##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1439050
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.502901914457453	31.0	31.0	33.0	30.0	34.0
2	32.331053820228625	33.0	31.0	34.0	31.0	34.0
3	30.891291477016086	31.0	31.0	33.0	30.0	33.0
4	35.486383377922934	35.0	35.0	37.0	35.0	37.0
5	36.21258886070672	37.0	35.0	37.0	35.0	37.0
6	36.078159202251484	37.0	35.0	37.0	35.0	37.0
7	36.37570758486501	37.0	36.0	37.0	35.0	37.0
8	36.604032521455125	37.0	37.0	37.0	35.0	37.0
9	38.65906674542233	39.0	39.0	39.0	39.0	39.0
10	38.19515235745804	39.0	39.0	39.0	37.0	39.0
11	37.84454744449463	39.0	38.0	39.0	35.0	39.0
12	37.40843403634342	39.0	37.0	39.0	35.0	39.0
13	37.21965046384768	39.0	37.0	39.0	35.0	39.0
14	38.01536082832424	40.0	38.0	40.0	34.0	40.0
15	38.11753101004135	40.0	38.0	40.0	34.0	40.0
16	38.173167019908966	40.0	38.0	40.0	35.0	40.0
17	38.11497793683333	40.0	38.0	40.0	34.0	40.0
18	38.10909975330947	40.0	38.0	40.0	34.0	40.0
19	38.07378061915847	40.0	38.0	40.0	34.0	40.0
20	38.01917584517564	40.0	38.0	40.0	34.0	40.0
21	33.7597095305931	39.0	34.0	40.0	10.0	40.0
22	34.550592404711445	39.0	34.0	40.0	17.0	40.0
23	36.59383621138946	39.0	34.0	40.0	30.0	40.0
24	37.39204336194017	39.0	36.0	40.0	33.0	40.0
25	37.65945380633057	39.0	37.0	40.0	34.0	40.0
26	37.74340641395365	40.0	37.0	40.0	34.0	40.0
27	37.68334943191689	40.0	37.0	40.0	34.0	40.0
28	37.565031791807094	40.0	36.0	40.0	33.0	40.0
29	37.554231611132344	40.0	36.0	40.0	33.0	40.0
30	37.44448003891456	40.0	36.0	40.0	33.0	40.0
31	37.28084639171676	40.0	36.0	40.0	33.0	40.0
32	37.244972030158785	39.0	35.0	40.0	33.0	40.0
33	36.90020986067197	39.0	35.0	40.0	32.0	40.0
34	36.72565303498836	39.0	35.0	40.0	31.0	40.0
35	36.58058997255134	39.0	35.0	40.0	31.0	40.0
36	36.51836072408881	39.0	35.0	40.0	31.0	40.0
37	36.43244779542059	38.0	35.0	40.0	31.0	40.0
38	36.3486049824537	38.0	35.0	40.0	31.0	40.0
39	36.170824502275806	38.0	35.0	40.0	31.0	40.0
40	31.22659462840068	37.0	30.0	40.0	8.0	40.0
41	32.095445606476495	36.0	30.0	40.0	13.0	40.0
42	33.85964976894479	36.0	31.0	40.0	23.0	40.0
43	34.74396164136062	36.0	33.0	40.0	27.0	40.0
44	35.579678954866054	37.0	34.0	40.0	30.0	40.0
45	35.79051527049095	38.0	35.0	40.0	30.0	40.0
46	35.81246655779855	37.0	35.0	40.0	31.0	40.0
47	35.73115666585595	37.0	35.0	40.0	31.0	40.0
48	35.53861088912824	37.0	35.0	40.0	30.0	40.0
49	35.36982314721518	37.0	34.0	40.0	30.0	40.0
50	35.27957749904451	36.0	34.0	40.0	30.0	40.0
51	35.09049720301588	36.0	34.0	40.0	29.0	40.0
52	34.66833466523053	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	10.0
16	23.0
17	51.0
18	146.0
19	303.0
20	552.0
21	933.0
22	1692.0
23	2587.0
24	3762.0
25	5527.0
26	7726.0
27	10748.0
28	14902.0
29	20085.0
30	28114.0
31	39560.0
32	55090.0
33	68888.0
34	128862.0
35	145035.0
36	184209.0
37	207351.0
38	401799.0
39	111093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.60529516000139	0.36447656440012505	0.020291164309787706	0.009937111288697405
8	99.1014905666933	0.7811403356380945	0.11007261735172509	0.007296480316875717
9	98.4891421423856	0.8258920815815989	0.2022167402105556	0.4827490358222438
10	59.5264236822904	30.215767346513324	3.5697161321705293	6.688092839025746
11	34.29151176123137	22.27351377644974	20.73902922066641	22.695945241652478
12	31.64052673638859	14.566971265765611	27.890552795246865	25.90194920259894
