##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4910685
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10804460070234	33.0	31.0	34.0	31.0	34.0
2	32.20124565106497	33.0	31.0	34.0	30.0	34.0
3	32.85015023362321	34.0	31.0	34.0	31.0	34.0
4	36.50622489530483	37.0	37.0	37.0	35.0	37.0
5	36.063490124086556	37.0	35.0	37.0	35.0	37.0
6	35.18500168510096	35.0	35.0	37.0	33.0	37.0
7	36.320278331841685	37.0	35.0	37.0	35.0	37.0
8	36.63353992365627	37.0	37.0	37.0	35.0	37.0
9	38.70455017986289	39.0	39.0	39.0	39.0	39.0
10	38.17850462817306	39.0	39.0	39.0	37.0	39.0
11	37.78174144747627	39.0	38.0	39.0	35.0	39.0
12	37.405275027821986	39.0	37.0	39.0	35.0	39.0
13	37.16421660114628	39.0	37.0	39.0	34.0	39.0
14	38.07141895682578	40.0	38.0	40.0	34.0	40.0
15	38.06003785622576	40.0	38.0	40.0	34.0	40.0
16	37.91666885577063	40.0	38.0	40.0	34.0	40.0
17	38.005236133044576	40.0	38.0	40.0	34.0	40.0
18	37.909052403076146	40.0	38.0	40.0	34.0	40.0
19	37.91513118841873	40.0	38.0	40.0	34.0	40.0
20	37.670824335097855	40.0	37.0	40.0	33.0	40.0
21	37.93554402288072	40.0	38.0	40.0	34.0	40.0
22	37.80927670986838	40.0	38.0	40.0	34.0	40.0
23	37.83079794366774	40.0	38.0	40.0	34.0	40.0
24	37.834033337507904	40.0	37.0	40.0	34.0	40.0
25	37.72926261814798	40.0	37.0	40.0	34.0	40.0
26	37.53313763762082	40.0	37.0	40.0	33.0	40.0
27	37.38016183078328	39.0	37.0	40.0	33.0	40.0
28	37.19783757255861	39.0	36.0	40.0	33.0	40.0
29	37.4834812251244	40.0	37.0	40.0	33.0	40.0
30	37.50630431396027	40.0	37.0	40.0	33.0	40.0
31	37.45928704447547	40.0	37.0	40.0	33.0	40.0
32	36.709087632377155	40.0	36.0	40.0	31.0	40.0
33	36.90460903112295	40.0	35.0	40.0	31.0	40.0
34	36.87971046809152	40.0	35.0	40.0	31.0	40.0
35	36.90348189712841	40.0	35.0	40.0	31.0	40.0
36	36.66075567868841	40.0	35.0	40.0	31.0	40.0
37	36.523472794528665	39.0	35.0	40.0	31.0	40.0
38	36.296401011264216	39.0	35.0	40.0	30.0	40.0
39	36.203858117553864	39.0	35.0	40.0	30.0	40.0
40	36.00284216967694	39.0	35.0	40.0	30.0	40.0
41	35.81365613962207	39.0	35.0	40.0	29.0	40.0
42	35.6652984257797	38.0	35.0	40.0	29.0	40.0
43	35.49959934306517	38.0	35.0	40.0	28.0	40.0
44	35.336365089595446	38.0	35.0	40.0	28.0	40.0
45	35.17512057890091	37.0	35.0	40.0	28.0	40.0
46	34.830543600332746	37.0	34.0	40.0	26.0	40.0
47	34.70737137486929	37.0	34.0	40.0	26.0	40.0
48	34.55101498059843	36.0	34.0	40.0	26.0	40.0
49	34.21147803208717	36.0	33.0	40.0	24.0	40.0
50	33.690050980667664	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	6.0
13	17.0
14	26.0
15	63.0
16	179.0
17	424.0
18	859.0
19	1762.0
20	3038.0
21	5103.0
22	7829.0
23	11642.0
24	16580.0
25	23914.0
26	31934.0
27	42803.0
28	56929.0
29	73624.0
30	90078.0
31	106786.0
32	128363.0
33	171620.0
34	279996.0
35	336526.0
36	510601.0
37	924239.0
38	1539891.0
39	545848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.63982621569089	0.21779038973177875	0.12069599251428263	0.021687402063052304
8	98.97250587239866	0.759934713792475	0.1668606314597658	0.10069878234910201
9	96.13377767052866	2.112251142152266	0.6439834768469165	1.1099877104721643
10	55.33439835786657	27.983142881288455	7.0131560057303615	9.669302755114613
11	31.202204987695197	28.69127219522327	20.194229521950604	19.912293295130922
12	32.11482715751469	18.830448297946216	26.026165392404522	23.02855915213458
13	24.117022370606136	18.86502595870026	30.382685918563297	26.635265752130305
14	27.252633797525196	20.44602738721787	27.954837257938557	24.34650155731838
15	25.46665078293558	18.92461031403969	27.816770980016024	27.791967923008702
16	28.619143765075545	22.32085747711368	25.636443795519366	23.423554962291412
17	31.739360191093503	19.199154496775908	23.602389483340918	25.459095828789668
18	28.5534095548788	20.82175908249053	26.016370424899986	24.608460937730683
19	30.831951143272274	18.798640108253736	24.587364084643994	25.782044663829996
20	25.386030665782876	20.79768912076421	27.107114384245783	26.709165829207127
21	29.007541717703333	20.165251894593116	28.24127387523329	22.585932512470254
22	28.18618991036892	19.47453359358216	28.235409112985256	24.103867383063665
23	28.07553325045284	22.37034140858149	26.13386930743878	23.42025603352689
24	29.35230013735355	20.066772761844835	27.310487233451138	23.270439867350483
25	28.262146726984117	18.74579615674799	27.130410523175485	25.86164659309241
