FastQCFastQC Report
Fri 17 Jun 2016
SRR1527279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762020
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC63620.36106287102303036No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC56830.3225275536032508No Hit
AGATGGGGGAGTTAACTTTGCATCTTCATACTCCTCAAGCAGACATCACT51030.28961078761875575No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG45820.260042451277511No Hit
AGATGGGGGAACAACAAAACACTATTGTTTTCTTTTCTACAGCATTAGAG45640.25902089647109566No Hit
AGATGGGGGAATTCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCT44080.25016742148216253No Hit
AGATGGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA24300.13790989886607416No Hit
AGATGGGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCG23020.1306455091315649No Hit
AGATGGGGGTGTGTGGTTCCTCAGCGATGTAATCTTGTTTAAATGTAAAG20890.1185571105889831No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT20810.11810308623057626No Hit
AGATGGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG20720.11759230882736858No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC19780.11225752261608835No Hit
AGATGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT18860.10703624249440982No Hit
AGATGGGGGAGAGTAAGTTTTGAATTTAGGATGTGTTAGTTCTCTAGCTT18560.1053336511503842No Hit
AGATGGGGGACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGTCTA18360.10419859025436716No Hit
AGATGGGGGCCAGGAGGAGTACATCAAGCGCGCCCTGGCCAACAGCCTCG18130.10289327022394751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.8576244E-444.00000412
CGCATTA207.8576244E-444.00000412
TACGCAA207.8576244E-444.00000434
CGAATTA408.3164196E-944.00000441
CCGAATT207.8576244E-444.00000444
ATACGAC207.8576244E-444.00000437
GACCTAA207.8576244E-444.0000049
AGCGTAA254.4438097E-544.030
CCGATAG454.802132E-1044.027
TAGACGC351.4466787E-744.027
CGTTCGA302.528488E-644.019
CCGTACG351.4466787E-744.034
TACGCGC351.4466787E-744.037
TATCTCG302.528488E-644.013
ATCGCGT551.8189894E-1244.019
GCGTAAT254.4438097E-544.031
CCGCAAT254.4438097E-544.019
ACGACGC254.4438097E-544.021
ACGAAAT254.4438097E-544.020
CGTAGAT850.043.99999632
Produced by FastQC (version 0.11.5)