FastQCFastQC Report
Fri 17 Jun 2016
SRR1527261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810149
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGA198550.7065461653456809No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC133870.4763804339200519No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110300.3925058778022091No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC100810.35873542648450313No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT93890.33411039770489037No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC90510.3220825657287211No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG73210.26051999377968926No Hit
GGACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT61940.22041535875855692No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT60040.2136541514346748No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC59240.2108073272983034No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC56950.2026582932079402No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54280.19315701765280063No Hit
GGACATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA52610.1872142722681253No Hit
GGACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC49540.1762895846448No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT41460.14753666086744865No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT39550.1407398682418619No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC38340.13643404673560014No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG37550.13362280790093337No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37500.13344488139241015No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG36590.13020661893728766No Hit
GGACATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG36100.1284629391537602No Hit
GGACATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA35690.1270039417838698No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT35080.12483323837988662No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT33120.11785851924577666No Hit
GGACATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA32890.11704005730656987No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA32780.11664861898781881No Hit
GGACATGGGGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAAT30300.1078234641650674No Hit
GGACATGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT28740.10227215709914313No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA28340.10084874503095743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.858426E-444.00000411
TAATTCG207.858426E-444.00000415
ATACGTT207.858426E-444.00000440
CAATCGC207.858426E-444.00000431
GGCGTAA302.5290283E-644.025
CGCCTAT254.444489E-544.022
CGTACGA254.444489E-544.043
CGTAACC302.5290283E-644.031
CCTATCG351.4470788E-744.040
TATCGTC351.4470788E-744.018
ATCGCTA454.802132E-1044.035
TAATCGA2050.042.9268326
CGTATAG1350.042.37037331
ATGGGGC353250.041.8140145
ATGGGCT102650.041.7710655
CATGGGG1680700.041.5823174
ATGGGAG274350.041.5381775
ATGGGGT233400.041.4927145
ATGGGGG630450.041.4595955
TGGGATT65700.041.421616
Produced by FastQC (version 0.11.5)