26	30.774586437533664	18.36621571125006	24.958737935746235	25.90045991547004
27	29.759656748498426	17.60017187011588	26.170748072824875	26.469423308560824
28	32.25209924888279	21.365267778324203	21.437864574901468	24.944768397891536
29	33.1271706493086	20.52990570561948	22.252435250886588	24.090488394185332
30	31.255924580786594	21.67274015743221	22.486027916675575	24.585307345105623
31	31.496766744354403	23.14752422523538	21.115302651259448	24.24040637915077
32	31.927521313218016	24.111748157334464	21.96184442699949	21.99888610244803
33	31.443861701575237	23.575570414310835	22.266302969952257	22.71426491416167
34	28.315947775106732	25.424681078098065	24.15322913198464	22.106142014810562
35	29.009761367304154	27.894071804646398	22.505434577864392	20.590732250185056
36	29.406488096874465	27.235731878546478	20.79528619734314	22.562493827235915
37	28.696424225948107	27.944431377699853	21.087241393003218	22.271903003348818
38	29.850764200921056	27.079602947450304	20.728411616709277	22.341221234919363
39	28.8583568280189	25.958130077575735	20.930399730383847	24.253113364021516
40	27.560309814211255	26.713910584775853	21.872691895326213	23.853087705686683
41	26.203757724227884	25.96377083848791	23.09252171540223	24.739949721881977
42	25.56268626474718	25.093423829873025	23.063523724286938	26.280366181092862
43	25.089065985702607	24.72272198277837	25.160115136686635	25.02809689483239
44	25.623573900586173	24.84433434439391	24.317829386327976	25.21426236869194
45	26.173945182800363	25.006429856527145	23.486356791364138	25.333268169308354
46	26.49041834285848	25.34514024010907	23.31418121911709	24.85026019791536
47	26.26010424207621	25.885085278326752	23.473507260188754	24.38130321940829
48	27.41982024910985	25.813852853522473	23.847915311204037	22.918411586163643
49	26.796729987771563	26.34665021275036	23.037865389451778	23.8187544100263
50	24.658087415503132	26.858635811500843	22.788226082511912	25.695050690484116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.5
2	15.0
3	76.0
4	137.0
5	442.5
6	748.0
7	8726.0
8	16704.0
9	13593.0
10	10482.0
11	10028.5
12	9575.0
13	9468.0
14	9361.0
15	10075.5
16	10790.0
17	13115.5
18	15441.0
19	16865.5
20	18290.0
21	21509.5
22	24729.0
23	30918.0
24	37107.0
25	45540.0
26	53973.0
27	68909.0
28	83845.0
29	98735.5
30	113626.0
31	107198.0
32	100770.0
33	94953.5
34	89137.0
35	93767.5
36	98398.0
37	100659.0
38	102920.0
39	107205.5
40	111491.0
41	117582.0
42	123673.0
43	141402.5
44	159132.0
45	175710.0
46	192288.0
47	224036.5
48	255785.0
49	279686.5
50	303588.0
51	328781.0
52	353974.0
53	390075.5
54	426177.0
55	421551.5
56	416926.0
57	407222.0
58	397518.0
59	395240.5
60	392963.0
61	345705.0
62	298447.0
63	255960.5
64	213474.0
65	182065.5
66	150657.0
67	133949.0
68	117241.0
69	99894.5
70	82548.0
71	76365.0
72	70182.0
73	50455.5
74	30729.0
75	20791.0
76	10853.0
77	7575.5
78	4298.0
79	2908.0
80	1518.0
81	1084.0
82	650.0
83	489.0
84	328.0
85	233.5
86	139.0
87	94.0
88	49.0
89	29.0
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4910685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.517676367255515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.30572102622195	5.647706729988877
2	10.8362556675889	1.845994376187212
3	4.903254804900813	1.252930127230071
4	2.757300550545019	0.9394317494719171
5	1.699954646626974	0.7239831759490387
6	1.1345769169347644	0.5798375395325319
7	0.8188096199882138	0.48820487446185534
8	0.6218468912882191	0.4237352455981579
9	0.4964840219532705	0.3806001198460206
>10	5.0323111510120615	10.502814561642753
>50	2.719835248571911	16.79156135888478
>100	2.4854141067566093	37.80499508547268
>500	0.12414449199025843	7.199006172506785
>1k	0.05601641711755563	8.737126216994945
>5k	0.005046524064644652	2.8414148981583667
>10k+	0.003027914438786791	3.840657768074081
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	26185	0.5332249981418071	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	20958	0.4267836360915025	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	19364	0.39432380614924395	No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	16252	0.3309517918579587	No Hit
TATTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	14863	0.30266653226586515	No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	13958	0.28423733145172214	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12606	0.2567055308984388	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	11743	0.23913160791213445	No Hit
TATTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	11147	0.22699480825994744	No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	10821	0.2203562232152948	No Hit
TATTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	10617	0.2162020166229355	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	10185	0.20740487325088047	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGA	9694	0.19740626816829016	No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	8794	0.17907888614317555	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	8340	0.1698337400993955	No Hit
TATTGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	8169	0.16635153751462373	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAA	7268	0.14800379173170342	No Hit
TATTGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC	7218	0.14698560384141926	No Hit
TATTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	7159	0.14578414213088398	No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	6748	0.1374146376727483	No Hit
TATTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	6481	0.13197751433863095	No Hit
TATTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	6208	0.1264182084576795	No Hit
TATTGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	5928	0.12071635627208832	No Hit
TATTGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	5880	0.11973889589741553	No Hit
TATTGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	5740	0.11688796980461992	No Hit
TATTGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	5667	0.11540141548480506	No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	5652	0.11509595911771983	No Hit
TATTGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	5633	0.11470904771941186	No Hit
TATTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	5520	0.11240794308736968	No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	5479	0.11157302901733668	No Hit
TATTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5323	0.10839628279965016	No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5305	0.10802973515914786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.647288514738779E-4	0.0	0.0	0.0	0.0
8	0.0023011046320421695	0.0	0.0	0.0	0.0
9	0.00531494078728324	0.0	0.0	0.0	0.0
10	0.04029987669744649	0.0	0.0	0.0	0.0
11	0.07062151207010835	0.0	0.0	0.0	0.0
12	0.08532414520581141	0.0	0.0	0.0	0.0
13	0.10010823337273721	0.0	0.0	0.0	0.0
14	0.11996289723327805	0.0	0.0	0.0	0.0
15	0.13910482957061998	0.0	0.0	0.0	0.0
16	0.16356170269524517	0.0	0.0	0.0	0.0
17	0.17814215328411412	0.0	0.0	0.0	0.0
18	0.19103241197511142	0.0	0.0	0.0	0.0
19	0.20626450281376224	0.0	0.0	0.0	0.0
20	0.22102822722288235	0.0	0.0	0.0	0.0
21	0.2345701261636615	0.0	0.0	0.0	0.0
22	0.2501687646428146	0.0	0.0	0.0	0.0
23	0.2665615896763893	0.0	0.0	0.0	0.0
24	0.28431878648294484	0.0	0.0	0.0	0.0
25	0.2984105068844774	0.0	0.0	0.0	0.0
26	0.31233931722356456	0.0	0.0	0.0	0.0
27	0.3282637758276086	0.0	0.0	0.0	0.0
28	0.35724140318509534	0.0	0.0	0.0	0.0
29	0.3734916819140303	0.0	0.0	0.0	0.0
30	0.3936518021416564	0.0	0.0	0.0	0.0
31	0.41454501765028706	0.0	0.0	0.0	0.0
32	0.4358658720728371	0.0	0.0	0.0	0.0
33	0.4550281681679847	0.0	0.0	0.0	0.0
34	0.4776115755744871	0.0	0.0	0.0	0.0
35	0.52019219314617	0.0	0.0	0.0	0.0
36	0.5440381535366248	0.0	0.0	0.0	0.0
37	0.5654608267482032	0.0	0.0	0.0	0.0
38	0.5856413107336349	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.5294157E-6	44.0	36
CGAATTA	25	4.444976E-5	44.0	38
GTGGGAT	32130	0.0	40.042328	5
GTGGGGG	82865	0.0	39.932663	5
GTGGGGC	66030	0.0	39.5287	5
TGGGGCT	32735	0.0	39.477013	6
TGGGATT	12505	0.0	39.408237	6
TGTGGGG	292800	0.0	39.34604	4
TACGTTA	45	2.3535904E-8	39.11111	31
CGATTAA	85	0.0	38.823532	30
TGGGGGT	16770	0.0	38.673824	6
TGGGGGC	23510	0.0	38.572525	6
TGGGGTC	12445	0.0	38.537567	6
GTGGGAC	23155	0.0	38.356293	5
TGGGAGA	15055	0.0	38.21322	6
GTGGGGT	54650	0.0	38.126625	5
GTGGGCT	16525	0.0	37.995766	5
TTGTGGG	541205	0.0	37.946396	3
GTGGGGA	92745	0.0	37.92981	5
TGGGATA	5145	0.0	37.842564	6
>>END_MODULE