FastQCFastQC Report
Fri 17 Jun 2016
SRR1527267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394195
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC87360.6265981444489472No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG79430.569719443836766No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC78220.5610406004898885No Hit
AGTTTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA51370.368456349362894No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC49870.35769745265188874No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT45200.3242014208916256No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG39380.2824569016529252No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC35400.2539099623797245No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG35390.25383823640165115No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT32430.23260734689193405No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA32010.2295948558128526No Hit
AGTTTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT27040.19394704471038843No Hit
AGTTTAGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC26390.18928485613561946No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT25130.18024738289837505No Hit
AGTTTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA22690.16274624424847312No Hit
AGTTTAGGGAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGG20720.14861622656801954No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA19870.14251951843178323No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA19710.1413719027826093No Hit
AGTTTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT19520.14000910919921533No Hit
AGTTTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA19430.139363575396555No Hit
AGTTTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC19360.1388614935500414No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGGGGGCTGGTGAAATGGCTCAGT19300.1384311376816012No Hit
AGTTTAGGGAGACCCTATTGCTTCTACAGTGCTGTGCCTCAGCCAGCATA18840.13513174269022626No Hit
AGTTTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT17980.12896330857591656No Hit
AGTTTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG17920.12853295270747636No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC17610.12630944738720193No Hit
AGTTTAGGGGATTTTGTTTCTGTTTGAACTCTTGGTTGTAGGGGGGGAGG17390.12473147586958783No Hit
AGTTTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT17200.12336868228619383No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC17070.12243624457124003No Hit
AGTTTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC16690.11971065740445204No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT16570.11884994566757161No Hit
AGTTTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC16540.11863476773335149No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG16340.11720024817188414No Hit
AGTTTAGGGGGCCGGCAGCACTGGAGTGCATGACACCGTGGTCAACCAGC16280.11676989230344392No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCC16150.11583745458849012No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG15680.1124663336190418No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTT15550.11153389590408802No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT15380.11031455427684075No Hit
AGTTTAGGGATGTATATACATGTAGTTTTTATAAAAATAATACAATTTAA15370.11024282829876737No Hit
AGTTTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT15050.1079475970004196No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG14410.10335713440372402No Hit
AGTTTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA14220.10199434082033002No Hit
AGTTTAGGGATGTTTTCTCCCCTGGACTTGAGAGCAAGCTTCAAAAAGGT14200.10185088886418327No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA13990.10034464332464253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT254.4433298E-544.035
ACAACGT254.4433298E-544.029
TGATATA502.7284841E-1144.034
CGTATTC207.8570604E-444.043
GTCGAAC207.8570604E-444.026
CTATCGA351.4463876E-744.038
ACGCACG207.8570604E-444.037
CGTGAAT351.4463876E-744.028
GAACTAG207.8570604E-444.044
CGTTGAC207.8570604E-444.028
ATTACGC302.528106E-644.026
CCAATTC207.8570604E-444.012
CGCGAAA207.8570604E-444.030
ATACTCG207.8570604E-444.028
TAGTTCG207.8570604E-444.023
CGTTAGA408.314601E-944.038
TTATACG254.4433298E-544.040
AATGCGG302.528106E-644.011
TACGCAT302.528106E-644.012
ATATATC207.8570604E-444.016
Produced by FastQC (version 0.11.5)