##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1091403
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21372032145779	33.0	31.0	34.0	31.0	34.0
2	32.57684008565122	34.0	31.0	34.0	31.0	34.0
3	32.73208521508554	34.0	31.0	34.0	31.0	34.0
4	36.33374839541398	37.0	37.0	37.0	35.0	37.0
5	36.34061845166268	37.0	37.0	37.0	35.0	37.0
6	35.326597049852346	37.0	35.0	37.0	33.0	37.0
7	36.290537042687255	37.0	35.0	37.0	35.0	37.0
8	36.563563596581645	37.0	37.0	37.0	35.0	37.0
9	38.66501558086243	39.0	39.0	39.0	38.0	39.0
10	37.94722847564098	39.0	38.0	39.0	35.0	39.0
11	37.55651761998089	39.0	37.0	39.0	35.0	39.0
12	36.97317031380709	39.0	35.0	39.0	34.0	39.0
13	36.90745673229779	39.0	37.0	39.0	33.0	39.0
14	37.61572031596028	40.0	37.0	40.0	33.0	40.0
15	37.53144896981225	40.0	37.0	40.0	33.0	40.0
16	32.59475555775456	39.0	33.0	40.0	3.0	40.0
17	34.182171938321595	39.0	33.0	40.0	17.0	40.0
18	36.15474393968131	39.0	34.0	40.0	30.0	40.0
19	37.05973137328741	39.0	36.0	40.0	32.0	40.0
20	37.34806941157391	39.0	36.0	40.0	33.0	40.0
21	37.510020588178705	40.0	36.0	40.0	33.0	40.0
22	37.565026850759985	40.0	36.0	40.0	33.0	40.0
23	37.588582769151266	40.0	36.0	40.0	33.0	40.0
24	37.54972911014538	40.0	36.0	40.0	33.0	40.0
25	37.50183937555605	40.0	36.0	40.0	33.0	40.0
26	37.39327177953515	40.0	36.0	40.0	33.0	40.0
27	37.261076797479944	39.0	35.0	40.0	33.0	40.0
28	37.14816433526388	39.0	35.0	40.0	33.0	40.0
29	37.01950516903472	39.0	35.0	40.0	32.0	40.0
30	36.97282122185847	39.0	35.0	40.0	32.0	40.0
31	36.90785438559359	39.0	35.0	40.0	32.0	40.0
32	36.833347535236754	39.0	35.0	40.0	32.0	40.0
33	36.534144582706844	39.0	35.0	40.0	31.0	40.0
34	36.33643209703473	38.0	35.0	40.0	30.0	40.0
35	36.18714901828197	38.0	35.0	40.0	30.0	40.0
36	36.14869484507556	38.0	35.0	40.0	30.0	40.0
37	36.05996685000866	38.0	35.0	40.0	30.0	40.0
38	36.019047043117894	38.0	35.0	40.0	30.0	40.0
39	35.64574313979346	38.0	34.0	40.0	29.0	40.0
40	35.707125598885106	38.0	34.0	40.0	29.0	40.0
41	35.62798801176101	38.0	34.0	40.0	29.0	40.0
42	35.54016527350576	38.0	34.0	40.0	29.0	40.0
43	35.479633096115734	38.0	34.0	40.0	29.0	40.0
44	35.864254542089405	38.0	35.0	40.0	30.0	40.0
45	35.976506386733405	38.0	35.0	40.0	30.0	40.0
46	35.92638008141814	38.0	35.0	40.0	31.0	40.0
47	35.633557906657764	37.0	35.0	40.0	30.0	40.0
48	35.58848381395323	37.0	35.0	40.0	30.0	40.0
49	35.52774914490798	37.0	34.0	40.0	30.0	40.0
50	35.03292001213117	37.0	34.0	40.0	28.0	40.0
51	35.11459561683448	36.0	34.0	40.0	28.0	40.0
52	34.81038351553001	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	2.0
15	10.0
16	29.0
17	69.0
18	134.0
19	269.0
20	544.0
21	918.0
22	1479.0
23	2280.0
24	3430.0
25	4795.0
26	6841.0
27	9649.0
28	12657.0
29	16966.0
30	21925.0
31	28666.0
32	39251.0
33	56054.0
34	86590.0
35	92284.0
36	124635.0
37	195453.0
38	286617.0
39	99852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.55030360004507	0.35330670705504746	0.03829932664652745	0.05809036625334547
8	99.3317775377198	0.5110852728094023	0.1028034557354158	0.054333733735384644
9	98.05058259872843	1.3559610886171285	0.29649909336881064	0.29695721928563507
10	59.22642690188684	31.96491121977858	3.268361915809284	5.5402999625252995
11	35.64879334214768	24.921866624885585	22.55024037866856	16.879099654298184
12	31.691868173351185	16.793796608585463	28.25299179129982	23.261343426763535
13	27.259316677707503	16.39247830544721	30.7273298680689	25.62087514877639
14	24.620328146431703	20.545847867378043	31.98534363566895	22.848480350521303
15	25.61711851625843	21.266938060459793	31.435867411029655	21.68007601225212
16	28.60684824945506	29.04518312667273	23.75776867023455	18.590199953637658
17	30.640927320155797	21.333091442849252	24.966396464000923	23.059584772994025
18	27.921400252702256	21.562062776078132	27.204341567688562	23.312195403531053
19	28.40857135265342	22.341609836146684	26.473447479986767	22.776371331213127
20	27.717076093798532	23.182820644619813	26.698937056247786	22.40116620533387
