FastQCFastQC Report
Fri 17 Jun 2016
SRR1527265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439050
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC78910.5483478683853932No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA77030.535283694103749No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT72030.5005385497376742No Hit
AGTTTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG47020.32674333761856783No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT46860.3256314929988534No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38460.26725965046384764No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT36600.2543344567596678No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC33380.23195858378791565No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33310.2314721517667906No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29850.2074285118654668No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC29560.20541329349223447No Hit
AGTTTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA24280.16872242104165944No Hit
AGTTTAGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT23830.16559535804871267No Hit
AGTTTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG21420.1488481984642646No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA21130.1468329800910323No Hit
AGTTTAGGGAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGGCT20100.13967548035162086No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA19360.13453319898544178No Hit
AGTTTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA17950.12473506827420867No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGGGGGCTGGTGAAATGGCTCAGTGG17910.12445710711928008No Hit
AGTTTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT17190.11945380633056531No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG17190.11945380633056531No Hit
AGTTTAGGGAGACCCTATTGCTTCTACAGTGCTGTGCCTCAGCCAGCATAAC17180.11938431604183317No Hit
AGTTTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC16770.11653521420381503No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16530.11486744727424343No Hit
AGTTTAGGGGATTTTGTTTCTGTTTGAACTCTTGGTTGTAGGGGGGGAGGGG16330.11347764149960042No Hit
AGTTTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG16190.11250477745735034No Hit
AGTTTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC16150.11222681630242175No Hit
AGTTTAGGGGGCCGGCAGCACTGGAGTGCATGACACCGTGGTCAACCAGCTG15810.10986414648552864No Hit
AGTTTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC15810.10986414648552864No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT15750.10944720475313575No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA15580.10826586984468921No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA15300.106320141760189No Hit
AGTTTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA15260.10604218060526042No Hit
AGTTTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT15070.10472186511934958No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC14850.10319307876724229No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT14810.10291511761231367No Hit
AGTTTAGGGATGTATATACATGTAGTTTTTATAAAAATAATACAATTTAAAA14760.10256766616865293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT206.311987E-446.021
CAAACGC206.311987E-446.014
ACGTTTC206.311987E-446.023
GCGAACC206.311987E-446.030
ATTACGC405.6134013E-946.026
GCACGTT301.861732E-646.045
ATTCGTT253.417588E-546.010
TAACGGG206.311987E-446.043
CGACACT206.311987E-446.033
TTATACG453.110472E-1046.040
AATGCGG253.417588E-546.011
CTAACGG206.311987E-446.042
CCACTAG600.046.017
GTTAATC301.861732E-646.015
CGTACTG206.311987E-446.037
CGTGTTA206.311987E-446.025
GTATAGT206.311987E-446.026
CGTAACT206.311987E-446.032
CGCACTA301.861732E-646.028
AATAGCG206.311987E-446.037
Produced by FastQC (version 0.11.5)