FastQCFastQC Report
Fri 17 Jun 2016
SRR1527281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091403
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC67350.6170956099625894No Hit
GCAGGAGGGATCAAGCAAGAGTACAATATGAAGATTCTTTTCTTGGCCCTTT39620.3630189764917267No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA37710.3455185664690311No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36110.33085853713064745No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT34020.3117088738073837No Hit
GCAGGAGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTT26040.23859197748219493No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT23180.21238717503983406No Hit
GCAGGAGGGGGTTAGGCTGGCCAGTACTTCTGGGTCTTGAGAACTGAGCCAA21210.1943370139169491No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC16760.1535638073195694No Hit
GCAGGAGGGATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGATTGG15280.14000328018156447No Hit
GCAGGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG14980.1372545246806175No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA14500.1328565158791024No Hit
GCAGGAGGGACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACC13910.1274506300605734No Hit
GCAGGAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT13270.12158661832521991No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT12510.11462310438948767No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT12170.11150784815508112No Hit
GCAGGAGAGGATGTTTCTGTGGGCCCCTTCACCCCTCCTCTGTCTGCACCTC11650.10674333862010642No Hit
GCAGGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTTTA11640.10665171343674151No Hit
GCAGGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG11620.10646846307001172No Hit
GCAGGAGGGATATTAAGGAAACAAACAAACTCATCTTACTGTGTGCTATTTT11450.10491083495280844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT551.8189894E-1246.00000417
CGTCATA551.8189894E-1246.00000436
TATTTCG206.311314E-446.033
AGCGTTG206.311314E-446.024
CTTCGCA206.311314E-446.030
AATCCGG301.8613155E-646.042
TCGCATC301.8613155E-646.042
GATACGA253.4170414E-546.026
CGTATTA253.4170414E-546.022
CGTATAC206.311314E-446.030
TACCACG301.8613155E-646.036
CAAAACG206.311314E-446.038
ACGCATG301.8613155E-646.031
CTATCGC253.4170414E-546.025
GCGCGTA253.4170414E-546.030
TAGCGTT206.311314E-446.023
ACCGATA253.4170414E-546.030
ACCGAAA206.311314E-446.044
TTCGTTA253.4170414E-546.028
CATTCGG206.311314E-446.031
Produced by FastQC (version 0.11.5)