##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1482555
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59823345508261	31.0	31.0	33.0	30.0	34.0
2	32.38842268920883	33.0	31.0	34.0	31.0	34.0
3	30.898477965404318	31.0	31.0	33.0	30.0	33.0
4	35.48441508072213	35.0	35.0	37.0	35.0	37.0
5	36.21565068412302	37.0	35.0	37.0	35.0	37.0
6	36.09569965363848	37.0	35.0	37.0	35.0	37.0
7	36.381772682969604	37.0	36.0	37.0	35.0	37.0
8	36.58540897302292	37.0	37.0	37.0	35.0	37.0
9	38.713974860966374	39.0	39.0	39.0	39.0	39.0
10	38.20675455548024	39.0	39.0	39.0	37.0	39.0
11	37.916274269757274	39.0	38.0	39.0	35.0	39.0
12	37.43985754322774	39.0	37.0	39.0	35.0	39.0
13	37.31286663901171	39.0	37.0	39.0	35.0	39.0
14	38.087585958025166	40.0	38.0	40.0	35.0	40.0
15	38.001885933405504	40.0	38.0	40.0	34.0	40.0
16	32.9213411981343	40.0	35.0	40.0	3.0	40.0
17	34.59992108218582	40.0	34.0	40.0	17.0	40.0
18	36.659460188660795	40.0	34.0	40.0	31.0	40.0
19	37.534013915166724	40.0	37.0	40.0	33.0	40.0
20	37.76733544455349	40.0	37.0	40.0	33.0	40.0
21	37.98270688102634	40.0	38.0	40.0	34.0	40.0
22	38.04545193938842	40.0	38.0	40.0	34.0	40.0
23	38.00154867778936	40.0	37.0	40.0	34.0	40.0
24	37.99949614010947	40.0	37.0	40.0	34.0	40.0
25	37.93941270307004	40.0	37.0	40.0	34.0	40.0
26	37.85596689498872	40.0	37.0	40.0	34.0	40.0
27	37.717342695549235	40.0	37.0	40.0	34.0	40.0
28	37.592908188903614	40.0	36.0	40.0	34.0	40.0
29	37.43084202609684	40.0	36.0	40.0	33.0	40.0
30	37.384713551942426	40.0	36.0	40.0	33.0	40.0
31	37.310047856571934	40.0	36.0	40.0	33.0	40.0
32	37.250597111068394	39.0	35.0	40.0	33.0	40.0
33	36.88449062598015	39.0	35.0	40.0	32.0	40.0
34	36.719530809986814	39.0	35.0	40.0	31.0	40.0
35	36.575749297665176	39.0	35.0	40.0	31.0	40.0
36	36.57852626040855	39.0	35.0	40.0	31.0	40.0
37	36.27103007982841	38.0	35.0	40.0	31.0	40.0
38	36.25626705248709	38.0	35.0	40.0	31.0	40.0
39	35.880276279800746	38.0	35.0	40.0	30.0	40.0
40	35.77756305836883	38.0	34.0	40.0	30.0	40.0
41	35.70013591401331	38.0	34.0	40.0	30.0	40.0
42	35.636669803143896	38.0	34.0	40.0	30.0	40.0
43	35.54337006046993	38.0	34.0	40.0	30.0	40.0
44	35.900423255798266	38.0	35.0	40.0	30.0	40.0
45	35.9218005402835	38.0	35.0	40.0	30.0	40.0
46	35.84767445389885	38.0	35.0	40.0	31.0	40.0
47	35.51899996964699	37.0	35.0	40.0	30.0	40.0
48	35.425584885552304	37.0	35.0	40.0	29.0	40.0
49	35.3130211020839	37.0	34.0	40.0	30.0	40.0
50	34.82698921793795	36.0	34.0	40.0	28.0	40.0
51	34.89211597546128	36.0	34.0	40.0	28.0	40.0
52	34.533166729058955	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	9.0
16	27.0
17	69.0
18	118.0
19	303.0
20	489.0
21	928.0
22	1553.0
23	2409.0
24	3644.0
25	5214.0
26	7511.0
27	10347.0
28	14100.0
29	18391.0
30	25210.0
31	34553.0
32	49270.0
33	72246.0
34	114814.0
35	124923.0
36	185043.0
37	289833.0
38	410499.0
39	111047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.6106046655942	0.3535787879707667	0.02428240436273865	0.011534142072300859
8	99.09204043020327	0.7657051509050254	0.1324740060233853	0.009780412868325289
9	98.55243144436463	0.8171703579293855	0.16282701147680861	0.46757118622917865
10	59.57323674332486	30.238743250671984	3.563240486862208	6.624779519140943
11	34.17249275743564	22.395728994877086	20.683279878318174	22.748498369369095
12	31.633834832434548	14.54543001777337	28.020680514382267	25.800054635409815
