##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3024091
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38802469899219	33.0	31.0	34.0	31.0	34.0
2	32.824060850020714	34.0	31.0	34.0	31.0	34.0
3	32.3415512297745	34.0	31.0	34.0	31.0	34.0
4	36.29583765832444	37.0	37.0	37.0	35.0	37.0
5	35.99968254923546	37.0	35.0	37.0	35.0	37.0
6	36.22836812781097	37.0	37.0	37.0	35.0	37.0
7	36.52047474761837	37.0	37.0	37.0	35.0	37.0
8	36.60408135866282	37.0	37.0	37.0	35.0	37.0
9	38.657262297993014	39.0	39.0	39.0	38.0	39.0
10	38.06317931570181	39.0	38.0	39.0	35.0	39.0
11	37.80970810732878	39.0	38.0	39.0	35.0	39.0
12	37.32393337369808	39.0	37.0	39.0	35.0	39.0
13	37.213988600210776	39.0	37.0	39.0	34.0	39.0
14	38.03248645626074	40.0	38.0	40.0	34.0	40.0
15	37.95620105347359	40.0	38.0	40.0	34.0	40.0
16	32.827604724857814	39.0	34.0	40.0	3.0	40.0
17	34.56978708643358	40.0	34.0	40.0	17.0	40.0
18	36.62480494138569	40.0	34.0	40.0	31.0	40.0
19	37.30235366594458	40.0	36.0	40.0	32.0	40.0
20	37.68384549274476	40.0	37.0	40.0	33.0	40.0
21	37.89627461607471	40.0	37.0	40.0	34.0	40.0
22	37.94521428091946	40.0	37.0	40.0	34.0	40.0
23	37.88561951343395	40.0	37.0	40.0	34.0	40.0
24	37.85690245432429	40.0	37.0	40.0	34.0	40.0
25	37.73961530919539	40.0	37.0	40.0	34.0	40.0
26	37.553037921147215	40.0	36.0	40.0	33.0	40.0
27	37.405041713361136	40.0	36.0	40.0	33.0	40.0
28	37.28396533040838	40.0	35.0	40.0	33.0	40.0
29	37.05664677418768	40.0	35.0	40.0	32.0	40.0
30	36.91320267809401	39.0	35.0	40.0	32.0	40.0
31	36.87373296636907	39.0	35.0	40.0	32.0	40.0
32	36.61997968976463	39.0	35.0	40.0	31.0	40.0
33	36.2985826154041	39.0	35.0	40.0	30.0	40.0
34	36.05346796772981	39.0	35.0	40.0	30.0	40.0
35	36.0033742370848	38.0	35.0	40.0	30.0	40.0
36	35.94016780579685	38.0	35.0	40.0	30.0	40.0
37	35.45876397238046	38.0	35.0	40.0	28.0	40.0
38	35.44075161759352	38.0	35.0	40.0	28.0	40.0
39	35.00568402207473	38.0	34.0	40.0	26.0	40.0
40	34.81311508152367	38.0	34.0	40.0	25.0	40.0
41	34.76495284037418	37.0	34.0	40.0	26.0	40.0
42	34.64532912534709	37.0	34.0	40.0	25.0	40.0
43	34.493263264895134	37.0	34.0	40.0	25.0	40.0
44	34.721557320861045	37.0	34.0	40.0	26.0	40.0
45	34.83969662288602	37.0	34.0	40.0	26.0	40.0
46	34.71511604644172	37.0	34.0	40.0	26.0	40.0
47	34.41394587662871	36.0	34.0	40.0	24.0	40.0
48	34.35287661647748	36.0	34.0	40.0	24.0	40.0
49	34.24146528659356	36.0	34.0	40.0	25.0	40.0
50	33.695157652332554	35.0	33.0	40.0	23.0	40.0
51	33.74539754260041	35.0	33.0	39.0	23.0	40.0
52	33.35539274446437	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	4.0
15	39.0
16	130.0
17	323.0
18	780.0
19	1361.0
20	2499.0
21	4173.0
22	6951.0
23	10645.0
24	15929.0
25	22485.0
26	30546.0
27	39903.0
28	50087.0
29	57893.0
30	64939.0
31	75804.0
32	95215.0
33	137277.0
34	207776.0
35	266787.0
36	353901.0
37	555967.0
38	704869.0
39	317805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.52382385318431	0.3711859200004233	0.05181722375417935	0.05317300306108513
8	99.12872992247918	0.6737892477441981	0.11911017227986857	0.07837065749674861
9	96.9336240212348	1.6207514919359238	0.5745858838242632	0.8710386030050022
10	58.02907386054189	28.371765267645717	5.507407019167082	8.091753852645308
11	35.19771726446063	27.80885231297603	18.252559198780723	18.74087122378262
12	31.786841070589478	18.300705898069864	25.304066577361596	24.608386453979065
13	24.255321681788015	18.48287634201484	27.199479116203847	30.0623228599933
14	24.139782830609263	22.16894266739989	29.38003519073996	24.311239311250883
15	28.44259646948455	18.70535641949928	27.570598900628323	25.281448210387847
16	28.245148707495904	27.795922807878465	23.32373595900388	20.635192525621747
17	33.82986821494459	20.710719353352793	21.36959502872103	24.089817402981588
18	31.54432191359321	20.55073739513791	21.980191733648226	25.924748957620654
19	28.62142706684422	22.327998727551517	24.089685131829697	24.960889073774567
20	29.107060601020272	24.40597852379442	22.130054948743275	24.35690592644203
21	30.592134958901703	21.340495375304513	24.896109277134848	23.17126038865894
22	30.28662166581627	21.057534313616884	22.118977239772217	26.536866780794625
