8.134512533150278E-4	0.7976596297529319
>10k+	0.0016269025066300556	3.15911774874311
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	18296	1.9319039749959084	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG	10869	1.147675136873116	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7364	0.7775765671113833	No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2810	0.2967124054295202	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	2776	0.2931222909154264	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2690	0.2840414130268361	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2602	0.2747493519315344	No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2474	0.2612336267020047	No Hit
TTCCGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1976	0.2086490082308655	No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1879	0.19840662270536247	No Hit
TTCCGCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1809	0.19101521047046338	No Hit
TTCCGCGAGGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGGAG	1644	0.1735925959167727	No Hit
TTCCGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1635	0.1726422714865714	No Hit
TTCCGCGGGGGCTGGAGACAGTGGAGTCCGTCACGCTGGCGGTGGTGTACAT	1610	0.17000248140267885	No Hit
TTCCGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1473	0.1555364317429478	No Hit
TTCCGCGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTCCC	1461	0.15426933250267938	No Hit
TTCCGCGGGACCCCATACTCTATCCCTCTTCCTGCTCCCAACACATACTTCC	1426	0.15057362638522984	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC	1335	0.14096479047986105	No Hit
TTCCGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT	1333	0.14075360727314964	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1287	0.13589639351878738	No Hit
TTCCGCGGGGTTCATAATTCTGAGGTCAGGAGGTGGAATCTAGAGATGGCCT	1208	0.127554656853687	No Hit
TTCCGCGGGCTAACCATACGCCTTGCTTTGGGTGGCTGCACCAACCGGCCTT	1200	0.1267099240268414	No Hit
TTCCGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	1199	0.12660433242348568	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1166	0.12311980951274755	No Hit
TTCCGCGGGTGAATGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGG	1161	0.12259185149596905	No Hit
TTCCGCGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCATAA	1135	0.11984646980872081	No Hit
TTCCGCGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1099	0.11604517208791557	No Hit
TTCCGCGGGGCTGATTCAAGACTAAGGGAGATGCTTTGGTTTTCTTAGACTC	1097	0.11583398888120418	No Hit
TTCCGCGGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG	1084	0.11446129803758004	No Hit
TTCCGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT	1078	0.11382774841744583	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1072	0.11319419879731163	No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1067	0.11266624078053313	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG	1053	0.11118795833355331	No Hit
TTCCGCGGGGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAA	989	0.10443009571878845	No Hit
TTCCGCGGGCGTCTTTAAAATAAAGGGGAGGGGAGGGGGAGATGATGCATTG	983	0.10379654609865424	No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	972	0.10263503846174153	No Hit
TTCCGCAGGTACAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAA	967	0.10210708044496301	No Hit
TTCCGCGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTC	963	0.10168471403154021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.2236641342280465E-4	0.0	0.0	0.0	0.0
8	7.391412234899081E-4	0.0	0.0	0.0	0.0
9	0.0026397900838925287	0.0	0.0	0.0	0.0
10	0.019640038224160414	0.0	0.0	0.0	0.0
11	0.04709385509664271	0.0	0.0	0.0	0.0
12	0.052373435264427774	0.0	0.0	0.0	0.0
13	0.058814523069125546	0.0	0.0	0.0	0.0
14	0.10685870259596957	0.0	0.0	0.0	0.0
15	0.11593958048455986	0.0	0.0	0.0	0.0
16	0.13431251946845188	0.0	0.0	0.0	0.0
17	0.14096479047986105	0.0	0.0	0.0	0.0
18	0.1450828630107334	0.0	0.0	0.0	0.0
19	0.15807063022348464	0.0	0.0	0.0	0.0
20	0.17105839743623588	0.0	0.0	0.0	0.0
21	0.17813303486106785	0.0	0.0	0.0	0.0
22	0.1841517562523428	0.0	0.0	0.0	0.0
23	0.19682274865502694	0.0	0.0	0.0	0.0
24	0.21762429451610008	0.0	0.0	0.0	0.0
25	0.22469893194093205	0.0	0.0	0.0	0.0
26	0.23525809227650218	0.0	0.0	0.0	0.0
27	0.2458172526120723	0.0	0.0	0.0	0.0
28	0.35225358879461904	0.0	0.0	0.0	0.0
29	0.36006736744294093	0.0	0.0	0.0	0.0
30	0.3876267759187789	0.0	0.0	0.0	0.0
31	0.39438463853354383	0.0	0.0	0.0	0.0
32	0.4015648675617315	0.0	0.0	0.0	0.0
33	0.40800595536642925	0.0	0.0	0.0	0.0
34	0.41603091722146257	0.0	0.0	0.0	0.0
35	0.5055725968670971	0.0	0.0	0.0	0.0
36	0.5186659556832041	0.0	0.0	0.0	0.0
37	0.5280636083818615	0.0	0.0	0.0	0.0
38	0.5355606122201163	0.0	0.0	0.0	0.0
39	0.5437967572818609	0.0	0.0	0.0	0.0
40	0.5545671008241425	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCA	35	1.0193071E-7	46.000004	12
GCGATTT	35	1.0193071E-7	46.000004	9
AACTACG	35	1.0193071E-7	46.000004	19
TCGAACG	35	1.0193071E-7	46.000004	26
CCCGCGA	35	1.0193071E-7	46.000004	46
AATCGGT	35	1.0193071E-7	46.000004	42
ATCTCGA	25	3.4166962E-5	46.0	46
AATCATA	205	0.0	46.0	29
CGAGTCA	20	6.3108886E-4	46.0	46
AGATACG	50	1.6370905E-11	46.0	41
CGAAACT	25	3.4166962E-5	46.0	10
ACGCATT	20	6.3108886E-4	46.0	16
ACGCATA	45	3.092282E-10	46.0	26
AACCCGC	20	6.3108886E-4	46.0	35
ACGCACC	25	3.4166962E-5	46.0	14
AACTTAC	25	3.4166962E-5	46.0	40
CGGTTAT	20	6.3108886E-4	46.0	42
TTATGCG	20	6.3108886E-4	46.0	15
TAGAACG	30	1.8610535E-6	46.0	24
TCTTACG	30	1.8610535E-6	46.0	18
>>END_MODULE