FastQCFastQC Report
Fri 17 Jun 2016
SRR1527283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5134949
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA236090.45977087601064787No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG179400.3493705585001915No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC178880.3483578902146838No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA164950.32123006479713817No Hit
TATTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG142770.27803586754220927No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT134440.2618137005839785No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110700.21558149847252622No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC104830.2041500314803516No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA104710.20391633879908058No Hit
TATTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA102850.20029410223937957No Hit
TATTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG99810.19437388764718014No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT98250.19133588279065672No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG90470.17618480728825153No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC80880.15750886717667498No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA73720.1435652038608368No Hit
TATTGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT73250.14264990752585857No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA67310.13108211980294254No Hit
TATTGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG66130.1287841417704441No Hit
TATTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA64090.12481136618883654No Hit
TATTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC62210.12115018084892372No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT62180.12109175767860596No Hit
TATTGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG55620.10831655776912294No Hit
TATTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA55620.10831655776912294No Hit
TATTGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC55160.10742073582425064No Hit
TATTGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG54100.1053564504730232No Hit
TATTGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG52840.10290267731967737No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG52500.10224054805607612No Hit
TATTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC52240.10173421391332221No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51850.10097471269919137No Hit
TATTGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG51490.10027363465537828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGGG874200.041.585225
GTGGGAT335200.041.4644975
GTGGGGC685450.041.4063725
TGGGGCT337250.041.05566
TGTGGGG3068700.041.02634
TGGGATT124750.040.6901786
CGATTAG402.9132025E-740.2530
GTGGGAC243550.039.9749535
GTGGGGT565450.039.9393425
TGGGGGC243350.039.894396
TGGGGGT179550.039.8641056
TGGGGTC127750.039.8246576
TGGGACT79650.039.589456
TGGGATA53750.039.5813946
TGGGGCC142850.039.5596776
TTGTGGG5677600.039.461273
TCGTCTA355.3767544E-639.42857437
ATTGTGG5914550.039.4129072
TATTGTG5985350.039.317521
GTGGGCT168050.039.2799765
Produced by FastQC (version 0.11.5)