13	23.51137057309847	15.835230396174172	31.581964918670806	29.07143411205655
14	24.53669509731511	15.82848528385119	33.29974267396488	26.335076944868824
15	25.018633372792245	16.831281132909066	31.522000870119488	26.628084624179206
16	29.18886651759968	25.934821979623017	23.81119081585506	21.06512068692224
17	32.165619487978525	17.87097274637366	22.61224710044484	27.35116066520298
18	29.571853995298657	18.719507876604915	24.602257589094503	27.106380539001922
19	29.881994259909412	20.068800145694425	23.06619315978159	26.98301243461457
20	28.30849445720395	21.584291982422236	24.720027250253786	25.38718631012003
21	30.42065893002283	19.283938875792128	25.65226922441326	24.64313296977178
22	30.459106070263836	17.90523791697441	25.955664376701034	25.679991636060716
23	29.16998020309533	19.00610769920846	26.01933823702999	25.804573860666213
24	30.072273878540763	17.976196498612193	23.421525676956335	28.53000394589071
25	29.53610489998685	18.576511495357675	24.213266961428076	27.674116643227404
26	28.21203935098529	18.25079002128083	23.925587920852852	29.61158270688103
27	26.576417063785158	17.579921149636945	25.259164078229812	30.584497708348092
28	29.183605329987756	20.71167680119793	22.216781164948348	27.88793670386596
29	30.391722398157235	20.603822455153434	22.076820084246453	26.927635062442878
30	30.344843867512505	18.441879053390934	23.669341103702727	27.54393597539383
31	31.91868092583412	20.3323991352766	22.526921429559106	25.221998509330177
32	34.181666110194904	18.20364168614318	21.931193109193252	25.68349909446867
33	34.288845945007104	17.924866193834294	23.74380714374846	24.042480717410147
34	30.713261902593832	20.71707289105632	25.953371038511218	22.61629416783863
35	28.892014124265202	24.659523592716628	24.629845098495505	21.818617184522665
36	31.09665408703218	23.439804931351617	22.38628583762491	23.077255143991284
37	29.829517286036605	25.478447679849985	20.996657796843962	23.695377237269444
38	31.794908114707383	23.374782048558064	21.47670744087066	23.3536023958639
39	31.63187874986088	21.233276337134203	20.675792803639663	26.45905210936525
40	29.793835641848027	22.582298801730797	21.741992708533576	25.8818728478876
41	27.549939125361284	21.703275763799656	22.662295833881373	28.084489276957687
42	25.930167852120157	20.307779475297714	23.932063228682914	29.829989443899212
43	27.214841945155495	19.802705464552748	25.26800017537292	27.71445241491884
44	27.86284488602446	19.18856298754515	24.465803966800557	28.48278815962983
45	27.933601114292557	19.961215604142847	24.431403893953345	27.67377938761125
46	28.081319074165883	20.737308228025267	24.070270580180836	27.111102117628015
47	27.324787276020114	20.693599900172337	24.720229603623476	27.261383220184076
48	29.013088890462747	21.603785357035658	24.075936474532142	25.30718927796945
49	28.504979579172442	21.601761823338762	24.073912940835246	25.819345656653546
50	26.117884328068776	21.889373412790754	24.38884223519532	27.60390002394515
51	25.29565513589715	22.285311506149856	24.31781620243431	28.101217155518682
52	26.7020110552391	22.253744380478295	25.1361332294586	25.908111334824003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	3.0
6	5.0
7	99.0
8	193.0
9	840.0
10	1487.0
11	1988.0
12	2489.0
13	2309.5
14	2667.0
15	3204.0
16	4415.0
17	5626.0
18	4840.0
19	4054.0
20	5030.5
21	6007.0
22	6418.0
23	6829.0
24	7165.5
25	7502.0
26	8696.0
