##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762020
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23662330734044	33.0	31.0	34.0	31.0	34.0
2	32.70991532445716	34.0	31.0	34.0	31.0	34.0
3	32.160830751069795	33.0	31.0	34.0	31.0	34.0
4	36.36764906187217	37.0	37.0	37.0	35.0	37.0
5	36.34134686325921	37.0	37.0	37.0	35.0	37.0
6	36.38770672296569	37.0	37.0	37.0	35.0	37.0
7	36.64353866584942	37.0	37.0	37.0	35.0	37.0
8	36.67684021747767	37.0	37.0	37.0	35.0	37.0
9	38.55240519403866	39.0	39.0	39.0	37.0	39.0
10	36.39636780513275	37.0	35.0	39.0	33.0	39.0
11	35.887219781841296	37.0	35.0	39.0	32.0	39.0
12	35.79904938649958	37.0	35.0	39.0	32.0	39.0
13	35.88492752636179	37.0	35.0	39.0	32.0	39.0
14	36.560544715724	38.0	35.0	40.0	33.0	40.0
15	36.669321006572005	38.0	35.0	40.0	33.0	40.0
16	36.59321233584182	38.0	35.0	40.0	33.0	40.0
17	36.657305819457214	38.0	35.0	40.0	33.0	40.0
18	36.62358770048013	38.0	35.0	40.0	33.0	40.0
19	36.62408882986572	38.0	35.0	40.0	33.0	40.0
20	36.310189441663546	38.0	35.0	40.0	32.0	40.0
21	36.512903372265924	38.0	35.0	40.0	33.0	40.0
22	36.375963950465945	38.0	35.0	40.0	33.0	40.0
23	36.45761739367317	38.0	35.0	40.0	33.0	40.0
24	36.477038285604024	38.0	35.0	40.0	33.0	40.0
25	36.464683147750875	38.0	35.0	40.0	33.0	40.0
26	36.33066026492321	38.0	35.0	40.0	33.0	40.0
27	36.25548801943224	37.0	35.0	40.0	32.0	40.0
28	36.086378701717344	37.0	35.0	40.0	32.0	40.0
29	36.34324184742511	38.0	35.0	40.0	33.0	40.0
30	36.39083778844735	38.0	35.0	40.0	33.0	40.0
31	36.399748016481084	38.0	35.0	40.0	33.0	40.0
32	35.70104255343299	37.0	35.0	40.0	31.0	40.0
33	35.934842396794586	37.0	35.0	40.0	31.0	40.0
34	36.00335410494773	37.0	35.0	40.0	31.0	40.0
35	36.025088818515115	37.0	35.0	40.0	32.0	40.0
36	35.9010533365115	36.0	35.0	40.0	31.0	40.0
37	35.88459835870194	36.0	35.0	40.0	31.0	40.0
38	35.71191189657325	36.0	35.0	40.0	31.0	40.0
39	35.70148806483468	36.0	35.0	40.0	31.0	40.0
40	35.62331358327374	36.0	35.0	40.0	31.0	40.0
41	35.52374660900557	36.0	35.0	40.0	31.0	40.0
42	35.458551548790595	36.0	35.0	40.0	31.0	40.0
43	35.388259497622045	35.0	35.0	40.0	30.0	40.0
44	35.2647189021691	35.0	35.0	40.0	30.0	40.0
45	35.20458224083722	35.0	35.0	40.0	30.0	40.0
46	34.97179260167308	35.0	34.0	40.0	29.0	40.0
47	34.912967503206545	35.0	34.0	40.0	29.0	40.0
48	34.87018819309656	35.0	34.0	40.0	29.0	40.0
49	34.63583841273084	35.0	34.0	40.0	28.0	40.0
50	34.19341040396817	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	4.0
14	10.0
15	50.0
16	152.0
17	315.0
18	620.0
19	1008.0
20	1604.0
21	2506.0
22	3748.0
23	5025.0
24	6949.0
25	9082.0
26	12061.0
27	15918.0
28	20770.0
29	27313.0
30	35766.0
31	46062.0
32	62863.0
33	106727.0
34	287489.0
35	215506.0
36	88847.0
37	161094.0
38	415511.0
39	235016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.77379371403275	0.9935188022837426	0.16611616213209837	0.06657132155140123
8	97.48822374320383	1.7794349666859628	0.4599834281109182	0.2723578619992963
9	90.53580549596487	4.881499642455818	3.033393491560822	1.5493013700185014
10	42.82346397884246	41.30435522865802	6.718028172211439	9.154152620288079
11	30.377521254015278	22.694294048875722	26.51417123528677	20.41401346182223
12	28.070282970681376	16.876482673295424	29.594783260121904	25.458451095901296
13	24.35971214855677	18.288782193164664	30.550901805881885	26.80060385239668
14	23.8032485442844	20.379053586224902	31.649300234957607	24.168397634533093
15	24.00977287431471	22.77119442458088	28.052462514613907	25.166570186490507
16	29.433320847663474	22.256274049102736	25.28995130588756	23.020453797346228
17	28.736052939240192	21.048796267919773	24.63683726631934	25.578313526520695
18	26.50327465068501	21.529778322607008	26.194367827833965	25.77257919887402
19	27.968184243084643	21.35571673420279	25.708277999114653	24.967821023597917
20	26.984994494954655	21.782442878060408	27.285558620219973	23.947004006764963
21	27.40082405421051	20.57729197171428	27.568415795507427	24.453468178567782
