##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2810149
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4568231079562	34.0	31.0	34.0	31.0	34.0
2	32.88937027894251	34.0	31.0	34.0	31.0	34.0
3	32.455327101872534	34.0	31.0	34.0	31.0	34.0
4	36.337635833544766	37.0	37.0	37.0	35.0	37.0
5	35.98232584820236	37.0	35.0	37.0	35.0	37.0
6	36.20746408820315	37.0	37.0	37.0	35.0	37.0
7	36.53671638051932	37.0	37.0	37.0	35.0	37.0
8	36.653252549953756	37.0	37.0	37.0	35.0	37.0
9	38.698447662383735	39.0	39.0	39.0	39.0	39.0
10	38.18629795074923	39.0	39.0	39.0	37.0	39.0
11	37.91399993381134	39.0	38.0	39.0	35.0	39.0
12	37.38393480203363	39.0	37.0	39.0	35.0	39.0
13	37.22963052848799	39.0	37.0	39.0	34.0	39.0
14	38.04029074614905	40.0	38.0	40.0	34.0	40.0
15	38.05161327744543	40.0	38.0	40.0	34.0	40.0
16	37.814790603629916	40.0	37.0	40.0	34.0	40.0
17	37.920735163864975	40.0	38.0	40.0	34.0	40.0
18	37.92235607435762	40.0	38.0	40.0	34.0	40.0
19	37.721520460303	40.0	37.0	40.0	33.0	40.0
20	37.61144088800985	40.0	37.0	40.0	33.0	40.0
21	37.79688799419532	40.0	37.0	40.0	33.0	40.0
22	37.63897181252667	40.0	37.0	40.0	33.0	40.0
23	37.67310274295064	40.0	37.0	40.0	33.0	40.0
24	37.71697265874514	40.0	37.0	40.0	34.0	40.0
25	37.57951553458553	40.0	37.0	40.0	33.0	40.0
26	37.365164267090464	40.0	36.0	40.0	33.0	40.0
27	37.19143931513952	39.0	35.0	40.0	33.0	40.0
28	36.996248597494294	39.0	35.0	40.0	32.0	40.0
29	37.22041784972968	40.0	35.0	40.0	33.0	40.0
30	37.145032523186494	40.0	35.0	40.0	33.0	40.0
31	37.11916521152437	40.0	35.0	40.0	33.0	40.0
32	36.25051198352828	40.0	35.0	40.0	30.0	40.0
33	36.438121252645324	39.0	35.0	40.0	30.0	40.0
34	36.28141675049971	39.0	35.0	40.0	30.0	40.0
35	36.310899172962	39.0	35.0	40.0	30.0	40.0
36	36.08681603715675	39.0	35.0	40.0	30.0	40.0
37	35.89505787771396	39.0	35.0	40.0	29.0	40.0
38	35.666762153892904	39.0	35.0	40.0	28.0	40.0
39	35.57580968126601	39.0	35.0	40.0	28.0	40.0
40	35.2930317929761	38.0	35.0	40.0	27.0	40.0
41	35.16081709546362	38.0	35.0	40.0	26.0	40.0
42	34.99554044999037	38.0	35.0	40.0	26.0	40.0
43	34.85601154956552	38.0	34.0	40.0	25.0	40.0
44	34.690108602782274	37.0	34.0	40.0	24.0	40.0
45	34.620150034749045	37.0	34.0	40.0	24.0	40.0
46	34.25693868901613	37.0	34.0	40.0	23.0	40.0
47	34.182650813177524	36.0	34.0	40.0	23.0	40.0
48	34.048882817245634	36.0	33.0	40.0	23.0	40.0
49	33.71465819072227	35.0	33.0	40.0	22.0	40.0
50	33.26112458805565	35.0	33.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	15.0
15	26.0
16	137.0
17	317.0
18	750.0
19	1538.0
20	2368.0
21	3813.0
22	5904.0
23	8799.0
24	12832.0
25	18348.0
26	24781.0
27	32562.0
28	42333.0
29	52155.0
30	60970.0
31	67939.0
32	79425.0
33	101141.0
34	180100.0
35	221240.0
36	257508.0
37	424792.0
38	799995.0
39	410356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.50319360290149	0.38410774659991337	0.058502236002432616	0.054196414496170846
8	99.14054379322947	0.6729892258382029	0.10647122270029097	0.0799957582320368
9	96.91051969130461	1.6364612694914045	0.5862678455839886	0.8667511936199823
10	57.9487778050203	28.359385925799664	5.599596320337462	8.09223994884257
11	35.165323973924515	27.68529355560862	18.390056897338898	18.75932557312797
12	31.5581842813317	18.462330645101023	25.42285124383084	24.55663382973643
13	24.11331214109999	18.621147846608846	27.384526585600977	29.881013426690185
14	24.04370729096571	22.14259813269688	29.60166880830874	24.21202576802867
15	28.227577968285665	18.65691107482201	27.709918584388234	25.405592372504092
16	29.74493523297163	20.077191636457712	26.32803456329184	23.849838567278816
17	33.60163464641911	20.831671203199544	21.424451158995485	24.142242991385864
18	31.342003573475996	20.75181778617433	21.905208585025207	26.00097005532447
19	28.504716297961423	22.55567231488437	23.97502765867575	24.964583728478456
20	29.063014096405563	24.497348717096497	22.193840967151566	24.245796219346378
21	30.525534411164674	21.494981226974087	24.89255907782826	23.086925284032983
22	30.460306553140065	21.11727883468101	22.160212857040676	26.26220175513825
23	29.584054083964944	24.866581807583866	21.158344272848165	24.39101983560302