27	9890.0
28	10891.5
29	11893.0
30	10417.0
31	8941.0
32	11855.0
33	14769.0
34	16626.5
35	18484.0
36	20246.5
37	22009.0
38	22753.0
39	24956.0
40	26415.0
41	32894.0
42	39373.0
43	40233.5
44	41094.0
45	45214.5
46	49335.0
47	56732.5
48	64130.0
49	73851.5
50	83573.0
51	90891.5
52	98210.0
53	117465.5
54	136721.0
55	146451.5
56	156182.0
57	146518.0
58	136854.0
59	137390.0
60	137926.0
61	125079.5
62	112233.0
63	95086.5
64	65483.5
65	53027.0
66	47521.0
67	42015.0
68	35757.0
69	29499.0
70	28566.5
71	27634.0
72	20858.0
73	14082.0
74	9287.0
75	4492.0
76	3185.5
77	1879.0
78	1334.5
79	790.0
80	446.5
81	103.0
82	62.0
83	21.0
84	13.0
85	5.0
86	7.0
87	9.0
88	5.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1482555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.432433325193601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01672463425764	7.61742111370914
2	10.09170660859584	2.105621126631835
3	3.9263818768401397	1.2288515141814982
4	1.9465258307194473	0.8122800377899089
5	1.1302530521970156	0.5895644803820964
6	0.6704622104863815	0.41967313847766563
7	0.43259009045423963	0.3159077093062327
8	0.34373540940315706	0.28687973920852494
9	0.26230148817572424	0.24627985078430709
>10	3.4045434743595018	9.574899388383898
>50	2.4556817706705476	18.51469107124826
>100	2.1643420103040123	40.44630005066871
>500	0.10607382796387574	7.505456001884027
>1k	0.04649811636772635	8.532176127526766
>5k	0.0021795992047371725	1.8039986498171303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8548	0.5765722013685833	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7905	0.5332011291318028	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7348	0.49563085349278774	No Hit
AGTTTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	4843	0.3266657898020647	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4471	0.30157397196056807	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3761	0.25368367446738904	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3748	0.25280680986540127	No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3417	0.2304804880763277	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3312	0.2233981201371956	No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3124	0.21071730896998764	No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3091	0.20849142190340325	No Hit
AGTTTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA	2667	0.1798921456539555	No Hit
AGTTTAGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	2338	0.15770072611134156	No Hit
AGTTTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2278	0.1536536587175518	No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2192	0.14785286211978643	No Hit
AGTTTAGGGAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGGCT	2028	0.1367908779100944	No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1927	0.1299783144638816	No Hit
AGTTTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1895	0.12781987852052706	No Hit
AGTTTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1874	0.12640340493270064	No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1854	0.12505438246810405	No Hit
AGTTTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1745	0.11770221003605263	No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGGGGGCTGGTGAAATGGCTCAGTGG	1741	0.11743240554313332	No Hit
