FastQCFastQC Report
Fri 17 Jun 2016
SRR1527285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4910685
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA261850.5332249981418071No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC209580.4267836360915025No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT193640.39432380614924395No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC162520.3309517918579587No Hit
TATTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG148630.30266653226586515No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC139580.28423733145172214No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126060.2567055308984388No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA117430.23913160791213445No Hit
TATTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA111470.22699480825994744No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC108210.2203562232152948No Hit
TATTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA106170.2162020166229355No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG101850.20740487325088047No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGA96940.19740626816829016No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC87940.17907888614317555No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA83400.1698337400993955No Hit
TATTGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC81690.16635153751462373No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAA72680.14800379173170342No Hit
TATTGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC72180.14698560384141926No Hit
TATTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG71590.14578414213088398No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT67480.1374146376727483No Hit
TATTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG64810.13197751433863095No Hit
TATTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC62080.1264182084576795No Hit
TATTGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA59280.12071635627208832No Hit
TATTGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC58800.11973889589741553No Hit
TATTGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA57400.11688796980461992No Hit
TATTGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC56670.11540141548480506No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT56520.11509595911771983No Hit
TATTGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA56330.11470904771941186No Hit
TATTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT55200.11240794308736968No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG54790.11157302901733668No Hit
TATTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA53230.10839628279965016No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT53050.10802973515914786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.5294157E-644.036
CGAATTA254.444976E-544.038
GTGGGAT321300.040.0423285
GTGGGGG828650.039.9326635
GTGGGGC660300.039.52875
TGGGGCT327350.039.4770136
TGGGATT125050.039.4082376
TGTGGGG2928000.039.346044
TACGTTA452.3535904E-839.1111131
CGATTAA850.038.82353230
TGGGGGT167700.038.6738246
TGGGGGC235100.038.5725256
TGGGGTC124450.038.5375676
GTGGGAC231550.038.3562935
TGGGAGA150550.038.213226
GTGGGGT546500.038.1266255
GTGGGCT165250.037.9957665
TTGTGGG5412050.037.9463963
GTGGGGA927450.037.929815
TGGGATA51450.037.8425646
Produced by FastQC (version 0.11.5)