##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527270_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2426384 Sequences flagged as poor quality 0 Sequence length 50 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.165435479297585 33.0 31.0 34.0 30.0 34.0 2 32.167462775883784 33.0 31.0 34.0 30.0 34.0 3 32.747488443708825 34.0 31.0 34.0 31.0 34.0 4 36.28748582252438 37.0 37.0 37.0 35.0 37.0 5 36.30341405152688 37.0 37.0 37.0 35.0 37.0 6 36.336246859524294 37.0 37.0 37.0 35.0 37.0 7 36.54807853991784 37.0 37.0 37.0 35.0 37.0 8 36.54068028803355 37.0 37.0 37.0 35.0 37.0 9 34.620110831591376 35.0 35.0 35.0 33.0 35.0 10 33.49885549855258 35.0 33.0 35.0 27.0 37.0 11 32.80903434905604 35.0 33.0 35.0 24.0 38.0 12 32.96676206239408 35.0 33.0 35.0 25.0 38.0 13 33.14238183238927 35.0 33.0 35.0 27.0 38.0 14 33.67871120152457 35.0 33.0 35.0 30.0 39.0 15 33.759770094098876 35.0 34.0 35.0 30.0 39.0 16 33.64944872699457 35.0 34.0 35.0 29.0 39.0 17 33.76506480425192 35.0 34.0 35.0 30.0 39.0 18 33.73737792534075 35.0 34.0 35.0 30.0 38.0 19 33.661709770588665 35.0 34.0 35.0 29.0 38.0 20 33.46482296289458 35.0 33.0 35.0 29.0 38.0 21 33.62834036162454 35.0 34.0 35.0 29.0 38.0 22 33.46181931631597 35.0 33.0 35.0 29.0 37.0 23 33.423719823408 35.0 33.0 35.0 29.0 36.0 24 33.433744205368974 35.0 33.0 35.0 29.0 36.0 25 33.394778402759 35.0 33.0 35.0 29.0 36.0 26 33.24362384519515 35.0 33.0 35.0 29.0 36.0 27 33.122659892251185 35.0 33.0 35.0 27.0 36.0 28 32.90457610996446 35.0 33.0 35.0 25.0 35.0 29 33.0471318637116 35.0 33.0 35.0 27.0 35.0 30 33.009778336817256 35.0 33.0 35.0 26.0 35.0 31 32.96404237746375 35.0 33.0 35.0 25.0 35.0 32 32.25333912521678 35.0 33.0 35.0 23.0 35.0 33 32.373033287393916 35.0 33.0 35.0 23.0 35.0 34 32.368097959762345 35.0 33.0 35.0 23.0 35.0 35 32.37401293447368 35.0 33.0 35.0 23.0 35.0 36 32.15077044688722 35.0 33.0 35.0 23.0 35.0 37 32.02039001246299 35.0 33.0 35.0 22.0 35.0 38 31.77399001971658 35.0 33.0 35.0 21.0 35.0 39 31.699863253302034 35.0 33.0 35.0 21.0 35.0 40 31.609750558856305 35.0 33.0 35.0 20.0 35.0 41 31.516731069772963 35.0 33.0 35.0 20.0 35.0 42 31.42910479132734 35.0 33.0 35.0 18.0 35.0 43 31.401003715817446 35.0 33.0 35.0 18.0 35.0 44 31.298812966125723 35.0 32.0 35.0 18.0 35.0 45 31.251459373289634 35.0 32.0 35.0 18.0 35.0 46 30.99540550877355 35.0 32.0 35.0 18.0 35.0 47 30.958564678962606 35.0 32.0 35.0 18.0 35.0 48 30.826240611543763 35.0 31.0 35.0 18.0 35.0 49 30.612402241359984 35.0 31.0 35.0 15.0 35.0 50 30.19815124069397 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 7.0 11 6.0 12 8.0 13 32.0 14 105.0 15 422.0 16 1220.0 17 2638.0 18 4522.0 19 7233.0 20 9894.0 21 13352.0 22 18193.0 23 24788.0 24 32620.0 25 42079.0 26 53304.0 27 63465.0 28 70000.0 29 74879.0 30 87775.0 31 113567.0 32 159091.0 33 294235.0 34 824653.0 35 340904.0 36 52572.0 37 45315.0 38 64268.0 39 25236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.79594491226452 0.9137877598929107 0.22852936715705346 0.0617379606855304 8 97.01164366398723 2.1842379442000937 0.5374252385442699 0.2666931532684027 9 88.990571978714 6.8368403352478415 2.7538509980283417 1.418736688009812 10 49.28366655896181 33.91878614432011 6.2964889316777555 10.501058365040324 11 30.4850757340965 32.24802834176288 17.97852277298235 19.288373151158268 12 30.282304861885013 22.732840308871143 25.56648082084287 21.418374008400978 13 23.70791267993854 23.178194383081983 28.43416375973465 24.679729177244823 14 26.08989343813675 24.812849079123502 26.393802464902507 22.703455017837243 15 25.22234732837012 24.38492835429182 25.18521388205659 25.20751043528147 16 29.225093802135195 25.42969290928394 23.55942010827635 21.78579318030452 17 31.367582377727516 24.798300681178247 20.042458242388676 23.791658698705564 18 29.13660822029819 26.377193387361604 21.390513620267857 23.09568477207235 19 30.36971889033228 24.820020244116346 21.247997019433033 23.562263846118338 20 27.869372696160212 24.37005024761126 23.015730403761317 24.74484665246721 21 28.25232939221492 23.297507731669842 26.146644554200822 22.30351832191442 22 27.926618375327234 25.16019723176546 23.693034573257986 23.220149819649322 23 27.178014691821247 28.387551187281158 22.206501526551445 22.227932594346157 24 28.222202256526586 28.171221043330323 22.014940751340266 21.59163594880283 25 