AGTTTAGGGAGACCCTATTGCTTCTACAGTGCTGTGCCTCAGCCAGCATAAC	1701	0.11473436061394011	No Hit
AGTTTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1700	0.11466690949071029	No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1674	0.11291318028673472	No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1671	0.11271082691704523	No Hit
AGTTTAGGGATGTATATACATGTAGTTTTTATAAAAATAATACAATTTAAAA	1663	0.1121712179312066	No Hit
AGTTTAGGGGATTTTGTTTCTGTTTGAACTCTTGGTTGTAGGGGGGGAGGGG	1634	0.11021513535754154	No Hit
AGTTTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	1609	0.1085288572767958	No Hit
AGTTTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	1606	0.10832650390710631	No Hit
AGTTTAGGGGGCCGGCAGCACTGGAGTGCATGACACCGTGGTCAACCAGCTG	1596	0.10765199267480802	No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	1582	0.1067076769495904	No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1556	0.10495394774561484	No Hit
AGTTTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	1549	0.10448178988300602	No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1522	0.10266060955580061	No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT	1519	0.10245825618611115	No Hit
AGTTTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1491	0.10056962473567592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0068800145694426175	0.0	0.0	0.0	0.0
7	0.006947465692672447	0.0	0.0	0.0	0.0
8	0.007082367939132106	0.0	0.0	0.0	0.0
9	0.007689428048200573	0.0	0.0	0.0	0.0
10	0.013692578015655406	0.0	0.0	0.0	0.0
11	0.02293338189814206	0.0	0.0	0.0	0.0
12	0.028734178495907405	0.0	0.0	0.0	0.0
13	0.032916148136156835	0.0	0.0	0.0	0.0
14	0.049576575573924746	0.0	0.0	0.0	0.0
15	0.057940514854423614	0.0	0.0	0.0	0.0
16	0.07460094229219152	0.0	0.0	0.0	0.0
17	0.08363939280498868	0.0	0.0	0.0	0.0
18	0.09315000118039465	0.0	0.0	0.0	0.0
19	0.10751709042834835	0.0	0.0	0.0	0.0
20	0.11432965387456115	0.0	0.0	0.0	0.0
21	0.12734772065791825	0.0	0.0	0.0	0.0
22	0.134969697582889	0.0	0.0	0.0	0.0
23	0.14306383237046855	0.0	0.0	0.0	0.0
24	0.15223718512972537	0.0	0.0	0.0	0.0
25	0.15857759071332936	0.0	0.0	0.0	0.0
26	0.16464819180401402	0.0	0.0	0.0	0.0
27	0.17071879289469868	0.0	0.0	0.0	0.0
28	0.17982469453072567	0.0	0.0	0.0	0.0
29	0.18616510011432966	0.0	0.0	0.0	0.0
30	0.19122393435656687	0.0	0.0	0.0	0.0
31	0.19601296410588478	0.0	0.0	0.0	0.0
32	0.20215101631979926	0.0	0.0	0.0	0.0
33	0.20869377527309274	0.0	0.0	0.0	0.0
34	0.21537143647284587	0.0	0.0	0.0	0.0
35	0.23317853300552088	0.0	0.0	0.0	0.0
36	0.24977150932005895	0.0	0.0	0.0	0.0
37	0.2579330952308683	0.0	0.0	0.0	0.0
38	0.26528526766291977	0.0	0.0	0.0	0.0
39	0.2732445002040397	0.0	0.0	0.0	0.0
40	0.28046177038963144	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	25	3.417639E-5	46.0	17
TTAGGTC	45	3.110472E-10	46.0	4
AACGTAC	20	6.3120504E-4	46.0	35
TGATACG	25	3.417639E-5	46.0	32
ACGTTTC	30	1.861772E-6	46.0	23
TAGACTA	50	1.6370905E-11	46.0	38
TAGACGG	20	6.3120504E-4	46.0	11
TTATGCG	25	3.417639E-5	46.0	32
CGCATAT	25	3.417639E-5	46.0	36
ATTACGC	50	1.6370905E-11	46.0	26
CGTCTAA	25	3.417639E-5	46.0	11
CGATTGT	20	6.3120504E-4	46.0	14
CCGTCTA	100	0.0	46.0	37
TTATACG	25	3.417639E-5	46.0	40
GTTACGA	20	6.3120504E-4	46.0	19
TCAATTC	45	3.110472E-10	46.0	40
ACGATAG	20	6.3120504E-4	46.0	38
ACGATAC	25	3.417639E-5	46.0	45
GTCAATT	20	6.3120504E-4	46.0	23
CGTACTG	20	6.3120504E-4	46.0	37
>>END_MODULE