13	23.628713387304124	15.826969181056946	31.618637295437964	28.925680136200967
14	24.525207602237586	15.80111879364859	33.37243320245996	26.30124040165387
15	24.921441228588304	16.618533060004864	31.653173968937843	26.80685174246899
16	30.75257982696918	17.59890205343803	27.0939161252215	24.554601994371286
17	32.16635975122477	17.858448281852613	22.600813036378167	27.374378930544456
18	29.53323373058615	18.710677182863694	24.5870539592092	27.16903512734095
19	29.871165004690592	19.969771724401514	23.06090823807373	27.09815503283416
20	28.20596921580209	21.53524894895938	24.854522080539247	25.404259754699282
21	34.879747055349014	18.769188005976165	24.20631666724575	22.14474827142907
22	30.58893019700497	17.82801153538793	25.896181508634168	25.686876758972932
23	29.23963726069282	19.05451513151037	25.849067092873774	25.856780514923038
24	30.169000382196586	18.04433480421111	23.34289982974879	28.443764983843504
25	29.534693026649528	18.63646155449776	24.128834995309408	27.700010423543308
26	28.285674576977865	18.224314652027378	23.905354226746812	29.584656544247938
27	26.523122893575625	17.593620791494388	25.28633473472082	30.596921580209163
28	29.117612313679164	20.684965776032797	22.31291477016087	27.88450714012717
29	30.27219346096383	20.61971439491331	22.066988638337794	27.04110350578507
30	30.389145616900038	18.49296410826587	23.627393071818215	27.490497203015877
31	32.07498002154199	20.28852367881589	22.482610055244777	25.153886244397345
32	34.16795802786561	18.113964073520723	21.927799590007297	25.790278308606375
33	34.24523122893576	17.95087036586637	23.7397588686981	24.064139536499773
34	30.732497133525587	20.685869149786317	25.940238351690354	22.64139536499774
35	28.916576908377056	24.63521072930058	24.591362357110594	21.856850005211772
36	31.20732427643237	23.427677982002017	22.40339112609013	22.96160661547549
37	29.761509329071263	25.45262499565686	20.987526493172577	23.7983391820993
38	31.83940794274	23.336367742607973	21.43685070011466	23.387373614537367
39	31.52315763871999	21.144018623397383	20.63910218546958	26.693721552413052
40	29.88033772280324	27.15798617143254	20.049894027309684	22.911782078454536
41	27.576873631909944	21.64275042562802	22.575866022723325	28.20450991973872
42	25.88332580521872	20.25593273340051	23.921059032000276	29.939682429380493
43	27.190299155692994	19.795907021993674	25.288349953094052	27.725443869219273
44	27.84322990862027	19.253743789305446	24.451478405892775	28.451547896181506
45	27.998401723359162	19.984295194746533	24.369340884611375	27.647962197282926
46	28.02362669816893	20.682742086793372	24.05177026510545	27.241860949932246
47	27.13171884229179	20.70789757131441	24.777248879469095	27.383134706924707
48	28.93360202911643	21.5694381710156	24.145582154893855	25.351377644974114
49	28.48872520065321	21.616066154754872	23.972412355373336	25.92279628921858
50	26.15364302838678	21.78596991070498	24.466627288836385	27.593759772071852
51	25.426635627671036	22.28143566936521	24.310065668322853	27.98186303464091
52	26.672527014349747	22.345714186442443	25.060421806052602	25.921336993155208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	83.0
8	163.0
9	777.0
10	1391.0
11	1826.5
12	2262.0
13	2108.0
14	2512.5
15	3071.0
16	4099.0
17	5127.0
18	4548.5
19	3970.0
20	4902.5
21	5835.0
22	6093.0
23	6351.0
24	6691.5
25	7032.0
26	8273.5
