FastQCFastQC Report
Fri 17 Jun 2016
SRR1527268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538186
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140920.5551996583386718No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121460.478530730214413No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA114970.45296128810102965No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA107580.4238460065574391No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA85500.33685474586968805No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA83040.3271627847604549No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC66020.2601070213136468No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT65880.25955544629117017No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA58150.22910062540727905No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA54470.21460208195932057No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52200.20565868695202005No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA47050.1853686057680564No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA45150.17788294474873No Hit
TAAGGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG41470.16338440130077148No Hit
TAAGGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG41180.16224185303992694No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC40800.16074472083606167No Hit
TAAGGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC40010.15763226178065753No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG39210.1544804045093622No Hit
TAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC38610.1521165115558907No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT35520.1399424628455125No Hit
TAAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC33410.13162943929247106No Hit
TAAGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG32410.1276896177033519No Hit
TAAGGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG32230.1269804498173105No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA31750.1250893354545333No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA31350.12351340681888562No Hit
TAAGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA31030.12225266391036747No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA27130.10688735971280278No Hit
TAAGGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG26510.1044446703275489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA301.8622995E-646.042
TATGCGC206.3129026E-446.039
ACTACGT206.3129026E-446.039
ACGTACG253.4183307E-546.018
ACGTAAA206.3129026E-446.026
GCGTAAC351.0202166E-745.99999636
TACTATC2150.042.79069543
ATCGTAC2450.042.244929
TCGTACT2450.042.244930
CGATTTC508.731149E-1041.439
AGGGGGG2452150.041.3093033
TTATCGA451.5897967E-840.8888926
TTAACCG900.040.8888928
AACCGTT850.040.58823443
TCGATTG402.9124385E-740.2524
TCGTAAA402.9124385E-740.2545
TAAGGGG2895050.039.8564451
CGTACTG2600.039.80769331
AAGGGGG2828550.039.676232
GTTCGAT355.375681E-639.428579
Produced by FastQC (version 0.11.5)