21	27.719733224116116	22.151945706581344	28.220922977122108	21.907398092180433
22	26.342606718141692	21.310368397374756	28.72449498489559	23.622529899587963
23	27.490578640520503	21.65222195650919	28.598785233318946	22.25841416965136
24	26.901337086300842	22.441114785280963	27.153397965737682	23.504150162680514
25	28.076063562222203	23.98820600639727	23.45549719031375	24.480233241066774
26	26.09256159273889	23.01734556346281	25.81090577907519	25.07918706472311
27	23.896672448215735	22.210860699484975	26.150102207892047	27.742364644407246
28	24.39749570048827	24.127476285111914	25.407480096719542	26.067547917680272
29	27.41920262267925	22.861766002109214	25.14653157449631	24.572499800715224
30	27.9207588764187	21.380278412282173	26.270406073650154	24.42855663764897
31	27.261698932474985	23.417014613300495	25.47592410869312	23.84536234553139
32	29.19462380074088	22.3045016368839	25.518071693040977	22.982802869334243
33	31.100702490280856	21.67970951151866	25.806141269540216	21.413446728660265
34	27.55343351630882	23.123722401349454	28.00679492359834	21.31604915874338
35	25.106033243449026	25.276456084507736	28.93083489783334	20.686675774209892
36	24.88869830850749	26.750613659665586	27.01596019069033	21.344727841136592
37	27.241449766951348	26.042534242621652	24.73156111903669	21.98445487139031
38	25.979129615733143	25.932034271483584	25.824832806946656	22.264003305836617
39	27.066720542274485	25.42223175124129	25.775080332379513	21.73596737410471
40	26.46785834380151	24.875870782836405	24.882742671588773	23.773528201773313
41	24.504605539841837	25.384298925328224	25.36606551383861	24.74503002099133
42	24.376880034231167	24.59760510095721	25.1024598612978	25.923055003513827
43	23.186577277137776	23.772703575123032	26.5080817992987	26.532637348440492
44	23.95192243378477	22.921872122396586	26.438171784391283	26.68803365942736
45	24.97601710825424	23.117858389614103	26.36047362889785	25.54565087323381
46	25.22798636250771	25.409770726303666	24.834456199955472	24.52778671123316
47	24.03072009147858	25.45439219060237	25.87880003994858	24.636087677970465
48	24.679518014885428	24.43322952200058	25.80623289472358	25.08101956839041
49	25.79853637932093	23.53621897685823	26.032272222084785	24.63297242173606
50	24.579921440567784	24.252727910771732	27.082296823446516	24.08505382521397
51	23.410142724548127	24.787360855705913	27.51870757181353	24.283788847932435
52	22.663305854940845	25.081111193573776	27.698567806758824	24.557015144726556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	3.0
10	4.0
11	24.5
12	45.0
13	203.0
14	890.0
15	1419.0
16	2258.5
17	3098.0
18	3225.0
19	3352.0
20	3067.5
21	2783.0
22	3303.0
23	3823.0
24	4555.0
25	5287.0
26	5320.0
27	5353.0
28	5822.5
29	6292.0
30	7576.5
31	8861.0
32	10323.0
33	11785.0
34	13858.0
35	15931.0
36	18008.0
37	20085.0
38	22589.0
39	26478.0
40	27863.0
41	31942.5
42	36022.0
43	37884.0
44	39746.0
45	40335.5
46	40925.0
47	44794.5
48	48664.0
49	49916.0
50	51168.0
51	50918.5
52	50669.0
53	54789.5
54	58910.0
55	60044.5
56	61179.0
57	66287.5
58	71396.0
59	78622.0
60	85848.0
61	88669.0
62	91490.0
63	85076.0
64	75343.5
65	72025.0
66	65685.5
67	59346.0
68	47346.0
69	35346.0
70	27851.5
71	20357.0
72	19442.0
73	18527.0
74	15663.0
75	12799.0
76	11128.5
77	9458.0
78	6780.0
79	4102.0
80	3246.5
81	2391.0
82	1504.5
83	618.0
84	448.5
85	279.0
86	147.5
87	16.0
88	17.0
89	9.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1091403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.107377980638313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16305636675241	8.494906437091029
2	10.121185362094288	2.4508203358195835
3	4.166291596912631	1.5132860142413527
4	2.2639811019749523	1.096434997705312
5	1.309135668298273	0.7925100182011369
6	0.8744743995909389	0.6352555254143547
7	0.698149528969885	0.5916932163970098
8	0.5057541959992139	0.4898685773005053
9	0.43069366908332124	0.46931139409137557
>10	5.243272361189152	15.739967184462959
>50	2.3550942491013362	20.35823158492671
>100	1.789965426631948	38.002284656206626
