##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5267709
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06557309828618	33.0	31.0	34.0	30.0	34.0
2	32.029162582823005	33.0	31.0	34.0	30.0	34.0
3	32.747982092404875	34.0	31.0	34.0	31.0	34.0
4	36.4588040075866	37.0	37.0	37.0	35.0	37.0
5	36.14843340814764	37.0	37.0	37.0	35.0	37.0
6	35.18250799351293	35.0	35.0	37.0	33.0	37.0
7	36.329500167909806	37.0	35.0	37.0	35.0	37.0
8	36.607267789469766	37.0	37.0	37.0	35.0	37.0
9	38.66798697498286	39.0	39.0	39.0	38.0	39.0
10	38.08597228890206	39.0	38.0	39.0	37.0	39.0
11	37.73846467221329	39.0	38.0	39.0	35.0	39.0
12	37.44722421075272	39.0	37.0	39.0	35.0	39.0
13	37.275697841319634	39.0	37.0	39.0	34.0	39.0
14	38.16639586583086	40.0	38.0	40.0	35.0	40.0
15	38.07595123420827	40.0	38.0	40.0	34.0	40.0
16	33.008845591128896	40.0	34.0	40.0	3.0	40.0
17	34.75957574725559	40.0	35.0	40.0	17.0	40.0
18	36.758451919041086	40.0	34.0	40.0	31.0	40.0
19	37.64618015156115	40.0	37.0	40.0	33.0	40.0
20	37.906614241599144	40.0	38.0	40.0	34.0	40.0
21	38.1144005866687	40.0	38.0	40.0	34.0	40.0
22	38.16983037597559	40.0	38.0	40.0	34.0	40.0
23	38.09335101844084	40.0	38.0	40.0	34.0	40.0
24	38.04476974715194	40.0	38.0	40.0	34.0	40.0
25	37.930073775905235	40.0	37.0	40.0	34.0	40.0
26	37.763577866582985	40.0	37.0	40.0	34.0	40.0
27	37.62651012043376	40.0	37.0	40.0	33.0	40.0
28	37.52466470718105	40.0	37.0	40.0	33.0	40.0
29	37.32813961439404	40.0	36.0	40.0	33.0	40.0
30	37.302159439710884	40.0	36.0	40.0	33.0	40.0
31	37.223557527570335	40.0	36.0	40.0	33.0	40.0
32	37.11430035334146	39.0	36.0	40.0	32.0	40.0
33	36.79214189698026	39.0	35.0	40.0	31.0	40.0
34	36.710889686579115	39.0	35.0	40.0	31.0	40.0
35	36.66194298128465	39.0	35.0	40.0	31.0	40.0
36	36.563822337186814	39.0	35.0	40.0	31.0	40.0
37	36.183412940995794	39.0	35.0	40.0	30.0	40.0
38	36.14906461233907	38.0	35.0	40.0	30.0	40.0
39	35.71431926099183	38.0	35.0	40.0	29.0	40.0
40	35.58941904345893	38.0	35.0	40.0	28.0	40.0
41	35.481514639476096	38.0	34.0	40.0	29.0	40.0
42	35.37557522634603	38.0	34.0	40.0	29.0	40.0
43	35.21399112973021	37.0	34.0	40.0	28.0	40.0
44	35.48054875468633	37.0	35.0	40.0	29.0	40.0
45	35.49356807674835	37.0	35.0	40.0	30.0	40.0
46	35.376211935777015	37.0	35.0	40.0	29.0	40.0
47	35.04360909078311	37.0	34.0	40.0	28.0	40.0
48	34.95831565487008	36.0	34.0	40.0	28.0	40.0
49	34.83129554043323	36.0	34.0	40.0	28.0	40.0
50	34.326877965354576	35.0	34.0	40.0	26.0	40.0
51	34.34635417408213	35.0	34.0	39.0	26.0	40.0
52	33.890343221313096	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	8.0
14	19.0
15	62.0
16	133.0
17	394.0
18	876.0
19	1658.0
20	3086.0
21	5245.0
22	8612.0
23	13394.0
24	19225.0
25	26753.0
26	36476.0
27	48636.0
28	62496.0
29	75220.0
30	91704.0
31	117001.0
32	156148.0
33	220798.0
34	328566.0
35	448574.0
36	699101.0
37	1115360.0
38	1323268.0
39	464891.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.6403370041891	0.22039941841889898	0.1187992730805745	0.020464304311418872
8	98.95130881375566	0.7581474223424263	0.18770968555780132	0.10283407834411507
9	96.02206575951709	2.1476129376167137	0.6658492335092921	1.1644720693569064
10	55.48618953704542	27.65969038912362	7.200075023126752	9.654045050704205
11	31.33436186395262	28.538231705661797	20.070262043708183	20.057144386677397
12	32.20838508733113	18.807720775767987	25.992874701317024	22.991019435583855
