##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1061161
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20616287255186	33.0	31.0	34.0	31.0	34.0
2	32.58412625416878	34.0	31.0	34.0	31.0	34.0
3	32.71159795733164	34.0	31.0	34.0	31.0	34.0
4	36.32940901522012	37.0	37.0	37.0	35.0	37.0
5	36.35245452857766	37.0	37.0	37.0	35.0	37.0
6	35.3148297006769	37.0	35.0	37.0	33.0	37.0
7	36.29274916812812	37.0	35.0	37.0	35.0	37.0
8	36.58573863909435	37.0	37.0	37.0	35.0	37.0
9	38.603985634602104	39.0	39.0	39.0	38.0	39.0
10	37.93211020759338	39.0	38.0	39.0	35.0	39.0
11	37.48199189378426	39.0	37.0	39.0	35.0	39.0
12	36.917840930829534	39.0	35.0	39.0	33.0	39.0
13	36.80156357046668	39.0	35.0	39.0	33.0	39.0
14	37.52520776771856	40.0	37.0	40.0	33.0	40.0
15	37.63015131539889	40.0	37.0	40.0	33.0	40.0
16	37.754637609184655	40.0	37.0	40.0	33.0	40.0
17	37.684009306787566	40.0	37.0	40.0	33.0	40.0
18	37.67482031473075	40.0	37.0	40.0	33.0	40.0
19	37.64973646788753	40.0	37.0	40.0	33.0	40.0
20	37.612083369064635	40.0	37.0	40.0	33.0	40.0
21	33.338578217631444	39.0	33.0	40.0	10.0	40.0
22	34.01423063983693	39.0	33.0	40.0	16.0	40.0
23	36.090548936495026	39.0	34.0	40.0	30.0	40.0
24	36.88959733725608	39.0	35.0	40.0	32.0	40.0
25	37.19167685205167	39.0	35.0	40.0	33.0	40.0
26	37.26310427918101	39.0	35.0	40.0	33.0	40.0
27	37.2129224500335	39.0	35.0	40.0	33.0	40.0
28	37.1179877511518	39.0	35.0	40.0	33.0	40.0
29	37.162233629015766	39.0	35.0	40.0	33.0	40.0
30	37.040056127204075	39.0	35.0	40.0	32.0	40.0
31	36.87602540990481	39.0	35.0	40.0	32.0	40.0
32	36.83887270640365	39.0	35.0	40.0	32.0	40.0
33	36.54986566600167	39.0	35.0	40.0	31.0	40.0
34	36.344808186505155	38.0	35.0	40.0	30.0	40.0
35	36.194902564266876	38.0	35.0	40.0	30.0	40.0
36	36.09409882195067	38.0	35.0	40.0	30.0	40.0
37	36.220572561562285	38.0	35.0	40.0	31.0	40.0
38	36.10865551975619	38.0	35.0	40.0	30.0	40.0
39	35.95018003865577	38.0	35.0	40.0	30.0	40.0
40	31.21836554490789	36.0	30.0	40.0	9.0	40.0
41	32.05017334787087	36.0	30.0	40.0	13.0	40.0
42	33.776416585230706	36.0	30.0	40.0	23.0	40.0
43	34.698570716413435	36.0	33.0	40.0	27.0	40.0
44	35.545340433732484	37.0	34.0	40.0	30.0	40.0
45	35.83771454096033	38.0	35.0	40.0	30.0	40.0
46	35.88331365363032	38.0	35.0	40.0	31.0	40.0
47	35.84246594060656	38.0	35.0	40.0	31.0	40.0
48	35.710157082667	37.0	35.0	40.0	30.0	40.0
49	35.59151721557803	37.0	35.0	40.0	30.0	40.0
50	35.48212382475421	37.0	34.0	40.0	30.0	40.0
51	35.31756632593923	37.0	34.0	40.0	29.0	40.0
52	34.956576805970066	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	10.0
16	22.0
17	72.0
18	153.0
19	333.0
20	588.0
21	1018.0
22	1586.0
23	2416.0
24	3641.0
25	5107.0
26	7313.0
27	9875.0
28	13542.0
29	17956.0
30	23489.0
31	32061.0
32	43457.0
33	51976.0
34	95561.0
35	109043.0
36	126670.0
37	133620.0
38	277536.0
39	104112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.55303672110075	0.35159603490893465	0.03778879924912431	0.05757844474118442
8	99.34590509828386	0.5183002390777648	0.08198567418139188	0.05380898845698249
9	98.01679481247426	1.344282347353512	0.33651820977212693	0.3024046304000995
10	59.32521078328359	31.845780235044447	3.2815001682119864	5.547508813459975
11	35.774307574439696	24.841470804147534	22.54606039988277	16.838161221530004
12	31.775008693308553	16.75523318327756	28.26357169176025	23.206186431653634
13	27.43975702084792	16.34210077452903	30.54399850729531	25.67414369732774
14	24.645930259404558	20.54900246051259	32.00635907275145	22.7987082073314
15	25.630889186466522	21.086338453825572	31.475713864342925	21.80705849536498
16	30.134258609202565	21.330316511820545	26.933801751100916	21.60162312787598
17	30.627491963990384	21.40155923559196	24.910828799776848	23.060120000640808
18	27.96418262638751	21.548191085047417	27.220563137921577	23.267063150643494
19	28.51235580651758	22.292658701177295	26.403533488320814	22.79145200398432
20	27.800493987246043	23.20279392099785	26.59671812288616	22.399993968869943
