##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1394195
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.616815438299522	31.0	31.0	33.0	30.0	34.0
2	32.41467585237359	33.0	31.0	34.0	31.0	34.0
3	30.951817356969435	31.0	31.0	33.0	30.0	33.0
4	35.5028629424148	35.0	35.0	37.0	35.0	37.0
5	36.31269370496953	37.0	35.0	37.0	35.0	37.0
6	36.14140274495318	37.0	36.0	37.0	35.0	37.0
7	36.42805991988208	37.0	36.0	37.0	35.0	37.0
8	36.62447003467951	37.0	37.0	37.0	35.0	37.0
9	38.74557647961727	39.0	39.0	39.0	39.0	39.0
10	38.28777825196619	39.0	39.0	39.0	37.0	39.0
11	37.98493180652635	39.0	38.0	39.0	35.0	39.0
12	37.476261211666944	39.0	37.0	39.0	35.0	39.0
13	37.30390942443489	39.0	37.0	39.0	35.0	39.0
14	38.03290859599984	40.0	38.0	40.0	34.0	40.0
15	38.05286061132051	40.0	38.0	40.0	34.0	40.0
16	37.9187409221809	40.0	38.0	40.0	34.0	40.0
17	37.930991719235834	40.0	38.0	40.0	34.0	40.0
18	37.88756594307109	40.0	37.0	40.0	34.0	40.0
19	37.855445615570275	40.0	37.0	40.0	34.0	40.0
20	37.60604793447115	40.0	37.0	40.0	33.0	40.0
21	37.78311642202131	40.0	37.0	40.0	33.0	40.0
22	37.65770785291871	40.0	37.0	40.0	33.0	40.0
23	37.70943662830522	40.0	37.0	40.0	34.0	40.0
24	37.745917178013116	40.0	37.0	40.0	34.0	40.0
25	37.70129142623521	40.0	37.0	40.0	34.0	40.0
26	37.54639200398796	40.0	36.0	40.0	33.0	40.0
27	37.40246593912616	39.0	36.0	40.0	33.0	40.0
28	37.21095470863114	39.0	36.0	40.0	33.0	40.0
29	37.524622452382914	40.0	36.0	40.0	33.0	40.0
30	37.57051416767382	40.0	36.0	40.0	34.0	40.0
31	37.529292530815276	40.0	36.0	40.0	33.0	40.0
32	36.82762239141584	40.0	35.0	40.0	32.0	40.0
33	37.01022597269392	40.0	35.0	40.0	32.0	40.0
34	36.91611073056495	40.0	35.0	40.0	32.0	40.0
35	36.85603520311004	39.0	35.0	40.0	32.0	40.0
36	36.7020402454463	39.0	35.0	40.0	31.0	40.0
37	36.62982509620247	39.0	35.0	40.0	31.0	40.0
38	36.39600127672241	39.0	35.0	40.0	31.0	40.0
39	36.35417427260892	39.0	35.0	40.0	31.0	40.0
40	36.188832982473755	39.0	35.0	40.0	30.0	40.0
41	36.04329953844333	39.0	35.0	40.0	30.0	40.0
42	35.927879529047225	38.0	35.0	40.0	30.0	40.0
43	35.83879012620186	38.0	35.0	40.0	30.0	40.0
44	35.71946965811813	38.0	35.0	40.0	30.0	40.0
45	35.5451547308662	38.0	35.0	40.0	29.0	40.0
46	35.241296231875744	37.0	34.0	40.0	28.0	40.0
47	35.118425327877375	37.0	34.0	40.0	27.0	40.0
48	34.93918139141225	37.0	34.0	40.0	27.0	40.0
49	34.61765248046364	36.0	34.0	40.0	26.0	40.0
50	34.225284841790426	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	5.0
14	4.0
15	13.0
16	31.0
17	74.0
18	184.0
19	326.0
20	627.0
21	1095.0
22	1691.0
23	2581.0
24	3613.0
25	5103.0
26	7152.0
27	9597.0
28	13493.0
29	18367.0
30	24506.0
31	31532.0
32	38288.0
33	53356.0
34	104541.0
35	101354.0
36	134933.0
37	229676.0
38	475484.0
39	136564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.55350578649328	0.38825271931114375	0.045761174010809105	0.012480320184766119
8	99.07330036329208	0.7828173246927438	0.13448620888756596	0.009396103127611273
9	98.54532543869401	0.8085669508210831	0.17623072812626642	0.46987688235863706
10	59.19702767546864	30.53833932842967	3.5889527648571393	6.675680231244553
11	34.24936970796768	22.342355265941997	20.922109174111224	22.4861658519791
12	31.48641330660345	14.620479918519289	27.942002374129878	25.951104400747383
13	23.479427196339106	15.891822879869746	31.644425636299083	28.984324287492065
14	24.29954202963	15.853234303666271	33.41777871818505	26.429444948518675