13	24.164261921074228	18.829248160822857	30.353176304917373	26.653313613185542
14	27.409315890456366	20.33128633339465	27.888689371413644	24.370708404735343
15	25.665293963656687	18.95085700443969	27.716394356635877	27.66745467526775
16	27.178703303466463	29.77637906725675	22.633311748997524	20.411605880279264
17	31.85003195886485	19.199769767084703	23.4748920261161	25.47530624793435
18	28.8006797642011	20.67242135053398	25.952325764388274	24.574573120876646
19	30.83270165455229	18.775486648939797	24.54685708720812	25.84495460929979
20	25.46125839525304	20.642617122547964	27.112108888323178	26.78401559387582
21	29.074840694503056	19.982140243510035	28.36768318067684	22.57533588131007
22	28.086593241957747	19.35000585643588	28.38305988428746	24.180341017318916
23	28.22980540496827	22.25240612190233	26.02136906195843	23.496419411170965
24	29.425562421918144	20.106691542756064	27.203514848675205	23.26423118665059
25	28.503415811313797	18.532534731891985	27.05079190972774	25.913257547066475
26	30.893980665978322	18.13344283064991	25.033045675074305	25.939530828297464
27	29.94835136109455	17.492519043857584	26.044604969636705	26.514524625411163
28	32.219680320230296	21.28747051137411	21.39432910967557	25.098520058720027
29	33.38253878488732	20.419465084346914	22.235434797176534	23.962561333589232
30	31.419712060783922	21.520588931545003	22.535052714567186	24.524646293103892
31	31.560475341367567	23.076844222032765	21.106537965555805	24.256142471043866
32	31.94588007803772	23.69470295340916	22.30166852421043	22.057748444342693
33	31.727796656952766	23.320460564545233	22.36363094468582	22.58811183381618
34	28.340460720210626	25.268499076163852	24.179923378455413	22.21111682517011
35	29.102101122138674	27.779761562379395	22.63353955201398	20.484597763467953
36	29.57980404764196	27.063415993556212	20.879380390982114	22.47739956781971
37	29.055059799241	27.798175639542734	20.981094437828666	22.165670123387603
38	29.909226193018633	26.96211958557316	20.774477101905212	22.354177119502992
39	29.270048896019123	25.804500590294566	20.828181663034158	24.097268850652153
40	27.64131427912969	26.59898259376135	21.92157919125753	23.838123935851428
41	26.189829392625903	25.956900808302052	23.106401663417625	24.746868135654417
42	25.658934462780692	25.04257543459595	23.05469037868265	26.243799723940715
43	25.17690327996478	24.585906320945217	25.159495332790783	25.07769506629922
44	25.67457693657717	24.73094090808737	24.392615461484297	25.20186669385116
45	26.24040166227861	24.882866536477245	23.463122203599326	25.41360959764482
46	26.21424228255585	25.363606835533247	23.510771001207544	24.911379880703358
47	26.57593652193012	25.836222160335737	23.39867293352765	24.189168384206493
48	27.58354723087399	25.705064573612553	23.848280153668323	22.863108041845138
49	26.86815084128603	26.181533566110048	23.095429151458443	23.854886441145474
50	24.63344121704521	26.82771580586551	22.752281874340436	25.78656110274884
51	25.082516896814155	27.67123240862394	22.041479512250962	25.204771182310942
52	26.533299390683883	27.15873257235736	22.070296593832346	24.237671443126413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.5
2	15.0
3	75.0
4	135.0
5	459.0
6	783.0
7	8474.5
8	16166.0
9	13276.5
10	10387.0
11	10321.5
12	10256.0
13	9867.5
14	9885.0
15	10291.0
16	12139.0
17	13987.0
