##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1860750
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25268467015988	33.0	31.0	34.0	31.0	34.0
2	32.70519145505845	34.0	31.0	34.0	31.0	34.0
3	32.10951659277173	33.0	31.0	34.0	31.0	34.0
4	36.30119252989386	37.0	37.0	37.0	35.0	37.0
5	36.292158000806126	37.0	37.0	37.0	35.0	37.0
6	36.40682090554884	37.0	37.0	37.0	35.0	37.0
7	36.64573639661427	37.0	37.0	37.0	35.0	37.0
8	36.64016767432487	37.0	37.0	37.0	35.0	37.0
9	38.51289318823055	39.0	39.0	39.0	37.0	39.0
10	36.316081687491604	37.0	35.0	39.0	33.0	39.0
11	35.82315571678087	37.0	35.0	39.0	32.0	39.0
12	35.7569781002284	37.0	35.0	39.0	32.0	39.0
13	35.892734650006716	37.0	35.0	39.0	32.0	39.0
14	36.559753862689774	38.0	35.0	40.0	33.0	40.0
15	36.5719731291146	38.0	35.0	40.0	33.0	40.0
16	31.699871019750102	35.0	33.0	40.0	3.0	40.0
17	33.11141045277442	35.0	33.0	40.0	17.0	40.0
18	35.092529893860004	35.0	33.0	40.0	27.0	40.0
19	36.09751874244257	37.0	35.0	40.0	31.0	40.0
20	36.34863898965471	38.0	35.0	40.0	32.0	40.0
21	36.58876635765149	38.0	35.0	40.0	33.0	40.0
22	36.64233669219401	38.0	35.0	40.0	33.0	40.0
23	36.627132607819426	38.0	35.0	40.0	33.0	40.0
24	36.5847131532984	38.0	35.0	40.0	33.0	40.0
25	36.552688969501546	38.0	35.0	40.0	33.0	40.0
26	36.44672363294371	38.0	35.0	40.0	33.0	40.0
27	36.366498992341796	38.0	35.0	40.0	33.0	40.0
28	36.26566706972995	37.0	35.0	40.0	32.0	40.0
29	36.11794007792557	37.0	35.0	40.0	32.0	40.0
30	36.1034088405213	37.0	35.0	40.0	32.0	40.0
31	36.10233078059922	37.0	35.0	40.0	32.0	40.0
32	36.050786510815534	37.0	35.0	40.0	32.0	40.0
33	35.771817815397014	36.0	35.0	40.0	31.0	40.0
34	35.75811742576918	36.0	35.0	40.0	31.0	40.0
35	35.71909928792154	36.0	35.0	40.0	31.0	40.0
36	35.729691790944514	36.0	35.0	40.0	31.0	40.0
37	35.487661964261726	36.0	34.0	40.0	30.0	40.0
38	35.46775010076582	35.0	34.0	40.0	30.0	40.0
39	35.1346822517802	35.0	34.0	40.0	30.0	40.0
40	35.116968964127366	35.0	34.0	40.0	29.0	40.0
41	35.092679295982805	35.0	34.0	40.0	30.0	40.0
42	35.08129302700524	35.0	34.0	40.0	30.0	40.0
43	35.01124385328497	35.0	34.0	40.0	29.0	40.0
44	35.272841058712885	35.0	34.0	40.0	30.0	40.0
45	35.360719602310894	35.0	34.0	40.0	31.0	40.0
46	35.34578745129652	35.0	35.0	40.0	31.0	40.0
47	35.08188042455999	35.0	34.0	40.0	30.0	40.0
48	35.08958645707376	35.0	34.0	40.0	30.0	40.0
49	35.06325728872766	35.0	34.0	40.0	30.0	40.0
50	34.59421523579202	35.0	34.0	40.0	28.0	40.0
51	34.68255810828967	35.0	34.0	39.0	29.0	40.0
52	34.372527206771466	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	12.0
15	63.0
16	132.0
17	297.0
18	557.0
19	1046.0
20	1721.0
21	2536.0
22	3826.0
23	5419.0
24	7530.0
25	9980.0
26	13387.0
27	17877.0
28	23802.0
29	31986.0
30	42154.0
31	58207.0
32	89644.0
33	158430.0
34	283085.0
35	197180.0
36	129289.0
37	232662.0
38	360824.0
39	189096.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.78876796990461	0.9818084105871289	0.16745935778583906	0.06196426172242375
8	97.49638586591428	1.775198172779793	0.46878946661292487	0.25962649469300014
9	90.57138250705361	4.862555421201129	3.0418917103318557	1.5241703614134086
10	42.98352814725245	41.07327690447401	6.767486228671235	9.175708719602312
11	30.499046083568455	22.72900712078463	26.290044336960904	20.48190245868601
12	28.1279591562542	16.812844283219132	29.508853956737873	25.550342603788796
13	24.460459492140266	18.278973532177886	30.445573021631063	26.814993954050788
14	23.771436248824397	20.300147789869676	31.61961574633884	24.308800214967082
15	24.094693000134352	22.709713825070533	28.073518742442566	25.122074432352548
16	27.90450087330378	29.84371893053876	22.299932822786513	19.951847373370953
17	28.855515249227466	20.979604997984683	24.558914416230017	25.60596533655784
18	26.638291011688835	21.5235792019347	26.09560661023781	25.742523176138654
19	28.05578395808142	21.263146580679834	25.66787585650947	25.013193604729278
20	26.98213086121188	21.577159747413678	27.399650678489856	24.04105871288459
21	27.430014778986965	20.48888888888889	27.66019078328631	24.420905548837833
22	26.652371355636166	21.15012763670563	27.079242241031842	25.11825876662636