21	32.48065090971116	21.30072627998956	26.42784648135391	19.790776328945373
22	26.54743248197022	21.296485641669832	28.67067297045406	23.485408905905892
23	27.576682520371556	21.69124195103288	28.503120638621283	22.228954889974283
24	27.102013737783427	22.42751099974462	27.102673392633164	23.36780186983879
25	28.09960034339747	23.995133631937097	23.346504441833048	24.55876158283239
26	26.165209614752143	22.980301763822833	25.783363693162485	25.071124928262535
27	23.89373525789206	22.172413045711252	26.115358555393577	27.81849314100311
28	24.40100983733854	24.1277242567339	25.35072434814321	26.12054155778435
29	27.253922826036764	22.837816316279998	25.14217917921974	24.766081678463493
30	27.950424110950177	21.337478478760527	26.255299619944573	24.456797790344726
31	27.320924911488454	23.458080347845424	25.497073488377353	23.723921252288765
32	29.262571843480867	22.24459813355372	25.429317511668824	23.063512511296587
33	31.13797058127843	21.67390245212555	25.808053631824013	21.38007333477201
34	27.69193364626103	23.071428369493415	27.878710205143236	21.357927779102322
35	25.088275954355655	25.356943951012145	28.906075515402467	20.64870457922973
36	25.011002100529517	26.82599530137274	26.89544753340916	21.26755506468858
37	27.320453729452932	25.995866791184376	24.663835176754517	22.01984430260818
38	26.05127779856214	25.97334428988627	25.716832789746324	22.258545121805266
39	26.87716567043078	25.401706244387046	25.81220003373663	21.90892805144554
40	27.111720087715245	29.062131005568432	22.577158414227437	21.248990492488886
41	24.488178513910707	25.39624053277495	25.357320896640566	24.758260056673777
42	24.321097364113456	24.614455299431473	25.03277071057078	26.03167662588429
43	23.134001343811164	23.774431966497072	26.561190997407557	26.53037569228421
44	23.8962796408839	22.934785579191093	26.36338877889406	26.805546001030944
45	24.991118218630348	23.213348398593617	26.315045502049173	25.480487880726866
46	25.193443784684888	25.37984339793867	24.801608803942095	24.625104013434342
47	23.852271238765844	25.511114713036005	25.84160179275341	24.795012255444743
48	24.690692552779456	24.41081042367746	25.787604331482218	25.110892692060865
49	25.709576586399237	23.567206107273073	25.98559502280992	24.73762228351777
50	24.51060677880171	24.261822664044384	27.13179244242862	24.095778114725285
51	23.41001978022185	24.887458170814796	27.481786458416774	24.220735590546578
52	22.667154183012755	25.089124082019598	27.707953835468885	24.535767899498754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	36.5
12	71.0
13	199.5
14	850.0
15	1372.0
16	2141.0
17	2910.0
18	3045.5
19	3181.0
20	2880.0
21	2579.0
22	3044.5
23	3510.0
24	4222.5
25	4935.0
26	5049.0
27	5163.0
28	5720.0
29	6277.0
30	7324.0
31	8371.0
32	9738.5
33	11106.0
34	13225.0
35	15344.0
36	17355.5
37	19367.0
38	21588.5
39	24924.5
40	26039.0
41	30598.5
42	35158.0
43	36902.5
44	38647.0
45	38992.5
46	39338.0
47	42897.0
48	46456.0
49	47981.5
50	49507.0
51	49219.0
52	48931.0
53	53049.0
54	57167.0
55	58563.5
56	59960.0
57	64508.0
58	69056.0
59	76229.0
60	83402.0
61	86413.0
62	89424.0
63	82963.0
64	73626.5
65	70751.0
66	64945.0
67	59139.0
68	47339.5
69	35540.0
70	27883.0
71	20226.0
72	19171.5
73	18117.0
74	15316.5
75	12516.0
76	11050.5
77	9585.0
78	6840.5
79	4096.0
80	3247.0
81	2398.0
82	1501.5
83	605.0
84	420.0
85	235.0
86	122.0
87	9.0
88	15.5
89	12.5
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1061161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.362697706912625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.27271226151066	8.193095079062584
2	11.056776857336745	2.7338317980008315
3	5.343230993652288	1.9817024865818882
4	3.0685432943049866	1.5174189259226551
5	1.8665951751576588	1.1538075945827884
6	1.2857542419228523	0.9537234610963696
7	0.8805008471651771	0.7619756062928491
8	0.7039982567452016	0.6962654107467511
9	0.49565536983737796	0.5514873753696734
>10	4.964468798924541	15.364668991495073