18	14953.5
19	15920.0
20	19053.0
21	22186.0
22	28121.5
23	34057.0
24	41635.0
25	49213.0
26	67649.0
27	86085.0
28	97581.0
29	109077.0
30	101719.5
31	94362.0
32	91710.0
33	89058.0
34	92633.5
35	96209.0
36	100984.0
37	105759.0
38	102927.5
39	106065.0
40	112034.0
41	121838.5
42	131643.0
43	153247.5
44	174852.0
45	188519.5
46	202187.0
47	227508.0
48	252829.0
49	288453.0
50	324077.0
51	343618.5
52	363160.0
53	392261.0
54	421362.0
55	445517.0
56	469672.0
57	440182.0
58	410692.0
59	406993.0
60	403294.0
61	364081.5
62	324869.0
63	289641.0
64	211032.0
65	167651.0
66	151371.5
67	135092.0
68	117204.0
69	99316.0
70	92644.5
71	85973.0
72	61779.0
73	37585.0
74	25700.0
75	13815.0
76	10115.0
77	6415.0
78	4320.5
79	2226.0
80	1456.5
81	687.0
82	439.5
83	192.0
84	135.0
85	78.0
86	68.5
87	59.0
88	34.5
89	7.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5267709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.61799196762016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.35348918359736	7.470127830454887
2	10.201778516697345	2.1664480469146463
3	4.27822912999819	1.3627860761387809
4	2.432506422481182	1.0331333462035857
5	1.4768688843865851	0.7840690975822453
6	1.017399983310567	0.6481646910389092
7	0.7046018578219123	0.5237019806706316
8	0.5193952896250393	0.44119480106067316
9	0.42226290500434166	0.403522572020405
>10	4.316073037282543	11.208285475403377
>50	2.240893944264137	17.212362837809376
>100	1.8976212125085763	35.697136854844636
>500	0.08971870097896195	6.4961920480652395
>1k	0.04260614110416458	8.128173226401241
>5k	0.0043015815537858475	2.9286705349191173
>10k+	0.002253209385316396	3.4960305804721656
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	25472	0.4835498695922649	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	19075	0.362111878237769	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	18334	0.3480450419717566	No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	16879	0.3204239262267525	No Hit
TATTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	14001	0.26578916944728725	No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	12928	0.2454197830593907	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12131	0.23028986604992796	No Hit
TATTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	10636	0.20190940691674503	No Hit
TATTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	10519	0.199688327506322	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	10464	0.19864423034757617	No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	10301	0.19554990604074748	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9970	0.1892663395035679	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG	8996	0.1707763280014139	No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	8226	0.15615896777897184	No Hit
TATTGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	7722	0.14659124108791888	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	7279	0.13818151306383858	No Hit
TATTGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	6696	0.12711408318113246	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	6515	0.12367805434962333	No Hit
TATTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	6449	0.1224251377591283	No Hit
TATTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	6272	0.11906504326643708	No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	6231	0.1182867162935538	No Hit
TATTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6165	0.11703379970305877	No Hit
TATTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5996	0.1138255738880033	No Hit
TATTGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	5583	0.1059853534050571	No Hit
TATTGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5551	0.10537787869451405	No Hit
TATTGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5442	0.10330866796172682	No Hit
TATTGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5416	0.10281509475941059	No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5343	0.10142929307598426	No Hit
TATTGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	5330	0.10118250647482616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.2272093997599334E-4	0.0	0.0	0.0	0.0
8	0.002107177902196192	0.0	0.0	0.0	0.0
9	0.005030649946684602	0.0	0.0	0.0	0.0
10	0.033468059833980955	0.0	0.0	0.0	0.0
11	0.06067153671548675	0.0	0.0	0.0	0.0
12	0.07247932640166721	0.0	0.0	0.0	0.0
13	0.08500849230661754	0.0	0.0	0.0	0.0
14	0.10328968437702235	0.0	0.0	0.0	0.0
15	0.12075458230513493	0.0	0.0	0.0	0.0
16	0.14448406318572268	0.0	0.0	0.0	1.89835847044702E-5
17	0.15815224417294121	0.0	0.0	0.0	1.89835847044702E-5
18	0.1710800653566854	0.0	0.0	0.0	1.89835847044702E-5
19	0.18533673746974255	0.0	0.0	0.0	1.89835847044702E-5
20	0.1987391482710985	0.0	0.0	0.0	1.89835847044702E-5
21	0.21187578888659186	0.0	0.0	0.0	1.89835847044702E-5
22	0.22795488513127812	0.0	0.0	0.0	1.89835847044702E-5
23	0.24200273781258608	0.0	0.0	0.0	1.89835847044702E-5
24	0.259562553664221	0.0	0.0	0.0	1.89835847044702E-5
25	0.272319522585625	0.0	0.0	0.0	1.89835847044702E-5
26	0.286006687157548	0.0	0.0	0.0	1.89835847044702E-5
27	0.29988368757651573	0.0	0.0	0.0	1.89835847044702E-5
28	0.3259481493757533	0.0	0.0	0.0	1.89835847044702E-5
29	0.3410021320463982	0.0	0.0	0.0	1.89835847044702E-5
30	0.3600047003355728	0.0	0.0	0.0	1.89835847044702E-5
31	0.3805829061552185	0.0	0.0	0.0	1.89835847044702E-5
32	0.4006105880184346	0.0	0.0	0.0	1.89835847044702E-5
33	0.41921450102881536	0.0	0.0	0.0	1.89835847044702E-5
34	0.4424693922917914	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
35	0.48603671918855046	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
36	0.5100319702550008	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
37	0.5309708641840314	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
38	0.552270446222447	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
39	0.5734371431679313	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
40	0.5971666240485191	0.0	1.89835847044702E-5	0.0	1.89835847044702E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTC	20	6.3135254E-4	46.0	24
GTGGGAT	33975	0.0	41.50493	5
GTGGGGG	90095	0.0	41.445694	5
GTGGGGC	71320	0.0	41.217472	5
TGTGGGG	316880	0.0	40.915585	4
TGGGGCT	35755	0.0	40.886032	6
TCGTATA	40	2.9132207E-7	40.25	31
TGGGATT	12810	0.0	40.200626	6
TGGGGGT	19160	0.0	40.08194	6
GTGGGAC	24605	0.0	39.96139	5
GTGGGGT	59425	0.0	39.81893	5
TGGGGGC	24600	0.0	39.586178	6
TGGGGCC	14995	0.0	39.48116	6
TGGGATA	5375	0.0	39.410233	6
TTGTGGG	582710	0.0	39.39812	3
GTGGGCT	18005	0.0	39.395725	5
TGGGGTC	13865	0.0	39.348	6
ATTGTGG	607720	0.0	39.326923	2
TGGGGGG	33925	0.0	39.281357	6
TATTGTG	616770	0.0	39.152245	1
>>END_MODULE