22	26.801114629799887	21.322970227352698	26.97511946515931	24.90079567768811
23	25.67212630957651	22.423298259951647	27.361777959387524	24.542797471084324
24	25.92093165798345	23.291619845404707	25.472525851011905	25.314922645599935
25	26.737721478757337	23.02272391913826	26.029556985732285	24.209997616372117
26	26.452707687767447	23.173800524398132	25.363219486725463	25.010272301108955
27	25.878366874382813	21.32177841341188	25.625078035436598	27.17477667676871
28	26.07234878151213	21.80934382129601	25.232119953235493	26.886187443956366
29	26.51939251540845	22.318702398383675	25.536032508144064	25.625872578063813
30	26.85429223277829	21.130066628074594	26.578926459404546	25.43671467974257
31	26.79839048364945	22.951158329644386	25.784724350461403	24.465726836244762
32	29.288089806018093	22.10576497429087	24.725088250984665	23.88105696870637
33	28.165911851170815	21.979148931340166	25.78580265831262	24.0691365591764
34	26.696405262142314	22.987537031361732	27.305535691989874	23.01052201450608
35	25.483025164300066	24.194220269917484	28.078058137819095	22.24469642796336
36	25.889376965074177	23.64899376851568	26.198681059238826	24.262948207171313
37	26.7754055005051	24.752556724668278	24.15477690378089	24.317260871045733
38	27.31813486793567	24.70494092008036	25.263163868741557	22.713760343242413
39	27.090782170463445	23.067615577575737	24.51521549131111	25.32638676064971
40	25.464977696053392	23.441277624544558	25.597155537394585	25.49658914200747
41	24.43757732602354	25.405159986833294	25.14994154436386	25.00732114277931
42	24.978547349065277	23.826801057876757	25.405330245967694	25.789321347090272
43	24.7738391164686	23.478394115844313	27.08697971646179	24.6607870512253
44	25.337340098296274	23.260348917719437	26.491583523456036	24.910727460528257
45	26.39368452117456	22.788958127603546	25.63018580946868	25.187171541753216
46	25.332856607757005	23.91834371914053	25.641479665384047	25.107320007718414
47	24.208976061565703	23.54451141303731	26.558098091962634	25.688414433434353
48	25.98614090645963	24.028047354740583	24.711183755008456	25.27462798379133
49	25.268782420176844	24.569017377782316	24.769525885063732	25.392674316977104
50	24.527984926391298	24.972020748913177	25.638982531412815	24.86101179328271
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	97.0
8	191.0
9	302.5
10	414.0
11	700.0
12	986.0
13	2082.0
14	3178.0
15	3677.0
16	4176.0
17	4273.5
18	4371.0
19	5227.0
20	6083.0
21	5920.5
22	5758.0
23	6266.0
24	6774.0
25	8865.0
26	10956.0
27	11090.0
28	11224.0
29	12649.0
30	14074.0
31	20209.5
32	26345.0
33	26740.5
34	27136.0
35	30164.5
36	33193.0
37	36822.0
38	40451.0
39	43777.5
40	47104.0
41	57760.5
42	68417.0
43	72108.0
44	75799.0
45	75791.5
46	75784.0
47	78388.5
48	80993.0
49	87403.5
50	93814.0
51	99731.5
52	105649.0
53	106995.5
54	108342.0
55	119219.5
56	130097.0
57	135067.5
58	140038.0
59	139237.5
60	138437.0
61	133311.0
62	128185.0
63	121759.5
64	115334.0
65	101588.0
66	87842.0
67	72037.5
68	56233.0
69	47710.5
70	39188.0
71	35236.0
72	31284.0
73	24370.5
74	17457.0
75	16393.0
76	15329.0
77	11596.0
78	7863.0
79	5047.5
80	2232.0
81	1518.5
82	805.0
83	603.0
84	401.0
85	222.5
86	44.0
87	35.5
88	27.0
89	17.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1762020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.841645715541776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.81858716597127	5.161260420530514
2	10.748431964116369	1.6857079092041076
3	4.700146699334577	1.1057065568176438
4	2.5009106333836884	0.7844502061290426
5	1.4202864414099867	0.5568691544062347
6	0.8998084660130699	0.42335875213917634
7	0.5971369094838259	0.327777526149199
8	0.44992391873458953	0.2822515175731888
9	0.3345670912214298	0.23612009376940196
>10	5.261484735940852	11.573751029038554
>50	3.8909142004510984	22.001543722033396
>100	3.2301218391428854	44.45035615430725
>500	0.1100064820421883	5.856974897942795
>1k	0.035413045588923636	4.540693216735786