23	25.736557839580815	22.31933360204219	27.380464866317343	24.563643692059653
24	26.095875319091768	23.128469703076714	25.372027408303104	25.403627569528414
25	26.816606207174527	22.958376998522102	25.936047292758296	24.288969501545076
26	26.592341797662232	23.030444713153297	25.289695015450757	25.08751847373371
27	25.91143356173586	21.265081284428323	25.50541448340723	27.31807067042859
28	26.353352142953113	21.655945183393793	25.050167943033724	26.940534730619376
29	26.775010076582024	22.23490528012898	25.44216041918581	25.547924224103184
30	26.940427247077793	21.006798334005104	26.507967217519816	25.544807201397283
31	26.9850866586054	22.87690447400242	25.631385194142148	24.506623673250033
32	29.268681983071343	21.77261856778181	24.851108424022573	24.10759102512428
33	28.551927986027138	21.839150880021496	25.62520489050114	23.98371624345022
34	26.630283487840924	22.911137981996507	27.213757893322587	23.244820636839982
35	25.610157194679566	24.25602579604998	27.93185543463657	22.201961574633884
36	25.869246271664654	23.591508800214967	26.18110976756684	24.35813516055354
37	26.952142953110304	24.754319494827353	23.983071342200727	24.310466209861616
38	27.29168346097004	24.796453043127773	25.092946392583638	22.818917103318554
39	27.326938062609162	23.089668144565366	24.432406287787185	25.15098750503829
40	25.551578664516995	23.301034529087733	25.51014375923687	25.637243047158403
41	24.455945183393794	25.341502082493616	25.017922880558913	25.184629853553673
42	24.98551659277173	23.737847642079807	25.41023780733575	25.86639795781271
43	24.932527206771464	23.373532177885263	26.92914147521161	24.764799140131668
44	25.43759236866855	23.164369205965336	26.44869004433696	24.949348381029154
45	26.32336423485154	22.84551927986027	25.621711675399705	25.209404809888486
46	24.97836893725648	23.945559586188363	25.735644229477362	25.34042724707779
47	24.465994894531775	23.437861077522506	26.49361816471853	25.602525863227193
48	26.069756818487168	23.94776299879081	24.612118769313447	25.37036141340857
49	25.23235254601639	24.599596936719063	24.731747951094988	25.436302566169555
50	24.501142012629316	24.938116350933765	25.623431412065028	24.937310224371892
51	24.095069192529895	25.338331318016927	25.529168346097002	25.037431143356176
52	24.834932151014378	25.122934300685205	25.79470643557705	24.247427112723365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	77.0
8	153.0
9	272.5
10	392.0
11	720.0
12	1048.0
13	2096.0
14	3681.5
15	4219.0
16	4415.0
17	4611.0
18	5329.0
19	6047.0
20	5929.5
21	5812.0
22	5896.5
23	5981.0
24	7907.0
25	9833.0
26	10346.0
27	10859.0
28	11965.5
29	13072.0
30	17827.5
31	22583.0
32	22918.0
33	23253.0
34	27171.5
35	31090.0
36	32773.5
37	34457.0
38	39190.0
39	48710.5
40	53498.0
41	57861.5
42	62225.0
43	70271.0
44	78317.0
45	79043.5
46	79770.0
47	83466.5
48	87163.0
49	91891.0
50	96619.0
51	100140.5
52	103662.0
53	107689.5
54	111717.0
55	121327.5
56	130938.0
57	139497.5
58	148057.0
59	147050.0
60	146043.0
61	138159.0
62	130275.0
63	124053.5
64	104912.5
65	91993.0
66	79214.5
67	66436.0
68	55262.5
69	44089.0
70	39641.5
71	35194.0
72	29580.0
73	23966.0
74	20533.5
75	17101.0
76	12714.0
77	8327.0
78	6642.5
79	4958.0
80	3186.0
81	1414.0
82	975.5
83	537.0
84	321.0
85	105.0
86	56.5
87	8.0
88	7.0
89	6.0
90	6.0
91	8.5
92	11.0
93	8.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1860750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.31381761026722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.06384569632655	6.5256187988949135
2	9.821499361126444	1.829513074177754
3	4.012337784122733	1.1211054693630855
4	2.0589286939179283	0.7670594531078915
5	1.208256518679692	0.5626740420699539
6	0.751586046739743	0.4200081214653444
7	0.48817458866733016	0.31827383565703143
8	0.35876826522581146	0.26732017493321464
9	0.27445245794365725	0.2300580122379082
>10	4.816331089025302	12.63980597920138
>50	3.411921082724143	22.721062202283594
>100	2.620894637630051	42.19440309766108
>500	0.08392678239511257	5.34231259287286
>1k	0.027755313862950614	4.313204829323034
>5k	0.001321681612521458	0.7475803167509678
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6178	0.3320166599489453	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5968	0.32073088808276234	No Hit