15	24.890922718844923	16.70340232176991	31.62814383927643	26.777531120108737
16	30.96231158482135	17.678875623567723	26.972267150577935	24.386545641032995
17	32.07148210974792	17.873396476102695	22.696753323602508	27.358368090546875
18	29.47435616968932	18.793210418915578	24.586159038011182	27.14627437338392
19	29.80694953001553	20.074236387305934	23.203640810647006	26.915173272031527
20	28.279401374987	21.61483867034382	24.870839444984384	25.234920509684798
21	30.359669917048905	19.330007638816664	25.69992002553445	24.610402418599982
22	30.66651365124678	17.915571351209838	25.843156803746965	25.57475819379642
23	29.157327346605026	19.075380416656206	25.957488012795913	25.80980422394285
24	29.934047963161536	18.140647470404065	23.423194029529583	28.502110536904805
25	29.575202894860475	18.718830579653492	24.104662547204658	27.601303978281372
26	28.230197354028668	18.328426081000146	23.905479506094913	29.53589705887627
27	26.489264414231865	17.761647402264387	25.285845954116894	30.46324222938685
28	29.108697133471285	20.641015066041692	22.45611266716636	27.794175133320664
29	30.18422817468145	20.666334336301592	22.139227296038218	27.010210192978747
30	30.269080006742243	18.567488765918686	23.771997460900376	27.3914337664387
31	31.948472057352088	20.372473004135003	22.47655457091727	25.202500367595636
32	34.119115331786446	18.525242164833468	21.876279860421246	25.47936264295884
33	34.001269549811894	18.01311868138962	23.675239116479403	24.310372652319078
34	30.67382970101026	20.743870118598906	26.01809646426791	22.564203716122925
35	28.876950498316234	24.66534451780418	24.57181384239651	21.88589114148308
36	31.14679080042605	23.41229168086243	22.443775798937736	22.997141719773776
37	29.69491355226493	25.486678692722325	21.052220098336317	23.766187656676433
38	31.77747732562518	23.434239830152883	21.49842740793074	23.289855436291194
39	31.404932595512104	21.13470497312069	20.845075473660426	26.61528695770678
40	29.701942698116117	22.652641847087388	21.685273580811867	25.960141873984625
41	27.675898995477677	21.71073630302791	22.63973117103418	27.97363353046023
42	25.86309662565136	20.393201811798207	23.920183331599958	29.823518230950473
43	27.268208536108652	19.937311495163875	25.23190801860572	27.562571950121757
44	27.956419295722622	19.28030153601182	24.42068720659592	28.34259196166964
45	27.978367445013074	19.986730694056426	24.491982828800847	27.542919032129653
46	28.347110698288258	20.7463088018534	24.04821420246092	26.858366297397424
47	27.152084177607865	20.71159342846589	24.711249143771134	27.42507325015511
48	28.875515978754766	21.609459221988317	24.014000910919922	25.501023888337
49	28.4848963021672	21.721208295826624	23.97139567994434	25.82249972206184
50	26.128482744522824	21.793436355746508	24.48158256198021	27.596498337750457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	83.0
8	163.0
9	871.5
10	1580.0
11	1989.0
12	2398.0
13	2249.0
14	2100.0
15	2656.5
16	3213.0
17	4394.0
18	5575.0
19	4823.0
20	4071.0
21	5548.5
22	7026.0
23	6980.5
24	6935.0
25	8102.5
26	9270.0
27	10099.5
28	10929.0
29	10428.0
30	9927.0
31	10640.0
32	11353.0
33	14125.5
34	16898.0
35	17998.5
36	19099.0
37	22851.0
38	26603.0
39	25365.5
40	24128.0
41	29658.0
42	35188.0
43	38309.5
44	41431.0
45	44696.5
46	47962.0
47	58221.5
48	68481.0
49	72485.0
50	76489.0
51	84049.5
52	91610.0
53	112060.0
54	132510.0
55	138254.5
56	143999.0
57	141500.5
58	139002.0
59	136839.0
