##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527283_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5134949
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07123303464163	33.0	31.0	34.0	30.0	34.0
2	32.07746269729261	33.0	31.0	34.0	30.0	34.0
3	32.743778175791036	34.0	31.0	34.0	31.0	34.0
4	36.459079340417986	37.0	37.0	37.0	35.0	37.0
5	36.16518586650033	37.0	37.0	37.0	35.0	37.0
6	35.207697486382045	35.0	35.0	37.0	33.0	37.0
7	36.35273865426901	37.0	35.0	37.0	35.0	37.0
8	36.63484856422138	37.0	37.0	37.0	35.0	37.0
9	38.61312176615581	39.0	39.0	39.0	38.0	39.0
10	38.08005084373769	39.0	39.0	39.0	37.0	39.0
11	37.66175886070144	39.0	38.0	39.0	35.0	39.0
12	37.40667473036246	39.0	37.0	39.0	35.0	39.0
13	37.170282703878854	39.0	37.0	39.0	34.0	39.0
14	38.08263431632914	40.0	38.0	40.0	34.0	40.0
15	38.18516074843197	40.0	38.0	40.0	34.0	40.0
16	38.23939186153553	40.0	38.0	40.0	35.0	40.0
17	38.24358820311555	40.0	38.0	40.0	34.0	40.0
18	38.18603203264531	40.0	38.0	40.0	34.0	40.0
19	38.176630186589975	40.0	38.0	40.0	34.0	40.0
20	38.143438425581245	40.0	38.0	40.0	34.0	40.0
21	33.89348268113276	40.0	34.0	40.0	10.0	40.0
22	34.66292576615659	40.0	34.0	40.0	17.0	40.0
23	36.666164746719005	39.0	34.0	40.0	30.0	40.0
24	37.42148753570873	39.0	37.0	40.0	33.0	40.0
25	37.63236947436089	39.0	37.0	40.0	33.0	40.0
26	37.62942553080858	40.0	37.0	40.0	34.0	40.0
27	37.5755381406904	40.0	37.0	40.0	33.0	40.0
28	37.48833707988142	40.0	37.0	40.0	33.0	40.0
29	37.49151744252961	40.0	37.0	40.0	33.0	40.0
30	37.371910217608786	40.0	36.0	40.0	33.0	40.0
31	37.189116776038084	40.0	36.0	40.0	33.0	40.0
32	37.11265330970181	39.0	36.0	40.0	32.0	40.0
33	36.80618989594639	39.0	35.0	40.0	31.0	40.0
34	36.70684148956494	39.0	35.0	40.0	31.0	40.0
35	36.6550211112126	39.0	35.0	40.0	31.0	40.0
36	36.49770932486379	39.0	35.0	40.0	31.0	40.0
37	36.33830131516399	39.0	35.0	40.0	31.0	40.0
38	36.23361517319841	39.0	35.0	40.0	30.0	40.0
39	36.007037070864776	38.0	35.0	40.0	30.0	40.0
40	31.090587267760593	37.0	30.0	40.0	8.0	40.0
41	31.92832548093467	36.0	29.0	40.0	13.0	40.0
42	33.650071694967174	36.0	30.0	40.0	23.0	40.0
43	34.45530267194474	36.0	33.0	40.0	26.0	40.0
44	35.186027748279486	37.0	34.0	40.0	28.0	40.0
45	35.385168577136795	37.0	35.0	40.0	29.0	40.0
46	35.35525318751949	37.0	35.0	40.0	29.0	40.0
47	35.25958193547784	37.0	35.0	40.0	29.0	40.0
48	35.077853743045935	36.0	34.0	40.0	29.0	40.0
49	34.88478931338948	36.0	34.0	40.0	28.0	40.0
50	34.75693448951489	36.0	34.0	40.0	28.0	40.0
51	34.52531992041206	35.0	34.0	40.0	27.0	40.0
52	34.013586892489094	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	16.0
15	53.0
16	147.0
17	432.0
18	902.0
19	1770.0
20	3200.0
21	5698.0
22	9259.0
23	13948.0
24	20629.0
25	28290.0
26	38158.0
27	50542.0
28	65476.0
29	78897.0
30	98173.0
31	127575.0
32	174683.0
33	229980.0
34	382569.0
35	525017.0
36	666012.0
37	839825.0
38	1290857.0
39	482833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.64673456347862	0.2180157972357661	0.11474310650407629	0.02050653278153298
8	98.97504337433536	0.7579237885322717	0.16627234272433863	0.10076049440802626
9	95.98103116506122	2.1467788677161157	0.7014870060053178	1.1707029612173363
10	55.37831047591709	27.6311020810528	7.30729750188366	9.683289941146446
11	31.350399001041684	28.5828934230895	20.048475651851653	20.01823192401716