23	29.645867138257415	24.818366907609594	21.15091774685352	24.38484820727948
24	29.916427779455052	23.612880697042517	20.78687446905533	25.683817054447104
25	27.78444828545173	20.64507979422577	21.541117644938595	30.029354275383906
26	30.099127308007596	21.097612472640538	21.23411630139437	27.569143917957494
27	27.776512016338135	19.68988367082869	22.26100338911759	30.272600923715586
28	28.582208670307875	24.991939726681505	20.159082514382007	26.266769088628617
29	32.978339606843846	22.251049984937623	19.464162950122862	25.306447458095672
30	28.833391587753148	24.044183855578417	21.91253503945483	25.2098895172136
31	33.850568650215884	22.221818060369213	19.792757559213662	24.13485573020124
32	33.543038222064084	22.00479416790037	21.901258923755933	22.550908686279612
33	35.11997489493537	20.85717658628659	21.123901364079323	22.898947154698718
34	30.283579429322728	22.591780472214626	23.8276229121412	23.29701718632144
35	28.672880544930692	28.56620386092879	22.64892160983251	20.111993984308015
36	31.29122106444548	26.40948966152143	20.244132865049366	22.055156408983727
37	30.35398074991791	26.157281642648982	21.203230987427297	22.285506620005812
38	32.894380493179604	26.035393776179355	19.732474981738314	21.337750748902728
39	30.909056638837917	23.992631174128025	19.880188790615097	25.218123396418957
40	29.154876622429683	23.656497109379316	21.925762154644158	25.262864113546847
41	29.050779225889695	23.88949274343927	20.851422791179232	26.208305239491803
42	28.134768431240992	22.58414181319279	21.47627171272293	27.804818042843287
43	26.662788917396995	22.683841193932324	23.940913153737768	26.71245673493291
44	26.47701408456293	21.183919399250883	23.692772472786036	28.64629404340015
45	27.481878025495927	24.154696402985227	22.361099583312804	26.002325988206042
46	27.85445279259123	23.030027866224927	23.97689090705273	25.138628434131117
47	28.103750846122022	22.21579972295807	23.344171851971385	26.33627757894852
48	30.07958424531537	21.87027440642494	23.508088876955092	24.5420524713046
49	28.973003788576467	22.64802877955723	23.811320492670358	24.567646939195946
50	25.825710932640582	23.262692822405146	24.118784785246213	26.79281145970806
51	25.71417328380661	24.485539621658212	23.473070089491355	26.327217005043828
52	28.192868534710097	22.82027888711021	23.30197735451744	25.684875223662253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	17.5
6	35.0
7	77.0
8	119.0
9	361.0
10	603.0
11	8345.5
12	16088.0
13	14641.5
14	12687.5
15	12180.0
16	10697.0
17	9214.0
18	8712.0
19	8210.0
20	9129.0
21	10048.0
22	10034.0
23	10020.0
24	11449.0
25	12878.0
26	15204.5
27	17531.0
28	19347.5
29	21164.0
30	21805.0
31	22446.0
32	25204.5
33	27963.0
34	29914.5
35	31866.0
36	34094.5
37	36323.0
38	39439.5
39	42543.0
40	42530.0
41	47748.5
42	52967.0
43	54019.0
44	55071.0
45	59552.5
46	64034.0
47	72016.0
48	79998.0
49	89043.0
50	98088.0
51	99191.0
52	100294.0
53	123457.5
54	146621.0
55	182384.0
56	218147.0
57	234753.5
58	251360.0
59	282501.5
60	313643.0
61	291188.0
62	268733.0
63	270658.5
64	255060.0
65	237536.0
66	201835.0
67	166134.0
68	140585.0
69	115036.0
70	102926.0
71	90816.0
72	75058.5
73	59301.0
74	57514.0
75	55727.0
76	41264.5
77	26802.0
78	18433.0
79	10064.0
80	7091.0
81	4118.0
82	2835.0
83	1552.0
84	901.0
85	250.0
86	223.5
87	197.0
88	109.5
89	16.0
90	10.0
91	8.0
92	6.0
93	8.5
94	11.0
95	5.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3024091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.2036629993827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45357950770628	9.208100564091758
2	9.239507300373205	2.2551166674818166
3	3.9246639111980097	1.4368582727429924
4	2.0552239825062677	1.003250434830228
5	1.2337671938498183	0.7528239526718625
6	0.7506842383325601	0.5496658478135309
7	0.5155355941305111	0.44039958584687067
8	0.36498503718971265	0.3563323514944332
9	0.2648979213139154	0.2909452464856817
>10	2.5755102753288015	7.722658713731893
>50	1.6472769692967106	14.769987371635619
>100	1.8328976008545075	40.38143324313279
>500	0.09638647264856831	7.973958918837999
>1k	0.04135290600728899	8.932617363208411
