##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2591131
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.050781299749026	33.0	31.0	34.0	30.0	34.0
2	31.956123793046356	31.0	31.0	34.0	30.0	34.0
3	32.60903018797583	34.0	31.0	34.0	31.0	34.0
4	36.20563915911623	37.0	35.0	37.0	35.0	37.0
5	36.244340791723765	37.0	37.0	37.0	35.0	37.0
6	36.32592176929688	37.0	37.0	37.0	35.0	37.0
7	36.533963740158256	37.0	37.0	37.0	35.0	37.0
8	36.49428415622367	37.0	37.0	37.0	35.0	37.0
9	34.570947204135955	35.0	35.0	35.0	33.0	35.0
10	33.427048265795904	35.0	33.0	35.0	27.0	37.0
11	32.73977695454224	35.0	33.0	35.0	24.0	37.0
12	32.91318964575701	35.0	33.0	35.0	25.0	37.0
13	33.1347021821745	35.0	33.0	35.0	27.0	37.0
14	33.663850264614176	35.0	33.0	35.0	30.0	38.0
15	33.65836076987231	35.0	34.0	35.0	29.0	38.0
16	29.14805465258221	35.0	30.0	35.0	3.0	36.0
17	30.160933970532557	35.0	30.0	35.0	10.0	36.0
18	31.948187490327584	35.0	31.0	35.0	23.0	36.0
19	32.97929475584214	35.0	32.0	35.0	27.0	37.0
20	33.419221567724676	35.0	33.0	35.0	29.0	38.0
21	33.68846770001208	35.0	33.0	35.0	30.0	38.0
22	33.74174752260692	35.0	34.0	35.0	30.0	37.0
23	33.631576713026085	35.0	34.0	35.0	30.0	36.0
24	33.579559273537306	35.0	34.0	35.0	29.0	36.0
25	33.53982527321081	35.0	33.0	35.0	29.0	36.0
26	33.3890200842798	35.0	33.0	35.0	29.0	36.0
27	33.26531155699963	35.0	33.0	35.0	29.0	36.0
28	33.109577246383914	35.0	33.0	35.0	27.0	35.0
29	32.90432672064824	35.0	33.0	35.0	25.0	35.0
30	32.70242453970872	35.0	33.0	35.0	24.0	35.0
31	32.60106185291288	35.0	33.0	35.0	23.0	35.0
32	32.52167026676768	35.0	33.0	35.0	23.0	35.0
33	32.206650686514884	35.0	33.0	35.0	23.0	35.0
34	32.12463399187459	35.0	33.0	35.0	23.0	35.0
35	32.08211819471883	35.0	33.0	35.0	23.0	35.0
36	31.974440890869662	35.0	33.0	35.0	23.0	35.0
37	31.606823429614327	35.0	32.0	35.0	21.0	35.0
38	31.518359357361707	35.0	32.0	35.0	21.0	35.0
39	31.134559387387206	35.0	31.0	35.0	18.0	35.0
40	31.10324063121471	35.0	31.0	35.0	18.0	35.0
41	31.084870274795062	34.0	31.0	35.0	18.0	35.0
42	31.045747204599074	34.0	31.0	35.0	18.0	35.0
43	31.028107417185776	34.0	31.0	35.0	18.0	35.0
44	31.26852096632706	35.0	32.0	35.0	18.0	35.0
45	31.40312242028674	35.0	32.0	35.0	20.0	35.0
46	31.357100818136946	35.0	32.0	35.0	20.0	35.0
47	31.13360845129019	35.0	32.0	35.0	18.0	35.0
48	31.060561198951348	35.0	32.0	35.0	18.0	35.0
49	31.046806587548062	35.0	32.0	35.0	18.0	35.0
50	30.595911206341942	35.0	31.0	35.0	15.0	35.0
51	30.670523412363174	35.0	31.0	35.0	17.0	35.0
52	30.289648805868943	34.0	30.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	11.0
13	19.0
14	115.0
15	480.0
16	1341.0
17	2912.0
18	5018.0
19	7785.0
20	11353.0
21	16185.0
22	22233.0
23	30759.0
24	40477.0
25	51593.0
26	63194.0
27	70707.0
28	74585.0
29	85106.0
30	108505.0
31	149971.0
32	236209.0
33	414257.0
34	760171.0
35	258736.0
36	55920.0
37	50159.0
38	53780.0
39	19548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.80785649201063	0.9052031718967509	0.22677355949969336	0.06016677659292409
8	96.98209006028642	2.189931732513717	0.5632289529166993	0.26474925428316826
9	89.11849690347574	6.719228012786695	2.7317414673360783	1.4305336164014864
10	49.43717627553374	33.723458983741075	6.266144012016374	10.573220728708815
11	30.56159646115924	32.024085235366336	17.941200194046537	19.473118109427894
12	30.30271337111092	22.578518801249338	25.618426856843595	21.50034097079615
13	23.7539900529923	22.976916257804024	28.4283967117062	24.84069697749747
14	26.114773818845904	24.62843445584187	26.524594858384237	22.732196866927996
15	25.366722099345807	24.23945373661154	25.19359306804635	25.200231095996305
16	27.799829495305335	32.109376175886126	21.06389063308648	19.026903695722062
17	31.440594859927963	24.568151899691678	20.076754127830664	23.914499112549695
18	29.290066770070677	26.19508623840323	21.300119523096285	23.21472746842981
19	30.380478640408377	24.70454021815184	21.230883347850803	23.684097793588975
20	27.936372186508518	24.18561624248253	23.075676220152513	24.802335350856442
21	28.42175868375624	23.036697102539392	26.08822170704607	22.45332250665829
22	27.876629934958903	24.93015598207887	23.716245917323363	23.476968165638866
23	27.358825161676503	28.165808675825343	22.075881149968872	22.399485012529276