12	32.28853879561414	18.90394627093667	25.87114302401056	22.936371909438634
13	24.26096150127294	18.93113251952454	30.291284295131266	26.51662168407125
14	27.445511143343392	20.327446290118946	27.811240189532555	24.415802377005107
15	25.666467183997348	18.776233220622053	27.741813988804953	27.815485606575646
16	28.33667870898036	22.252295008188007	25.668901482760585	23.742124800071043
17	31.84539904875394	19.290960825511608	23.413922903615987	25.44971722211847
18	28.822681588463684	20.724568053158855	25.904853193283905	24.54789716509356
19	30.880813032417652	18.868853419965806	24.532122909107763	25.718210638508776
20	25.455384269639293	20.695161724098917	27.078087825215015	26.771366181046787
21	33.70045155268338	19.340328404430114	26.62367240648349	20.33554763640301
22	28.205655012347737	19.319802397258474	28.367857207539938	24.10668538285385
23	28.133755564076683	22.312626668736147	26.02911927654978	23.524498490637395
24	29.538287527295793	20.14080373534382	27.17849778060113	23.14241095675926
25	28.526865602754768	18.554264122194787	27.071018621606562	25.847851653443882
26	30.90587657248397	18.15305273723264	25.06005415048913	25.88101653979426
27	30.04828285538961	17.517681285636915	26.027191311929293	26.40684454704419
28	32.282871748093314	21.259140061566338	21.36011477426553	25.097873416074822
29	33.23820742912928	20.425441421131936	22.369550311015747	23.96680083872303
30	31.44743988693948	21.55481972654451	22.587741377762466	24.409999008753545
31	31.570342762897933	23.209149691652247	21.082039957943106	24.138467587506714
32	31.918778550673043	23.737957280588375	22.349238522135273	21.99402564660331
33	31.700295368074737	23.405626813430864	22.379774365821355	22.514303452673047
34	28.40770180969665	25.32003725840315	24.14859427036179	22.12366666153841
35	29.058068541673933	27.811902318796157	22.663340960153644	20.466688179376273
36	29.695699022521936	27.09808802385379	20.856526520516564	22.34968643310771
37	29.019061338291774	27.854395438007273	20.959584992957087	22.16695823074387
38	29.921290357508905	27.058223947306974	20.73794695916162	22.2825387360225
39	29.02149563705501	25.947287889324706	20.833955702383804	24.197260771236483
40	28.026081661181056	30.556272321302508	20.197941595914585	21.21970442160185
41	26.23231506291494	26.05657816659912	23.05407512323881	24.65703164724713
42	25.644207956106285	25.155809726639934	22.978592387188264	26.22138993006552
43	25.16346316195156	24.749768692931518	25.12098951713055	24.965778627986374
44	25.644052160985435	24.857403647046933	24.336736353175077	25.161807838792555
45	26.21788453984645	25.01208872765825	23.427438130349493	25.342588602145806
46	26.189198763220432	25.462278203736783	23.475345130010055	24.87317790303273
47	26.353698936445134	25.952993885625737	23.34961846748624	24.343688710442887
48	27.508919757528265	25.877141136163186	23.779184564442605	22.834754541865944
49	26.855261853623087	26.299287490489192	23.07658751820125	23.76886313768647
50	24.53971792124907	27.003072474527013	22.76435462163305	25.69285498259087
51	25.08147597960564	27.78056802511573	22.003081238002558	25.134874757276073
52	26.538335629039356	27.241594804544313	22.067405148522408	24.152664417893927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	10.0
2	20.0
3	73.0
4	126.0
5	429.0
6	732.0
7	7907.0
8	15082.0
9	12464.5
10	9847.0
11	10014.0