>50	2.264339594039369	20.013712901998336
>100	1.725591188689854	37.11274323528332
>500	0.05507205921350597	4.541394943127118
>1k	0.0159629157140597	3.787735528122122
>5k	7.981457857029851E-4	0.6364366623176515
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6450	0.6078248258275606	No Hit
GCAGGAGGGATCAAGCAAGAGTACAATATGAAGATTCTTTTCTTGGCCCTTT	4088	0.3852384322454368	No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3730	0.3515017985018296	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3642	0.3432089946765854	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3461	0.3261522049905716	No Hit
GCAGGAGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTT	2406	0.22673279549474584	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2361	0.22249215717501866	No Hit
GCAGGAGGGGGTTAGGCTGGCCAGTACTTCTGGGTCTTGAGAACTGAGCCAA	2164	0.20392758497532418	No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	1632	0.1537938163954386	No Hit
GCAGGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1587	0.14955317807571142	No Hit
GCAGGAGGGATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGATTGG	1438	0.13551195341705924	No Hit
GCAGGAGGGACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACC	1348	0.1270306767776049	No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1316	0.12401511175024336	No Hit
GCAGGAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT	1238	0.1166646719960496	No Hit
GCAGGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	1232	0.11609925355341932	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT	1188	0.1119528516407972	No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1175	0.11072777834843156	No Hit
GCAGGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTTTA	1168	0.11006812349869624	No Hit
GCAGGAGAGGATGTTTCTGTGGGCCCCTTCACCCCTCCTCTGTCTGCACCTC	1148	0.10818339535659527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.006031130054723082	0.0	0.0	0.0	0.0
7	0.006031130054723082	0.0	0.0	0.0	0.0
8	0.006219602868933178	0.0	0.0	0.0	0.0
9	0.006408075683143274	0.0	0.0	0.0	0.0
10	0.01027176837445025	0.0	0.0	0.0	0.0
11	0.019506936270744967	0.0	0.0	0.0	0.0
12	0.02591501195388824	0.0	0.0	0.0	0.0
13	0.028930576981249782	0.0	0.0	0.0	0.0
14	0.037411853620704115	0.0	0.0	0.0	0.0
15	0.04655278510989379	0.0	0.0	0.0	0.0
16	0.05833233599802481	0.0	0.0	0.0	0.0
17	0.06379804761011759	0.0	0.0	0.0	0.0
18	0.0712427237714164	0.0	0.0	0.0	0.0
19	0.08047789166771112	0.0	0.0	0.0	0.0
20	0.08556665765138372	0.0	0.0	0.0	0.0
21	0.09093813285637146	0.0	0.0	0.0	0.0
22	0.09762891776082988	0.0	0.0	0.0	0.0
23	0.10300039296581763	0.0	0.0	0.0	0.0
24	0.11129319679106187	0.0	0.0	0.0	0.0
25	0.11666467199604962	0.0	0.0	0.0	0.0
26	0.12194191079393231	0.0	0.0	0.0	0.0
27	0.1301404782120715	0.0	0.0	0.0	0.0
28	0.13946988251547127	0.0	0.0	0.0	0.0
29	0.1452183033488792	0.0	0.0	0.0	0.0
30	0.15021283292544674	0.0	0.0	0.0	0.0
31	0.15605549016595974	0.0	0.0	0.0	0.0
32	0.16199238381357778	0.0	0.0	0.0	0.0
33	0.16877740512514125	0.0	0.0	0.0	0.0
34	0.17499700799407442	0.0	0.0	0.0	0.0
35	0.18677655888220543	0.0	0.0	0.0	0.0
36	0.20091201994796265	0.0	0.0	0.0	0.0
37	0.20750856844531604	0.0	0.0	0.0	0.0
38	0.21344546209293405	0.0	0.0	0.0	0.0
39	0.2209843746613379	0.0	0.0	0.0	0.0
40	0.2291829420794771	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATTCG	35	1.0194708E-7	46.000004	25
CGACTAA	35	1.0194708E-7	46.000004	24
TCGCATT	20	6.3112355E-4	46.0	27
TACCACG	25	3.4169778E-5	46.0	36
CAACGGA	25	3.4169778E-5	46.0	46
CGAAACG	40	5.6115823E-9	46.0	20
ACGTTAA	25	3.4169778E-5	46.0	13
TAGCGTC	20	6.3112355E-4	46.0	28
TAGCGCG	20	6.3112355E-4	46.0	24
ATTCGGC	20	6.3112355E-4	46.0	17
CGTCTCG	60	0.0	46.0	42
ATTCGCG	25	3.4169778E-5	46.0	33
GATCACG	20	6.3112355E-4	46.0	9
TAACGAA	20	6.3112355E-4	46.0	39
GTAATCG	30	1.8612664E-6	46.0	43
ATATCGC	30	1.8612664E-6	46.0	23
GTAGCCG	30	1.8612664E-6	46.0	43
CACTCGA	20	6.3112355E-4	46.0	36
CCGTAAT	45	3.092282E-10	46.0	28
TACGCAC	25	3.4169778E-5	46.0	39
>>END_MODULE