>5k	0.0022604071652504443	1.013178843223709
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6362	0.36106287102303036	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5683	0.3225275536032508	No Hit
AGATGGGGGAGTTAACTTTGCATCTTCATACTCCTCAAGCAGACATCACT	5103	0.28961078761875575	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4582	0.260042451277511	No Hit
AGATGGGGGAACAACAAAACACTATTGTTTTCTTTTCTACAGCATTAGAG	4564	0.25902089647109566	No Hit
AGATGGGGGAATTCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCT	4408	0.25016742148216253	No Hit
AGATGGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA	2430	0.13790989886607416	No Hit
AGATGGGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCG	2302	0.1306455091315649	No Hit
AGATGGGGGTGTGTGGTTCCTCAGCGATGTAATCTTGTTTAAATGTAAAG	2089	0.1185571105889831	No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2081	0.11810308623057626	No Hit
AGATGGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	2072	0.11759230882736858	No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1978	0.11225752261608835	No Hit
AGATGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1886	0.10703624249440982	No Hit
AGATGGGGGAGAGTAAGTTTTGAATTTAGGATGTGTTAGTTCTCTAGCTT	1856	0.1053336511503842	No Hit
AGATGGGGGACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGTCTA	1836	0.10419859025436716	No Hit
AGATGGGGGCCAGGAGGAGTACATCAAGCGCGCCCTGGCCAACAGCCTCG	1813	0.10289327022394751	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.1350608960170714E-4	0.0	0.0	0.0	0.0
8	0.001191813940817925	0.0	0.0	0.0	0.0
9	0.002497133971237557	0.0	0.0	0.0	0.0
10	0.008229191496123767	0.0	0.0	0.0	0.0
11	0.012542422900988639	0.0	0.0	0.0	0.0
12	0.01594760558903985	0.0	0.0	0.0	0.0034051826880512137
13	0.020487849173108138	0.0	0.0	0.0	0.0034051826880512137
14	0.02911431198283788	0.0	0.0	0.0	0.0034051826880512137
15	0.037513762613364206	0.0	0.0	0.0	0.0034051826880512137
16	0.047956322856721266	0.0	0.0	0.0	0.0034051826880512137
17	0.05522071259123052	0.0	0.0	0.0	0.0034051826880512137
18	0.06276886754974405	0.0	0.0	0.0	0.0034051826880512137
19	0.070146763373855	0.0	0.0	0.0	0.0034051826880512137
20	0.07655985743635146	0.0	0.0	0.0	0.0034051826880512137
21	0.08246217409564023	0.0	0.0	0.0	0.0034051826880512137
22	0.08870500902373413	0.0	0.0	0.0	0.0034051826880512137
23	0.0932452526078024	0.0	0.0	0.0	0.0034051826880512137
24	0.10090691365591764	0.0	0.0	0.0	0.0034051826880512137
25	0.10612819377759616	0.0	0.0	0.0	0.0034051826880512137
26	0.1113494738992747	0.0	0.0	0.0	0.0034051826880512137
27	0.11702477837936005	0.0	0.0	0.0	0.0034051826880512137
28	0.12235956459064029	0.0	0.0	0.0	0.0034051826880512137
29	0.12848889342913247	0.0	0.0	0.0	0.0034051826880512137
30	0.1346749753124255	0.0	0.0	0.0	0.0034051826880512137
31	0.13955573716529893	0.0	0.0	0.0	0.0034051826880512137
32	0.14613909036219794	0.0	0.0	0.0	0.0034051826880512137
33	0.15215491311108842	0.0	0.0	0.0	0.0034051826880512137
34	0.15788697063597462	0.0	0.0	0.0	0.0034051826880512137
35	0.1656053847288907	0.0	0.0	0.0	0.0034051826880512137
36	0.17235899706019228	0.0	0.0	0.0	0.0034051826880512137
37	0.1789423502570913	0.0	0.0	0.0	0.0034051826880512137
38	0.18478791387157922	0.0	0.0	0.0	0.0034051826880512137
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.8576244E-4	44.000004	12
CGCATTA	20	7.8576244E-4	44.000004	12
TACGCAA	20	7.8576244E-4	44.000004	34
CGAATTA	40	8.3164196E-9	44.000004	41
CCGAATT	20	7.8576244E-4	44.000004	44
ATACGAC	20	7.8576244E-4	44.000004	37
GACCTAA	20	7.8576244E-4	44.000004	9
AGCGTAA	25	4.4438097E-5	44.0	30
CCGATAG	45	4.802132E-10	44.0	27
TAGACGC	35	1.4466787E-7	44.0	27
CGTTCGA	30	2.528488E-6	44.0	19
CCGTACG	35	1.4466787E-7	44.0	34
TACGCGC	35	1.4466787E-7	44.0	37
TATCTCG	30	2.528488E-6	44.0	13
ATCGCGT	55	1.8189894E-12	44.0	19
GCGTAAT	25	4.4438097E-5	44.0	31
CCGCAAT	25	4.4438097E-5	44.0	19
ACGACGC	25	4.4438097E-5	44.0	21
ACGAAAT	25	4.4438097E-5	44.0	20
CGTAGAT	85	0.0	43.999996	32
>>END_MODULE