FastQCFastQC Report
Fri 17 Jun 2016
SRR1527284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5267709
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA254720.4835498695922649No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC190750.362111878237769No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG183340.3480450419717566No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA168790.3204239262267525No Hit
TATTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG140010.26578916944728725No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT129280.2454197830593907No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121310.23028986604992796No Hit
TATTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA106360.20190940691674503No Hit
TATTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG105190.199688327506322No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA104640.19864423034757617No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC103010.19554990604074748No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT99700.1892663395035679No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG89960.1707763280014139No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC82260.15615896777897184No Hit
TATTGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT77220.14659124108791888No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA72790.13818151306383858No Hit
TATTGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG66960.12711408318113246No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA65150.12367805434962333No Hit
TATTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA64490.1224251377591283No Hit
TATTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA62720.11906504326643708No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT62310.1182867162935538No Hit
TATTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC61650.11703379970305877No Hit
TATTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC59960.1138255738880033No Hit
TATTGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG55830.1059853534050571No Hit
TATTGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC55510.10537787869451405No Hit
TATTGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG54420.10330866796172682No Hit
TATTGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG54160.10281509475941059No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC53430.10142929307598426No Hit
TATTGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG53300.10118250647482616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC206.3135254E-446.024
GTGGGAT339750.041.504935
GTGGGGG900950.041.4456945
GTGGGGC713200.041.2174725
TGTGGGG3168800.040.9155854
TGGGGCT357550.040.8860326
TCGTATA402.9132207E-740.2531
TGGGATT128100.040.2006266
TGGGGGT191600.040.081946
GTGGGAC246050.039.961395
GTGGGGT594250.039.818935
TGGGGGC246000.039.5861786
TGGGGCC149950.039.481166
TGGGATA53750.039.4102336
TTGTGGG5827100.039.398123
GTGGGCT180050.039.3957255
TGGGGTC138650.039.3486
ATTGTGG6077200.039.3269232
TGGGGGG339250.039.2813576
TATTGTG6167700.039.1522451
Produced by FastQC (version 0.11.5)