FastQCFastQC Report
Fri 17 Jun 2016
SRR1527282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012255
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC70800.699428503687312No Hit
GCAGGAGGGATCAAGCAAGAGTACAATATGAAGATTCTTTTCTTGGCCCT40990.4049374910472163No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG38040.3757946367269117No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT37050.36601449239569084No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC35570.35139367056719895No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG25860.2554692246518911No Hit
GCAGGAGGGGGTTAGGCTGGCCAGTACTTCTGGGTCTTGAGAACTGAGCC25420.2511224938380151No Hit
GCAGGAGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCT23970.23679804001956029No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC18900.18671184632330787No Hit
GCAGGAGGGATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGATT16490.16290361618366914No Hit
GCAGGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA16250.16053267210337316No Hit
GCAGGAGGGACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCA14180.14008327941082038No Hit
GCAGGAGGGCCCTTTGACCTTTACCTCCACCGCCAGGCAGGCACTACCAT14110.1393917540540674No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC13870.13702080997377145No Hit
GCAGGAGAGGATGTTTCTGTGGGCCCCTTCACCCCTCCTCTGTCTGCACC13480.13316802584329046No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC13420.13257528982321648No Hit
GCAGGAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGAT13090.12931524171280953No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTG12990.12832734834601955No Hit
GCAGGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA12000.11854720401479864No Hit
GCAGGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTT11430.11291621182409571No Hit
GCAGGAGGGGACTTTTGTTTGCATTTATTTGAAACCATTTGGAATATTGT11210.11074284641715774No Hit
GCAGGAGGGATATTAAGGAAACAAACAAACTCATCTTACTGTGTGCTATT10880.10748279830675075No Hit
GCAGGAGGGGACCCGCCGGGCTTCCCAATCATCACTGGAAAGTTCGTCTG10460.1033336461662328No Hit
GCAGGAGGGGCTGATTCTAAAGATAGTTCAAAGTAGGCACTAGAGGTAGG10340.10214817412608483No Hit
GCAGGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGC10130.10007359805582584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA207.856034E-444.00000422
CAAAACG207.856034E-444.00000438
CGAAACG207.856034E-444.00000420
CGACGAG408.310963E-944.00000415
TAGCGTT207.856034E-444.00000423
TAGCGTA207.856034E-444.00000419
CAATTGG207.856034E-444.00000424
GAATACG207.856034E-444.00000420
CGACACG207.856034E-444.00000428
AGCGGTA207.856034E-444.00000438
TACGCGA207.856034E-444.00000411
ACGGCTA207.856034E-444.00000415
CTTATCG207.856034E-444.00000428
CTACGAT207.856034E-444.00000413
TACGACG207.856034E-444.00000422
CGCTAAT207.856034E-444.00000413
CGTACTA207.856034E-444.00000433
CATACGC207.856034E-444.00000416
CTCGCAT207.856034E-444.00000441
ATTGGTA207.856034E-444.00000442
Produced by FastQC (version 0.11.5)