>5k	0.002798316947861661	2.3094315281282705
>10k+	9.327723159538869E-4	1.6164199378658044
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	19018	0.6288831916764409	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	12504	0.4134796208182889	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11078	0.36632495516834646	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9409	0.3111348170408893	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	8897	0.29420410959855375	No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8632	0.2854411457856261	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6460	0.21361791030759325	No Hit
GGACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	5622	0.1859071039859581	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5592	0.18491507034675875	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5479	0.18117841030577453	No Hit
GGACATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	5406	0.17876446178372277	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	5367	0.17747481805276363	No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4962	0.1640823639235724	No Hit
GGACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	4313	0.14262136952889315	No Hit
GGACATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	3961	0.13098150816228746	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3839	0.1269472380295434	No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3654	0.12082969725448077	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3641	0.1203998160108277	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3623	0.11980459582730811	No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3559	0.11768825739701615	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3409	0.1127280892010194	No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3299	0.10909063252395515	No Hit
GGACATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	3282	0.10852848012840882	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3233	0.10690815851771655	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3152	0.10422966769187833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.2988124365305147E-4	0.0	0.0	0.0	0.0
8	0.0014880504587990243	0.0	0.0	0.0	0.0
9	0.00459642252829032	0.0	0.0	0.0	0.0
10	0.030157822631660225	0.0	0.0	0.0	0.0
11	0.05638057849449636	0.0	0.0	0.0	0.0
12	0.07645272579429653	0.0	0.0	0.0	0.0
13	0.09401172120812502	0.0	0.0	0.0	0.0
14	0.12780700051684954	0.0	0.0	0.0	0.0
15	0.1492349271235555	0.0	0.0	0.0	0.0
16	0.18064932569820155	0.0	0.0	0.0	0.0
17	0.20108521866570814	0.0	0.0	0.0	0.0
18	0.21950397656684273	0.0	0.0	0.0	0.0
19	0.2412625810532818	0.0	0.0	0.0	0.0
20	0.2622606264163347	0.0	0.0	0.0	0.0
21	0.27948894395043006	0.0	0.0	0.0	0.0
22	0.2977092951237248	0.0	0.0	0.0	0.0
23	0.32085674670504294	0.0	0.0	0.0	0.0
24	0.3458229266248932	0.0	0.0	0.0	0.0
25	0.3655974638329336	0.0	0.0	0.0	0.0
26	0.38821583080667876	0.0	0.0	0.0	0.0
27	0.413810298698022	0.0	0.0	0.0	0.0
28	0.48146699289141764	0.0	0.0	0.0	0.0
29	0.4974718022705005	0.0	0.0	0.0	0.0
30	0.5272989470224275	0.0	0.0	0.0	0.0
31	0.5424770617021776	0.0	0.0	0.0	0.0
32	0.5572914307142213	0.0	0.0	0.0	0.0
33	0.5705516136915192	0.0	0.0	0.0	0.0
34	0.5896647951400933	0.0	0.0	0.0	0.0
35	0.6606282681308201	0.0	0.0	0.0	0.0
36	0.6802705341869673	0.0	0.0	0.0	0.0
37	0.694456615227518	0.0	0.0	0.0	0.0
38	0.7069893068694031	0.0	0.0	0.0	0.0
39	0.719389727359395	0.0	0.0	0.0	0.0
40	0.732550706972773	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	3.418488E-5	46.0	11
CGTCTAT	20	6.313097E-4	46.0	15
CACGATT	20	6.313097E-4	46.0	12
TTATCCG	20	6.313097E-4	46.0	14
CGCCTAT	45	3.110472E-10	46.0	22
CCGTAAA	25	3.418488E-5	46.0	42
AATTCGC	20	6.313097E-4	46.0	38
ACGCTAA	65	0.0	46.0	18
TCATATC	20	6.313097E-4	46.0	16
ATAACGT	50	1.6370905E-11	46.0	38
CGCACTA	30	1.8624178E-6	46.0	37
TACGTAT	30	1.8624178E-6	46.0	30
CGTATAG	140	0.0	44.357143	31
ATGGGCT	10565	0.0	43.3876	5
ATGGGGC	37030	0.0	43.36646	5
ATGGGAG	30165	0.0	43.35422	5
CATGGGG	180970	0.0	43.276398	4
ATGGGGG	68850	0.0	43.193897	5
CTCATAA	245	0.0	43.183674	45
ATGGGGT	25015	0.0	43.14052	5
>>END_MODULE