24	29.773937253860915	23.69625240512158	20.811423166529604	25.7183871744879
25	27.652946516359094	20.853200310730855	21.591630906403893	29.90222226650615
26	29.963642497248365	21.371642571265795	21.26673710184051	27.39797782964533
27	27.69703670517115	19.859836613645754	22.329670063758186	30.113456617424912
28	28.36725027747639	25.104860987798155	20.28066839160486	26.2472203431206
29	32.651542676206844	22.34020331306276	19.64643155932301	25.361822451407377
30	28.734882029387055	23.992001847588863	21.97289182886744	25.300224294156642
31	33.699423055503466	22.42436255159424	19.826528771250207	24.04968562165209
32	33.367945970124715	22.3458613760338	21.809555294043125	22.47663735979836
33	34.70100695728234	21.069950383413833	21.115997763819642	23.11304489548419
34	30.2153017509036	22.787047946567956	23.872755501576606	23.124894800951836
35	28.555283011683724	28.63912198249986	22.684135254038132	20.121459751778286
36	31.092408267319634	26.498594914362194	20.29878131017252	22.110215508145654
37	30.18971591897796	26.236437996704087	21.24819715965239	22.32564892466556
38	32.78142902742879	26.14847113089021	19.76162118094094	21.308478660740054
39	30.679013817416802	24.007374697925272	19.924566277446498	25.38904520721143
40	29.173897896517232	23.608000856894066	21.8783416822382	25.3397595643505
41	28.89046096843975	23.898412504105654	20.85177689866267	26.35934962879192
42	27.901581019369438	22.611256556147023	21.572735111198728	27.91442731328481
43	26.624388955888108	22.61090070312998	23.96246604717401	26.80224429380791
44	26.612681391627273	21.171439663875475	23.71657161239493	28.49930733210232
45	27.563734164985558	24.02861912304294	22.47816752777166	25.929479184199845
46	28.173452724392906	23.06084125788348	23.7585266831047	25.00717933461891
47	27.85233096181021	22.143203082825856	23.406018684418513	26.598447270945417
48	29.927345489509634	21.81233094757609	23.55145581248539	24.708867750428894
49	28.93430206013987	22.678512776368798	23.77980669352408	24.60737846996725
50	25.872613872075824	23.175105661657085	24.202595663076938	26.74968480319015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	18.0
6	36.0
7	74.0
8	112.0
9	370.5
10	629.0
11	8348.0
12	16067.0
13	14449.0
14	12831.0
15	12271.5
16	11712.0
17	10419.0
18	9126.0
19	8964.0
20	8802.0
21	9701.5
22	10601.0
23	11112.5
24	11624.0
25	13159.0
26	14694.0
27	17646.5
28	20599.0
29	22202.0
30	23805.0
31	23785.0
32	23765.0
33	26838.5
34	29912.0
35	29243.5
36	28575.0
37	35430.5
38	42286.0
39	41533.0
40	40780.0
41	42852.0
42	44924.0
43	49607.5
44	54291.0
45	56175.5
46	58060.0
47	66506.0
48	74952.0
49	86019.5
50	97087.0
51	97126.5
52	97166.0
53	118534.5
54	139903.0
55	172596.5
56	205290.0
57	232400.5
58	259511.0
59	269886.0
60	280261.0
61	272077.0
62	263893.0
63	265294.5
64	266696.0
65	238679.0
66	210662.0
67	176314.5
68	141967.0
69	123276.0
70	104585.0
71	90029.5
72	75474.0
73	61622.0
74	47770.0
75	48731.0
76	49692.0
77	34927.0
78	20162.0
79	13516.0
80	6870.0
81	5240.0
82	3610.0
83	2194.5
84	779.0
85	583.5
86	388.0
87	286.5
88	185.0
89	98.0
90	11.0
91	6.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2810149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.474611579946494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31010406950215	7.678948650134898
2	9.59535541938666	2.010152419792197
3	4.150637994845196	1.3042896241491415
4	2.2809543463310042	0.955684432376321
5	1.3441649754834317	0.7039803008778623
6	0.8766875845215892	0.5509777154895096
7	0.5828535725168051	0.4273615346068398
8	0.40296318600055586	0.3376706283498845
9	0.33892158236029774	0.3195064738156468
>10	2.918657069774799	7.445442892020636
>50	1.8296925853708148	14.16406369232684
>100	2.202025973454388	42.35996657693267
>500	0.1115579470255967	8.014489272332122
>1k	0.05080505230783544	9.461973147019881
>5k	0.003197520774619014	2.242788736009027
