FastQCFastQC Report
Fri 17 Jun 2016
SRR1527280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061161
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC64500.6078248258275606No Hit
GCAGGAGGGATCAAGCAAGAGTACAATATGAAGATTCTTTTCTTGGCCCTTT40880.3852384322454368No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37300.3515017985018296No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA36420.3432089946765854No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT34610.3261522049905716No Hit
GCAGGAGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTT24060.22673279549474584No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT23610.22249215717501866No Hit
GCAGGAGGGGGTTAGGCTGGCCAGTACTTCTGGGTCTTGAGAACTGAGCCAA21640.20392758497532418No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC16320.1537938163954386No Hit
GCAGGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG15870.14955317807571142No Hit
GCAGGAGGGATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGATTGG14380.13551195341705924No Hit
GCAGGAGGGACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACC13480.1270306767776049No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT13160.12401511175024336No Hit
GCAGGAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT12380.1166646719960496No Hit
GCAGGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG12320.11609925355341932No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT11880.1119528516407972No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA11750.11072777834843156No Hit
GCAGGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTTTA11680.11006812349869624No Hit
GCAGGAGAGGATGTTTCTGTGGGCCCCTTCACCCCTCCTCTGTCTGCACCTC11480.10818339535659527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG351.0194708E-746.00000425
CGACTAA351.0194708E-746.00000424
TCGCATT206.3112355E-446.027
TACCACG253.4169778E-546.036
CAACGGA253.4169778E-546.046
CGAAACG405.6115823E-946.020
ACGTTAA253.4169778E-546.013
TAGCGTC206.3112355E-446.028
TAGCGCG206.3112355E-446.024
ATTCGGC206.3112355E-446.017
CGTCTCG600.046.042
ATTCGCG253.4169778E-546.033
GATCACG206.3112355E-446.09
TAACGAA206.3112355E-446.039
GTAATCG301.8612664E-646.043
ATATCGC301.8612664E-646.023
GTAGCCG301.8612664E-646.043
CACTCGA206.3112355E-446.036
CCGTAAT453.092282E-1046.028
TACGCAC253.4169778E-546.039
Produced by FastQC (version 0.11.5)