27	9515.0
28	10577.0
29	11639.0
30	10135.0
31	8631.0
32	11361.5
33	14092.0
34	16007.0
35	17922.0
36	19537.0
37	21152.0
38	22022.0
39	24081.5
40	25271.0
41	31230.0
42	37189.0
43	38184.5
44	39180.0
45	43447.0
46	47714.0
47	55228.5
48	62743.0
49	71972.5
50	81202.0
51	87301.0
52	93400.0
53	112848.5
54	132297.0
55	141947.0
56	151597.0
57	141840.0
58	132083.0
59	133520.5
60	134958.0
61	122273.5
62	109589.0
63	93668.0
64	65017.5
65	52288.0
66	46790.0
67	41292.0
68	34854.0
69	28416.0
70	27910.0
71	27404.0
72	20773.0
73	14142.0
74	9422.5
75	4703.0
76	3326.0
77	1949.0
78	1362.0
79	775.0
80	429.5
81	84.0
82	48.0
83	12.0
84	7.5
85	3.0
86	3.0
87	3.0
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1439050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.177968439787984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27868355321421	7.25472191634249
2	9.2554800457569	1.8840396760160238
3	4.214336091961078	1.2868013921591783
4	2.241488571275565	0.9125519974635263
5	1.4094237633454698	0.7172535290808701
6	0.9371053395387003	0.5722697142349017
7	0.6747982376803827	0.48076526164348404
8	0.4578378667660643	0.37278874867878864
9	0.397164790034343	0.3638097628528108
>10	4.241317255390104	11.204771057525335
>50	2.575407361759328	18.861830427346902
>100	2.1684050800451558	39.21756793514794
>500	0.09928733501779635	6.773340452301707
>1k	0.04699095245117079	8.338911256691697
>5k	0.0022737557637663285	1.7585768725143442
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7891	0.5483478683853932	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7703	0.535283694103749	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7203	0.5005385497376742	No Hit
AGTTTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	4702	0.32674333761856783	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4686	0.3256314929988534	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3846	0.26725965046384764	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3660	0.2543344567596678	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3338	0.23195858378791565	No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3331	0.2314721517667906	No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2985	0.2074285118654668	No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2956	0.20541329349223447	No Hit
AGTTTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA	2428	0.16872242104165944	No Hit
AGTTTAGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	2383	0.16559535804871267	No Hit
AGTTTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2142	0.1488481984642646	No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2113	0.1468329800910323	No Hit
AGTTTAGGGAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGGCT	2010	0.13967548035162086	No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1936	0.13453319898544178	No Hit
AGTTTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1795	0.12473506827420867	No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGGGGGCTGGTGAAATGGCTCAGTGG	1791	0.12445710711928008	No Hit
AGTTTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1719	0.11945380633056531	No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1719	0.11945380633056531	No Hit
AGTTTAGGGAGACCCTATTGCTTCTACAGTGCTGTGCCTCAGCCAGCATAAC	1718	0.11938431604183317	No Hit
AGTTTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1677	0.11653521420381503	No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1653	0.11486744727424343	No Hit
AGTTTAGGGGATTTTGTTTCTGTTTGAACTCTTGGTTGTAGGGGGGGAGGGG	1633	0.11347764149960042	No Hit
AGTTTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1619	0.11250477745735034	No Hit
AGTTTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	1615	0.11222681630242175	No Hit
AGTTTAGGGGGCCGGCAGCACTGGAGTGCATGACACCGTGGTCAACCAGCTG	1581	0.10986414648552864	No Hit
AGTTTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	1581	0.10986414648552864	No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1575	0.10944720475313575	No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1558	0.10826586984468921	No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	1530	0.106320141760189	No Hit
AGTTTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	1526	0.10604218060526042	No Hit
AGTTTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1507	0.10472186511934958	No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1485	0.10319307876724229	No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT	1481	0.10291511761231367	No Hit
AGTTTAGGGATGTATATACATGTAGTTTTTATAAAAATAATACAATTTAAAA	1476	0.10256766616865293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.007365970605607866	0.0	0.0	0.0	0.0
7	0.007435460894340016	0.0	0.0	0.0	0.0
8	0.007643931760536465	0.0	0.0	0.0	0.0
9	0.008130363781661513	0.0	0.0	0.0	0.0
10	0.014523470345019284	0.0	0.0	0.0	0.0
11	0.02425211076752024	0.0	0.0	0.0	0.0
12	0.028629998957645668	0.0	0.0	0.0	0.0
13	0.032938396859038946	0.0	0.0	0.0	0.0
14	0.04746186720405823	0.0	0.0	0.0	0.0
15	0.05621764358430909	0.0	0.0	0.0	0.0
16	0.07081060421806053	0.0	0.0	0.0	0.0
17	0.08012230290816859	0.0	0.0	0.0	0.0
18	0.0897814530419374	0.0	0.0	0.0	0.0
19	0.10173378270386714	0.0	0.0	0.0	0.0
20	0.10889128244327856	0.0	0.0	0.0	0.0
21	0.12160800528126195	0.0	0.0	0.0	0.0
22	0.13043327195024496	0.0	0.0	0.0	0.0
23	0.13911955804176365	0.0	0.0	0.0	0.0
24	0.14745839268962163	0.0	0.0	0.0	0.0
25	0.15405997011917585	0.0	0.0	0.0	0.0
26	0.15906327090789063	0.0	0.0	0.0	0.0
27	0.1655953580487127	0.0	0.0	0.0	0.0
28	0.1749765470275529	0.0	0.0	0.0	0.0
29	0.18109169243598208	0.0	0.0	0.0	0.0
30	0.18658142524582189	0.0	0.0	0.0	0.0
31	0.19026441054862583	0.0	0.0	0.0	0.0
32	0.19631006566832285	0.0	0.0	0.0	0.0
33	0.20138285674576978	0.0	0.0	0.0	0.0
34	0.20860984677391334	0.0	0.0	0.0	0.0
35	0.2252180257808971	0.0	0.0	0.0	0.0
36	0.240019457280845	0.0	0.0	0.0	0.0
37	0.24752440846391716	0.0	0.0	0.0	0.0
38	0.2550293596469893	0.0	0.0	0.0	0.0
39	0.26149195649907925	0.0	0.0	0.0	0.0
40	0.26851047566102637	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	6.311987E-4	46.0	21
CAAACGC	20	6.311987E-4	46.0	14
ACGTTTC	20	6.311987E-4	46.0	23
GCGAACC	20	6.311987E-4	46.0	30
ATTACGC	40	5.6134013E-9	46.0	26
GCACGTT	30	1.861732E-6	46.0	45
ATTCGTT	25	3.417588E-5	46.0	10
TAACGGG	20	6.311987E-4	46.0	43
CGACACT	20	6.311987E-4	46.0	33
TTATACG	45	3.110472E-10	46.0	40
AATGCGG	25	3.417588E-5	46.0	11
CTAACGG	20	6.311987E-4	46.0	42
CCACTAG	60	0.0	46.0	17
GTTAATC	30	1.861732E-6	46.0	15
CGTACTG	20	6.311987E-4	46.0	37
CGTGTTA	20	6.311987E-4	46.0	25
GTATAGT	20	6.311987E-4	46.0	26
CGTAACT	20	6.311987E-4	46.0	32
CGCACTA	30	1.861732E-6	46.0	28
AATAGCG	20	6.311987E-4	46.0	37
>>END_MODULE