FastQCFastQC Report
Fri 17 Jun 2016
SRR1527278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860750
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC61780.3320166599489453No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA59680.32073088808276234No Hit
AGATGGGGGAGTTAACTTTGCATCTTCATACTCCTCAAGCAGACATCACTCT47440.2549509606341529No Hit
AGATGGGGGAACAACAAAACACTATTGTTTTCTTTTCTACAGCATTAGAGAT44940.241515517936316No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT40330.2167405616015048No Hit
AGATGGGGGAATTCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGC36490.19610372161762732No Hit
AGATGGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA22810.12258497917506381No Hit
AGATGGGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGAC22280.1197366653231224No Hit
AGATGGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC21250.1142012629316136No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG20250.10882708585247886No Hit
AGATGGGGGTGTGTGGTTCCTCAGCGATGTAATCTTGTTTAAATGTAAAGTT18850.10130323794169019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT351.0200347E-746.00000422
TCGCATA301.8620285E-646.019
CGCATTA206.312467E-446.012
ACGGGTA301.8620285E-646.015
CGTTACG206.312467E-446.022
TACGCTA301.8620285E-646.034
CGTAAAC206.312467E-446.022
TATACGC253.4179764E-546.019
TATCGTC206.312467E-446.024
TATCGCC253.4179764E-546.030
TAATGCG405.6152203E-946.039
GATGGGG1843600.044.6389122
AGATGGG1893100.044.60891
ATGGGGG1678600.044.5928153
TGGGGGA705900.044.556594
TAGCGTT2400.044.08333214
TGGGGGG743200.043.9946174
ATGGGGT55100.043.7041743
AATACGC800.043.12523
CGCTAGT750.042.9333346
Produced by FastQC (version 0.11.5)