FastQCFastQC Report
Fri 17 Jun 2016
SRR1527266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482555
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC85480.5765722013685833No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA79050.5332011291318028No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT73480.49563085349278774No Hit
AGTTTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG48430.3266657898020647No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44710.30157397196056807No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37610.25368367446738904No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT37480.25280680986540127No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG34170.2304804880763277No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC33120.2233981201371956No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC31240.21071730896998764No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG30910.20849142190340325No Hit
AGTTTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA26670.1798921456539555No Hit
AGTTTAGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT23380.15770072611134156No Hit
AGTTTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG22780.1536536587175518No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA21920.14785286211978643No Hit
AGTTTAGGGAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGGCT20280.1367908779100944No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA19270.1299783144638816No Hit
AGTTTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA18950.12781987852052706No Hit
AGTTTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT18740.12640340493270064No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG18540.12505438246810405No Hit
AGTTTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC17450.11770221003605263No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGGGGGCTGGTGAAATGGCTCAGTGG17410.11743240554313332No Hit
AGTTTAGGGAGACCCTATTGCTTCTACAGTGCTGTGCCTCAGCCAGCATAAC17010.11473436061394011No Hit
AGTTTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG17000.11466690949071029No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT16740.11291318028673472No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16710.11271082691704523No Hit
AGTTTAGGGATGTATATACATGTAGTTTTTATAAAAATAATACAATTTAAAA16630.1121712179312066No Hit
AGTTTAGGGGATTTTGTTTCTGTTTGAACTCTTGGTTGTAGGGGGGGAGGGG16340.11021513535754154No Hit
AGTTTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC16090.1085288572767958No Hit
AGTTTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA16060.10832650390710631No Hit
AGTTTAGGGGGCCGGCAGCACTGGAGTGCATGACACCGTGGTCAACCAGCTG15960.10765199267480802No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA15820.1067076769495904No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA15560.10495394774561484No Hit
AGTTTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC15490.10448178988300602No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC15220.10266060955580061No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT15190.10245825618611115No Hit
AGTTTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT14910.10056962473567592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG253.417639E-546.017
TTAGGTC453.110472E-1046.04
AACGTAC206.3120504E-446.035
TGATACG253.417639E-546.032
ACGTTTC301.861772E-646.023
TAGACTA501.6370905E-1146.038
TAGACGG206.3120504E-446.011
TTATGCG253.417639E-546.032
CGCATAT253.417639E-546.036
ATTACGC501.6370905E-1146.026
CGTCTAA253.417639E-546.011
CGATTGT206.3120504E-446.014
CCGTCTA1000.046.037
TTATACG253.417639E-546.040
GTTACGA206.3120504E-446.019
TCAATTC453.110472E-1046.040
ACGATAG206.3120504E-446.038
ACGATAC253.417639E-546.045
GTCAATT206.3120504E-446.023
CGTACTG206.3120504E-446.037
Produced by FastQC (version 0.11.5)