30.528143937645485 27.016622265890312 18.868818785484905 23.5864150109793 26 31.482485871980693 26.48026858073578 19.10748669625253 22.929758851031 27 32.91024833661943 23.855127630251435 19.28322145216915 23.95140258095998 28 33.648548622147196 25.520156743532763 18.007454714505204 22.823839919814837 29 33.41915376955997 25.24563300780091 18.500328060191627 22.834885162447492 30 29.565600498519608 27.6885686684383 18.88101800869112 23.864812824350967 31 30.821749566433017 28.822354581962294 18.486274225349327 21.869621626255366 32 30.48169621955964 29.225135015727105 19.661108876418574 20.63205988829468 33 29.64225777947761 29.9296813694782 19.31681052957817 21.111250321466017 34 26.81970372373046 32.500956155332375 20.43308066653918 20.246259454397986 35 27.100656779800726 33.562453428641135 20.722235227400116 18.614654564158023 36 28.74808768933524 31.942800480055915 18.803618883078688 20.50549294753015 37 28.665495651141782 32.29208567151778 18.484419613713246 20.557999063627193 38 29.055128949086377 31.38555150380154 18.605216651609968 20.954102895502114 39 27.915367064735015 31.36123548457293 18.500163205823974 22.223234244868085 40 27.350122651649535 31.019739661982605 20.053833193756635 21.57630449261123 41 25.854934750641284 31.095242962367042 19.95430236928697 23.0955199177047 42 25.829011401328067 30.602328403088713 19.819987273242816 23.748672922340404 43 25.649814703690758 30.031314087135424 21.68259434615461 22.63627686301921 44 26.19766698098899 30.118892969950345 20.951300371252035 22.732139677808625 45 26.700019452815383 30.041411417154084 20.527583432795467 22.730985697235063 46 27.004464256276005 30.730543887529755 20.133128144597062 22.131863711597173 47 26.304739892778716 30.46677689928717 20.893189206654842 22.335294001279273 48 26.90443886870339 30.49533791848281 21.246430902940343 21.353792309873455 49 25.910779167683266 31.097386069146516 20.868873187426228 22.12296157574399 50 24.71871723519443 31.741307229193733 20.573330519818793 22.96664501579305 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 249.5 6 499.0 7 3303.5 8 6108.0 9 12127.5 10 18147.0 11 18360.0 12 18573.0 13 12067.0 14 5561.0 15 4660.5 16 3760.0 17 4526.5 18 5293.0 19 6593.5 20 7894.0 21 9710.5 22 11527.0 23 13034.0 24 14541.0 25 17262.0 26 19983.0 27 22920.0 28 25857.0 29 28622.5 30 31388.0 31 36630.0 32 41872.0 33 50996.0 34 60120.0 35 69892.0 36 79664.0 37 82480.5 38 85297.0 39 77447.5 40 69598.0 41 67208.5 42 64819.0 43 61095.0 44 57371.0 45 59986.0 46 62601.0 47 66287.5 48 69974.0 49 75011.0 50 80048.0 51 84828.0 52 89608.0 53 103892.5 54 118177.0 55 135034.5 56 151892.0 57 174455.0 58 197018.0 59 197543.0 60 198068.0 61 192741.5 62 187415.0 63 179298.0 64 171181.0 65 160497.0 66 149813.0 67 124375.0 68 98937.0 69 85968.0 70 72999.0 71 64243.0 72 55487.0 73 45928.5 74 36370.0 75 36387.0 76 36404.0 77 25328.5 78 14253.0 79 9609.0 80 4965.0 81 3593.0 82 2221.0 83 1494.0 84 767.0 85 486.5 86 206.0 87 134.0 88 62.0 89 45.0 90 28.0 91 18.5 92 9.0 93 9.0 94 9.0 95 4.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2426384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.174916206317413 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2977158870481 10.531638970196846 2 9.468294582513465 2.6842456464771494 3 4.124249832631692 1.7538268737441849 4 2.2115571757261048 1.2539455060559008 5 1.3951389242139856 0.9887988673452532 6 0.8832699736451424 0.7512166718385648 7 0.6401042928031851 0.6351397299652449 8 0.45459829622278075 0.5155114205194062 9 0.35225708423658464 0.4493893186921753 >10 3.1929444475248756 10.974638302601104 >50 1.5351867103795906 15.70404870185531 >100 1.3432482643862569 34.018875745009716 >500 0.06286511207883407 5.958367932651331 >1k 0.03492506226601892 9.056021868998359 >5k 0.002429569548940447 2.3985003087609393 >10k+ 0.0012147847744702234 2.325834135288535 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16001 0.6594586841983792 No Hit TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13201 0.5440606268422475 No Hit TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA 12813 0.5280697531800408 No Hit TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA 12013 0.49509887964971744 No Hit TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAA 9066 0.3736424242823889 No Hit TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAA 9048 0.37290057962795664 No Hit TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 7030 0.2897315511477161 No Hit TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 6839 0.2818597550923514 No Hit TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA 6509 0.26825926976109304 No Hit TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA 6294 0.25939834749981866 No Hit TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5693 0.23462897876016328 No Hit TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA 5237 0.215835580847879 No Hit TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA 4840 0.19947378485845607 No Hit TAAGGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 4419 0.18212286266312339 No Hit TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGA 4383 0.18063917335425886 No Hit TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4365 0.17989732869982658 No Hit TAAGGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 4315 0.17783664910418137 No Hit TAAGGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 4283 0.17651781416296844 No Hit TAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 3995 0.16464829969205205 No Hit TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 3728 0.15364427065130665 No Hit TAAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 3658 0.15075931921740335 No Hit TAAGGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 3537 0.14577247459594195 No Hit TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAA 3452 0.14226931928334507 No Hit TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAA 3418 0.14086805715830636 No Hit TAAGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 3407 0.1404147076472644 No Hit TAAGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA 3384 0.13946679503326762 No Hit TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA 3097 0.12763849415426412 No Hit TAAGGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 2930 0.12075582430480913 No Hit TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 2824 0.11638718356204128 No Hit TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 2765 0.11395558163917995 Illumina Single End Adapter 2 (97% over 34bp) TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA 2658 0.1095457273044992 No Hit TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 2533 0.10439402831538619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.006717815481803375 0.0 0.0 0.0 0.0 8 0.023450533798442457 0.0 0.0 0.0 0.0 9 0.05551470830668188 0.0 0.0 0.0 0.0 10 0.1451954843091613 0.0 0.0 0.0 0.0 11 0.25544184267617986 0.0 0.0 0.0 0.0 12 0.3179628616080554 0.0 0.0 0.0 0.0 13 0.35979465739965316 0.0 0.0 0.0 0.0 14 0.42384057923230617 0.0 0.0 0.0 0.0 15 0.46934038470415235 0.0 0.0 0.0 0.0 16 0.5201979571246761 0.0 0.0 0.0 0.0 17 0.5590623742985447 0.0 0.0 0.0 0.0 18 0.6009765972739681 0.0 0.0 0.0 0.0 19 0.6412834901647885 0.0 0.0 0.0 0.0 20 0.6743780044708505 0.0 0.0 0.0 0.0 21 0.7063185382033511 0.0 0.0 0.0 0.0 22 0.7432459165573133 0.0 0.0 0.0 0.0 23 0.7888281492129853 0.0 0.0 0.0 0.0 24 0.8382020323246444 0.0 0.0 0.0 0.0 25 0.881806012568497 0.0 0.0 0.0 0.0 26 0.9234729539924431 0.0 0.0 0.0 0.0 27 0.9667060119090796 0.0 0.0 0.0 0.0 28 1.0230449920540194 0.0 0.0 0.0 0.0 29 1.0553152345218235 0.0 0.0 0.0 0.0 30 1.0974767390487243 0.0 0.0 0.0 0.0 31 1.142729262969093 0.0 0.0 0.0 0.0 32 1.1885999907681555 0.0 0.0 0.0 0.0 33 1.2337700875046984 0.0 0.0 0.0 0.0 34 1.2855755725392188 0.0 0.0 0.0 0.0 35 1.354731979769072 0.0 0.0 0.0 0.0 36 1.3995723677703118 0.0 0.0 0.0 0.0 37 1.4392610567824384 0.0 0.0 0.0 0.0 38 1.4817934836365554 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAA 20 7.8582147E-4 44.0 26 GACGTAA 55 1.8189894E-12 44.0 42 ATTACGC 20 7.8582147E-4 44.0 42 CAATTCG 20 7.8582147E-4 44.0 39 ATCGTTA 50 2.7284841E-11 44.0 12 ACGATCT 20 7.8582147E-4 44.0 28 CTACGTT 35 1.4469697E-7 44.0 25 AACGCGT 20 7.8582147E-4 44.0 23 CGTACGT 20 7.8582147E-4 44.0 19 ACGTAAC 55 1.8189894E-12 44.0 43 ATGTTCG 50 2.7284841E-11 44.0 24 CGTCAAA 20 7.8582147E-4 44.0 12 ATCGTAC 240 0.0 42.16667 29 TACTATC 240 0.0 41.250004 43 CACGTTA 80 0.0 41.25 33 CATTCCG 150 0.0 41.066666 21 ACCCATA 70 0.0 40.857143 27 TTAACCG 60 3.6379788E-12 40.333336 28 AGGGGGG 233560 0.0 39.48527 3 CATAGAC 90 0.0 39.11111 33 >>END_MODULE