##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2538186
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03869732163049	33.0	31.0	34.0	30.0	34.0
2	31.986063669092808	33.0	31.0	34.0	30.0	34.0
3	32.601821537113516	34.0	31.0	34.0	31.0	34.0
4	36.20477813682685	37.0	35.0	37.0	35.0	37.0
5	36.25881869965401	37.0	37.0	37.0	35.0	37.0
6	36.30843405487226	37.0	37.0	37.0	35.0	37.0
7	36.53738890688074	37.0	37.0	37.0	35.0	37.0
8	36.517815873226	37.0	37.0	37.0	35.0	37.0
9	34.50403674120021	35.0	35.0	35.0	33.0	35.0
10	33.332829036169926	35.0	33.0	35.0	26.0	37.0
11	32.57638171513041	35.0	33.0	35.0	22.0	37.0
12	32.80917474133101	35.0	33.0	35.0	25.0	37.0
13	33.003972522108306	35.0	33.0	35.0	27.0	37.0
14	33.5644736043773	35.0	33.0	35.0	29.0	38.0
15	33.7419243506977	35.0	34.0	35.0	30.0	38.0
16	33.819304022636636	35.0	34.0	35.0	30.0	39.0
17	33.85714679696444	35.0	34.0	35.0	30.0	39.0
18	33.83720066220521	35.0	34.0	35.0	30.0	39.0
19	33.75358110083343	35.0	34.0	35.0	30.0	39.0
20	33.75019718807054	35.0	34.0	35.0	29.0	39.0
21	29.715237181199488	35.0	30.0	35.0	8.0	36.0
22	30.06436092547985	35.0	30.0	35.0	10.0	36.0
23	31.832998842480418	35.0	31.0	35.0	22.0	36.0
24	32.788067541149466	35.0	32.0	35.0	25.0	36.0
25	33.20282280337217	35.0	33.0	35.0	28.0	36.0
26	33.27327311710017	35.0	33.0	35.0	29.0	36.0
27	33.23321655702143	35.0	33.0	35.0	28.0	36.0
28	33.09135067327611	35.0	33.0	35.0	27.0	35.0
29	32.999088325284276	35.0	33.0	35.0	27.0	35.0
30	32.77770699231656	35.0	33.0	35.0	24.0	35.0
31	32.5899776454523	35.0	33.0	35.0	23.0	35.0
32	32.534692886967306	35.0	33.0	35.0	23.0	35.0
33	32.23920941964064	35.0	33.0	35.0	23.0	35.0
34	32.15359512659829	35.0	33.0	35.0	23.0	35.0
35	32.09983192721101	35.0	33.0	35.0	23.0	35.0
36	31.931407706133434	35.0	33.0	35.0	22.0	35.0
37	31.758826579297182	35.0	32.0	35.0	21.0	35.0
38	31.61383681101385	35.0	32.0	35.0	21.0	35.0
39	31.426606639544936	35.0	32.0	35.0	21.0	35.0
40	27.194436499137574	34.0	20.0	35.0	8.0	35.0
41	27.808385201084555	34.0	20.0	35.0	10.0	35.0
42	29.32344871494839	34.0	23.0	35.0	17.0	35.0
43	30.261562785390826	34.0	30.0	35.0	18.0	35.0
44	30.96650009100988	34.0	31.0	35.0	18.0	35.0
45	31.28173309599848	35.0	32.0	35.0	20.0	35.0
46	31.321398825775574	35.0	32.0	35.0	20.0	35.0
47	31.321067880762087	35.0	32.0	35.0	20.0	35.0
48	31.171711214229376	35.0	32.0	35.0	18.0	35.0
49	31.103925401842105	35.0	32.0	35.0	18.0	35.0
50	30.997855161126882	35.0	32.0	35.0	18.0	35.0
51	30.860336476522995	35.0	31.0	35.0	17.0	35.0
52	30.423415778039907	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	26.0
14	132.0
15	515.0
16	1512.0
17	2977.0
18	5289.0
19	8246.0
20	11653.0
21	16940.0
22	23403.0
23	31610.0
24	41192.0
25	52663.0
26	64678.0
27	73109.0
28	78817.0
29	92671.0
30	122814.0
31	203129.0
32	247810.0
33	273400.0
34	755990.0
35	262455.0
36	55524.0
37	38985.0
38	52702.0
39	19936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.8075341996213	0.9056073904749298	0.2265791395902428	0.06027927031352313
8	96.9822148573824	2.207245647088117	0.5431438042759672	0.26739569125351725
9	89.03610688893563	6.774483824274502	2.7553930247822658	1.4340162620075914
10	49.300563473283674	33.78759476255877	6.321443739741689	10.590398024415862
11	30.61438365825042	32.19303077079457	17.921815028528247	19.270770542426757
12	30.439888960068334	22.706294968138664	25.485681506398667	21.36813456539434
13	23.892654045054222	23.06462174166905	28.338860903022866	24.70386331025386
14	26.154584415799313	24.766191287793724	26.345311178928572	22.733913117478387
15	25.379503314571906	24.210952231239162	25.167974293452094	25.241570160736842
16	29.009576130354514	25.378439562742837	23.68585280984136	21.926131497061288
17	31.418658837453208	24.72037116271227	20.024812996368272	23.836157003466255
18	29.240095091533874	26.34089857874876	21.35781223283085	23.061194096886517
19	30.403051628210072	24.788096695829225	21.234023038500723	23.574828637459984
20	27.893503470588836	24.32071566071202	23.01596494504343	24.769815923655713
21	33.083391051719616	22.055357645184394	24.669980844587432	20.191270458508555
22	27.888736286466003	25.01794588733844	23.699326999676146	23.393990826519413
23	27.267071837918895	28.37731356173267	22.040386323145743	22.315228277202696
