FastQCFastQC Report
Fri 17 Jun 2016
SRR1527270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426384
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160010.6594586841983792No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132010.5440606268422475No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA128130.5280697531800408No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA120130.49509887964971744No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAA90660.3736424242823889No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAA90480.37290057962795664No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC70300.2897315511477161No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC68390.2818597550923514No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA65090.26825926976109304No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA62940.25939834749981866No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56930.23462897876016328No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA52370.215835580847879No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA48400.19947378485845607No Hit
TAAGGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT44190.18212286266312339No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGA43830.18063917335425886No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC43650.17989732869982658No Hit
TAAGGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA43150.17783664910418137No Hit
TAAGGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG42830.17651781416296844No Hit
TAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG39950.16464829969205205No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG37280.15364427065130665No Hit
TAAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC36580.15075931921740335No Hit
TAAGGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC35370.14577247459594195No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAA34520.14226931928334507No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAA34180.14086805715830636No Hit
TAAGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA34070.1404147076472644No Hit
TAAGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA33840.13946679503326762No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA30970.12763849415426412No Hit
TAAGGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG29300.12075582430480913No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA28240.11638718356204128No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA27650.11395558163917995Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA26580.1095457273044992No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA25330.10439402831538619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA207.8582147E-444.026
GACGTAA551.8189894E-1244.042
ATTACGC207.8582147E-444.042
CAATTCG207.8582147E-444.039
ATCGTTA502.7284841E-1144.012
ACGATCT207.8582147E-444.028
CTACGTT351.4469697E-744.025
AACGCGT207.8582147E-444.023
CGTACGT207.8582147E-444.019
ACGTAAC551.8189894E-1244.043
ATGTTCG502.7284841E-1144.024
CGTCAAA207.8582147E-444.012
ATCGTAC2400.042.1666729
TACTATC2400.041.25000443
CACGTTA800.041.2533
CATTCCG1500.041.06666621
ACCCATA700.040.85714327
TTAACCG603.6379788E-1240.33333628
AGGGGGG2335600.039.485273
CATAGAC900.039.1111133
Produced by FastQC (version 0.11.5)