AGATGGGGGAGTTAACTTTGCATCTTCATACTCCTCAAGCAGACATCACTCT	4744	0.2549509606341529	No Hit
AGATGGGGGAACAACAAAACACTATTGTTTTCTTTTCTACAGCATTAGAGAT	4494	0.241515517936316	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4033	0.2167405616015048	No Hit
AGATGGGGGAATTCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGC	3649	0.19610372161762732	No Hit
AGATGGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA	2281	0.12258497917506381	No Hit
AGATGGGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGAC	2228	0.1197366653231224	No Hit
AGATGGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2125	0.1142012629316136	No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2025	0.10882708585247886	No Hit
AGATGGGGGTGTGTGGTTCCTCAGCGATGTAATCTTGTTTAAATGTAAAGTT	1885	0.10130323794169019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.687088539567379E-4	0.0	0.0	0.0	0.0
8	0.0011285771866182991	0.0	0.0	0.0	0.0
9	0.0023646379148192934	0.0	2.149670831653903E-4	0.0	0.0
10	0.008759908638989655	0.0	2.149670831653903E-4	0.0	0.0
11	0.012790541448340723	0.0	2.149670831653903E-4	0.0	5.374177079134758E-5
12	0.01553137175869945	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
13	0.01950826279725917	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
14	0.028106946123874783	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
15	0.036490662367325004	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
16	0.04922746204487438	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
17	0.05605266693537552	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
18	0.06443638317882575	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
19	0.07233642348515383	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
20	0.07948407900040307	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
21	0.08598683326615612	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
22	0.09098481794975144	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
23	0.09614402794572081	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
24	0.10318419991938735	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
25	0.1087733440816875	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
26	0.1129652022034126	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
27	0.11758699449146849	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
28	0.12296117157060325	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
29	0.1291414752116082	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
30	0.13354830041649873	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
31	0.13902996103721618	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
32	0.1439204621792288	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
33	0.1498857987370684	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
34	0.15574365175332527	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
35	0.16348246674727931	0.0	2.149670831653903E-4	0.0	0.0036006986430202875
36	0.17003896278382372	0.0	2.149670831653903E-4	5.374177079134758E-5	0.0036006986430202875
37	0.17675668413274218	0.0	2.149670831653903E-4	5.374177079134758E-5	0.0036006986430202875
38	0.18132473465000673	0.0	2.149670831653903E-4	5.374177079134758E-5	0.0036006986430202875
39	0.186161494021228	0.0	2.149670831653903E-4	5.374177079134758E-5	0.0036006986430202875
40	0.1941690178691388	0.0	2.149670831653903E-4	5.374177079134758E-5	0.0036006986430202875
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	35	1.0200347E-7	46.000004	22
TCGCATA	30	1.8620285E-6	46.0	19
CGCATTA	20	6.312467E-4	46.0	12
ACGGGTA	30	1.8620285E-6	46.0	15
CGTTACG	20	6.312467E-4	46.0	22
TACGCTA	30	1.8620285E-6	46.0	34
CGTAAAC	20	6.312467E-4	46.0	22
TATACGC	25	3.4179764E-5	46.0	19
TATCGTC	20	6.312467E-4	46.0	24
TATCGCC	25	3.4179764E-5	46.0	30
TAATGCG	40	5.6152203E-9	46.0	39
GATGGGG	184360	0.0	44.638912	2
AGATGGG	189310	0.0	44.6089	1
ATGGGGG	167860	0.0	44.592815	3
TGGGGGA	70590	0.0	44.55659	4
TAGCGTT	240	0.0	44.083332	14
TGGGGGG	74320	0.0	43.994617	4
ATGGGGT	5510	0.0	43.704174	3
AATACGC	80	0.0	43.125	23
CGCTAGT	75	0.0	42.93333	46
>>END_MODULE