24	28.485579859001664	28.17732821786898	21.854269151275755	21.4828227718536
25	30.654885024186562	26.889597531465387	18.80106501257197	23.65445243177608
26	31.561792555785907	26.343262471702232	19.11908741124567	22.975857561266196
27	33.09706223263385	23.738016047681295	19.165577305997274	23.99934441368757
28	33.62562869703009	25.530004499276256	17.984891572170046	22.859475231523614
29	33.51153146380919	25.173647636540426	18.484894330045158	22.82992656960522
30	29.73828553147799	27.61629762357841	18.813751238088937	23.83166560685466
31	31.01782138897622	28.811718290148946	18.425875802640153	21.744584518234678
32	30.56730279026045	29.137068756978408	19.641665346826436	20.65396310593471
33	29.922275199689857	29.850649243199673	19.321830630221744	20.90524492688873
34	26.80654609236675	32.477249500233626	20.4129248211124	20.303279586287214
35	27.098644464984044	33.6523800856202	20.599317780493628	18.649657668902123
36	28.80316887730056	31.945767567861456	18.897945225448414	20.35311832938957
37	28.70258523213035	32.3704803351685	18.39073259406521	20.53620183863594
38	28.932276830775994	31.597487339383324	18.490725265997053	20.979510563843625
39	27.961268401921686	31.388125220137532	18.468898654393335	22.181707723547447
40	27.938575029568362	34.10841443456075	18.611677788783012	19.34133274708788
41	25.87036568636026	31.237033062194815	19.78960564749786	23.102995603947072
42	25.94526169476941	30.738054657932874	19.682363703842036	23.63431994345568
43	25.65576360440094	30.19152260709026	21.593965138882652	22.55874864962615
44	26.23708427987547	30.132385885037582	20.915055082645637	22.71547475244131
45	26.826048209232894	30.097873047916895	20.339368352043547	22.736710390806664
46	26.64694391979154	30.997885891735276	20.069214785677644	22.28595540279554
47	26.297127160893645	30.646099222042828	20.794693533098048	22.262080083965476
48	26.916624707566744	30.648817698939325	21.1142524621915	21.320305131302433
49	25.822299862973004	31.247749376917216	20.828615396980364	22.101335363129415
50	24.437610167261187	32.10363621893746	20.483605220421197	22.975148393380156
51	24.910428156171378	31.801688292347368	20.56571110233844	22.722172449142814
52	24.750825983596158	30.79124224938598	21.072647946210406	23.38528382080746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	246.0
6	492.0
7	3113.5
8	5735.0
9	11207.5
10	16680.0
11	17413.0
12	18146.0
13	12343.5
14	5530.0
15	4519.0
16	4681.0
17	4843.0
18	5691.0
19	6539.0
20	7658.0
21	8777.0
22	9979.5
23	11182.0
24	13451.5
25	15721.0
26	19022.0
27	22323.0
28	25605.0
29	28887.0
30	33479.0
31	38071.0
32	47638.0
33	57205.0
34	70421.0
35	83637.0
36	82944.0
37	82251.0
38	78388.5
39	68405.5
40	62285.0
41	62143.0
42	62001.0
43	60004.5
44	58008.0
45	61343.0
46	64678.0
47	67774.5
48	70871.0
49	75530.5
50	80190.0
51	85973.5
52	91757.0
53	107318.0
54	122879.0
55	135874.5
56	148870.0
57	166698.5
58	184527.0
59	197674.5
60	210822.0
61	198877.0
62	186932.0
63	182796.5
64	165539.0
65	152417.0
66	133954.0
67	115491.0
68	98169.0
69	80847.0
70	71605.0
71	62363.0
72	54849.5
73	47336.0
74	44043.5
75	40751.0
76	30072.0
77	19393.0
78	12931.5
79	6470.0
80	4810.5
81	3151.0
82	2052.5
83	954.0
84	622.5
85	291.0
86	200.0
87	109.0
88	64.5
89	15.5
90	11.0
91	10.5
92	10.0
93	13.0
94	16.0
95	8.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2538186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.846531405461082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.67815536228169	12.917609524235024
2	8.920062277902435	3.0054421860670417
3	3.7809644400636095	1.9108840854738955
4	2.0387261150770852	1.373818540991191
5	1.2330931673313188	1.038667138465327
6	0.7940309661197374	0.8026000564586855
7	0.5520162953974812	0.6509691899717964
8	0.3931368478799135	0.529839380356235
9	0.3091838205892078	0.4687807449254881
>10	2.827222817526608	11.784502585224375
>50	1.3403037249941125	16.238653096624144
>100	1.053931799000482	31.240467443149207
>500	0.04857694310640675	5.483010296949427
>1k	0.027643232817457987	8.258673770822176
>5k	0.0018786663079825815	2.103574119747252
>10k+	0.0010735236045614752	2.192507840538699