24	28.40057102477644	28.00611007316882	21.90313805052697	21.690180851527767
25	30.56869760733826	26.81628215632479	18.944545837319687	23.670474399017262
26	31.519440738426578	26.228971055496615	19.168965212488292	23.082622993588515
27	33.03360578836037	23.60143118970056	19.27922594419194	24.08573707774713
28	33.72874625018959	25.322146969798133	17.99669719516304	22.952409584849242
29	33.606946155945025	25.04253933899907	18.535187916010422	22.81532658904548
30	29.67561269576876	27.48579674281231	18.871257377569872	23.96733318384906
31	31.00657589292089	28.624257129415685	18.474249275702388	21.894917701961035
32	30.633997277636677	28.932423717673867	19.68171427843671	20.751864726252744
33	29.989954193747824	29.584224031899588	19.404113493296943	21.02170828105565
34	26.870814327797397	32.29755654963026	20.498346089024444	20.3332830335479
35	27.18585050312007	33.45338386982364	20.696946622922578	18.663819004133718
36	28.822085799598707	31.74077265873474	18.903173942189724	20.533967599476828
37	28.887964367683455	32.11535811967824	18.407984775760085	20.58869273687822
38	28.996488406028103	31.39189025950444	18.589372748811233	21.022248585656225
39	28.180667052341235	31.12382199124629	18.542289062189447	22.153221894223023
40	27.481358526450418	30.90959121711716	19.99771528340327	21.61133497302915
41	25.899848367373167	31.083762264432018	19.83157161872557	23.184817749469246
42	25.931456186506974	30.555884669667417	19.748403303422325	23.764255840403283
43	25.733859075438488	29.919521629743922	21.69322971320246	22.653389581615134
44	26.289446577575582	29.904508880485007	21.014877287176912	22.791167254762495
45	26.828670568952322	29.893741381659204	20.46044758061248	22.81714046877599
46	26.763911203254487	30.755141287723394	20.13441234734948	22.346535161672644
47	26.472687023543003	30.3782016424488	20.93024243081496	22.21886890319324
48	26.976019352167064	30.498496602448892	21.2135164142608	21.311967631123245
49	25.87217705318643	31.02849682243005	20.926884823654227	22.172441300729297
50	24.58208403974944	31.769022870707808	20.49309741576169	23.155795673781064
51	24.973418943310854	31.602416087801043	20.585335129717485	22.838829839170614
52	24.8689857826563	30.577535446876286	21.101364616455133	23.452114154012282
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	232.5
6	465.0
7	3313.5
8	6162.0
9	11984.0
10	17806.0
11	18127.5
12	18449.0
13	12161.5
14	4804.5
15	3735.0
16	4251.5
17	4768.0
18	5764.5
19	6761.0
20	7875.5
21	8990.0
22	10203.0
23	11416.0
24	13786.0
25	16156.0
26	19237.0
27	22318.0
28	25894.5
29	29471.0
30	34834.0
31	40197.0
32	50585.0
33	60973.0
34	73809.0
35	86645.0
36	84695.5
37	82746.0
38	78124.5
39	67947.5
40	62392.0
41	62472.0
42	62552.0
43	60347.5
44	58143.0
45	62094.5
46	66046.0
47	68914.5
48	71783.0
49	77023.0
50	82263.0
51	88005.5
52	93748.0
53	109573.5
54	125399.0
55	139906.0
56	154413.0
57	172558.5
58	190704.0
59	203510.5
60	216317.0
61	204256.0
62	192195.0
63	186043.0
64	167839.5
65	155788.0
66	136433.0
67	117078.0
68	99311.0
69	81544.0
70	72485.5
71	63427.0
72	56278.0
73	49129.0
74	45409.5
75	41690.0
76	30449.0
77	19208.0
78	12857.5
79	6507.0
80	4801.5
81	3096.0
82	2000.0
83	904.0
84	607.0
85	310.0
86	203.5
87	97.0
88	62.5
89	20.5
90	13.0
91	11.5
92	10.0
93	15.5
94	21.0
95	10.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2591131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.54002198239111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23241072915262	12.608857492308523
2	9.12604951877259	3.0189011930577685
3	3.8645658422687945	1.9176001195057104
4	2.0900864153705982	1.3828030102130693
5	1.2685276947266393	1.0490737978025266
6	0.8233858678918302	0.8171292212952633
7	0.5677451907350369	0.6573362554608041
8	0.40581692500828065	0.5369776688370661
9	0.30491913859185193	0.4539032329644894
>10	2.8009155452863777	11.29430520148655
>50	1.3512136497945981	16.088302982522727
>100	1.081383424898662	31.72550169251252
>500	0.05232500441214803	5.854421343066518
>1k	0.027748108400381534	8.259385705061343
>5k	0.0018498738933587686	2.0500396856094816
>10k+	0.0010570707962050108	2.285461398295622