60	134676.0
61	119243.0
62	103810.0
63	86801.0
64	69792.0
65	57558.0
66	45324.0
67	41471.5
68	37619.0
69	31220.5
70	24822.0
71	24375.0
72	23928.0
73	17641.5
74	11355.0
75	7146.5
76	2938.0
77	2024.0
78	1110.0
79	902.5
80	695.0
81	423.5
82	152.0
83	84.5
84	17.0
85	15.0
86	13.0
87	6.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1394195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.62334093506701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.39012063610014	5.897513268354533
2	11.085925367499623	1.9119542804931455
3	4.6352696925606	1.1991453265479988
4	2.476916324687623	0.8543717574165802
5	1.4465990691835386	0.6237258484960121
6	0.9240077956158635	0.4780820548953184
7	0.6270574430192666	0.3785131081918543
8	0.39158589679533673	0.2701422954744122
9	0.3224816371202142	0.2502782191967551
>10	3.9286426334603144	8.899142765958116
>50	2.8622358027756567	17.932399753512986
>100	2.708673226173684	42.19951188215876
>500	0.13820631894177537	8.048844772505754
>1k	0.05886565436408951	8.875968830173749
>5k	0.0034125017022660587	2.1804058366240326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8736	0.6265981444489472	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7943	0.569719443836766	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7822	0.5610406004898885	No Hit
AGTTTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	5137	0.368456349362894	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4987	0.35769745265188874	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4520	0.3242014208916256	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	3938	0.2824569016529252	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3540	0.2539099623797245	No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3539	0.25383823640165115	No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3243	0.23260734689193405	No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3201	0.2295948558128526	No Hit
AGTTTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT	2704	0.19394704471038843	No Hit
AGTTTAGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC	2639	0.18928485613561946	No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2513	0.18024738289837505	No Hit
AGTTTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2269	0.16274624424847312	No Hit
AGTTTAGGGAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGG	2072	0.14861622656801954	No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1987	0.14251951843178323	No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1971	0.1413719027826093	No Hit
AGTTTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1952	0.14000910919921533	No Hit
AGTTTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1943	0.139363575396555	No Hit
AGTTTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1936	0.1388614935500414	No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGGGGGCTGGTGAAATGGCTCAGT	1930	0.1384311376816012	No Hit
AGTTTAGGGAGACCCTATTGCTTCTACAGTGCTGTGCCTCAGCCAGCATA	1884	0.13513174269022626	No Hit
AGTTTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT	1798	0.12896330857591656	No Hit
AGTTTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	1792	0.12853295270747636	No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1761	0.12630944738720193	No Hit
AGTTTAGGGGATTTTGTTTCTGTTTGAACTCTTGGTTGTAGGGGGGGAGG	1739	0.12473147586958783	No Hit
AGTTTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1720	0.12336868228619383	No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1707	0.12243624457124003	No Hit
AGTTTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	1669	0.11971065740445204	No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1657	0.11884994566757161	No Hit
AGTTTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1654	0.11863476773335149	No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1634	0.11720024817188414	No Hit
AGTTTAGGGGGCCGGCAGCACTGGAGTGCATGACACCGTGGTCAACCAGC	1628	0.11676989230344392	No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCC	1615	0.11583745458849012	No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1568	0.1124663336190418	No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTT	1555	0.11153389590408802	No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	1538	0.11031455427684075	No Hit
AGTTTAGGGATGTATATACATGTAGTTTTTATAAAAATAATACAATTTAA	1537	0.11024282829876737	No Hit
AGTTTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	1505	0.1079475970004196	No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1441	0.10335713440372402	No Hit
AGTTTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA	1422	0.10199434082033002	No Hit
AGTTTAGGGATGTTTTCTCCCCTGGACTTGAGAGCAAGCTTCAAAAAGGT	1420	0.10185088886418327	No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1399	0.10034464332464253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.007387775741556956	0.0	0.0	0.0	0.0
7	0.007459501719630324	0.0	0.0	0.0	0.0
8	0.007602953675777061	0.0	0.0	0.0	0.0
9	0.008105035522290641	0.0	0.0	0.0	0.0
10	0.014632099526967174	0.0	0.0	0.0	0.0
11	0.025247544281825712	0.0	0.0	0.0	0.0
12	0.029622828944301193	0.0	0.0	0.0	0.0
13	0.03399811360677667	0.0	0.0	0.0	0.0
14	0.05178615616897206	0.0	0.0	0.0	0.0
15	0.05960428777896923	0.0	0.0	0.0	0.0
16	0.07481019513052335	0.0	0.0	0.0	0.0
17	0.08434975021428136	0.0	0.0	0.0	0.0
18	0.09388930529803938	0.0	0.0	0.0	0.0
19	0.1070151592854658	0.0	0.0	0.0	0.0
20	0.11483329089546297	0.0	0.0	0.0	0.0
21	0.12623772140912856	0.0	0.0	0.0	0.0
22	0.13498829073407953	0.0	0.0	0.0	0.0
23	0.14474302375205764	0.0	0.0	0.0	0.0
24	0.15291978525242164	0.0	0.0	0.0	0.0
25	0.1593751232790248	0.0	0.0	0.0	0.0
26	0.16410903783186714	0.0	0.0	0.0	0.0
27	0.17092300574883715	0.0	0.0	0.0	0.0
28	0.17866941138076095	0.0	0.0	0.0	0.0
29	0.1837619558239701	0.0	0.0	0.0	0.0
30	0.19000211591635316	0.0	0.0	0.0	0.0
31	0.1953815642718558	0.0	0.0	0.0	0.0
32	0.20205208023267907	0.0	0.0	0.0	0.0
33	0.20865087021542897	0.0	0.0	0.0	0.0
34	0.21467585237359194	0.0	0.0	0.0	0.0
35	0.2333963326507411	0.0	0.0	0.0	0.0
36	0.2486739659803686	0.0	0.0	0.0	0.0
37	0.25699417943687936	0.0	0.0	0.0	0.0
38	0.26474058506880316	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	25	4.4433298E-5	44.0	35
ACAACGT	25	4.4433298E-5	44.0	29
TGATATA	50	2.7284841E-11	44.0	34
CGTATTC	20	7.8570604E-4	44.0	43
GTCGAAC	20	7.8570604E-4	44.0	26
CTATCGA	35	1.4463876E-7	44.0	38
ACGCACG	20	7.8570604E-4	44.0	37
CGTGAAT	35	1.4463876E-7	44.0	28
GAACTAG	20	7.8570604E-4	44.0	44
CGTTGAC	20	7.8570604E-4	44.0	28
ATTACGC	30	2.528106E-6	44.0	26
CCAATTC	20	7.8570604E-4	44.0	12
CGCGAAA	20	7.8570604E-4	44.0	30
ATACTCG	20	7.8570604E-4	44.0	28
TAGTTCG	20	7.8570604E-4	44.0	23
CGTTAGA	40	8.314601E-9	44.0	38
TTATACG	25	4.4433298E-5	44.0	40
AATGCGG	30	2.528106E-6	44.0	11
TACGCAT	30	2.528106E-6	44.0	12
ATATATC	20	7.8570604E-4	44.0	16
>>END_MODULE