>500	0.061749898997346675	4.958675438617538
>1k	0.016414530113218737	3.769376924635696
>5k	7.816442911056543E-4	0.6373776948888127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6735	0.6170956099625894	No Hit
GCAGGAGGGATCAAGCAAGAGTACAATATGAAGATTCTTTTCTTGGCCCTTT	3962	0.3630189764917267	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3771	0.3455185664690311	No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3611	0.33085853713064745	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3402	0.3117088738073837	No Hit
GCAGGAGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTT	2604	0.23859197748219493	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2318	0.21238717503983406	No Hit
GCAGGAGGGGGTTAGGCTGGCCAGTACTTCTGGGTCTTGAGAACTGAGCCAA	2121	0.1943370139169491	No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	1676	0.1535638073195694	No Hit
GCAGGAGGGATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGATTGG	1528	0.14000328018156447	No Hit
GCAGGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1498	0.1372545246806175	No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1450	0.1328565158791024	No Hit
GCAGGAGGGACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACC	1391	0.1274506300605734	No Hit
GCAGGAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT	1327	0.12158661832521991	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT	1251	0.11462310438948767	No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1217	0.11150784815508112	No Hit
GCAGGAGAGGATGTTTCTGTGGGCCCCTTCACCCCTCCTCTGTCTGCACCTC	1165	0.10674333862010642	No Hit
GCAGGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTTTA	1164	0.10665171343674151	No Hit
GCAGGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	1162	0.10646846307001172	No Hit
GCAGGAGGGATATTAAGGAAACAAACAAACTCATCTTACTGTGTGCTATTTT	1145	0.10491083495280844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.006505388018907773	0.0	0.0	0.0	0.0
7	0.006505388018907773	0.0	0.0	0.0	0.0
8	0.006688638385637569	0.0	0.0	0.0	0.0
9	0.0071467643024620605	0.0	0.0	0.0	0.0
10	0.010903396820422887	0.0	0.0	0.0	0.0
11	0.01951616405672332	0.0	0.0	0.0	0.0
12	0.024922049875252313	0.0	0.0	0.0	0.0
13	0.028587057209848242	0.0	0.0	0.0	0.0
14	0.03655844816259438	0.0	0.0	0.0	0.0
15	0.044346588748610735	0.0	0.0	0.0	0.0
16	0.058181991436710365	0.0	0.0	0.0	0.0
17	0.06422925353879365	0.0	0.0	0.0	0.0
18	0.07027651564087693	0.0	0.0	0.0	0.0
19	0.08008041026092104	0.0	0.0	0.0	0.0
20	0.0865857982798288	0.0	0.0	0.0	0.0
21	0.09428231368248026	0.0	0.0	0.0	0.0
22	0.10124582761821252	0.0	0.0	0.0	0.0
23	0.10692658898683621	0.0	0.0	0.0	0.0
24	0.11553935622313664	0.0	0.0	0.0	0.0
25	0.11947923910782726	0.0	0.0	0.0	0.0
26	0.12415212345943707	0.0	0.0	0.0	0.0
27	0.13249001514564282	0.0	0.0	0.0	0.0
28	0.14091953201521346	0.0	0.0	0.0	0.0
29	0.14623379265037753	0.0	0.0	0.0	0.0
30	0.15108992736871715	0.0	0.0	0.0	0.0
31	0.1592445686881931	0.0	0.0	0.0	0.0
32	0.16410070340653268	0.0	0.0	0.0	0.0
33	0.1691400884916021	0.0	0.0	0.0	0.0
34	0.17784448091126742	0.0	0.0	0.0	0.0
35	0.1886562525483254	0.0	0.0	0.0	0.0
36	0.20203352931960056	0.0	0.0	0.0	0.0
37	0.209730044722252	0.0	0.0	0.0	0.0
38	0.21632705792452467	0.0	0.0	0.0	0.0
39	0.22246594520997284	0.0	0.0	0.0	0.0
40	0.23199496427992228	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	55	1.8189894E-12	46.000004	17
CGTCATA	55	1.8189894E-12	46.000004	36
TATTTCG	20	6.311314E-4	46.0	33
AGCGTTG	20	6.311314E-4	46.0	24
CTTCGCA	20	6.311314E-4	46.0	30
AATCCGG	30	1.8613155E-6	46.0	42
TCGCATC	30	1.8613155E-6	46.0	42
GATACGA	25	3.4170414E-5	46.0	26
CGTATTA	25	3.4170414E-5	46.0	22
CGTATAC	20	6.311314E-4	46.0	30
TACCACG	30	1.8613155E-6	46.0	36
CAAAACG	20	6.311314E-4	46.0	38
ACGCATG	30	1.8613155E-6	46.0	31
CTATCGC	25	3.4170414E-5	46.0	25
GCGCGTA	25	3.4170414E-5	46.0	30
TAGCGTT	20	6.311314E-4	46.0	23
ACCGATA	25	3.4170414E-5	46.0	30
ACCGAAA	20	6.311314E-4	46.0	44
TTCGTTA	25	3.4170414E-5	46.0	28
CATTCGG	20	6.311314E-4	46.0	31
>>END_MODULE