>10k+	0.0014211203442751172	2.02270390376655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGA	19855	0.7065461653456809	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	13387	0.4763804339200519	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11030	0.3925058778022091	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	10081	0.35873542648450313	No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	9389	0.33411039770489037	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	9051	0.3220825657287211	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7321	0.26051999377968926	No Hit
GGACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	6194	0.22041535875855692	No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	6004	0.2136541514346748	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5924	0.2108073272983034	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	5695	0.2026582932079402	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5428	0.19315701765280063	No Hit
GGACATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	5261	0.1872142722681253	No Hit
GGACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	4954	0.1762895846448	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	4146	0.14753666086744865	No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	3955	0.1407398682418619	No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	3834	0.13643404673560014	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3755	0.13362280790093337	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3750	0.13344488139241015	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	3659	0.13020661893728766	No Hit
GGACATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	3610	0.1284629391537602	No Hit
GGACATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	3569	0.1270039417838698	No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	3508	0.12483323837988662	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3312	0.11785851924577666	No Hit
GGACATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	3289	0.11704005730656987	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	3278	0.11664861898781881	No Hit
GGACATGGGGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAAT	3030	0.1078234641650674	No Hit
GGACATGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	2874	0.10227215709914313	No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2834	0.10084874503095743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.2026771534178434E-4	0.0	0.0	0.0	0.0
8	0.0014234120681857084	0.0	0.0	0.0	0.0
9	0.004412577411375696	0.0	0.0	0.0	0.0
10	0.033165501188727006	0.0	0.0	0.0	0.0
11	0.066829196601319	0.0	0.0	0.0	0.0
12	0.09116954296729461	0.0	0.0	0.0	0.0
13	0.11013650877586918	0.0	0.0	0.0	0.0
14	0.14903124353904365	0.0	0.0	0.0	0.0
15	0.17394095473229357	0.0	0.0	0.0	0.0
16	0.20176866066532415	0.0	0.0	0.0	0.0
17	0.21995274983639657	0.0	0.0	0.0	0.0
18	0.23742513297337614	0.0	0.0	0.0	0.0
19	0.2598082877455964	0.0	0.0	0.0	0.0
20	0.2827963926467956	0.0	0.0	0.0	0.0
21	0.30137192013661906	0.0	0.0	0.0	0.0
22	0.32126410378951437	0.0	0.0	0.0	0.0
23	0.34489274412139714	0.0	0.0	0.0	0.0
24	0.3716884763049931	0.0	0.0	0.0	0.0
25	0.3935378515516437	0.0	0.0	0.0	0.0
26	0.41734441839204967	0.0	0.0	0.0	0.0
27	0.44556356264383135	0.0	0.0	0.0	0.0
28	0.5175170426906189	0.0	0.0	0.0	0.0
29	0.5387258825065859	0.0	0.0	0.0	0.0
30	0.5690445595589415	0.0	0.0	0.0	0.0
31	0.5856984807567144	0.0	0.0	0.0	0.0
32	0.6021033048425546	0.0	0.0	0.0	0.0
33	0.6159815725073653	0.0	0.0	0.0	0.0
34	0.6367633887028766	0.0	0.0	0.0	0.0
35	0.7129871049542212	0.0	0.0	0.0	0.0
36	0.7330216298139351	0.0	0.0	0.0	0.0
37	0.7472557504957922	0.0	0.0	0.0	0.0
38	0.7606002386350332	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.858426E-4	44.000004	11
TAATTCG	20	7.858426E-4	44.000004	15
ATACGTT	20	7.858426E-4	44.000004	40
CAATCGC	20	7.858426E-4	44.000004	31
GGCGTAA	30	2.5290283E-6	44.0	25
CGCCTAT	25	4.444489E-5	44.0	22
CGTACGA	25	4.444489E-5	44.0	43
CGTAACC	30	2.5290283E-6	44.0	31
CCTATCG	35	1.4470788E-7	44.0	40
TATCGTC	35	1.4470788E-7	44.0	18
ATCGCTA	45	4.802132E-10	44.0	35
TAATCGA	205	0.0	42.92683	26
CGTATAG	135	0.0	42.370373	31
ATGGGGC	35325	0.0	41.814014	5
ATGGGCT	10265	0.0	41.771065	5
CATGGGG	168070	0.0	41.582317	4
ATGGGAG	27435	0.0	41.538177	5
ATGGGGT	23340	0.0	41.492714	5
ATGGGGG	63045	0.0	41.459595	5
TGGGATT	6570	0.0	41.42161	6
>>END_MODULE