FastQCFastQC Report
Fri 17 Jun 2016
SRR1527277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809507
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC59200.32716093388972795No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA58450.3230161585448412No Hit
AGATGGGGGAGTTAACTTTGCATCTTCATACTCCTCAAGCAGACATCACTCT46570.257362917081835No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT40600.2243705053365364No Hit
AGATGGGGGAACAACAAAACACTATTGTTTTCTTTTCTACAGCATTAGAGAT40520.22392839596641514No Hit
AGATGGGGGAATTCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGC36190.19999922630860228No Hit
AGATGGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA22850.1262774888408832No Hit
AGATGGGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGAC22170.12251955919485252No Hit
AGATGGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC20260.11196419798320757No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG19590.10826153200844209No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18730.10350885627963861No Hit
AGATGGGGGTGTGTGGTTCCTCAGCGATGTAATCTTGTTTAAATGTAAAGTT18200.10057988170258528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.41794E-546.039
CCGTACG301.8620012E-646.034
CGCTAGT405.6152203E-946.046
TTCGACG206.312421E-446.035
TAATGCG253.41794E-546.039
CGCTTAT206.312421E-446.011
CCGTTAG351.0199983E-745.99999642
ATGGGGG1632100.044.6880073
AGATGGG1841950.044.6576771
GATGGGG1797150.044.654932
TGGGGGA690850.044.488534
TGGGGGG720650.044.132944
ATCGCGT900.043.44444319
ATGGGGT53400.042.855813
ATGGGGA90150.042.8363843
TGGGGGT95400.042.7693944
TGGGGGC148450.042.5449644
TAGCGTT3050.042.22950714
CTAGACG508.731149E-1041.39999826
CCGATTA508.731149E-1041.39999817
Produced by FastQC (version 0.11.5)