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14092	0.5551996583386718	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12146	0.478530730214413	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	11497	0.45296128810102965	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	10758	0.4238460065574391	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	8550	0.33685474586968805	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	8304	0.3271627847604549	No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6602	0.2601070213136468	No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6588	0.25955544629117017	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	5815	0.22910062540727905	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5447	0.21460208195932057	No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5220	0.20565868695202005	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	4705	0.1853686057680564	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	4515	0.17788294474873	No Hit
TAAGGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4147	0.16338440130077148	No Hit
TAAGGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4118	0.16224185303992694	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4080	0.16074472083606167	No Hit
TAAGGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4001	0.15763226178065753	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG	3921	0.1544804045093622	No Hit
TAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3861	0.1521165115558907	No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3552	0.1399424628455125	No Hit
TAAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3341	0.13162943929247106	No Hit
TAAGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3241	0.1276896177033519	No Hit
TAAGGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3223	0.1269804498173105	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	3175	0.1250893354545333	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	3135	0.12351340681888562	No Hit
TAAGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3103	0.12225266391036747	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	2713	0.10688735971280278	No Hit
TAAGGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2651	0.1044446703275489	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0075250592352175925	0.0	0.0	0.0	0.0
8	0.02513606173858023	0.0	0.0	0.0	0.0
9	0.05807297022361639	0.0	0.0	0.0	0.0
10	0.1399818610614037	0.0	0.0	0.0	0.0
11	0.23902897581185933	0.0	0.0	0.0	0.0
12	0.29887486575057937	0.0	0.0	0.0	0.0
13	0.33571219760884347	0.0	0.0	0.0	0.0
14	0.39161826595844434	0.0	0.0	0.0	0.0
15	0.43515329451821105	0.0	0.0	0.0	0.0
16	0.4849526394046772	0.0	0.0	0.0	0.0
17	0.5185987157757548	0.0	0.0	0.0	0.0
18	0.5555148440658013	0.0	0.0	0.0	0.0
19	0.5897518936752468	0.0	0.0	0.0	0.0
20	0.6224130146490446	0.0	0.0	0.0	0.0
21	0.6493219961027284	0.0	0.0	0.0	0.0
22	0.6828892760420238	0.0	0.0	0.0	0.0
23	0.7256757384998578	0.0	0.0	0.0	0.0
24	0.7703139171045779	0.0	0.0	0.0	0.0
25	0.8097909294275518	0.0	0.0	0.0	0.0
26	0.8494255346140905	0.0	0.0	0.0	0.0
27	0.8870508307901785	0.0	0.0	0.0	0.0
28	0.9407112008339814	0.0	0.0	0.0	0.0
29	0.9686839341167275	0.0	0.0	0.0	0.0
30	1.007688167849007	0.0	0.0	0.0	0.0
31	1.0449982782979657	0.0	0.0	0.0	0.0
32	1.0846722817003955	0.0	0.0	0.0	0.0
33	1.1260404083861466	0.0	0.0	0.0	0.0
34	1.1721363209788407	0.0	0.0	0.0	0.0
35	1.2360008289384623	0.0	0.0	0.0	0.0
36	1.2739019126257887	0.0	0.0	0.0	0.0
37	1.3082571568829078	0.0	0.0	0.0	0.0
38	1.3476159745582081	0.0	0.0	0.0	0.0
39	1.3894962780505447	0.0	0.0	0.0	0.0
40	1.439689605095923	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAA	30	1.8622995E-6	46.0	42
TATGCGC	20	6.3129026E-4	46.0	39
ACTACGT	20	6.3129026E-4	46.0	39
ACGTACG	25	3.4183307E-5	46.0	18
ACGTAAA	20	6.3129026E-4	46.0	26
GCGTAAC	35	1.0202166E-7	45.999996	36
TACTATC	215	0.0	42.790695	43
ATCGTAC	245	0.0	42.2449	29
TCGTACT	245	0.0	42.2449	30
CGATTTC	50	8.731149E-10	41.4	39
AGGGGGG	245215	0.0	41.309303	3
TTATCGA	45	1.5897967E-8	40.88889	26
TTAACCG	90	0.0	40.88889	28
AACCGTT	85	0.0	40.588234	43
TCGATTG	40	2.9124385E-7	40.25	24
TCGTAAA	40	2.9124385E-7	40.25	45
TAAGGGG	289505	0.0	39.856445	1
CGTACTG	260	0.0	39.807693	31
AAGGGGG	282855	0.0	39.67623	2
GTTCGAT	35	5.375681E-6	39.42857	9
>>END_MODULE