FastQCFastQC Report
Fri 17 Jun 2016
SRR1527269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591131
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153900.5939491287781281No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128250.49495760731510685No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA124890.4819902969012373No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA115830.4470248706066965No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA84620.32657553786358157No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA83230.3212110850435582No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC66520.25672187164601096No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT63490.2450281363620751No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA58510.22580872985580427No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56600.21843743137649158No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA56040.21627621297418No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA47560.18354919145346182No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA44510.17177826979801483No Hit
TAAGGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG42480.16394385308963538No Hit
TAAGGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC42400.16363510760359085No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC42230.16297902344574627No Hit
TAAGGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG41050.15842502752658974No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG38900.1501274925891435No Hit
TAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36890.14237026225227517No Hit
TAAGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG34250.1321816612128063No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT33560.12951873139567238No Hit
TAAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC33330.1286310881232944No Hit
TAAGGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG33070.12762766529364977No Hit
TAAGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA31580.12187728061607073No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA31080.11994762132829255No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA30290.116898759653603No Hit
TAAGGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG26780.10335255145340008No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA26190.10107555349382182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT301.8623141E-646.017
CGAAACT253.4183504E-546.026
TTCGTAA301.8623141E-646.021
CGCCTAT206.312927E-446.020
TACGCAA206.312927E-446.015
TCGTCTA253.4183504E-546.014
ACGTATA253.4183504E-546.017
CACGTTA253.4183504E-546.033
CGATCTA301.8623141E-646.043
TATCGTC253.4183504E-546.038
TATCGCG206.312927E-446.013
CGTCAAA206.312927E-446.012
GCGTATA301.8623141E-646.029
ACGACCA253.4183504E-546.045
ATCGTAC2700.044.29629529
TTTATCG601.8189894E-1242.16666813
TCGTACT2850.041.96491230
ATTACGT554.7293724E-1141.8181844
TACTATC2050.041.51219643
AGGGGGG2501800.041.2102473
Produced by FastQC (version 0.11.5)