FastQCFastQC Report
Fri 17 Jun 2016
SRR1527260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024091
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG190180.6288831916764409No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC125040.4134796208182889No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110780.36632495516834646No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT94090.3111348170408893No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC88970.29420410959855375No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG86320.2854411457856261No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT64600.21361791030759325No Hit
GGACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT56220.1859071039859581No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC55920.18491507034675875No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54790.18117841030577453No Hit
GGACATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG54060.17876446178372277No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG53670.17747481805276363No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49620.1640823639235724No Hit
GGACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC43130.14262136952889315No Hit
GGACATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT39610.13098150816228746No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT38390.1269472380295434No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC36540.12082969725448077No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG36410.1203998160108277No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36230.11980459582730811No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA35590.11768825739701615No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG34090.1127280892010194No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT32990.10909063252395515No Hit
GGACATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG32820.10852848012840882No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG32330.10690815851771655No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT31520.10422966769187833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG253.418488E-546.011
CGTCTAT206.313097E-446.015
CACGATT206.313097E-446.012
TTATCCG206.313097E-446.014
CGCCTAT453.110472E-1046.022
CCGTAAA253.418488E-546.042
AATTCGC206.313097E-446.038
ACGCTAA650.046.018
TCATATC206.313097E-446.016
ATAACGT501.6370905E-1146.038
CGCACTA301.8624178E-646.037
TACGTAT301.8624178E-646.030
CGTATAG1400.044.35714331
ATGGGCT105650.043.38765
ATGGGGC370300.043.366465
ATGGGAG301650.043.354225
CATGGGG1809700.043.2763984
ATGGGGG688500.043.1938975
CTCATAA2450.043.18367445
ATGGGGT250150.043.140525
Produced by FastQC (version 0.11.5)