12	10181.0
13	9657.0
14	9472.5
15	9812.0
16	11525.5
17	13239.0
18	14267.5
19	15296.0
20	18436.5
21	21577.0
22	26792.5
23	32008.0
24	38605.5
25	45203.0
26	63453.0
27	81703.0
28	93569.0
29	105435.0
30	99929.5
31	94424.0
32	91664.0
33	88904.0
34	91860.5
35	94817.0
36	99447.5
37	104078.0
38	100991.0
39	104276.0
40	110648.0
41	118821.5
42	126995.0
43	148183.0
44	169371.0
45	183238.0
46	197105.0
47	221439.0
48	245773.0
49	281215.5
50	316658.0
51	334122.0
52	351586.0
53	379257.5
54	406929.0
55	432036.5
56	457144.0
57	427434.5
58	397725.0
59	398284.5
60	398844.0
61	358001.0
62	317158.0
63	283823.5
64	207277.0
65	164065.0
66	147971.0
67	131877.0
68	113793.0
69	95709.0
70	90540.5
71	85372.0
72	61621.5
73	37871.0
74	26110.5
75	14350.0
76	10382.0
77	6414.0
78	4367.0
79	2320.0
80	1557.5
81	795.0
82	471.0
83	147.0
84	95.0
85	43.0
86	35.0
87	27.0
88	17.0
89	6.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5134949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.82270989664172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.31522686641188	7.610013016917236
2	10.031841951951572	2.1714343034986374
3	4.498612860190568	1.4606154576943253
4	2.432551867433174	1.0530721267905325
5	1.4903869698416288	0.806501290416543
6	0.995325818639621	0.6463273552664429
7	0.6629992095816848	0.5022813674903737
8	0.5011485435545685	0.43390282416124903
9	0.4201284376858734	0.4092235380363174
>10	4.425803952483076	11.82586078804825
>50	2.2638523808078066	17.62784595159449
>100	1.8287532953030787	34.89807132153469
>500	0.08724219041670811	6.425960266189043
>1k	0.03965554109850369	7.735818899188798
>5k	0.004382980858255671	3.0960171752123267
>10k+	0.00208713374202651	3.297054317960789
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	23609	0.45977087601064787	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	17940	0.3493705585001915	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17888	0.3483578902146838	No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	16495	0.32123006479713817	No Hit
TATTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	14277	0.27803586754220927	No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	13444	0.2618137005839785	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11070	0.21558149847252622	No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	10483	0.2041500314803516	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	10471	0.20391633879908058	No Hit
TATTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	10285	0.20029410223937957	No Hit
TATTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	9981	0.19437388764718014	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9825	0.19133588279065672	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG	9047	0.17618480728825153	No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	8088	0.15750886717667498	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	7372	0.1435652038608368	No Hit
TATTGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	7325	0.14264990752585857	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	6731	0.13108211980294254	No Hit
TATTGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	6613	0.1287841417704441	No Hit
TATTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	6409	0.12481136618883654	No Hit
TATTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6221	0.12115018084892372	No Hit
TATTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	6218	0.12109175767860596	No Hit
TATTGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	5562	0.10831655776912294	No Hit
TATTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	5562	0.10831655776912294	No Hit
TATTGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5516	0.10742073582425064	No Hit
TATTGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	5410	0.1053564504730232	No Hit
TATTGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5284	0.10290267731967737	No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	5250	0.10224054805607612	No Hit
TATTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5224	0.10173421391332221	No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5185	0.10097471269919137	No Hit
TATTGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5149	0.10027363465537828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.7264146148286967E-4	0.0	0.0	0.0	0.0
8	0.002765363395040535	0.0	0.0	0.0	0.0
9	0.005764419471352101	0.0	0.0	0.0	0.0
10	0.03797506070654256	0.0	0.0	0.0	0.0
11	0.06496656539334665	0.0	0.0	0.0	0.0
12	0.07877390797844341	0.0	0.0	0.0	0.0
13	0.09174385178898564	0.0	0.0	0.0	0.0
14	0.11006925287865565	0.0	0.0	0.0	0.0
15	0.1283946539683257	0.0	0.0	0.0	0.0
16	0.15160812697458145	0.0	0.0	0.0	0.0
17	0.16393541591162833	0.0	0.0	0.0	0.0
18	0.17526951095327334	0.0	0.0	0.0	0.0
19	0.18792786452212087	0.0	0.0	0.0	1.947439010591926E-5
20	0.2023973363708189	0.0	0.0	0.0	1.947439010591926E-5
21	0.2138482777530994	0.0	0.0	0.0	1.947439010591926E-5
22	0.2287267117940217	0.0	0.0	0.0	1.947439010591926E-5
23	0.24455939095013407	0.0	0.0	0.0	1.947439010591926E-5
24	0.2620863420454614	0.0	0.0	0.0	1.947439010591926E-5
25	0.2759131590206641	0.0	0.0	0.0	1.947439010591926E-5
26	0.28975945038597267	0.0	0.0	0.0	1.947439010591926E-5
27	0.30528053930039034	0.0	0.0	0.0	1.947439010591926E-5
28	0.33301207081121936	0.0	0.0	0.0	1.947439010591926E-5
29	0.34730627314896406	0.0	0.0	0.0	1.947439010591926E-5
30	0.3653200839969394	0.0	0.0	0.0	1.947439010591926E-5
31	0.38465815337211723	0.0	0.0	0.0	1.947439010591926E-5
32	0.40510626298333247	0.0	0.0	0.0	1.947439010591926E-5
33	0.42210740554579995	0.0	0.0	0.0	1.947439010591926E-5
34	0.442594463937227	0.0	0.0	0.0	1.947439010591926E-5
35	0.4796347539186855	0.0	0.0	0.0	1.947439010591926E-5
36	0.4994012598761935	0.0	0.0	0.0	1.947439010591926E-5
37	0.5178240329163931	0.0	0.0	0.0	1.947439010591926E-5
38	0.5380968730166551	0.0	0.0	0.0	1.947439010591926E-5
39	0.5586813033586118	0.0	0.0	0.0	1.947439010591926E-5
40	0.583452727573341	0.0	0.0	0.0	1.947439010591926E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGGGG	87420	0.0	41.58522	5
GTGGGAT	33520	0.0	41.464497	5
GTGGGGC	68545	0.0	41.406372	5
TGGGGCT	33725	0.0	41.0556	6
TGTGGGG	306870	0.0	41.0263	4
TGGGATT	12475	0.0	40.690178	6
CGATTAG	40	2.9132025E-7	40.25	30
GTGGGAC	24355	0.0	39.974953	5
GTGGGGT	56545	0.0	39.939342	5
TGGGGGC	24335	0.0	39.89439	6
TGGGGGT	17955	0.0	39.864105	6
TGGGGTC	12775	0.0	39.824657	6
TGGGACT	7965	0.0	39.58945	6
TGGGATA	5375	0.0	39.581394	6
TGGGGCC	14285	0.0	39.559677	6
TTGTGGG	567760	0.0	39.46127	3
TCGTCTA	35	5.3767544E-6	39.428574	37
ATTGTGG	591455	0.0	39.412907	2
TATTGTG	598535	0.0	39.31752	1
GTGGGCT	16805	0.0	39.279976	5
>>END_MODULE