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15390	0.5939491287781281	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12825	0.49495760731510685	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	12489	0.4819902969012373	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	11583	0.4470248706066965	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	8462	0.32657553786358157	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	8323	0.3212110850435582	No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6652	0.25672187164601096	No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6349	0.2450281363620751	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	5851	0.22580872985580427	No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5660	0.21843743137649158	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5604	0.21627621297418	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	4756	0.18354919145346182	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	4451	0.17177826979801483	No Hit
TAAGGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4248	0.16394385308963538	No Hit
TAAGGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4240	0.16363510760359085	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4223	0.16297902344574627	No Hit
TAAGGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4105	0.15842502752658974	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG	3890	0.1501274925891435	No Hit
TAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3689	0.14237026225227517	No Hit
TAAGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3425	0.1321816612128063	No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3356	0.12951873139567238	No Hit
TAAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3333	0.1286310881232944	No Hit
TAAGGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3307	0.12762766529364977	No Hit
TAAGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3158	0.12187728061607073	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	3108	0.11994762132829255	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	3029	0.116898759653603	No Hit
TAAGGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2678	0.10335255145340008	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	2619	0.10107555349382182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007371298479312702	0.0	0.0	0.0	0.0
8	0.02385058879693848	0.0	0.0	0.0	0.0
9	0.05557418748801199	0.0	0.0	0.0	0.0
10	0.13171854298373953	0.0	0.0	0.0	0.0
11	0.22912774383078277	0.0	0.0	0.0	0.0
12	0.28250211973072764	0.0	0.0	0.0	0.0
13	0.3193972053130467	0.0	0.0	0.0	0.0
14	0.37863774544783724	0.0	0.0	0.0	0.0
15	0.41912199730542377	0.0	0.0	0.0	0.0
16	0.46991062975974585	0.0	0.0	0.0	0.0
17	0.5091598996731543	0.0	0.0	0.0	0.0
18	0.5511878789609633	0.0	0.0	0.0	0.0
19	0.5861147120697487	0.0	0.0	0.0	0.0
20	0.6176453448320444	0.0	0.0	0.0	0.0
21	0.6474392842353397	0.0	0.0	0.0	0.0
22	0.682366117344125	0.0	0.0	0.0	0.0
23	0.7232748942450228	0.0	0.0	0.0	0.0
24	0.7643766370746983	0.0	0.0	0.0	0.0
25	0.8004612657561505	0.0	0.0	0.0	0.0
26	0.8368932331094028	0.0	0.0	0.0	0.0
27	0.8715113207321437	0.0	0.0	0.0	0.0
28	0.9213737167283321	0.0	0.0	0.0	0.0
29	0.9520552994040055	0.0	0.0	0.0	0.0
30	0.994392024177859	0.0	0.0	0.0	0.0
31	1.034567530549401	0.0	0.0	0.0	0.0
32	1.0766726962087212	0.0	0.0	0.0	0.0
33	1.118623489125019	0.0	0.0	0.0	0.0
34	1.165282650703496	0.0	0.0	0.0	0.0
35	1.236332705679489	0.0	0.0	0.0	0.0
36	1.278978175939387	0.0	0.0	0.0	0.0
37	1.317224023023151	0.0	0.0	0.0	0.0
38	1.3585187317816043	0.0	0.0	0.0	0.0
39	1.4013185747845247	0.0	0.0	0.0	0.0
40	1.449328497864446	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	30	1.8623141E-6	46.0	17
CGAAACT	25	3.4183504E-5	46.0	26
TTCGTAA	30	1.8623141E-6	46.0	21
CGCCTAT	20	6.312927E-4	46.0	20
TACGCAA	20	6.312927E-4	46.0	15
TCGTCTA	25	3.4183504E-5	46.0	14
ACGTATA	25	3.4183504E-5	46.0	17
CACGTTA	25	3.4183504E-5	46.0	33
CGATCTA	30	1.8623141E-6	46.0	43
TATCGTC	25	3.4183504E-5	46.0	38
TATCGCG	20	6.312927E-4	46.0	13
CGTCAAA	20	6.312927E-4	46.0	12
GCGTATA	30	1.8623141E-6	46.0	29
ACGACCA	25	3.4183504E-5	46.0	45
ATCGTAC	270	0.0	44.296295	29
TTTATCG	60	1.8189894E-12	42.166668	13
TCGTACT	285	0.0	41.964912	30
ATTACGT	55	4.7293724E-11	41.81818	44
TACTATC	205	0.0	41.512196	43
AGGGGGG	250180	0.0	41.210247	3
>>END_MODULE